http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Franklin Hinosa Mancia,주윤하,임기병,김정선,남상용,황윤정 한국자원식물학회 2017 한국자원식물학회지 Vol.30 No.6
Dual-color fluorescence in situ hybridization karyotype analysis was created using repetitive sequences including two types of rDNA repeats (45S and 5S rDNAs) and Arabidopsis-type telomere sequence repeats. The somatic metaphase cells of Carthamus tinctorius were observed as diploids (2n=2x=24). A symmetrical or slightly asymmetrical karyotype with seven pairs of metacentric and five pairs of submetacentric chromosomes was observed. The lengths of the somatic metaphase chromosomes ranged from 4.18 to 6.53 ㎛, with a total length of 60.71 ㎛. One locus of 45S rDNA was located on the pericentromeric regions of three pairs of chromosomes and the other pair was situated on the terminal regions of the short arms of a single pair of chromosomes. One locus of 5S rDNA was detected on the interstitial regions of the short arms of two pairs of chromosomes. Arabidopsis-type telomeric repeats were detected on the terminal regions of all pairs of chromosomes. Co-localization of loci between telomeric repeats and 45S rDNA was observed in a single pair of chromosomes. The results provide additional information for the existing physical mapping project of C. tinctorius and will also serve as a benchmark to a more intricate cytogenetic investigation of C. tinctorius and its related species.
FISH Karyotype Analysis of Fagopyrum tartaricum Gaerth (Tartary buckwheat) using Repetitive DNAs
Yoon-Ha Ju,Franklin Hinosa Mancia,Seyong Yoon,Heekyung Park,Soeun Yoon,Jiin Park,Sunmi Joung,Su Jeong Kim,Yul Ho Kim,Hwang Bae Sohn,Ki-Byung Lim,Hyun Hee Kim,Yoon-Jung Hwang 한국원예학회 2016 한국원예학회 학술발표요지 Vol.2016 No.10
Cytogenetic Analysis of Bienertia sinuspersici Akhani as the First Step in Genome Sequencing
Samantha Serafin Sevilleno,Yoon Ha Ju,Jung Sun Kim,Franklin Hinosa Mancia,Eun Ju Byeon,Raisa Aone Cabahug,Yoon‑Jung Hwang 한국유전학회 2020 Genes & Genomics Vol.42 No.3
Background C4 plants are efficient in suppressing photorespiration and enhancing carbon gain as compared to C3 plants. Bienertia sinuspersici Akhani is one of the few species in the family Amaranthaceae that can perform C4 photosynthesis within individual chlorenchyma cells, without the conventional Kranz anatomy in its leaf. This plant is salt-tolerant and is well-adapted to thrive in hot and humid climates. To date, there have been no reported cytogenetic analyses yet on this species. Objective This study aims to provide a cytogenetic analysis of B. sinuspersici as the first step in genome sequencing. Methods Fluorescence in situ hybridization (FISH) karyotype analysis was conducted using the metaphase chromosomes of B. sinuspersici probed with 5S and 45S rDNA and Arabidopsis-type telomeric repeats. Results Results of the cytogenetic analysis confirmed that B. sinuspersici has 2n = 2x = 18 consisting of nine pairs of metacentric chromosomes. Two loci of 45S rDNA were found on the distal regions of the short arm of chromosome 7. Nine loci of 5S rDNA were found in the pericentromeric regions of chromosomes 1, 3, 4, 6, and 8, which also colocalized with Arabidopsis-type telomeric repeats; while four loci in the interstitial regions of chromosome 5 and 8 can be observed. The single locus of 5S rDNA that was found in chromosome 8 appears to be hemizygous. Conclusion The FISH karyotype analysis, based on the combination of rDNAs, telomeric tandem repeat markers and C0t DNA chromosome landmarks, allowed efficient chromosome identification and provided useful information in characterizing the genome of B. sinuspersici.