Curcumin protects retinal pigment epithelial cells against oxidative stress via induction of heme oxygenase-1 expression and reduction of reactive oxygen

Woo, Je Moon,Shin, Da-Yong,Lee, Sung Ju,Joe, Yeonsoo,Zheng, Min,Yim, Jin Ho,Callaway, Zak,Chung, Hun Taeg Molecular Vision 2012 Molecular vision Vol.18 No.-

<P><B>Purpose</B></P><P>To determine whether curcumin induces expression of the defensive enzyme heme oxygenase-1 (HO-1) and protects cells against oxidative stress in cultured human retinal pigment epithelial cells.</P><P><B>Methods</B></P><P>Effective concentrations and toxicities of curcumin were determined after 3 h of curcumin treatment with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Confluent human retinal pigment epithelium cell lines (ARPE-19) were preincubated with curcumin and oxidatively challenged with H<SUB>2</SUB>O<SUB>2</SUB>. HO-1 expression was determined with western blot analysis. To confirm the protective role of HO-1 in oxidative stress, small interfering RNA (siRNA) against HO-1 or inhibitor of HO-1 was treated with curcumin in retinal pigment epithelium cells. Intracellular levels of reactive oxygen species (ROS) were measured with flow cytometry and confocal microscopy. Apoptosis was evaluated with Annexin V-fluoroscein isothiocyanate staining.</P><P><B>Results</B></P><P>Curcumin had little cytotoxicity at concentrations less than 30 μM, and HO-1 expression was the highest at the 15 μM concentration. At this concentration, curcumin also increased the cytoprotective effect against the oxidative stress of H<SUB>2</SUB>O<SUB>2</SUB> through the reduction of ROS levels in human retinal pigment epithelial cells. Curcumin’s effect on the reduction of ROS was mediated by the increase in HO-1 expression.</P><P><B>Conclusions</B></P><P>Curcumin upregulated the oxidative stress defense enzyme HO-1 and may protect human retinal pigment epithelial cells against oxidative stress by reducing ROS levels.</P>

Eriodictyol Protects Endothelial Cells against Oxidative Stress-Induced Cell Death through Modulating ERK/Nrf2/ARE-Dependent Heme Oxygenase-1 Expression

Lee, Seung Eun,Yang, Hana,Son, Gun Woo,Park, Hye Rim,Park, Cheung-Seog,Jin, Young-Ho,Park, Yong Seek MDPI 2015 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.16 No.7

<P>The pathophysiology of cardiovascular diseases is complex and may involve oxidative stress-related pathways. Eriodictyol is a flavonoid present in citrus fruits that demonstrates anti-inflammatory, anti-cancer, neurotrophic, and antioxidant effects in a range of pathophysiological conditions including vascular diseases. Because oxidative stress plays a key role in the pathogenesis of cardiovascular disease, the present study was designed to verify whether eriodictyol has therapeutic potential. Upregulation of heme oxygenase-1 (HO-1), a phase II detoxifying enzyme, in endothelial cells is considered to be helpful in cardiovascular disease. In this study, human umbilical vein endothelial cells (HUVECs) treated with eriodictyol showed the upregulation of HO-1 through extracellular-regulated kinase (ERK)/nuclear factor erythroid 2-related factor 2 (Nrf2)/antioxidant response element (ARE) signaling pathways. Further, eriodictyol treatment provided protection against hydrogen peroxide-provoked cell death. This protective effect was eliminated by treatment with a specific inhibitor of HO-1 and RNA interference-mediated knockdown of HO-1 expression. These data demonstrate that eriodictyol induces ERK/Nrf2/ARE-mediated HO-1 upregulation in human endothelial cells, which is directly associated with its vascular protection against oxidative stress-related endothelial injury, and propose that targeting the upregulation of HO-1 is a promising approach for therapeutic intervention in cardiovascular disease.</P>

비소세포 폐암종에서 Cyclin D1, p21 및 p53단백의 발현과 예후와의 관계

양석우,조상호,양우익,정우희,안철민,이두연 대한병리학회 1999 Journal of Pathology and Translational Medicine Vol.33 No.12

Thermoelectric Properties of Cu-doped Bi2−xSbxTe3 Prepared by Encapsulated Melting and Hot Pressing

Woo‑Jin Jung,Il‑Ho Kim 대한금속·재료학회 2018 METALS AND MATERIALS International Vol.24 No.2

P-type Bi2−xSbxTe3:Cum (x = 1.5–1.7 and m = 0.002–0.003) solid solutions were synthesized using encapsulated meltingand were consolidated using hot pressing. The effects of Sb substitution and Cu doping on the charge transport and thermoelectricproperties were examined. The lattice constants decreased with increasing Sb and Cu contents. As the amount ofSb substitution and Cu doping was increased, the electrical conductivity increased, and the Seebeck coefficient decreasedowing to the increase in the carrier concentration. All specimens exhibited degenerate semiconductor characteristics andpositive Hall and Seebeck coefficients, indicating p-type conduction. The increased Sb substitution caused a shift in the onsettemperature of the intrinsic transition and bipolar conduction to higher temperatures. The electronic thermal conductivityincreased with increasing Sb and Cu contents owing to the increase in the carrier concentration, while the lattice thermalconductivity slightly decreased due to alloy scattering. A maximum figure of merit, ZTmax= 1.25, was achieved at 373 Kfor Bi0.4Sb1.6Te3:Cu0.003.

冠岳樹木圓地域內 荒廢山地土壤의 肥沃化를 통한 綠化促進에 關한 硏究(II)

禹保命,權台鎬,李宗學,金景河,李峻雨,麻鎬燮 서울大學校 農科大學 1986 서울대농학연구지 Vol.11 No.2

The shallow soil depth and severe rock exposures on the hillslopes in the Kwanak aboretum area which resulted from the heavy soil erosion have made very poor growth of forest vegetation. In order to establish the forest vegetation in this area, it is urgent to recover the fertility of soil and the productivity of existing trees. For this reason, fertilization experiment (using the briquet compound fertilizer) was conducted on summer and autumn season in 1983 with the 4 main native species (Pinus densiflora, Pinus rigida, Juniperus rigida, Rhododendron schlippenbachii) growing on these eroded hillslopes. Result in 1985 and 1986 of this experiment represented that the fertilization was effective toward both height and root-collar diameter growth of trees and spring fertilization was, relatively, more effective than autumn fertilization. It also represented that effects of fertilization to P. rigida were more than those to other species. Therefore, besides the engineering methods and afforestation measures for soil erosion control, rapid establishment of vegetation through conservation and recovery of existing trees by fertilization is available measures for the rehabilitation of rockily eroded hillslopes like Mt. Kwanak area.

A Korean Perspective on Mathematics Education

Woo,Jeong Ho(Jeong Ho Woo) 대한수학교육학회 1992 수학교육학연구 Vol.2 No.1

전직 관료 영입이 기업성과에 미치는 영향: 산업에 따른 효과성 분석

박준호(Park, Jun-Ho),이장우(Lee, Jang-Woo) 한국경영교육학회 2021 경영교육연구 Vol.36 No.1

[연구목적] 지금껏 전직 관료 영입과 기업성과 간의 관계를 다룬 다양한 연구가 진행되어 왔다. 하지만, 선행연구들은 서로 다른 연구방법과 연구대상으로 인해 혼재된 결과를 보임으로써 지속적인 의문을 증폭시키고 있다. 이에 본 연구는 두 가지 산업을 비교 분석함으로써 전직 관료 영입과 기업성과 간의 보다 명확한 관계를 관찰하고자 하였다. [연구방법] 연구를 위해 1998년부터 2017년까지 총20년의 연구기간을 선정하고 의약품 및 의료기기 제조업 189개 기업과 화학산업에 포함된 화학물질 및 화학제품 제조업 136개 기업의 데이터를 수집하여 연구를 진행하였다. [연구결과] 연구결과, 취약한 제도적 환경으로 평가되는 의료산업에서는 전직 관료 영입이 기업성과에 정(+)의 영향을 미치는 것으로 관찰되었으며 비교적 안정적인 제도 환경으로 평가되는 화학산업에서 전직 관료 영입이 기업성과에 유의미한 관계가 없는 것으로 관찰되었다. [연구의 시사점] 본 연구는 그동안 전직 관료 영입과 기업성과 간의 혼재된 연구결과를 설명하는데 기여할 수 있을 것으로 예상된다. 특히, 기업이 속해 있는 산업의 제도적 환경에 따라 전직 관료 영입이 기업성과에 미치는 영향이 다를 수 있음을 시사하고 있다. [Purpose] So far, various studies have been conducted on the relationship between recruiting former bureaucrats board and corporate performance. However, prior studies have amplified persistent questions by showing mixed results from different methods and subjects. Thus, the study sought to observe a clearer relationship between the two major variables through comparative analysis of two different industries. [Methodology] To fulfill the aim of the research, we covered the corporate performances for 20 years from 1998 to 2017. The data were collected from 189 pharmaceutical/medical companies, and another 136 chemical related companies. [Findings] In the medical industry, which is regarded as a unstable institutional environment, the recruitment of former bureaucrats has been observed to have a positive effect on corporate performance, and in the chemical industry, which is considered to be a relatively stable institutional environment, the recruitment of former officials has no significant relationship to corporate performance. [Implications] The study is expected to contribute to explaining the mixed results of the research between recruiting former bureaucrats and corporate performance. In particular, it suggests that the impact of recruiting former bureaucrats on corporate performance may vary depending on the environment of the industry to which the company belongs.

뇌손상 후 운동신경기능 회복 기전 : 기능적 뇌 자기공명영상의 유용성 The Usefulness of fMRI Study

장성호,한봉수,장용민,변우목,김성호,조윤우 한국뇌학회 2001 한국뇌학회지 Vol.1 No.1

목적: 본 연구는 뇌손상환자에서 기능적 뇌 자기공명영상(functional magnetic resonance imaging, fMRI)을 이용한 대뇌운동피질 뇌지도화를 시행하여, 운동신경기능 회복 기전에 대한 기능적 뇌 자기공명영상의 유용성에 대하여 연구하였다. 방법: 신경학적 증상과 병력이 없는 성인 8명과 뇌손상환자 5인을 대상으로 하였다. 뇌손상환자 중 2명은 뇌종양환자이었으며 1명은 뇌출혈, 1명은 뇌경색, 1명은 뇌낭미충증환자이었다. 기능적 자기공명영상은 1.5T MR scanner로 Blood Oxygen Level-Dependent(BOLD) 기법을 적용하였다. 운동과제는 손가락을 1-2Hz의 주기로 쥐었다 펴기를 반복하도록 하였다. 결과: fMRI를 촬영한 결과 정상인 8인, 즉 총 16개의 손중 15개의 손과 환자 5인의 이환되지 않은 손운동시 반대측 일차 운동피질 꼭지(knob)를 중심으로 일차 감각운동피질(SM1)이 활성화되었다. 정상인 중 1인의 한 손에서 반대측 SM1과 동측 일차 운동피질(primary motor cortex, M1) 및 보조운동영역이 동시에 활성화되었다. 뇌손상환자의 이환측 손운동시 환자 1에서는 일차감각피질(primary sensory cortex, S1), 환자 2와 환자 3에서는 양측 SM1, 환자 4와 환자 5에서는 반대측 SM1이 활성화되었다. 환자 1에서는 손상된 M1의 운동신경기능이 S1으로 이전된 것으로 추정되며, 환자 4와 5에서는 손의 운동신경기능이 피질척수로에 의하여 유지되고 있는 것으로 추정되었다. 그러나 환자 2와 환자 3에서 발견된 동측 운동피질 활성화는 본래 정상적으로 존재하던 운동신경 경로인지 뇌손상 후에 발생한 운동신경기능 회복 경로인지 구분할 수는 없었다. 결론: fMRI을 통한 대뇌 운동피질 지도화는 뇌손상 후 병변 주위로의 재조직되는 회복 기전과 피질척수로에 의해 운동신경기능이 유지되고 있음을 입증하는데에 유용할 것으로 생각된다. Objective: This study investigated the usefulness of functional magnetic resonance imaging (fMRI) in the research of motor recovery mechanisms in brain injured patients. Method: Eight control subjects and five brain injured patients were investigated. Out of five patients, two were with brain tumor, one cerebral hemorrhage, one cerebral infarction, and one neurocysticercosis. fMRI was performed using the blood oxygen level-dependent (BOLD) technique at 1.5 TMR scanner with a standard head coil. The motor activation task consisted of finger flexion-extension exercises in 1-2 Hz cycles. Results: fMRI showed activation of the contralateral primary sensori-motor cortex(SM1) by the hand movements of all control subjects and by the unaffected hand movements of all brain-injured patients. Activation of the supplementary motor area(SMA) by one hand movement was also seen in one control subject. Hand movements of the affected hand of the brain injured patients resulted in activation of the contralateral primary sensory cortex(S1) in patient 1, both SM1 in patients 2 and 3, and the contralateral SM1 in patients 4 and 5. Conclusions: It is concluded that fMRI is useful for the research of perilesional reorganization and sparing of the corticospinal tract during the motor recovery mechanisms after brain injury.

작업관련성 수근관증후근 감시체계

정우철,권호장,하미나,노상철,권범선,현정근,이성재,이종민,권정이,김준성,백남종,이호,이경우,이삼규 大韓産業醫學會 2004 대한직업환경의학회지 Vol.16 No.1

목적: 작업관련 근골격계질환은 중요한 직업관련성 질환 중의 하나이고 작업관련 수근관증후군은 이러한 작업관련 근골격계질환 중에서도 많은 부분을 차지한다. 이 연구는 작업관련 수근관증후군의 역학적 특성에 대해 알아보고자 수행되었다. 방법: 본 연구에서는 '수근관증후군 감시체계'를 통해 2000년 6월부터 2003년 2월까지 보고 된 672례의 수근관증후군 사례를 분석하였다. 직업력이 확인된 314명을 대상으로는 직업 및 작업내용에 따라 작업관련성 수근관증후군의 비율이 어떻게 달라지는지를 분석함으로써 수근관증후군 위험요인을 조사하였다. 결과: 직업력이 확인된 314명의 환자 중 작업 관련성이 의심되는 사람은 228명 (72.6%) 이었다. 직업별로는 '단순노무종사자', '농림어업숙련자', '서비스종사자' 등에서 작업관련 수근관증후군의 비율이 여성에서 유의하게 높게 나타났으나 연령, 비만도 지수, 과거병력 등에 따른 차이는 관찰되지 않았다. 주관적 증상 중에 '손을 많이 사용한 후 심해진다'와 '손을 털면 덜해진다'라는 항목을 작업관련성 수근관증후군 환자에서 더 많이 호소하였고 다른 증상은 별다른 차이를 보이지 않았다. 작업관련성 수근관증후군 환자가 비교적 많이 노출되는 작업은 '지나치게 손을 뻗쳐서 하는 일', '손을 불편한자세로 유지하는 일' 등이었다. 결론: 전체 수근관증후군 중 작업관련성이 있다는 비율이 매우 높은 것을 확인할 수 있었다. 수근관증후군 감시체계가 작업관련성 수근관증후군의 여러 특성을 밝히는데 매우 효과적인 것으로 나타났으나 현재까지는 중재 대상을 구체적으로 특정하기에는 한계가 있다. Objectives: Carpal tunnel syndrome (CTS) is one of the most important work related musculo-skeletal diseases in Korea. However, there are few epidemiologic studies on the work-related CTS (WR-CTS). This study aimed to investigate the epidemiologic characteristics of WR-CTS in Korea. Methods: Data obtained from the "CTS Surveillance System". Physician case-reports in the surveillance were used to document patterns of WR-CTS by age, gender, occupation, sign, symptom, working history. Results: Six hundred and seventy-two cases of WR-CTS were ascertained of which 3 14 with complete information on occupational history were analyzed. It has been estimated that as many as 72% of' all CTS cases are work-related. The highest proportion of WR-CTS was observed in 'elementary occupation workers', followed by 'skilled agricultural, forestry and fishery worker'. The distributions of WR-CTS cases were similar with respect to age, obesity, and past medical history. The proportion of WR-CTS was higher in females. There was no significant difference in physical examination findings between WR-CTS and non WR-CTS cases. Repetitive work and the inappropriate hand posture seemed to be the risks for WR-CTS. Conclusion: WR-CTS is a significant public health problem. The CTS surveillance system is quite useful to elucidate the characteristics of WR-CTS, but it remains of limited use in targeting specific industries and occupations for intervention.

순환식 운동프로그램이 대학 운동선수들의 전문체력 및 운동기능 향상에 미치는 영향

노동우(Noh, Dong?Woo),최현호(Choi, Hyun-Ho),이동준(Lee, Dong-Jun) 한국체육과학회 2019 한국체육과학회지 Vol.28 No.3

The purpose of this study was to investigate the effectiveness of elite volleyball players in improving their offense capabilities when combined with circuit training that improves their power and sense of balance that affects volleyball performance. In order to improve the power, agility and endurance of volleyball players, 10 volleyball players at M University were required to perform 13 moves twice a week in the six weeks after the league. As a results on the circuit exercise program, a significant improvement was shown in the extension-and-flexion muscular strength of the left, right pelvic limbs. Also, the back muscle strength and flexibility showed significant, sit-up to muscle endurance showed, side steps to agility were improved, standing height jumps, serve and spike speeds, which recognize the function of volleyball, showed a significant improvement after a circuit training program . These results suggest that the circuit exercise program will help improve the specific fitness and funcitional fitness of volleyball players.