Effect of Plant Growth Regulators on Clonal Production through Basal Stem Explant Cultures of a Phalaenopsis Hybrid

Jo,Man-Hyun,Ham,In-ki,Park,Sang-kyu,Seo,Gwan-Seok,Han,Gyu-Heung,Woo,In-Shik 한국자원식물학회 2003 Plant Resources Vol.6 No.2

This study was conducted to develop the clonal propagation technique through in vitro culture using basal stem explants in Phalaenopsis hybrid grown in vitro. The highest frequency of protocorm-like body (PLB) formation was obtained when basal stem explants were cultured on VW medium containing 30g/L sucrose, 500 mg/L activated charcoal, 150 ml/L coconut water, 1 mg/L NAA, 5 mg/L 2iP and 2.5g/L gel rite. PLBs transferred to Hyponex medium were regenerated to plantlets. Plantlets transferred to plastic pots containing spagnum moss were developed and successfully acclimatized under greenhouse. The flower was bloomingly opened in plants regenerated from basal stem explants. The flower was not different from both mother plant and plant induced through clonal propagation of Phalaenopsis hybrid.

Clonal Propagation through Leaf Sheath Culture of Phalaenopsis

Jo,Man-Hyun,Ham,In-Ki,Lee,Mi-Ae,Han,Gyu-Heung,Woo,In-Shik 한국자원식물학회 2002 Plant Resources Vol.5 No.3

This study was conducted to develop the clonal propagation technique through in vitro culture using by leaf sheath explants of Phalaenopsis grown in vitro. The highest frequency of protocorm-like body (PLB) formation was obtained when explants of leaf sheath were cultured on VW medium containing 30g/L sucrose, 500 mg/L activated charcoal, 150 mVL coconut water, 1 mg/L NAA, 1 mg/L 2ip and 2.5 g/L gelrite. The PLB formation rate of VW medium was highest followed by modified Hyponex medium, and lowest in MS medium. Plantlets induced from PLBs transferred to plastic pots including spagnum moss were well developed.

Effect of In-row Plant Spacing on Growth and Yield of Korean Native Allium wakegi Araki

Jo,Man-Hyun,Ham,In-Ki,Park,Sang-Kyu,Seo,Gwan-Seok,Han,Gyu-Heung,Woo,In-Shik 한국자원식물학회 2003 Plant Resources Vol.6 No.2

Allium wakegi Araki was grown at plant spacings of 5, 10, 15, and 20 cm to determine the effect of planting density on the growth and yield. Allium wakegi Araki plants grown at the 5 cm plant spacing had the lowest bulb diameter and bulb weight, while plants at the lowest density (20 cm spacing) had the highest bulb diameter, bulb number, bulb weight and fresh weight. In general, plants grown at narrower spacings produced significantly smaller bulb diameter and bulb weight, but resulted in the highest yields and plants per hectare and lower fresh weights per plant.

저장용액의 온도에 따른 치주인대세포의 생존율

조재현,김성오,최형준,이제호,손흥규,최병재 대한소아치과학회 2007 大韓小兒齒科學會誌 Vol.34 No.1

외상성 손상 후 치아 탈구 시 치아를 저장하는 저장용액의 종류와 온도가 치주인대세포의 생존율에 어떠한 영향을 미치는지 연구하기 위하여 치주인대세포를 10% fetus bovine serum(FBD) 함유 α-minimal essential medium(α-MEM)에서 37℃ 5% CO_(2) 공기 혼합 배양기에서 배양하고 4.25, 37℃의 Hank's balanced sa1t solution(HBSS)과 α-MEM, 우유(S회사, P회사), tap water에 저장하고 60분이 지난 후 각 군에 대해 치주인대세포의 생존율을 측정하기 위하여 MTT assay를 시행하여 다음과 같은 결과를 얻었다. 1. 4℃ 저장용액의 치주인대세포의 생존율은 α-MEM과 P회사우유에서 가장 높았고 HBSS, S회사우유, tap water 순으로 낮았다. 2. 25℃ 저장용액의 치주인대세포의 생존율은 α-MEM에서 가장 높았고 P회사우유, HBSS, S회사우유, tap water 순으로 낮았다. 3. 37℃ 저장용액의 치주인대세포의 생존율은 α-MEM과 P회사우유, HDSS, S회사우유에서 높았고 tap water에서 가장 낮았다. 4. α-MEM의 치주인대세포의 생존율은 4℃에서 가장 높았고 25℃와 37℃ 순으로 낮았으며 HBSS에서는 4℃에서 높았고 25℃와 37℃에서 낮았다. 5. S회사와 P회사우유에서 치주인대세포의 생존율은 4℃와 25℃에서 높았고 37℃에서 낮았다. 이상의 결과를 종합해볼 때 외상으로 인해 치아가 탈구되었을 때 탈구된 치아의 저장용액으로 HBSS 용액이 추천되고 있으나 이 연구에서 4℃와 25℃ 우유에서 치주인대세포의 생존율이 높았으므로 사고 현장에서 쉽게 구할 수 있고 치주인대세포의 생활력 보존에도 유리한 낮은 온도의 우유에 탈구된 치아를 보존하는 방법도 좋을 것으로 생각된다. To compare the survival rate of periodontal ligament cells preserved in storage media with good availability at the time of an avulsion injury, periodontal ligament cells were incubated in α-MEM culture medium containing 10% FBS in condition of 37℃, 5% CO_(2). These cells were then cultured in HBSS, α-MEM, milk(S. co., P. co.) and tap water at the temperature of 4, 25, 37℃ each in 60 min. The groups were measured by MTT assay. The results were as follows : 1. Among the storage media at 4℃, α-MEM and P-milk had the highest preserving ability of periodontal ligament cells, while that of HBSS, S-milk and tap was low in order. 2. Among the storage media at 25℃, α-MEM had the highest preserving ability of periodontal ligament cells, while that of P-milk, HBSS, S-milk, tap water was low in order. 3. Among the storage media at 37℃, the preserving ability of periodontal ligament cells was very high in α-MEM, P-milk, HBSS and S-milk. it' s lowest in tap water. 4. The preserving ability of periodontal ligament cells in α-MEM was high at 4℃ and it’s low in order of 25℃, 37℃, but in HBSS was high at 4℃ and it's low at 25℃, 37℃. 5. The preserving ability of periodontal ligament cells in S-milk and P-milk was high at 4℃, 25℃ and it’s low at 37℃. In conclusion, HBSS is the storage medium of choice in an avulsion, but in this study it is preferable to choose milk at 4℃ for tooth since it is easy to get and affect cell viability.

Junger and Feit의 실린더운동방정식과 새로이 유도된 방정식에서 실린더좌표방향의 진폭항 비교 연구

조형국 東西大學校 1997 동서논문집 Vol.3 No.1

실린더 형태의 관은 산업체나 일반가정에서 자주 사용되어 진다. 이러한 실린더형태의 관들은 유체나 기체를 운반하는데 쓰이며, 운반과정에서 소음이 발생한다. 이러한 소음들의 해석은 우선 실린더운동방정식을 유도하여야 한다. 음향학 분야에서 여러 가지 다른 형태의 실린더운동방정식이 소개되었다. 예로써, Junger and Feit는 실린더의 벽이 매우 얇고, 무한히 긴 실린더를 가정하에서 실린더운동방정식을 유도하였다. 본 논문에서는 참고문헌[5]에서 유도된 실린더운동방정식과 Junger and Feit의 실린더운동방정식에서 실린더 좌표 방향으로 향하는 변위의 항을 비교하고, 임의의 실린더 크기를 가정하여 항의 차이를 그래프로 보였다. Cylinderical shells are usually used for transforting gas or fluid in the industries or houses. A Cylinderical shell equation turns out to be important in noise generating and noise controlling. The analysis of the noise need to the cylinder motion equation. Several different types of cylinderical shell equation has been introduced in the field of acoustics. As are example, Junger and Feit derived the cylinderical equation on assumption that the cylinder is thin and infinite long. Two different sets of displacements in cylinderical coodinates, are produced in the Junger and Feit's equation and in the new cylinderical equation, respectively. These two sets of solutions will be compared in this paper. The difference in the two sets of solution will be show in the several graphes.

그레이브스병에서 방사성 요오드 치료에 따른 갑상선 자극 호르몬 수용체 결합억제 면역글로불린(Thyrotropin Binding Inhibitory Immunoglobulin, TBII)의 변화

조영석,권기현,이준철,나소영,이효진,홍우정,이유선,김군순,송민호,김영건,노흥규 충남대학교 의학연구소 2003 충남의대잡지 Vol.30 No.2

Prediction of thrapeutic response to radioiodine in Graves' disease is poorly understood. Although thyrotropin binding inhibitor immunoglobulin(TBII) level is a strong index for relapse after antithyroid drug medication, conflicting results are reported regarding its prognostic significance in Graves' disease treated with RAI. This study is dengned to evaluate possible relationship between post-treatment hypothyroidism and TBII in Graves' disease treated with RAI. Fourty two patient with Graves' disease after radioiodine treatment were studied retrospectively. The subject were divided into hypothyroid group and euthyroid or hyperthyroid group. We evaluated the association of hypothyroidism and TBII with radioiodine treatment dose. The mean age of hypothyroid group was 48±11 years and euthyroid or hyperthyroid group was 47±12 years. There was no difference in two groups. And there was no significant difference in post- treatment TBII between two groups(49.9±28.5%, 29.9±14.3%, p-value >0.05). The treatment-dose had no influence on post-treatment thyroid state. Euthyroid or hyperthyroid group was done with 13.6±6.9mCi and hypothyroid group was 17.0±10.4mCi(p-value > 0.05). TBII had no prognostic significance on long-term hypothyrodism in Graves' disease treated with radioiodine. And, treatment-dose had no influence on post-treatment thyroid state.

새로이 유도된 실린더에 의한 실린더의 진폭 비에 대한 비교연구

조형국,이종년 東西大學校 1998 동서논문집 Vol.4 No.-

실린더로 구성된 시스템에서 외부의 자극으로 인하여 일어나는 소음의 해석은 중요하다. 실린더에서 일어나는 소음의 해석을 위해서 실린더 운동방정식이 필요하며, 지금까지 많은 학자들이 실린더 운동방정식을 유도하였다. 이 유도된 운동방정식은 실제 응용에서 오차가 발생하며, 그 오차를 없애기 위해 새로운 실린더 운동방정식이 도입되었다. 새로운 실린더 운동방정식으로 자유 운동시 실린더에서 음의 발생에 큰 원인이 되는 redial방향의 변위 진폭과 tangential 그리고 axial 방향에 대한 변위 진폭 비를 관찰하여 보았다. The Noise generated in cylinders by an external excitation has attracting concerns of many researchers. The analysis of the noise in cylinders requires governing of its motion, and hence many researchers have studied on derivation of the equations in many ways. Among them, the equation derived by Junger and Feit result critical errors in wave propagation when they are applied to some practial examples. In order to reduce the errors. the new cylinder equations are introduced by Jo[7]. Amplitude ratios of radial to tangential and longitudinal directions and directions of displacements are closely observed after are applied to a cylinder excited by an external impact.

農民들의 農産物流通情報 活用實態分析

趙興洙 안성산업대학교 1985 論文集 Vol.17 No.-

Though information on current Market price and condition is very important, it is by no means the whole area of useful and need market information. Data are need on current and prospective production levels, for production levels indicate what future market supplies will be. Usually, the earlier that production information is available, the more useful it is. The study was carried out to understand farmers, practical use on the agricultural Market Information, by means of the situation survey of four hundred-fourty farm household of Korea and by review of seveal public statistic reports. In order to raise the realities of practical use in agricultural market information and to promote efficiency of agricultural marketing, It was concluded that it should supplement marketing education and should have an all-out agricultural market information system. It has to manage cooperatively, by farmers as well as by the integrated support of government and public organizations related to agricultural marketing development.

125KHz를 동작 주파수로 하는 RFID 시스템 개발

조형국 東西大學校 2002 동서논문집 Vol.8 No.-

RFID는 Radio Frequency Identification의 약자로서 라디오 주파수를 이용하여 어떤 대상물을 인지하는 방식을 의미한다. 라디오 주파수의 이용이라 함은 대상물에 접촉을 하지 아니하고 대상물이 가지고 있는 고유의 정보를 수신 측에 전달하는 것이다. 일반적으로 데이터나 에너지의 전달에 사용되는 주파수는 여러 가지가 있다. 예로서, 125KHz, 13.56MHz, 5.6GHz 등이 있다. 원활한 에너지의 전송을 위해서는 125KHz를 사용한다. 5.6GHz는 일반적으로 액티브 형태로 사용한다. 본 논문에서는 125KHz를 사용하는 RFID 시스템을 개발한다. 이유는 에너지의 전달을 보다 쉽게 하기 위해서이다. 트랜스폰드는 microchip 사에서 개발한 패시브 타입인 MCRF 250을 사용하였다. 우선 MCRF 250의 동작을 위한 시스템의 설계를 위한 기본 개념을 연구하고, 시스템을 설계하였다. 그리고 안테나의 설계 방법 등을 제시하였다.

자유파수를 이용한 새로운 실린더 운동방정식과 Junger and Feit의 실린더 운동방정식의 비교연구

조형국,김정국 東西大學校 1996 동서논문집 Vol.2 No.1

실린더 운동방정식은 실린더 내에서 발생되는 소음 해석에매우 중요하다. 그러므로, 지금까지 많은 학자들에 의해 실린더 운동방정식을 유도되었다[1],[2]. 본 논문에서는, 참고문헌[5]에서, 새로 제안된 실린더 운동방정식과 Junger and Feit의 실린더 운동방정식을 보이고 가정해에 의한 선형 방정식을 제안하였다. 실린더가 자유 운동을 할 때, 두 실린더 운동방정식의 선형 방정식으로 자유파수를 구하였고, 결과를 그림으로 서로 비교하였다. 이 결과, 새로 유도된 실린더 운동방정식의 타당함을 보였다. 새로 제안된 실린더 운동 방정식을 이용하면, 산업현장에서, 실린더 내의 고체파에 대한 보다 정확한 분석을 할 수 있을 것으로 사료된다. The Cylinderical Shell Equation is one of the fundamental tools in the study of the noise analysis in the cylinderical shell. Therefore, lot of the acousticians induced many cylinderical shell motion equations[1],[2] Recently, we introduced the newly induced cylinderical Shell Equation and Junger and Feit's shell equation[5], and computed the linear Equation with the supossed solution. In the case of the free motion of the shell, we compared above cylinderical shell equations by using freewavenumber. With this result, we proves the useful of a newly induced cylinderical shell equation and we can analyse the Structure-Borne Sound of the shell with this equation in the application.