Retinoic acid induces expression of Ig germ line α transcript, an IgA isotype switching indicative, through retinoic acid receptor

Mi-Hee Park,박석래,Mi-Ra Lee,Young-Ha Kim,Pyeung-Hyeun Kim 한국유전학회 2011 Genes & Genomics Vol.33 No.1

Retinoic acid (RA) is considered to possess an activity of IgA isotype switching. Thus far, TGF-β1 is known to be the most powerful IgA isotype switch factor. To elucidate the molecular mechanisms underlying the Ig germ line (GL) α transcriptional regulation by RA, we constructed three different sizes of mouse GLα promoter reporters; short-GLα(-130/+14), middle-GLα(-448/+72) and long-GLα(-3028/+72). Based on luciferase assay, RA increased the activity of all three GLα promoter reporters by approximately 2-fold and the effect was further enhanced by TGF-β1. Overexpression of Smad3/4 increased TGF-β1-induced GLα promoter activities but had no effect on RA-induced GLα promoter activities. In order to analyze the characteristics of the RA-inducible GLα promoter region,we also constructed two mutant reporters: Smad3 binding elements (SBEs)-substituted short-GLα (short-GLα mSBE)and Runx3 binding elements (RBEs)-substituted short-GLα(short-GLα mRBE) promoter reporters. Promoter activities of the two mutant reporters to RA were comparable to that of wild type reporter, while those of the two mutant reporters to TGF-β1 were markedly diminished as compared to that of WT short-GLα. Finally, RA-induced GLα transcription was virtually disappeared by LE540, an antagonist of RA receptor (RAR). Taken together, these results suggest that RA induces GLα transcription mainly through RAR pathway, where neither Smad3/4 nor Runx3 is involved.

류마티스관절염 환자의 인슐린 저항성 결정인자

이정욱 ( Joung Wook Lee ),박영은 ( Young Eun Park ),조미라 ( Mi Ra Cho ),백승훈 ( Seung Hoon Baek ),김근태 ( Geun Tae Kim ),이준희 ( Jun Hee Lee ),김성일 ( Sung Il Kim ) 대한류마티스학회 2009 대한류마티스학회지 Vol.16 No.2

Objective: Rheumatoid arthritis (RA) is associated with an increased cardiovascular events. These may be related to insulin resistance (IR). We evaluated status of IR and analyzed the relationship between IR and clinical and laboratory characteristics in patients with RA. Methods: We examined 52 RA patients (43 females) and 52 age and sex matched healthy controls. We measured Homeostasis model assessment (HOMA) IR, calculated according to fasting serum glucose and insulin. Results: In patients, age was 50.8±10.2 years old, disease duration was 42.1±30.9 months. In controls, HOMA IR was 0.62±0.30 and in patients, it was 1.28±0.50. This difference was highly significant (p<0.001). Patients with early RA (disease duration is less than 36 months) were 28, and established RA (more than 36 months) were 24. HOMA IR was significantly higher in patients with established RA (1.42±0.45) than those with early RA (1.16±0.45) (p=0.03), and significantly correlated with disease duration (r=0.36, p=0.01), BMI (r=0.36, p<0.001), total cumulative prednisolon dose (r=0.34, p=0.01). Disease duration and BMI were independent predictors for HOMA IR (p<0.01, p=0.03). Conclusion: In patients with RA, IR measured by HOMA IR was more significantly increased than that of healthy control and significantly correlated with disease duration, BMI, and total cumulative prednisolon dose; however, the determinants of HOMA IR in RA patients were disease duration and BMI.

고대 한국의 타계관

나희라 한국고대사학회 2003 韓國古代史硏究 Vol.30 No.-

The outlook on another world has appeared and developed diversely in human history. Another world was created by human beings in order to overcome or deny the fact of life, so it involved the world of gods, the world of ideal, the world of the dead, and the world of eternal life. Consequently, the outlook on these worlds happened to be confused or repeated with each other in many ancient cultures. The outlook on another world in ancient Korea varied passing through some phases. The concept on the soul of the dead and another world was not concrete at the phase of immature social hierarchy. However, social complexity and specialization led the thought on the fate of humankind and the world after death to be complicated. Additionally, another world was set up in different forms according to social role or status. For example, only the highest class like king and the nobility was thought to be able to go to the heaven, the supreme another world. The outlook on another world changed again greatly in the process of reorganizing social order as a result of expansion of productivity after 4th century. Increasing self-consciousness about human individualization and grope for new social relationship helped universal outlook on another world which emphasized individual's autonomous will and practice show up. Buddhism and Taoism also influenced on this situation.

의료기기 QI 활동 개선방안에 대한 연구

강훈희,주라형,김종순,김서확,허수진 한국의료QA학회 1998 한국의료질향상학회지 Vol.5 No.2

Background : Medical equipments take a very important role in diagnosis and treatment of disease in modern medicine and effective maintenance of the equipments is a necessory to provide a good healthcare to the public. After developing a new QC program for effective maintenance of medical equipments and practicing it for a year, we report the results of the new program. Methods : The maintenance data of 9 equipments in 8 categories including a CT Scanner were analyzed with regard to the parts responsible for most frequent failure and cause of the failure. After learning the most frequent failure part and cause of the failure, we developed a new QC program that emphasizes preventive maintenance of the most frequent failure part. We compared the number of failure per year and active rate of each equipment before, and after the adoption of the new QC program. Results : The average number of failure per year per equipment was 20.7 before and it decreased by 43% to 11.9 after adoption of the new QC program. The average active rate of the equipments was 92.6% before and it increased by 3.2% to 95.8% after adoption of the new program. Conclusions : The practice of the new QC program appears very useful as it decreased the failure rate and increased the active rate of the equipments.

전국 10개 종합병원 중환자실 환자의 직장내 Vancomycin-resistant Enterococci(VRE) 보균율, 보균의 위험요인 및 유전적 다양성 조사

정희진,송준영,엄중식,김우주,최선주,최정현,이혁,정문현,이광미,신동현,손보라,최종성,우흥정,권정아,이경원,최강원,박승철 대한감염학회 2002 감염 Vol.34 No.5

중년여성의 운동 예측모형

이미라,소희영 충남대학교 간호과학연구소 2002 충남대 간호학술지 Vol.5 No.1

This study was conducted to test the hypothetical model designed to explain exercise behavior of middle-aged women. This hypothetical model was based on behavior-related theories and exercise-related literature. Exogenous variables were exercise habit and perceived subjective norm. Endogenous variables were exercise benefit, exercise barrier, exercise-related affect, exercise self-efficacy, exercise intention, and exercise. A convenience sample of 152 middle-aged women was studied. SAS PC Program and LISREL 8.12a program were used for descriptive statistics and linear structural relationship(LISREL) modeling. The results were as follows. 1)The overall fit of the hypothetical model was acceptable(χ2 34.86(p=0.0015), χ2/df 2.49, RMSEA 0.09, standardized RMR 0.05, GFI 0.95, AGFI 0.85, NFI 0.92, NNFI 0.87, CFI 0.95). 2)The results of statistical testing of hypotheses were as follows. (1)The varibles in this hypothetical model explained 95% of the variance of the exercise behavior. (2)Habit was the most important variable to explain exercise behavior, followed by self-efficacy, barrier, affect and benefit. Subjective norm and intention were not significant predictors of exercise behavior. Habit and self-efficacy had a significant direct effect on exercise behavior. Barrier and intention did not directly influence on exercise behavior. Habit, benefit, barrier and affect had a significant indirect effect on exercise behavior through other variables in this hypothetical model. Norm had no indirect effect on exercise behavior. (3)The direct path between habit and benefit, habit and barrier, habit and self-efficacy, norm and barrier, benefit and affect, barrier and self-efficacy, and affect and self-efficacy were significant. The indirect path between habit and affect, habit and self-efficacy, benefit and self-efficacy, benefit and intention were significant. In conclusion, this study verified that the hypothetical model constructed in this study could be used effectively as a reference for further structural modeling or interventional studies.

사회과를 통한 비판적 사고력 훈련이 비판적 사고력 및 학업성취도에 미치는 효과

황희숙,문미라 釜慶大學校 2002 釜慶大學校 論文集 Vol.7 No.-

The purpose of this study is to examine the effects of embedding approach to teach thinking skills on critical thinking and academic achievement. In embedding approach, critical thinking skills are taught in the context of a subject matter, rather than in isolation. Subjects of this study were 140 middle school 2nd grade students in Busan, Korea who were randomly assigned to two experimental and two control groups. The experimental groups had received critical thinking instruction in a period of 15 weeks. They had received critical thinking instruction by introducing critical thinking skills concurrent with social studies. Control groups were given traditional lessons only by the method of lecture. Students were given Cornell Critical Thinking Test, Inventory of Thinking Disposition, and Academic Achievement Test as pre- and post test measures. T test and ANCOVA were used to analyze data. The results of this study showed that embedding approach to teach critical thinking skills turned out to have a positive influence on critical thinking and academic achievement.

도라지가 난소를 절제한 흰쥐의 혈 중 지질 함량 변화에 미치는 영향

김미향,김원희,박미라,한희선,배송자 신라대학교 자연과학연구소 2003 自然科學論文集 Vol.11 No.-

This study was designed to observe the effects of the feeding Platycodon grandiflorum A.DC(PG) extract on the improvement of the lipids on serum in ovariectomized rats. To investigate the effects of ethanol extract, Sprague-Dawley female rats were randomly assigned to groups as follows: Sham, OVX-control and ovariectomized rats supplemented with PG at 50㎎/㎏ bw/day. Body weight gain was not significantly different in groups. The levels of total cholestcrol was higher in ovaiectornized control rats than Sham-operated rats, but supplementation of PG ethanol extracts at 50mg/kg bw/day decreased the level of cholesterol in surum. These results might be expected that ethanol extract of PG is believed to be possible protective effects for the fatty serum increasing serum lipid.

컴퓨터를 이용한 혈액학 교육시스템의 개발

이기형,손보라,윤희석,조원덕,김승택 충북대학교 의학연구소 2001 忠北醫大學術誌 Vol.11 No.1

연구목적 : 전통적인 의학교육 시스템은 최근 개발되고 있는 여러 가지 영상 진단법 등 시각적 자료를 전달하는데 한계가 있으며, 이러한 한계는 모든 분야에 걸쳐서 나타나지만 특히 혈액학이나 심장학 등 시각적인 자료가 진단에 필수적인 분양에서 두드러진다고 할 수 있다.본 연구는 혈액학 교육에 사용되던 기존의 강의실 교육에 시각적 자료가 첨부된 CD-ROM을 개발하여 혈액학 교육 시스템을 개선하기 위한 것이다. 대상 및 방법 : 기존의 혈액학 교육에 있어서 필수적으로 강의 되어오던 자료를 수집하여 체계적으로 정리하였다.강의 자료에 따라서 필요한 영상 자료를 충북대학교 병원에 입원한 혈액질환 환자들의 검사 자료로부터 수집하여 컴퓨터를 기반으로 한 의학영상 처리 및 분석시스템을 통하여 이미지 파일로 변형하였다.또한 입원 환자들 중 대표적인 증례들을 수집하여 실제 임상 진료와 유사한 형태의 증례를 통한 학습을 할 수 있도록 하였다. 결과: 본 CD-ROM은 혈액학 교육에 필요한 대부분의 영역을 포함한다 각 질환의 병태생리, 진단, 임상양상, 치료 및 예후 등 전통적으로 강의되어 오던 강의록에 이미지 파일로 변형된 영상 자료를 필요한 곳마다 링크시켰다.따라서 학습자는 특별한 장치 없이 텍스트 및 영상을 한 화면에서 볼 수 있어 전통적인 교육 시스템에 비하여 시,공간적인 장점을 갖는다 할 수 있다.또한 입원 환자들로부터 수집된 증례를 통하여 실제 임상 진료와 매우 유사한 경험을 하도록하여 교육효과를 증대시켰다. 결론: 혈액학 교육에 있어서 멀티미디어적 효과를 극대화시켜 질환의 병태생리 등 강의실 교육자료뿐 아니라 진단에 필수적인 시각적 자료를 손쉽게 접할 수 있는 CD-ROM의 개발로 전통적인 강의방법이 갖는 한계를 개선시킬 수 있을 것으로 기대된다. Purpose : The traditional medical education system has its limitations in effectiveness of conveying informations in multiple modalities used in diagnosis of patients with hematologic diseases.This study was performed to develop such a multimedia system using computer based CD-ROM, which includes visual assistant systems, several hematological case studies as well as the traditional text-wise education systems. Materials and Methods: The informations about hematologic diseases were compiled and organized.The main visual data was peripheral blood smears, bone marrow data, and several laboratory results accrued from patients admitted to Chungbuk National University Hospital.Each visual data were transformed through computer-based medical image processing and analyzing system and saved as to JPEG files.Case studies were also acquired from admitted patients and the laboratory data of that patients were used unmodified as possible as. Results : The CD-ROM covers almost all kinds of hematologic diseases that must be included in ordinary medical education courses.The text part includes pathophysiology, diagnosis, clinical features, treatment and prognosis of each hematologic diseases.The essential visual data were linked to each text part and accessible with ease, by one click.Case studies were simulated to actual clinical settings and presented step by step from diagnoses to treatments. Conclusion: A multimedia approach for the study of hematologic diseases has been developed and available in CO-ROM format.This system has advantage over traditional education system in conveying all kinds of visual data which is essential in educating hematologic disorders.

무선 통신망에서 보안 인증 프로토콜 절차

김동국,김희철,나기창,배철수,나상동 조선대학교 전자정보통신연구소 2002 電子情報通信硏究所論文誌 Vol.5 No.1

Recently, We are using B-ISDN(Broadband Integrated Services Digital Network) with backbone based on ATM(Asynchronous Transfer Mode), the rapid incresement of user for Mobile Computing application and conversion to radio ATM networks had brought by mobile phone, portable computer spread using and extension of multimedia service brought multimedia mobile communications service to increasing the demands. ATM networks gives communication services based on protocol stack with communized the features of wire ATM-network on the whole' structure and has structure supported migratory in radio section. In this paper, we are going to look at services of authentication, access control, confidentiality, integrity and key exchange which are used with solution for privacy detection from an intrusion of persons privacy by illegal intrusion and every threat to user and service suppling company. And we are studying existing mechanism about security service, authentication protocol and data integrity supply in connection with packetization and depacketization about protocol. We are supposed to look at the features of existing wire ATM network, security service application in three plane in chapter 2, the features and basic structure about wireless ATM network which is extended to radio section based on wire ATM comparison and analysis about authentication protocol applied to security constituent matters which should improve in chapter 3. we are propose about security authentication protocol in control plane, one of wireless ATM protocol reference model in chapter 4, results and study direction about proposed contents in chapter 5.