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해바라기, 아몬드, 땅콩의 給與가 생쥐의 增體量 臟器重量 및 血液像에 미치는 影響
朴玉潤,宋瑛敏,金哲浩 진주산업대학교 1990 論文集 Vol.28 No.-
本 試驗은 해바라기, 아몬드 및 땅콩의 種實을 기초사료에 5%수준으로 혼합하여 생쥐에 給與하였을 때 미치는 飼養成績, 臟器重量 및 血液像등을 調査한 結果를 要約하면 다음과 같다. 1. 終了時 體重은 아몬드나 땅콩을 혼합하여 給與하였을 때가 해바라기를 給與하였을 때보다 암수 共히 높았다(p<.05) 2. 內部臟器의 重量에서 肝臟과 腎臟은 해바라기, 아몬드를 給與한 생쥐가 암수 共히 基礎飼料 給與區보다 높았다. 3. RBC, Hb, PCV, MCV 및 MCHC는 해바라기나 아몬드 및 땅콩의 給與에 따른 뚜렷한 影響이 없었고 WBC는 해바라기를 給與하였을 때 다른 處理區보다 높았다. 4. 해바라기를 給與하였을 때 r-GTP와 Total cholesterol含量은 암수 共히 높았으나 BUN含量은 낮았다. This experiment was conducted to investigate the effect of substituting ration with sunflower seed, almond and peanut on the performance, internal organs weight and hemocytological constitution of mouse The cxperimental design was divided into 4 treatments: control, 3 experimental groups added 5% sunflower seed, 5% almond, and 5% peanut to 95% basic diets of mouse The results were summarized as follows: The final body weight of mouse fed the diets substituting ration with almond or peanut seed was significantly(P<0.05) higher compared to those of mouse fed sunflower seed, In the internal organs weight, liver weight or kidney weight of mouse fed the diets sugstituting ration with sunflower or almond seed were higher the those of mouse fed the basic diets There were no difference in RBC, Hb, PCV, MCV, and MCHC ansong mice fed sunflower, almond, and peanut. But WBC in mouse fed sunflower seed was higher the that ao the other treantment groups r-GTP and total cholesterol level of mouse fed sunflower seed were high, but BUN was low
Studies of Structure Activity Relationship of Flavonoids for the Anti-allergic Actions
Ho, Cheong,Ryu, Shi Yong,Oak, Min Ho,Cheon, Seung Hoon,Yoo, Gyurng Soo,Kim, Kyeong Man 전남대학교 약품개발연구소 1998 약품개발연구지 Vol.7 No.1
The structure activity relationship of flavonoids for anti-allergic actions was studied by determining the IC_(50) values for the degranulation. The hexosaminidase release from RBL-2H3 cells (degranulation marker) was employed as an estimate for the anti-allergic actions. Among 22 flavonoid compounds tested, luteolin, apigenin, diosmetin, fisetin, and quercetin were found to be most active with IC_(50) values less than 10 μM.
Oak, Min-Ho,Yi, Eunyoung 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.7
To perform auditory tasks such as sound localization in the space, auditory neurons in the brain must distinguish sub-millisecond temporal differences in signals from two ears. Such high temporal resolution is possible when each neuron in the ascending auditory pathway fires brief action potential at very accurate timing. Various pre- and postsynaptic machineries ensuring such high temporal precision of auditory synaptic transmission have been identified. Of particular, in this review, the role of $K^+$ channels in shortening the duration of synaptic potentials will be discussed. First, the contribution of $K^+$ channels to AP firing of general auditory neurons will be discussed. Then, the focus will be moved to the inner hair cell (IHC)-auditory afferent nerve fiber (ANF) synapses, the first synapses of ascending auditory pathway. Molecular and immunohistological techniques have revealed various $K^+$ channels in the cell bodies and their processes of ANFs. Since the development of patch-clamp recordings from the ANF dendrites in 2002, it became possible to monitor the IHC-ANF synaptic transmission in greater detail. As revealed in brain auditory synapses, several different $K^+$ channels appear to participate in reducing the duration of synaptic potentials at the IHC-ANF synapses. In addition, $K^+$ channels at the ANF dendrites might act as potential targets of efferent feedback from the brain. The hypothesis is that, upon loud sound exposure, efferent neurotransmitters released onto the ANF dendrites activate certain $K^+$ channels and prevent excitotoxicity of ANFs. Therefore, $K^+$ channels of the ANF dendrites might provide potential sites of pharmacological actions to prevent noise-induced hearing loss.
Oak, Min Ho,Cheong, Ho,Kim, Kyeong Man 전남대학교 약품개발연구소 1999 약품개발연구지 Vol.8 No.1
The downstream signaling components of high-affinity IgE receptor (FcεRl) were studied using yeast two-hybrid screening of the cDNA library constructed from RBL-2H3 cells. The cytoplasmic part of the γ-chain but not that of the β-chain was found to interact with pyruvate kinase in the yeast. The in-vitro-translated pyruvate kinase also specifically interacted with the bacterially expressed glutathione-S transferase fusion protein of the cytoplasmic part of the γ-chain. When RBL-2H3 cells were challenged with antigen, the activity of pyruvate kinase gradually decreased, reaching the minimum activity around 5 min after the activation, and then slowly returned to the normal level. The dose-response curve (antigen vs. pyruvate kinase activity) plotted at 5 min after stimulation showed that the pyruvate kinase was dose-dependently inhibited and the maximum inhibition was reached at the concentration of 0.1 ㎍/㎖ of antigen. Direct interaction between FcεRl and pyruvate kinase was also demonstrated by co-immunoprecipitation in RBL-2H3 cells. These data suggest that pyruvate kinase is functionally linked with FcεRl and might exert an important role in controlling cellular functions following the activation of FcεRl.
Angiogenic Squamous Dysplasia as a Biomarker to Predict Progression to Lung Cancer
( Chul Ho Oak ),( Tae Won Jang ),( Maan Hong Jung ),( Bong Kwon Chun ),( Soon Kew Park ) 한국조직공학·재생의학회 2009 조직공학과 재생의학 Vol.6 No.4
Angiogenic squamous dysplasia(ASD) is a term that has been introduced to describe a neoangiogenesis seen in bronchial dysplasias. One of many important angiogenic cytokines are the VEGFs. The VEGFs and their receptors are prime regulators of both physiological and pathological angiogenesis. But it is not clear whether ASD demonstrates the angiogenic switch or not, what is the mechanism of ASD. In this study, Immunohistochemical studies showed an expression of vascular endothelial growth factor in dysplastic bronchial epithelium. VEGF in the dysplastic cells in preneoplastic epithelium was observed in twenty-nine of 31 patients with ASD(93.5%), indicating that angiogenic switch occurred in early preneoplastic lesion including hyperplasia, metaplasia. ASD lesions were not identified in patients with COPD, whereas in 31 of 90 (34.4%) patients with squamous carcinoma, ASD was evident. In addition, the incidence of ASD increased as the hisological grade of preneoplastic lesion advanced, suggesting that the ASD may be a direct precursor lesion(P = 0.03). The author followed up the biologic behavior of dysplasia with respect to the status of ASD. There were significant associations between histological outcome and ASD status during the follow-up period(P = 0.04). These results suggested that ASD be used as biomarkers to predict which dysplasias will progress to squamous cell carcinoma. Macrophages were identified in all(100%) tumor stroma and ASDs. The number of macrophage beneath ASD was significantly higher than that beneath other abnormal epithelium. In addition, the number of macrophages beneath ASD was significantly correlated with the count of microvessels beneath ASD. The results of the present study suggested that Angiogenic squamous dyplasia(ASD) may represent an precursor of lung cancer and may serve as an useful intermediate pathologic biomarker for early detection of lung cancer and chemoprevention. Macrophage beneath ASD may play an important role in early angiogenesis of ASD.
Studies of Structure Activity Relationship of Flavonoids for the Anti-allergic Actions
Cheong, Ho,Ryu, Shi-Yong,Oak, Min-Ho,Cheon, Seung-Hoon,Yoo, Gyurng-Soo,Kim, Kyeong-Man The Pharmaceutical Society of Korea 1998 Archives of Pharmacal Research Vol.21 No.4
The structure activity relationship of flavonoids for anti-allergic actions was studied by determining the $IC_{50}$ values for the degranulation. The hexosaminidase release from RBL-2H3 cells (degranulation marker) was employed as an estimate for the anti-allergic actions. Among 22 flavonoid compounds tested, luteolin, apigenin, diosmetin, fisetin, and quercetin were found to be most active with $IC_{50}$ values less than 10 $\mu\textrm{M}$.
Hong, Oak-Kee,Suh, Sun-Hee,Kwon, Hyuk-Sang,Ko, Seung-Hyun,Choi, Yoon-Hee,Moon, Sung-Dae,Yoo, Soon-Jib,Son, Ho-Young,Park, Kyung-Soo,Lee, In-Kyu,Yoon, Kun-Ho Wiley Subscription Services, Inc., A Wiley Company 2005 Journal of cellular biochemistry Vol.95 No.4
<P>We have proposed that porcine neonatal pancreatic cell clusters (NPCCs) may be a useful alternative source of cells for islet transplantation, and that monolayer cultures might provide an opportunity to manipulate the cells before transplantation. In addition we previously identified 10 genes up-regulated by epidermal growth factor (EGF) in cultured porcine NPCC monolayers. We have now analyzed the intracellular signaling pathways activated by EGF and searched for proteins differentially expressed following EGF treatment of the monolayers, using two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). EGF treatment resulted in phosphorylation of both Erk 1/2 and Akt, as well as increased cell proliferation. Five unknown and 13 previously identified proteins were differentially expressed in response to EGF. EGF treatment increased the expression of several structural proteins of epithelial cells, such as cytokeratin 19 and plakoglobin, whereas vimentin, the intermediate filament protein of mesenchymal cells, and non-muscle myosin alkali chain isoform 1, decreased. Heterogeneous nuclear ribonucleoprotein (hnRNP) A2/B1 factor, which promotes epithelial cell proliferation, and hemoglobin alpha I & II also increased, whereas cyclin A1, immunoglobulin heavy chain, apolipoprotein A1, 5,10-ethylenetetrahydrofolated reductase (5,10-MTHFR), angiotensin-converting enzyme 2 (ACE2), co-lipase II precursor, and NAD<SUP>+</SUP> isocitrate dehydrogenase (NAD<SUP>+</SUP> IDH) alpha chain proteins decreased. Our results show that EGF stimulates proliferation of pancreatic epithelial cells by simultaneously activating the MAPK and PI-3K pathways. HnRNP A2/B1, hemoglobin, cyclin A1, and ACE2 may play roles in the proliferation of epithelial cells in response to EGF. © 2005 Wiley-Liss, Inc.</P>