Eriodictyol Protects Endothelial Cells against Oxidative Stress-Induced Cell Death through Modulating ERK/Nrf2/ARE-Dependent Heme Oxygenase-1 Expression

Lee, Seung Eun,Yang, Hana,Son, Gun Woo,Park, Hye Rim,Park, Cheung-Seog,Jin, Young-Ho,Park, Yong Seek MDPI 2015 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.16 No.7

<P>The pathophysiology of cardiovascular diseases is complex and may involve oxidative stress-related pathways. Eriodictyol is a flavonoid present in citrus fruits that demonstrates anti-inflammatory, anti-cancer, neurotrophic, and antioxidant effects in a range of pathophysiological conditions including vascular diseases. Because oxidative stress plays a key role in the pathogenesis of cardiovascular disease, the present study was designed to verify whether eriodictyol has therapeutic potential. Upregulation of heme oxygenase-1 (HO-1), a phase II detoxifying enzyme, in endothelial cells is considered to be helpful in cardiovascular disease. In this study, human umbilical vein endothelial cells (HUVECs) treated with eriodictyol showed the upregulation of HO-1 through extracellular-regulated kinase (ERK)/nuclear factor erythroid 2-related factor 2 (Nrf2)/antioxidant response element (ARE) signaling pathways. Further, eriodictyol treatment provided protection against hydrogen peroxide-provoked cell death. This protective effect was eliminated by treatment with a specific inhibitor of HO-1 and RNA interference-mediated knockdown of HO-1 expression. These data demonstrate that eriodictyol induces ERK/Nrf2/ARE-mediated HO-1 upregulation in human endothelial cells, which is directly associated with its vascular protection against oxidative stress-related endothelial injury, and propose that targeting the upregulation of HO-1 is a promising approach for therapeutic intervention in cardiovascular disease.</P>

AISI D2강 위에 다층으로 증착된 WC-Ti_(1-X)Al_(X)N 코팅의 국부부식 거동에 관한 연구

안승호,이정호,김호건,김정구,한전건 대한금속재료학회 2003 대한금속·재료학회지 Vol.41 No.9

Multilayered WC-Ti_(1-x)Al_(x)N coatings are characterized by periodically repeated structures of lamellae of WC-Ti/WC-Ti_(1-x)Al_(x)N. WC-Ti_(1-x)Al_(x)N coatings of changing Al concentration were deposited on AISI D2 steel by high-ionization sputtered plasma vapor deposition (PVD). The Al concentration could be controlled by using evaporation source for Al and fixing the evaporating rate of other metals. Four kinds of WC-Ti_(1-x)Al_(x)N coatings were prepared: WC-Ti_(0.6)Al_(0.4)N, WC-Ti_(0.53)Al_(0.47)N, WC-Ti_(0.5)Al_(0.5)N and WC-Ti_(0.43)Al_(0.57)N. The corrosion behavior of WC-Ti_(1-x)Al_(x)N coatings was investigated using electrochemical techniques in deaerated 3.5% NaCl electrolyte (galvanic corrosion test, potentiodynamic polarization test, electrochemical impedance spectroscopy and Mott-Schottky method) and surface analyses (GDOES, SEM and EDS). Particular attention was paid to the effect of growth defects on the coating properties related to the corrosion behavior. The results of the coating and substrate galvanic corrosion test showed low galvanic current densities. In the potentiodynamic polarization test and EIS measurement, the corrosion current density of WC-Ti_(0.5)Al_(0.5)N was lower than others and presented higher R_(ct) values than others after 240 h immersion time. This could be attributed to the formation of a Ti oxide, which promotes partial closing of the defects. Capacitance values obtained from EIS as a function of different potentials (C^(-2) vs E) showed that the oxide layer exhibited an n-type semiconductor behavior.

Crotonaldehyde-exposed macrophages induce heme oxygenase-1 expression as an adaptive mechanism

Seung Eun Lee,Hana Yang,Gun Woo Son,Hye Rim Park,Young-Ho Jin,Cheung-Seog Park,Yong Seek Park 대한독성 유전단백체 학회 2015 Molecular & cellular toxicology Vol.11 No.2

Cigarette smoke represents one of the most significant risk factors for the progression of vascular disease. Exposure to cigarette smoke induces oxidative stress in the vascular system and results in vascular dysfunction. Crotonaldehyde, a highly reactive α,β-unsaturated aldehyde, is a foremost compound of cigarette smoke and a product of endogenous lipid peroxidation. Heme oxygenase-1 (HO-1) expression plays an essential role in cellular defense against environmental insults and represents an adaptive response. Here, we showed the effects of crotonaldehyde on the induction of HO-1 expression in RAW 264.7 macrophages. Crotonaldehyde treatment resulted in significantly increased phosphorylation of p38 mitogenactivated protein kinase (MAPK) and nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2). Furthermore, treatment with zinc protoporphyrin (ZnPP; the specific HO-1 inhibitor) markedly augmented the death of crotonaldehyde-treated macrophages. In summary, these results highlight the role of the HO-1 upregulation through p38 MAPK-Nrf2 activation in the adaptive response to crotonaldehyde in macrophages.

편마비 환자의 정상측과 마비측의 비복근 운동점에서의 근육 두께 차이

김동건,도현경,김현동,황지선,정규영,정호중 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.1

Objective : To investigate any difference of muscle thickness in the motor points of the gastrocnemius between the paretic and non-paretic legs in hemiplegics. Method : Ultrasonography was used to assess in vivo the gastrocnemius muscle thickness at the motor points in the paretic and non-paretic legs of twenty-four patients with stroke. Results : Muscle thickness at the motor points were reduced in the paretic legs compared to the non-paretic legs (p<0.05). There was no correlation between muscle thickness and duration of hemiplegia, site of hemiplegia. However, it did not take regular pattern of the muscular atrophy in the paretic legs. Conclusion : The gastrocnemius muscle of the paretic leg had smaller thickness compared with the non-paretic leg. The present results indicate that paresis in hemiplegics may affect the accuracy of intramuscular injection of botulinum toxin A. Therefore, we recommend ultrasonography for visually controlled, anatomically precise injection of botulinum toxins.

중남미 지역공동체와의 경제협력을 통한 한국 무역의 발전방향에 관한 연구

이호건 청주대학교 경영경제연구소 2016 경상논총 Vol.8 No.2

중남미 지역은 1820년경에 스페인과 포르투갈로부터 독립한 국가들로 라틴계의 이민족에 의해 형성된 국가들이다. 따라서 아시아처럼 역사가 있는 국가의 형태보다는 동질적 문화적 배경을 갖고 언제든 정치‧경제적 이해관계에 따라 변화될 지역주의적 개념이 강하다. 2000년대 초반까지는 역내 경제공동체를 구성하여 통합해 나가는 모습을 보였으나 대외적 파급력은 미약하였다. 최근들어 중남미 국가들은 정치적으로 통합된 모습으로 33개 모든 중남미 국가들이 참여하여 CELAC을 출범시켰으며 기존의 폐쇄적인 MERCOSUR에 대응하는 개방형 경제연합체로서 Pacific Alliance가 출범함으로서 중남미 시장 진입의 관문 역할을 하고 있다. 한편 중남미는 풍부한 천연자원을 보유하고 농수축산물을 생산하는 지역으로 한국 경제 발전을 위해서는 중장기적으로 반드시 확보해야 하는 시장이라 할 수 있다. 현재 한국의 중남미 시장에서의 무역 비중은 4.9% 정도이나 2006년에서 2015년까지 한국의 전체 무역수지에서 차지하는 흑자의 비중은 최소 13.27%에서 최고 87.6%에 달한다. 이에 본 연구에서는 이와 같은 중남미 시장에 진입하기 위하여 중남미 국가들의 지역주의적 특성과 경제공동체를 이해하고 무역흑자국으로서 마찰을 최소화하며 현지에 진출하기 위한 방안을 연구하였다.

태권도 옆차기 동작의 우수자와 비우수자간의 운동학적 비교 분석

이호진,한재희,김륜건,홍예은,우상연 순천향대학교 기초과학연구소 2022 순천향자연과학연구 논문집 Vol.28 No.1,2

The purposes of this study were to analyze the kinematic variations between skilled and unskilled individuals and obtain the data needed to improve effective side kick skill in Teakwondo. We used a video analysis program to collect performance time, changes in movement of the center of the body, angle, and angular velocity. The results were as follows : 1) in unskilled cases, side kick motion occurred for more time than in unskilled cases, 2) to make a stronger kick, the skilled group increased the joint speed of the legs and unskilled athletes have smaller angles than experienced athletes. As a result, the total performance time of side kicks was shorter for skilled people and was consistent with other previous studies. The angle of the knee joint and hip joint had a great influence on the side kick.The range of motion of the hip and knee joints should be increased so that the desired hitting point can be quickly reached.

중부지역 옥수수 파종기에 따른 생육단계 및 수량성 변화

서종호, 문중경, 정건호, 서민정, 구자환, 박장환, 배환희, 신성휴, 권영업 忠北大學校 農業科學硏究所 2014 農業科學硏究 Vol.30 No.2

Maize hybrids were planted on April 1, April 25, May 20 and June 15 in 2012, 2013 at the experimental paddy and upland fields in Suwon city , respectively to know changes of growth stage and yield according to different planting time. Maize planting on April 1 was not recommended because total growing days at planting on April 1 was not different with that at planting on April 25 due to many days from planting to emergence over 20 days at planting on April 1. Total growing days were not much different among three planting time after April 25 because days from silking to maturity was lengthened though days from planting to silking was shortened at later planting time. Stalk weight was decreased as planting time was delayed, particularly at planting times after May 20 at paddy field by excess water injury during early growth stage. But, grain yield was not low as in May and June. It is needed to introduce early maturity maize variety as Yanganok at late planting for higher grain yield and enough time for soil preparation for next winter crops

도립진자의 디지틀 제어

윤호성,이건복 崇實大學校 生産技術硏究所 1992 論文集 Vol.22 No.-

The inverted pendulum problem, which keeps the pendulum in the upright position, is a typical problem of unstable systems and has been studied by many researchers. In this work a digital controller is designed not only to stabilize the pendulum in the upright position but also to control the cart location at a given point. Controller design is based on a linear quadratic(LQ) optimal control theory. The controller is designed to achieve good performance characteristics regardless of uncertainties due to modeling errors and disturbance using frequency response shaping of overall system. The system is proved to work properly through simulation and experimentation.

硏削條件에 따른 硏削表面 거칠기와 硏削低抗과의 관계에 대하여

金建鎬 군산대학교 1985 群山水産專門大學 硏究報告 Vol.19 No.3

This study was analyzed by the geometrical theory and experiments on the correlation between the surface roughness and the resistance force in the grinding condition. The results are as follows. 1.The increaser grinding wheel speed, the better effection on the surface roughness and the resistance force . 2.The increaser workpiece speed, grinding depth and feed, the worse effection on the surface roughness and the resistance force.

CE-marking 제도와 우리나라 기업의 대응방안

차근호 한국항공대학교 경영연구소 1998 경영연구 Vol.5 No.1

The CE Marking offers great advantages to trade and industry in the Korea The new European legislation has created a uniform market within Europe. Up to now, Korean manufacturers and exporters have had to deal with various sets of national legislation within Europe. Thanks to the introduction of the CE Marking, the trade constraints between the Member States of the European Economic Area(EEA) have disappeared. The CE Marking forms, as it were, the "trade passport" for products within the EEA. This paper aims to showing the development-process of the CE marking and to suggesting the responsive strategies preparing for the CE marking. Companies in the Korea that take swift action according to the suggestions are ensured of a better competitive position in respect of companies in other continents.