경동맥 내막 절제술 후 발생한 과관류 증후군 1예

송희정,박광열,정진상,조수진,이순정,허승,김동익,이광호,이병붕 대한신경과학회 1999 대한신경과학회지 Vol.17 No.2

개에서 심실 조율부위 및 조율주기에 따른 혈역학적 변화

김희열,김재형,노태호,김종진,진승원,유기동,이만영,채장성,홍순조,최규보 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.9

glpD and glpE 유전자의 조절영역 결손변이주기가 전사조절에 미치는 영향

정희태,정수열,최용락 東亞大學校附設遺傳工學硏究所 1996 遺傳工學硏究 Vol.- No.3

glpD와 glpE는 같은 조절 영역 하에서 121 bp 간격을 두고 역방향으로 전사되어지며, 이들 유전자 상류영역에 존재하는 하나의 CRP binding site가 두 유전자의 전사 조절에 관여함을 이미 여러 연구를 통하여 확인되어졌다. 본 연구에서는 glpD와 glpE의 조절영역이 다양한 크기로 결손된 변이주를 작성하였다. 각 변이된 promoter의 발현조절 영향을 보고자 LacZ와의 융합 plasmid를 작성하였고, β-galactosidase 활성을 측정하여 cAMP에 의한 발현조절을 검토하였다. 발현조절 특성을 확인한 결과 CRP binding site 전까지 결손이 된 변이주는 wild와 비슷한 활성이지만 CRP bin-ding site가 없어진 변이주의 경우는 promoter 활성이 크게 감소했고 cAMP에 의한 발현조절은 나타내지 않았다. 한편 CRP의 공통배열을 삽입시키면 회복이 가능하였다. glpE의 조절영역도 glpD와 마찬가지로 다양하게 결손시킨 변이주를 작성하여 promoter 조절활성을 확인한 결과 CRP binding site가 없어진 변이주의 경우는 promoter 활성이 크게 감소하였으며, 결손된 CRP 결합영역을 삽입시키니 cAMP에 의한 전사촉진이 거의 회복되어졌다. The glpD gene encoding gly-3-p dehydrogenase is essential for the aerobic growth of E. coli on glycerol or gly-3-p. The glpE gene, the function of which is unknown, is transcribed divergently with respect to glpD gene. Expression of the adjacent but divergently transcribed glpD and glpE genes is positively regulated by the cAMP-CRP complex. In this study, for a precise investigation of the functional elements in the regulatory region for transcription activation by cAMP-CRP, deletion mutation have been introducted into the regulatory region. The effect of the deletion mutant on transcriptional regulation was tested in vivo by β-galctosidase activity. Deletion mutants in the regulatory region of glpD demonstrated that the presence of the CRP-binding site resulted in an sixfold increase in promoter activity. And also deletion mutants of glpE gene demonstrated that the presence of the CRP-binding site resulted in an eightfold increase in promoter activity. Insertion of 22 bp oligomer in the deletion mutants has shown that the CRP binding site is need for maximal expression of glpD and glpE genes.

경사격벽을 가진 상자형의 해석

鄭熺孝,安光列,李承烈,朴泳珏 경상대학교 생산기술연구소 2001 工學硏究院論文集 Vol.1 No.-

It is necessary at present to adapt the design of bridge structures to transport requirement and the restrictive conditions of environment. City bridges, bridges spanning railway lines and stations, skew crossing of streets and highways, etc., require an irregular arrangement of internal supports. If diaphragms are applied, they are usually skew. It is advantageous to use a box girder as the basic component for a bridge of this kind because the box girder provides a high torsional stiffness, a favorable stresses, and due to the small shear stresses arising due to torsion. This paper deal with the analysis of the function of irregularly situated support and skew diaphragms and with their influence on the resulting state of stress of the box girder. The solution is shown for a box girder under eccentric load and according to the length of skew diaphragms.

개선된 탄성지반상 보의 휨해석

鄭熺孝,安光列,李承烈,朴泳珏 경상대학교 생산기술연구소 2002 工學硏究院論文集 Vol.2 No.-

The BEF(Beam on Elastic Foundation) analogy was first noted by Vlasov and then utilized for the distortional analysis of box girders by Wright et al., but it was limited only to prismatic straight box girders. With the development of this study, a more generalized element in a stiffness matrix form was presented. With this element, which is "equivalent" to the BEF(Beam on Elastic Foundation) analogy, more complicated configurations can be resulted. In this study, it compares analysis results using the traditional trigonometric function to solve a BEF(Beam on Elastic Foundation) problem with analysis results using stiffness matrix form to solve a EBEF(Equivalent Beam on Elastic Foundation).

객체지향 프로그램 종속성 그래프를 이용한 클래스 계층구조의 재구조화에 대한 연구

류희열,권재길 동의공업대학 2001 論文集 Vol.27 No.1

In this paper, we presents a restructuring method for class hierarchies which can optimize member variable and member function using Object-oriented Program Dependence Graph(OPDG). In the progress program evolution, if the member variable and member function is inserted and updated to classes, it can be included the useless code or unappropriate inheritance relationship. The reuse of such classes is caused to the code errors and the increase of maintenance cost. In this paper, we propose a restructuring method that removes the classes, member variables and member functions that don't exist relationship with the used object, variable and methods in main function and removes the same member functions and member variables of subclass as superclass, we can obtain the simplification of program code and the progress of system performance by eliminating unused member variables and member functions, It can be increased to the efficiency of program testing and maintenance.

sfs유전자의 malE유전자 발현 촉진

정수열,이희영,최용락 東亞大學校附設遺傳工學硏究所 1995 遺傳工學硏究 Vol.- No.2

CRP에 변이가 도입된 crp*¹ 유전자를 통하여 cloning된 sfs(sugar fermentation stimulation)유전자 중 nlp와 sfs4 유전자가 maltose 및 maltodextrin의 운반에 관여하는 유전자들 중에서 MalE 단백질을 증가시킴을 immunoblotling을 통하여 확인했다. 즉 MalE 단백질은 periplasmic에 존재하면서 cytoplasmic membrane에 존재하는 MalFGK 단백질과 반응하여 cytoplasm내로 maltose, maltodextrin을 운반하는 MBP(maltose binding protein)로 밝혀져 있다. Most crp* mutants isolated so far were capable of fermenting lactose despite the absence of cAMP, however, they could ferment other sugar such as maltose in the presence of cAMP. nlp and sfs4 genes, which stimulated the maltose metabolism in a crp*¹, cya::km(MK2001) host system. Especially, MalE protein(malE gene: MBP: maltose binding protein), that is transport protein with maltose and maltodextrin in periplasmic, were increased in the cloned with nlp and sfs4 genes.

隔壁의 傾斜에 따른 箱子桁의 硏究

鄭熺孝,安光列,李承烈,朴泳珏 경상대학교 생산기술연구소 2002 工學硏究院論文集 Vol.2 No.-

The box girder has became a popular bridge type for long span or curved bridges because of its structural advantage of torsional stiffness. Generally box girder have inner diaphragms. The purpose of diaphragms is to prevent the deformation of cross section shape. Most diaphragms are parallel to cross section but it is necessary at present to adapt the design of bridge structures to transport requirement and the restrictive conditions of environment. City bridges, bridges spanning railway lines and stations, skew crossing of streets and highways, etc., require an irregular arrangement of internal supports. If diaphragms are applied, they are usually skew. It is advantageous to use a box girder as the basic component for a bridge of this kind because the box girder provides a high torsional stiffness, a favorable stresses, and due to the small shear stresses arising due to torsion. This paper deal with the analysis of the function of irregularly situated support and skew diaphragms and with their influence on the resulting state of stress of the box girder and compare with vertical deflections, horizontal deflections, longitudinal stresses, distortional stresses according to the angle of diaphragms.

관동맥 우회로술 중 경식도 심초음파도로 관찰한 도부타민 투여후와 우회로술후의 좌심실 국소벽운동

김희열,김종진,윤호중,유기동,박지원,이길환,노태호,채장성,김재형,홍순조,최규보 대한순환기학회 1997 Korean Circulation Journal Vol.27 No.8

식품성분표 차이에 따른 섭취 영양소 추정 비교 연구

이심열,백희영 동아시아식생활학회 2000 동아시아식생활학회지 Vol.10 No.3

This study was conducted to investigate the influence of different nutrient databases in estimating nutrient intake. A nutrient survey with Food Frequency Questionnaire containing 65 food items was conducted with 2,426 subjects over 30 years of age living in Yeonchon-gun. Kyungki province. The nutrient intakes were first estimated by using one (A) nutrient database which was based on the Korean Food Composition Table, 4th edition. With the other (B) nutrient database which was based on the Korean RDA (Recommended Dietary Allowances) 6th edition, it was reestimated and two results were compared. For most nutrients except carbohydrate, calcium, vitamin C and 8-carotene, mean nutrient intake level estimated from database B was significantly higher than that from database A(p<0.05). Mean intake level of most nutrients from two databases were significantly correlated by Pearson's correlation coefficients (p<0.001). Results from the ranking of nutrient intake levels of the subjects by two databases were highly correlated (p>0.9, p<0.001). Weighted kappa values representing measures of agreement ranged from 0,55 for vitamin C to 0.97 for carbohydrate. The proportion of subjects classified into the same quintiles by two databases ranged from 45% for vitamin C to 96% for carbohydrate. This result implies that different nutrient database may produce substantial differences in estimating the absolute nutrient intake but may not be crucial in ranking or classifying individuals with regard to specific nutrient intake.