FK506이 T 림프구 사멸에서 활성산소 생성에 미치는 영향

이호균(Ho Kyun Lee),정상영(Sang Young Chung),최수진나(Soo Jin Na Choi) 대한외과학회 2009 Annals of Surgical Treatment and Research(ASRT) Vol.77 No.5

Purpose: Tacrolimus (FK506) has been widely used as an immunosuppressant in organ transplanted recipients to suppress organ rejection phenomenon. We investigated the role of oxidative stress and heme oxygense-1 by FK506 on human Jurkat T cells. Methods: The cells viability was examined by DAPI stain, enzyme activity of caspase family proteins, and western blotting for Baks, PUMA, iNOS, HO-1. Cells were cultured in the absence or presence of CoPPIX or ZnPPIX and the fluorescence intensity was analyzed using a flow cytometry. Results: Treatment with FK506 increased the generation of reactive oxygen species (ROS), including hydrogen peroxide and superoxide anion, and NO in Jurkat cells in a dose-dependent manner. Immunohistochemistry and Western blot analysis data revealed the hemoxygenase-1 (HO-1) was induced by the addition of FK506 in Jurkat cells. Induction of CoPP, HO-1 inducer, resulted in decreased intracellular H₂O₂ and NO concentrations. Instead ZnPP, an HO-1 competitive inhibitor did it reversely. In addition, ZnPP regulates iNOS protein synthesis by inhibition of HO-1. Conclusion: Increase of HO-1 expression would induce to decrease the intracellular H₂O₂ and NO concentrations. Also, HO-1 would regulate iNOS protein synthesis. Consequently, we can expect the regulation of HO-1 expression with concomitants use of FK506 to suppress organ rejection phenomenon by enhancing apoptosis.

Mycophenolic Acid에 의해 유도된 Jurkat 세포주 세포자멸사에서 Heme Oxygenase-1의 발현이 미치는 영향

이호균(Ho Kyun Lee),최수진나(Soo Jin Na Choi) 대한외과학회 2010 Annals of Surgical Treatment and Research(ASRT) Vol.78 No.6

Purpose: This study demonstrates that pharmacologic induction of heme oygenase-1 (HO-1) along with catalytic activation significantly modulated apoptosis of Jurkat cells induced by mycophenolic acid (MPA). Methods: Cells were cultured with the presence or absence of MPA. Flow cytometric analysis was performed after propidium iodide staining. Western blotting of HO-1, Bcl, and Bax was also performed. Cells were stained 4’-6-Diamidino-2-phenylindole (DAPI) and measured by flow cytometry in the absence or presence of CoPPIX. Results: Treatment of MPA decreased cell viability in a dose- and time-dependent manner. MPA-induced cell death was confirmed as apoptosis characterized by sub G0/G1 phase arrest. Expression of HO-1 assumes a pattern of decline after rising at the initial phase. CoPPIX, HO-1 inducer, significantly inhibited the cisplatin-induced apoptosis. Treatment of MPA resulted in reactive oxygen species (ROS) generation in Jurkat cells. CoPPIX attenuated ROS production in MPA-treated cells. Conclusion: This result suggests that the protective mechanism of HO-1 on MPA-induced cytotoxicity is associated with direct inhibition of ROS generation and mitochondrial permeability transition.

프로젝트관리 시스템을 위한 범위관리 알고리즘 개발

신호균,전승호,김만균 대한설비관리학회 2004 대한설비관리학회지 Vol.9 No.2

This study present the method to cope with the limitations of the existing study that has uniformly been customizing the methodology by only project's complexity. We show how the applied model(ODW) is implemented in PMS using knowledgebase and modelbase which are designed for a scope management algorithm. An ODW model is used for implementation of a WBS identification module and regression model is used for measuring project's complexity. Scope management algorithm is simulated in MS-Excel for estimating its effectiveness and the interrelationship among the knowledgebase, modelbase and database is derived.

主要作物의 微量要素에 關한 硏究 : 第1報 硼素 및 有機物이 배추 生育에 미치는 影響 1. Effects of boron and organic matter on the chinese cabbage

崔元凱,河浩成,鄭鎬權,姜鎬允 진주산업대학교 1969 論文集 Vol.3 No.-

硼素缺乏地帶의 배추圃場에서 硼素의 施用方法 및 施用量을 달리하여 얻은 收量으로 肥效을 追求하고, 또 完熟堆肥의 效果를 調査한 結果를 要約하면 다음과 같다. 1. 硼砂 土壤施用에 依한 배추의 收量은 分散分析結果 1% 水準의 高度의 有意性을 보였으며, 硼砂 10a當 1,500㎏施用의 것이 가장 많은 收量이었고, 그 以上의 硼砂施用에서는 오히려 減少했다. 2. 배추의 硼酸 葉面施用의 差異에 依한 收量差는 土壤施用 때처럼 뚜렷한 差를 볼 수 없었다. 大體로 硼酸 0.25%∼0.5%의 濃度로 葉面撒布한 것이 좋은 結果를 거두었다. 3. 完熟堆肥의 增施에 따라 배추의 收量은 增加되었다. 10a當 1,800㎏ 施用에서 最大値의 收量에 이르고 그 以上의 增施에서는 별다른 收量의 增加를 볼 수 없었다. This experiment was designed to make clear the effects of the application of Boron and organic matter on chinese cabbage growing in Boron dificient soil. The results obtained were as follows. 1. The application of Borax in increased remarkably the yield of the chinese cabbage. The yield was increased by the application of Borax in the soil. It reached maximum amounts at 1,500g per 10a, but decreased above 1,500g per 10a. 2. The various concentration of Boron on the leaf spray was no more effective than the application of Boron in soil. The adequate concentration of Boric Acid on the leaf spray was from 0.25 percent solution to 0.5 percent solution. 3. The yield of the chinese cabbage was increased by the application of the compeletely decayed compost. It reached maximum amounts at the application if 1,800㎏ per 10a, but there was no effects above 1,800㎏ per 10a.

조구등(釣鉤藤)의 4-VO로 유발한 흰쥐뇌허혈에 대한 신경방어효과

李仁煥,林康鉉,李鍾錫,石庚浩,安德均,朴虎君,김頀哲 대한본초학회 1999 大韓本草學會誌 Vol.14 No.2

Uncariae Ramulus et Uncus(UR, ???) has sweet in flavour and slightly cold in property, acting on the liver and pericardium channels. This drug was described in a medical classic as having the ability to remove "heat", check hyperfunction of the liver and relieve dizziness, tremors, and convulsion, and subdue "endogenous wind". So this study was planned to check the neuroprotective effect of UR on the global ischemia induced by 4-vessel occlusion in Wistar rats. and UR extract was lyophilized after extraction with 70% methanol. We induced 4-vessel occlusion for 10 minutes and reperfused again. The number of CA1 pyramidal neurons were counted after 7 days of reperfusion under the cresyl violet staining. In 4-VO ischemia model, UR showed significantly neuroprotective effects(1,000 and 500 ㎎/㎏ of UR extracts, p<0.05) compared with control group. Each neuroprotective ratio was about 23.0%, 19.0% respectively. Consequently, Uncariae Ramulus et Uncus has neuroprotective effects on the global ischermia induced by 4-vessel occlusion in Wistar rats. So we expect that Uncariae Ramulus et Uncus can be used as a drug for neurodegenerative disease.

降伏線 解析에 의한 開口部를 둔 슬래브에 관한 硏究

진호균,김종수 釜山工業大學校 1995 論文集 Vol.37 No.-

There are some cases where the structure of slab needs to have openings according to the conditions of its uses. The research done in this paper deals with the ultimate load condition to which the slabs with openings are subjected on the basis of Moondra's, Rangaswami's and Ockleston's studies. Comparisons are the experimental results of three slabs with openings different in shapes and sizes in the simply-supported circular slabs subjected to uniformly distributed load. As a result, the actural load to which the slabs with openings are subjected is 1.36 to 1.92 times larger than the theoretical ultimate load. As the size of opening increases, the ultimate load that the capacity of slabs can carry decreases.

精神治療와 修道 : 精神分析과 禪을 中心으로

尹虎均 聖心女子大學校 1991 論文集 Vol.23 No.-

개인의 삶의 태도는 그가 집착하는 대상에 따라 자기중심적일 수도 있고, 타인중심적일 수도 있고, 규범중심적일 수도 있다. 그러나 이러한 집착에서 벗어난 사람은 초월적일 수 있다. 정신치료(정신분석)나 수도(선)는 다 같이 자신의 집착으로부터의 해방을 목표로 한다. 그러나 정신분석에서는 주요한 무의식적 감정이나 욕구들로부터 자유로워지는 것을 추구하고, 선에서는 일체의 의식적 내지 무의식적인 집착들로부터 해탈하는 것을 추구한다. 전신분석에서의 무의식적 욕구나 감정으로부터의 해방은 무의식에 대한 통찰을 통한 자아의 강화를 뜻하며, 선에서의 일체의 의식적 무의식적 집착으로부터의 해탈은 참된 자기에 대한 깨달음의 산물로서의 자기의 초월인 동시에 자기의 실현이다. 정신분석에서의 통찰이나 선에서의 깨달음은 둘다 자기의 내부에 대한 의식의 확장을 가져온다. 그러나 정신분석에서 내담자가 통찰하는 것은 자기의 억압된 성과 공격성(사랑과 미움)이지만, 선에서 수도자가 깨닫는 것은 자기의 참 마음이다. 또한 정신분석과 선은 그 方法의 측면에서 몇가지 뚜렷한 차이를 보인다. 첫째로 정신분석에서는 치료자는 내담자에게 전이의 대상이 되어주고 전이를 해석해주는 사람으로서 그 존재가 필수적이다. 그러나 선에서는 수도자 스스로가 자기에게 주어진 화두를 참구하는 데 집중하는 것이 핵심을 이룬다. 둘째로 정신분석에서는 내담자는 경험을 하는 자아와 관찰을 하는 자아로서의 자아의 치료적 분열이 필수적이나, 선에서는 온 심신이 화두와 한 덩어리가 되어야 한다. 세째, 정신분석에서는 理性, 즉 언어화·개념화와 논리적 사고의 전개를 중시하지만, 선에서는 개념과 생각을 떠나 直觀을 요구한다. 그리고 정신분석에서는 집착을 찾아내어 이를 통제하는 것에 역점을 두나, 선에서는 오로지 화두를 깨치므로써 자기으 참된 마음을 깨닫는 일에만 신경을 쓴다. 정신치료와 수도는 자기자신과 세계를 보다 합리적으로 그리고 이성적으로 즉 객관적으로 이해하는 능력을 향상시키고, 때로는 자신을 무조건적으로 받아들이고 불안없이 있는 그대로 이해하는 공감적인 이해의 능력을 향상시킨다. 반면 선은 자신과 세계를 아무런 선입견이나 개인적인 욕망이나 감정을 투사함이 없이 맑은 마음으로 볼 수 있는 직관 능력의 향상을 초래한다.

養生에 對한 小考

陳鎬均 釜山工業大學校 1969 論文集 Vol.9 No.-

分配傳達行列 및 轉播行列을 이용한 Vierendeel Rahmen의 解法에 關한 硏究

陳鎬均 釜産工業大學校 1984 論文集 Vol.25 No.-

시변스위칭함수를 이용한 슬라이딩 모드 제어기의 도달기간 제거에 관한 연구

안호균,곽군평 國立 昌原大學校 産業技術硏究所 1998 産技硏論文集 Vol.12 No.-

This paper presents the design of sliding mode Controller for removing reaching phase using time varying switching functions. For removing reaching phase in VSCS (Variable Structure Control Systems), a time varying switching surface is proposed to achieve the sliding mode during the entire control process. Numerical example is presented to demonstrate the effects of this approach.