배추 시스테인 단백질 분해 효소 억제 유전자 BCPI-1의 발현과 종자 발아 및 유묘 생장과의 연관성

홍준기,이은영,김정률,양경애,최영주,정우식,김호일,윤대진,이상열,조무제,임체오 Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-

Phytocystatins are protein inhibitors of cysteine proteinases of the papain family that have been identified in both monocot and dicot plants. A cDNA encoding a phytocystatin, BCPI-1 (Brassica Cysteine Proteinase Inhibitor-1) has been isolated from Chinese cabbage (Brassica campestris L. ssp. pekinensis) flower buds. Here, we tested whether BCPI-1 transcription is regulated by hormones, and could be involved in regulating cysteine proteinases during and after germination. BCPI-1 was sensitive to exogenous GA_(3) and ABA, which are important factors controlling seed germination, suggesting that the expression of BCPI-1 is hormonally regulated. We introduced a recombinant plasmid containing the full-length BCPI-1 cDNA under the control of cauliflower mosaic virus 35S promoter into rice embryogenic calli using the particle delivery method, and regenerated a number of transgenic rice plants. Constitutively over-expressed BCPI-1 caused changes in overall plant growth and development, including reduced germination and seedling growth. These data support the role of the BCPI-1 in the regulation of endogenous proteinases during both seed germination and subsequent seedling development. Phylocystatins은 papain계열의 cysteine 단백질 분해 효소 활성을 특이적으로 억제하는 억제자로 다양한 식물 종으로부터 분리되었다. 본고에서는, 배추 화아 cDNA library로부터 분리된 phytocystatin인 BCPI-1(Brassica Cysteine Proteinase Inhibitor-1)의 생체 내 기능에 대해 연구하였다. 먼저, BCPI-1 전사체는 발아 조절에 중요한 영향을 미치는 GA₃와 ABA에 의해 예민하게 증가, 혹은 감소되는 반응을 보임으로써, BCPI-1이 식물 호르몬의 영향을 받으며, 특히 발아나 유묘의 생장 조절에 관련이 있음을 알 수 있었다. Particle bombardment 방법을 통하여 BCPI-1을 벼의 배 형성 세포 내에 도입, 재분화 시켜 형질 전환 벼를 생성하였다. CaMV 35S promoter의 조절에 의해 지속적인 BCPI-1 발현을 보이는 형질 전환 벼의 경우, 발아와 유묘 생장이 현저히 지연되었다. 위의 결과들을 바탕으로, BCPI-1이 식물체 내에서 생성되는 cysteine 계열의 단밸질 분해 효소 활성을 조절하여 종자의 발아와 유묘의 생장에 영향을 미치는 것으로 추측한다.

Baicalin Induces Apoptosis in Leukemia HL-60/ADR Cells via Possible Down-regulation of the PI3K/Akt Signaling Pathway

Zheng, Jing,Hu, Jian-Da,Chen, Ying-Yu,Chen, Bu-Yuan,Huang, Yi,Zheng, Zhi Hong,Liu, Ting-Bo Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4

Background: The effect and possible mechanism of traditional Chinese medicine, baicalin, on the PI3K/Akt signaling pathway in drug-resistant human myeloid leukemia HL-60/ADR cells have been investigated in this current study. Methods: HL-60/ADR cells were treated by 20, 40, $80\;{\mu}mol/L$ baicalin followed by cell cycle analysis at 24h. The mRNA expression level of the apoptosis related gene, Bcl-2 and bad, were measured by RT-PCR on cells treated with $80\;{\mu}mol/L$ baicalin at 12, 24 and 48hr. Western blot was performed to detect the changes in the expression of the proteins related to HL-60/ADR cell apoptosis and the signaling pathway before and after baicalin treatment, including Bcl-2, PARP, Bad, Caspase 3, Akt, p-Akt, NF-${\kappa}B$, p-NF-${\kappa}B$, mTOR and p-mTOR. Results: Sub-G1 peak of HL-60/ADR cells appeared 24 h after $20\;{\mu}mol/L$ baicalin treatment, and the ratio increased as baicalin concentration increased. Cell cycle analysis showed 44.9% G0/G1 phase cells 24 h after baicalin treatment compared to 39.6% in the control group. Cells treated with $80\;{\mu}mol/L$ baicalin displayed a trend in decreasing of Bcl-2 mRNA expression over time. Expression level of the Bcl-2 and PARP proteins decreased significantly while that of the PARP, Caspase-3, and Bad proteins gradually increased. No significant difference in Akt expression was observed between treated and the control groups. However, the expression levels of p-Akt, NF-${\kappa}B$, p-NF-${\kappa}B$, mTOR and p-mTOR decreased significantly in a time-dependent manner. Conclusions: We conclude that baicalin may induce HL-60/ADR cell apoptosis through the PI3K/AKT signaling pathway.

Three Dimensional Simulation of Viscoelastic Polymer Melts Flow in a Cast Film Process

Zheng, Hong,Yu, Wei,Zhou, Chixing,Zhang, Hongbin The Korean Fiber Society 2007 Fibers and polymers Vol.8 No.1

Three-dimensional simulation of the cast film process for viscoelastic polymer materials was carried out using the finite element method. The flow between the extrusion die and chill roll was assumed to be steady-state and isothermal and the rheological property of material was characterized by a single-mode PTT model. Gravity and inertial flow were considered in the simulation work, but neglecting die swell at the die exit, surface tension, crystallization, transient disturbance and film sag. Simulation results of the cast film production line were compared with the experimental data on the velocity profiles and neck-in values. Neck-in and edge bead was well predicted and the influence of strain-hardening nature, elasticity of materials and operation conditions on the final film shape was also investigated. It was found that the effect of strain-hardening nature was masked for the material with a very low relaxation time when the single-mode PTT model was adopted. Greater elasticity helped to produce a film with smaller neck-in and less waste edge. When the air gap length was increased, it was predicted that neck-in phenomenon would be promoted as well. Moreover, it was found that ratio of neck-in values under different air gap lengths was approximately equal to that of air gap lengths, which was consistent with experimental evidence.

Effects of SRBSDV-infected rice plants on the fitness of vector and non-vector rice planthoppers

Hong-Xing Xu,Hong-Xing Xu,Xu-Song Zheng,Yajun Yang,Jue-Feng Zhang Zhang,Zhong-Xian Lu 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.3

Southern rice black-streaked dwarf virus (SRBSDV) is a new member of the genus Fijivirus and transmitted by whitebacked planthopper (WBPH), Sogatella furcifera (Horváth). The rice area affected by SRBSDV and the subsequent damages are increasing rapidly. In current study, we evaluated the effects of SRBSDV-infected plants on the ecological fitness and the tolerance to adverse environmental factors of vector WBPH and non-vector brown planthopper (BPH), Nilaparvata lugens (Stål). Our study revealed that the fitness of WBPH and BPH were both influenced by feeding on SRBSDV-infected rice. Fecundity ofWBPH feeding on the SRBSDV-infected rice plants were higher than those on healthy plants, nymphal duration of male WBPH prolonged markedly by 0.77 d (P b 0.05), while the weight of brachypterous female adults significantly decreased (P b 0.05), and the average survival time shortened both at 26 °C and 31 °C, respectively. Median lethal time of WBPH on SRBSDV-infected and healthy rice plants were 80 h and 112 h at 26 °C, 64 h and 88 h at 31 °C, respectively. As for non-vector BPH, nymph survival rate decreased significantly by 11.22% (P b 0.05) and the longevity of female adults also shortened (P b 0.05), while hatchability of BPH eggs increased (P b 0.05). Survival time of BPH feeding on healthy rice at 26 °Cwas significant longer than those feeding on healthy and infected rice at 31 °C. The above results imply that the vectorWBPH suffers much stronger effects than non-vector BPH fromfeeding on SRBSDVinfected rice plants.

Isolation, Purification, and Characterization of a Thermostable Xylanase from a Novel Strain, Paenibacillus campinasensis G1-1

( Hong Chen Zheng ),( Yi Han Liu ),( Xiao Guang Liu ),( Jian Ling Wang ),( Ying Han ),( Fu Ping Lu ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.7

High levels of xylanase activity (143.98 IU/ml) produced by the newly isolated Paenibacillus campinasensis G1-1 were detected when it was cultivated in a synthetic medium. A thermostable xylanase, designated XynG1-1, from P. campinasensis G1-1 was purified to homogeneity by Octyl-Sepharose hydrophobic-interaction chromatography, Sephadex G75 gel-filter chromatography, and Q-Sepharose ion-exchange chromatography, consecutively. By multistep purification, the specific activity of XynG1-1 was up to 1,865.5 IU/mg with a 9.1-fold purification. The molecular mass of purified XynG1-1 was about 41.3 kDa as estimated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Sequence analysis revealed that XynG1-1 containing 377 amino acids encoded by 1,134 bp genomic sequences of P. campinasensis G1-1 shared 96% homology with XylX from Paenibacillus campinasensis BL11 and 77%~78% homology with xylanases from Bacillus sp. YA- 335 and Bacillus sp. 41M-1, respectively. The activity of XynG1-1 was stimulated by Ca2+, Ba2+, DTT, and β- mercaptoethanol, but was inhibited by Ni2+, Fe2+, Fe3+, Zn2+, SDS, and EDTA. The purified XynG1-1 displayed a greater affinity for birchwood xylan, with an optimal temperature of 60oC and an optimal pH of 7.5. The fact that XynG1-1 is cellulose-free, thermostable (stability at high temperature of 70oC~80oC), and active over a wide pH range (pH 5.0~9.0) suggests that the enzyme is potentially valuable for various industrial applications, especially for pulp bleaching pretreatment.

Improved Mutual MRAS Speed Identification Based on Back-EMF

Hong Zheng,Jiancheng Zhao,Liangzhong Liu 대한전기학회 2016 Journal of Electrical Engineering & Technology Vol.11 No.3

In the design of sensorless control system for induction motor, high-precision speed estimation is one of the most difficult problems. To solve this problem, the common method is model reference adaptive method (MRAS). MRAS requires accurate motor parameters to estimate rotor speed precisely. However, when motor is running, the variety of temperature and magnetic saturation will lead to the change of motor parameters such as stator resistance and rotor resistance, which will lower the accuracy of the speed estimation. To improve the accuracy and rapidity of speed estimation, this paper analyses the mutual MRAS speed identification based on rotor flux linkage, and proposes an improved mutual MRAS speed identification based on back-EMF. The improved method is verified by Simulink simulation and motor experimental platform based on DSP2812. The results of simulation and experiment indicate that the method proposed by this paper can significantly improve the accuracy of speed identification, and speed up the response of identification.

Build modern logistic service chain based on logistic simulation

Hong-Xing Zheng,Xiang-Wen Li 한국멀티미디어학회 2006 한국멀티미디어학회 국제학술대회 Vol.2006 No.-

To the logistic enterprises alliance that provide the modem logistic services, this paper firstly builds the integrated resources data warehouse based on the logistic resources meta data of the enterprises alliances. It also analyzes the primary factors that affect the enterprises alliance to build logistic services chain, and gives out the enterprises alliance services pattern that is based on the multi-layer service broker. However, the logistic resources scheduling rules based on market is given out. At last, adopting the above solution, this paper gives out the simulation process of an example, which is about one virtual enterprises alliance how to build one timely logistic service chain, and simulate by using ProModel software.

Combining a HMM with a Genetic Algorithm for the Fault Diagnosis of Photovoltaic Inverters

Zheng, Hong,Wang, Ruoyin,Xu, Wencheng,Wang, Yifan,Zhu, Wen The Korean Institute of Power Electronics 2017 JOURNAL OF POWER ELECTRONICS Vol.17 No.4

The traditional fault diagnosis method for photovoltaic (PV) inverters has a difficult time meeting the requirements of the current complex systems. Its main weakness lies in the study of nonlinear systems. In addition, its diagnosis time is long and its accuracy is low. To solve these problems, a hidden Markov model (HMM) is used that has unique advantages in terms of its training model and its recognition for diagnosing faults. However, the initial value of the HMM has a great influence on the model, and it is possible to achieve a local minimum in the training process. Therefore, a genetic algorithm is used to optimize the initial value and to achieve global optimization. In this paper, the HMM is combined with a genetic algorithm (GHMM) for PV inverter fault diagnosis. First Matlab is used to implement the genetic algorithm and to determine the optimal HMM initial value. Then a Baum-Welch algorithm is used for iterative training. Finally, a Viterbi algorithm is used for fault identification. Experimental results show that the correct PV inverter fault recognition rate by the HMM is about 10% higher than that of traditional methods. Using the GHMM, the correct recognition rate is further increased by approximately 13%, and the diagnosis time is greatly reduced. Therefore, the GHMM is faster and more accurate in diagnosing PV inverter faults.

Click Synthesis of Hydrogels by Metal-Free 1,3-Dipolar Cycloaddition Reaction Between Maleimide and Azide Functionalized Polymers

Hong-Zheng Zhu,Gang Wang,Hong-Liang Wei,Hui-Juan Chu,Jing Zhu 한국고분자학회 2016 Macromolecular Research Vol.24 No.9

Herein, a metal-free 1,3-dipolar cycloaddition click reaction was used to prepare hydrogels. First, copolymer of N,N-dimethylacrylamide and 2-hydroxyethyl methacrylate was synthesized via free radical copolymerization with an initiator of 2,2'-azobisisobutyronitrile and a solution of 1,4-dioxane. Second, maleimide-functionalized polymer was obtained by a coupling reaction of the above synthesized copolymer and N-maleoyl alanine with a dehydrating agent of N,N'-dicyclohexylcarbodiimide. Similarly, azido-modified polymer was prepared by the reaction between azidoacetic acid and the as-prepared copolymer. Third, the hydrogels were obtained by the reaction of the azidomodified polymer and maleimide-functionalized copolymer without any initiator or catalyst. Gelation times of different conditions were measured by a cuvette inversion method. The samples of different steps were analysized by means of FTIR, NMR, SEM, etc. The swelling behaviour of the gels was investigated via a general gravimetric method. Finally, release mechanism was explored with a model drug molecule of 5-fluorouracil. It was found temperature, concentration and solvent had an impact on the gelation time. And the swelling behavior and drug-controlled release profiles of the hydrogels were relevant to the concentration of polymers used for the hydrogels.

Tricuspone, a Rearranged Diterpenoid from Salvia tricuspis

Hong-Bo Zheng,Teng Zhang,노경호,Wei-Dong Xie 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.4