Non-Benzoquinone Geldanamycin Analog, WK-88-1, Induces Apoptosis in Human Breast Cancer Cell Lines

( Yu-ru Zhao ),( Hong-mei Li ),( Meilin Zhu ),( Jing Li ),( Tao Ma ),( Qiang Huo ),( Young-soo Hong ),( Cheng-zhu Wu ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.4

Heat shock protein 90 (Hsp90) is treated as a molecular therapeutic target for the prevention and treatment of cancer. Geldanamycin (GA) was the first identified natural Hsp90 inhibitor, but hepatotoxicity has limited its clinical application. Nevertheless, a new GA analog (WK-88- 1) with the non-benzoquinone skeleton, obtained from genetically engineered Streptomyces hygroscopicus, was found to have anticancer activity against two human breast cancer cell lines. WK-88-1 produced concentration-dependent inhibition of cell proliferation, cell cycle arrest, and apoptosis in estrogen receptor (ER)-positive MCF-7 and ER-negative MDA-MB-231 cell lines. Detailed analysis showed that WK-88-1 downregulated some key cell cycle molecules (CDK1 and cyclin B1) and lead to G2/M cell cycle arrest. Further studies also showed that WK- 88-1 could induce human breast cancer cell apoptosis by downregulating Hsp90 client proteins (Akt, p-Akt, IKK, c-Raf, and Bcl-2), decreasing the ATP level, increasing reactive oxygen species production, and lowering the mitochondrial membrane potential. Meanwhile, we discovered that WK-88-1 significantly decreased the levels of Her-2 and ER-α in MCF-7 cells but not in MDA-MB-231 cells. In addition, WK-88-1 significantly increased caspase-3, -8, and -9 activities and the cleavage of PARP in a concentration-dependent manner (with the exception of caspase-3 and PARP in MCF-7 cells). Taken together, our preliminary results suggest that WK-88-1 has the potential to play a role in breast cancer therapy.

The Application Strategy of the Palm-toy Cultural Connotation for Chinese Elderly

Chen Hong-Ru,Bum-Kyu Kang 한국디자인학회 2015 디자인학연구 Vol.28 No.4

좌심실 이완기능의 평가에 있어서 도플러 조직영상으로 평가한 승모판륜 속도의 유용성

홍그루(Gue Ru Hong),전대진(Dae Jin Jun),배준호(Jun Ho Bae),이종석(Jong Suk Lee),김형준(Hyung Jun Kim),박종선(Jong Sun Park),신동구(Dong Gu Shin),김영조(Young Jo Kim),심봉섭(Bong Sup Shim) 대한내과학회 1999 대한내과학회지 Vol.57 No.6

N/A Background : Doppler echocardiography is widely used for the noninvasive evaluation of left ventricular diastolic function. However the mitral flow velocity pattern is affected by several physiologic factors. The mitral annular velocity profile by Doppler tissue imaging may provide more additional information about left ventricular diastolic function. Thus, this study designed to assess the relationship between cardiac catheterization, MUGA scan, mitral flow velocity, and mitral annular velocity data and to assess the clinical availavility of mitral annulus velocity in the evaluation of left ventricular diastolic function. Methods : The study population consisted of 20 patients with dilated cardiomyopathy( 64±7years), 20 patients with normal left ventricular function (61±7years). Left ventricular catheterization was performed with fluid-filled catheter and left ventricular end diastolic pressure, -dP/dtmax were measured. The mitral flow velocity was recorded at mitral valve tip and the mitral annulus velocity during diastole was measured by Doppler tissue imaging(DTI). Simultaneously EF(ejection fraction), PER(Peak filling rate), PFR(Peak filling rate) were measured by MUGA blood pool scan. Results : Mean peak E velocity, mean peak A velocity, E/A ratio, mean peak E' velocity, mean peak A' velocity, E'/A' ratio and -dP/dtmax significantly difference betweeen two group. -dP/dtmax by cardiac catheterization showed significant correlation with mean peak E' velocity (r=0.552, p=0.003), E'/A' ratio(r=0.507, p=0.003), DT of E'(r=-0.556, p=0.001), TVI of E'(r=0.689, p<0.001) and DT of E wave(r=-0.538, p=0.003). PFR by MUGA scan also showed significant correlation with -dP/dtmax(r=0.537, p=0.01). Conclusion : Among mitral annulus velocity index mean peak E' velocity, E'/A' ratio, DT of E',TVI of E' had significant correlation with -dP/dtmax. And DT by mitral flow velocity, PFR by MUGA scan also had significant correlation with -dP/dtmax. Mitral annulus velocity determined by DTI is relatively convenient, safe, and preload-independent variable in evaluating diastole function. Thus mitral annulus velocity by Doppler tissue imaging is may be useful diagnostic modality for evaluating left ventricular diastolic function.(Korean J Med 57:1021-1029, 1999)

핵의학 의사를 위한 심초음파의 최신지견

홍그루 ( Geu Ru Hong ),신동구 ( Dong Gu Shin ) 대한핵의학회 2006 핵의학 분자영상 Vol.39 No.6

어머니의 양육행동 및 양육스트레스와 유아의 자아존중감 간의 관계

김세루(Kim Se-Ru),홍지명(Hong Ji-Myeong),홍혜경(Hong Haekyung) 한국어린이미디어학회 2010 어린이미디어연구 Vol.9 No.2

본 연구는 어머니의 양육행동 및 양육스트레스와 유아의 자아존중감 간의 관계를 밝히고자 하였다. G시에 소재한 어린이집과 유치원 10개소의 만 5세 반 어머니 189명과 그들의 유아 189명을 대상으로 하였으며 어머니의 양육행동, 양육스트레스에 관한 설문조사를 실시하였고, 유아의 자아존중감은 연구자가 유아와 일대일 면접으로 실시하였다. 어머니의 양육행동 및 양육스트레스와 유아의 자아존중감 간의 관계를 알아보기 위해 Pearson 적률상관계수를 산출하고, 이들 변인의 영향력을 알아보기 위해 단계적 회귀분석을 실시하였다. 연구결과, 어머니의 양육행동과 유아의 자아존중감은 정적상관이 나왔으며, 어머니의 양육행동 하위영역인 반응성, 합리적 지도, 애정성 순으로 유의한 정적상관이 나왔다. 그러나 어머니의 양육스트레스와 유아의 자아존중감은 부적상관이 나왔다. 한편 자아존중감에 대한 어머니의 양육행동 및 양육스트레스는 영향력이 있는 것으로 나타났다. 따라서 본 연구결과는 유아의 자아존중감에 영향을 미치는 어머니 관련변인이 양육행동과 양육스트레스임을 밝혔으며, 부모교육 및 유아들의 자아존중감 교육에 시사하는 바 크다. This study was aimed to explore the relations among mother's behavior toward rearing, mother's rearing-stress and self-esteem of young children. The subjects consisted of 189 five-years-old children and their mother. Data were analyzed by Pearson’s r and hierarchical multiple regression analysis. The results of study were as follows; First, there were significant correlations between mother's behavior toward rearing and self-esteem of young children. Second, there were significant correlations between mother's rear-stress and self-esteem of young children. Third, mother's behavior toward rearing and mother's rearing-stress are reliable predictors of self-esteem of young children. The educational implications of these indicate that we need to actively develop programs to increase self-esteem of young children through parents education

Comparison of Transcatheter Aortic Valve Replacement between Self-Expanding versus Balloon-Expandable Valves in Patients with Small Aortic Annulus

Yong-Joon Lee,Seung-Jun Lee,Sung-Jin Hong,Chi Young Shim,Chul-Min Ahn,Jung-Sun Kim,Byeong-Keuk Kim,Geu-Ru Hong,Young-Guk Ko,Donghoon Choi,Yangsoo Jang,Myeong-Ki Hong 대한심장학회 2021 Korean Circulation Journal Vol.51 No.3

Background and Objectives: Transcatheter aortic valve replacement (TAVR) has been reported as a good alternative for surgical aortic valve replacement in patients with small aortic annulus. Head-to-head comparisons of different transcatheter aortic valves in these patients are insufficient. We compared the outcomes after TAVR between two different types of recent transcatheter aortic valves (self-expanding vs. balloon-expandable) in patients with small aortic annulus. Methods: A total of 70 patients with severe aortic stenosis and small annulus (mean diameter ≤23 mm or minimal diameter ≤21 mm on computed tomography) underwent TAVR with either a self-expanding valve with supra-annular location (n=45) or a balloon-expandable valve with intra-annular location (n=25). The echocardiographic hemodynamic parameters after TAVR and 1-year follow-up were compared. Results: Between the self-expanding and balloon-expandable valve-treated patients, the clinical outcomes including permanent pacemaker implantation (11.1% vs. 8.0%), acute kidney injury stage 2 or 3 (4.4% vs. 4.0%), and major vascular complication (4.4% vs. 0.0%) were similar without all-cause mortality, stroke, and life-threatening bleeding during 30-day follow-up. Compared with the balloon-expandable valve-treated patients, the self-expanding valve-treated patients presented larger effective orifice area (EOA) (1.46±0.28 vs. 1.75±0.42 cm2, p=0.002) and indexed EOA (0.95±0.21 vs. 1.18±0.28 cm2/m2, p=0.001), whereas mean aortic valve gradient (11.7±2.9 vs. 8.9±5.2 mmHg, P=0.005) and incidence of ≥moderate prosthesis-patient mismatch (36.0% vs. 8.9%, p=0.009) were lower. These hemodynamic differences were maintained at 1-year follow-up. Conclusions: TAVR with self-expanding valves was associated with superior hemodynamic outcomes compared with balloon-expandable valves in patients with small aortic annulus.

Comparison of Intracardiac Echocardiography Versus Transesophageal Echocardiography for Guidance During Transcatheter Aortic Valve Replacement

Sang-Hyup Lee,Seunguk Oh,Young-Guk Ko,Yong-Joon Lee,Seung-Jun Lee,Sung-Jin Hong,Chul-Min Ahn,Jung-Sun Kim,Byeong-Keuk Kim,Kyu-Yong Ko,Iksung Cho,Chi Young Shim,Geu-Ru Hong,Donghoon Choi,Myeong-Ki Hong 대한심장학회 2024 Korean Circulation Journal Vol.54 No.2

Background and Objectives: Evidence regarding the efficacy and safety of intracardiac echocardiography (ICE) for guidance during transcatheter aortic valve replacement (TAVR) is limited. This study aimed to compare the clinical efficacy and safety of ICE versus transesophageal echocardiography (TEE) for guiding TAVR. Methods: This prospective cohort study included patients who underwent TAVR from August 18, 2015, to June 31, 2021. Eligible patients were stratified by echocardiographic modality (ICE or TEE) and anesthesia mode (monitored anesthesia care [MAC] or general anesthesia [GA]). Primary outcome was the 1-year composite of all-cause mortality, rehospitalization for cardiovascular cause, or stroke, according to the Valve Academic Research Consortium-3 (VARC-3) definition. Propensity score matching was performed, and study outcomes were analyzed for the matched cohorts. Results: Of the 359 eligible patients, 120 patients were matched for the ICE-MAC and TEE-GA groups, respectively. The incidence of primary outcome was similar between matched groups (18.3% vs. 20.0%; adjusted hazard ratio, 0.94; 95% confidence interval [CI], 0.53–1.68; p=0.843). ICE-MAC and TEE-GA also had similar incidences of moderate-to-severe paravalvular regurgitation (PVR) (4.2% vs. 5.0%; adjusted odds ratio, 0.83; 95% CI, 0.23–2.82; p=0.758), new permanent pacemaker implantation, and VARC-3 types 2–4 bleeding. Conclusions: ICE was comparable to TEE for guidance during TAVR for the composite clinical efficacy outcome, with similar incidences of moderate-to-severe PVR, new permanent pacemaker implantation, and major bleeding. These results suggest that ICE could be a safe and effective alternative echocardiographic modality to TEE for guiding TAVR.

Dextromethorphan Protect the Valproic Acid Induced Downregulation of Neutrophils in Patients with Bipolar Disorder

Ru-Band Lu,Yun-Hsuan Chang,Sheng-Yu Lee,Tzu-Yun Wang,Shu-Li Cheng,Po See Chen,Yen Kuang Yang,Jau-Shyong Hong,Shiou-Lan Chen 대한정신약물학회 2020 CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE Vol.18 No.1

Objective: Valproic acid (VPA) is an anticonvulsant and commonly long term used as a mood stabilizer for patients with mood disorders. However its chronic effects on the hematological changes were noticed and need to be further evaluated. In this study, we evaluated, in Taiwanese Han Chinese patients with bipolar disorders (BD), the chronic effects of VPA or VPA plus dextromethorphan (DM) on the hematological molecules (white blood cell [WBCs], red blood cells [RBCs], hemoglobin, hematocrit, and platelets). Methods: In a 12-week, randomized, double-blind study, we randomly assigned BD patients to one of three groups: VPA plus either placebo (VPA+P, n = 57) or DM (30 mg/day, VPA+DM30, n = 56) or 60 mg/day (VPA+DM60, n = 53). The Young Mania Rating Scale and Hamilton Depression Rating Scale were used to evaluate symptom severity, and the hematological molecules were checked. Results: Paired t test showed that the WBC, neutrophils, platelets and RBCs were significantly lowered after 12 weeks of VPA+P or VPA+DM30 treatment. VPA+DM60 represented the protective effects in the WBCs, neutrophils, and RBCs but not in the platelets. We further calculated the changes of each hematological molecules after 12 weeks treatment. We found that combination use of DM60 significantly improved the decline in neutrophils induced by the long-term VPA treatment. Conclusion: Hematological molecule levels were lower after long-term treatment with VPA. VPA+DM60, which yielded the protective effect in hematological change, especially in the neutrophil counts. Thus, DM might be adjunct therapy for maintaining hematological molecules in VPA treatment.

승모판 질환에 동반된 심방세동에 대한 고주파 절제술을 이?한 수술 중 폐정맥 분리술의 유용성

홍그루,신동구,박종선,김영조,심봉섭,이장훈,정태은,이동협,한승세 대한순환기학회 2002 Korean Circulation Journal Vol.32 No.7

A Spraying Path Planning Algorithm Based on Point Cloud Segmentation and Trajectory Sequence Optimization

Ru-Xiang Hua,Hong-Xuan Ma,Wei Zou,Wei Zhang,Zhuo Wang 제어·로봇·시스템학회 2024 International Journal of Control, Automation, and Vol.22 No.2

Spraying trajectory planning is a key and challenging work for intelligent spraying robot. In order to effectively fulfill spraying on complex surfaces without CAD model, a novel spraying trajectory planning method based on segmentation and trajectory sequence optimization is proposed in this paper, which is mainly composed by three steps: surface segmentation, trajectories generation and trajectories connection. In surface segmentation, a method named regional growth with minimum curvature point (RGMCP) is proposed to segment a 3D entity into different subsurfaces by taking normals and curvatures into consideration simultaneously. In trajectories generation step, an intersection of plane and point cloud (IPPC) algorithm is used to generate the optimal spraying trajectory for each segmented subsurface. Finally, for trajectories connection, a sequence optimization algorithm based on swap-evolution particles (SOSP) is proposed to connect all the subsurface trajectories as a complete spraying one in an optimum manner by regarding it as a sequence optimization problem. The effectiveness of the proposed method is validated by simulation and practical experiment simultaneously. Comparatively, our method can improve the efficiency of a spray task with 367 trajectories and 627 s time-consuming to 215 trajectories and 413 s, while the coating thickness variances are lowered from 51.9 µm2 and 30.4 µm2 to 3.64 µm2 and 7.89 µm2 respectively, which shows that the proposed method is more effective and can keep better coating thickness uniformity.