Adiponectin Attenuates the Inflammation in Atopic Dermatitis-Like Reconstructed Human Epidermis

( Hee-seok Seo ),( Ki Hyun Seong ),( Chang-deok Kim ),( Seong Jun Seo ),( Byung Cheol Park ),( Myung Hwa Kim ),( Seung-phil Hong ) 대한피부과학회 2019 Annals of Dermatology Vol.31 No.2

Background: Atopic dermatitis (AD) is a chronic disorder, with a vicious cycle of repetitive inflammation and deterioration of the epidermal barrier function. Adiponectin, an adipokine, has anti-inflammatory effects on various metabolic and inflammatory disorders. Recently, its level was found to be reduced in serum and tissue samples from AD patients. Objective: We aimed to investigate the effects of adiponectin on epidermal inflammation and barrier structures in AD skin. Methods: A three-dimensional in vitro epidermal equivalent model mimicking AD was obtained by adding an inflammatory substance cocktail to normal human epidermal equivalents (HEEs). The expression of epidermal differentiation markers, primary inflammatory mediators, and lipid biosynthetic enzymes was compared between adiponectintreated AD-HEEs, untreated control AD-HEEs, and normal HEEs. Results: Adiponectin co-treatment 1) inhibited the increase in mRNA expression of major inflammatory mediators (carbonic anhydrase II, neuron-specific NEL-like protein 2, thymic stromal lymphopoietin, interleukin-8, tumor necrosis factor-alpha, and human beta-defensin-2) from keratinocytes in AD-inflammatory HEEs, 2) enhanced the expression of lipid biosynthetic enzymes (fatty acid synthase, HMG CoA reductase, and serine-palmitoyl transferase), and 3) promoted the expression of differentiation factors, especially filaggrin. We also found that the expression of adiponectin receptor-1 and -2 decreased in the epidermis of chronic AD lesion. Conclusion: Activation of the adiponectin pathway is expected to enhance epidermal differentiation and barrier function as well as attenuate inflammatory response to AD as a therapeutic approach. (Ann Dermatol 31(2) 186∼195, 2019)

기저질환이 없는 사람에서 발생한 Campylobacter fetus에 의한 재발성 심내막염 1예

전재범,정진원,김성혜,송문희,오형철,안성기,최상호,김남중,김양수,우준희,류지소 대한감염학회 2004 감염과 화학요법 Vol.36 No.6

C. fetus 심내막염은 드물게 발생하는 질환으로 주로 면역능이 저하된 환자에서 발병하고 대동맥판을 침범하는 경우가 많으며 적절한 치료가 되지 않을 경우 치사율이 높다. 저자들은 기저질환이 없던 69세 남자에서 C. fetus 심내막염을 경험하였기에 문헌고찰과 함께 보고하는 바이다. 환자는 발열로 내원하여 시행한 혈액배양 검사상 C. fetus가 동정되었고 경식도 초음파 검사상 삼첨판에 증식증이 발견되어 심내막염 진단하에 ceftriaxone에 이어 meropenem으로 총 4주간 항생제를 투여받고 호전되었다가 한달 후 다시 재발하였고 판막부전 소견 진행하여 삼첨판 치환술을 시행받고 전신상태 호전되어 퇴원하였다. Campylobacter fetus is a rare cause of endocarditis. To date, only about 30 cases of C. fetus endocarditis have been reported mainly in the elderly with chronic underlying illnesses. Most cases showed tendency to involve aortic valve. This report describes a case of native tricuspid valve infective endocarditis in a 69-year-old man without any underlying disease. Despite adequate antibiotic therapy for 4-weeks, endocarditis recurred and leading to surgical valve replacement.

상자형의 전단지연 현상과 유효폭에 관한 연구

鄭熺孝,金洪俊,李承烈,朴峻熙 경상대학교 생산기술연구소 2002 工學硏究院論文集 Vol.2 No.-

In a box girder, it is an accepted practice in structural engineering to represent the effect of 'shear lag' by adopting an 'effective width' concept. The actual width of flange plate (b) is replaced by a reduced width (b_e) over which the longitudinal stresses may be considered uniformly distributed and the application of the elementary theory of bending to the transformed girder cross section gives the correct value of maximum longitudinal stress (σ_max). This paper provides how to determine the magnitude of 'shear lag' and 'effective width ratio' in case of orthotropic box girder which is simply supported, symmetrically loaded only.

만성 승모판 패쇄부전증 환자에서의 안지오텐신 전환효소 억제제의 장기 투여 효과

김대희,이명묵,이해영,조현재,박승정,서재빈,서정원,양한모,윤창환,조상호,이준희,김용진,김명아,손대원,오병희,박영배 대한순환기학회 2004 Korean Circulation Journal Vol.34 No.2

케이슨 변위 발생 원인분석 및 보강대책 사례

남준희(Nam, Jun?Hee),김완호(Kim, Wan-Ho),김우규(Kim U-Gyu),이강일(Lee, Kang-Il) 한국지반신소재학회 2019 한국지반신소재학회 학술발표회 Vol.2019 No.10

철근콘크리트 T형교의 내하력 증진

남문희,정희효,이관희,박준용 慶尙大學校生産技術硏究所 1996 生産技術硏究所論文集 Vol.12 No.-

Even though there are a lot of methods of promotion of load resistance for reinforced concrete T-beam bridge, the methods have been faults respectively. In this study, we will convert simple supported T-beam into continuous T-beam for promotion of load resistance and cancel joint. This method is decidedly superior to another method in maintenance, construction, economics and external appearance.

품종별 감꽃 동결건조 분말의 유효성분 분석

김준한 ( Jun Han Kim ),박석희 ( Seok Hee Park ),문혜경 ( Hye Gyeong Mun ),이인선 ( In Seon Lee ),김종국 ( Jong Kuk Kim ) 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2006 한국식품저장유통학회지 Vol.13 No.6

감 품종별(도근조생, 고종시, 갑주백목, 차랑, 월하시, 상주둥시)로 감꽃을 개화기에 채취하여 동결건조 분말화한 감꽃분말에 함유된 유효성분을 분석, 비교하였다. 조지방 함량은 도근조생이 57.26%로 가장 높은 값을 보였으며, 유리당 조성은 fructose가 95~310 mg%, glucose는 75~281 mg%, sucrose가 7~28 mg%의 함유량을 나타내었고, 유기산 중 malic acid가 갑주백목(6월4일)에 225 mg%로 가장 많았고, tartaric acid도 189 mg%로 가장 많았다. 유리아미노산 중 hydroxy-L-proline은 월하시에 25.33 mg%, L-citrulline은 상주둥시(5월28일)가 58.83 mg%, L-threonine은 상주둥시(5월28일)가 11.88 mg%로 가장 많았다. 페놀산으로 caffeic acid는 갑주백목(6윌4일)이 1946 μg/100 g, phydroxybenzoic acid는 상주둥시(5월29일)가 417.8 μg/100 g, protocatechuic acid는 갑주백목(6월1일)이 181.1 μg/l00 g으로 가장 많았다. 따라서 감꽃에도 감이나 감잎에 못지않게 유용한 식품성분들과 기능성 성분들이 다량 함유되어 있어 새로운 식품 및 기능성 신소재로서의 가치가 매우 높다고 할 수 있다. This study was carried out to analyze useful components in freeze-dried persimmon flower powder made from six cultivars. The cultivars were Dogunjosang, Kojongsi, Kabjubaekmok, Chalang, Weolhasi and SangjuDungsi. Powder of persimmon flower was prepared by milling after freeze drying at -70℃. Crude lipid was the highest in Dogunjosang (57.26%). Major free sugars of the persimmon flowers were fructose (95~310 mg%), glucose (75~281 mg%)and sucrose (7~28 mg%). Major organic acids were malic acid (225 mg% in Kabjubaekmok (Jun. 4th)) and tartaric acid (189 mg% in Kabjubaekmok (Jun. 4th)). Predominant free amino acids were hydroxy-L-proline(25.33 mg% in Weolhasi), L-citrulline (58.83 mg% in SangjuDungsi (May 28th)) and L-threonine (11.88 mg% in SangjuDungsi (May 28th)). Major phenolic compounds in the persimmon flowers were caffeic acid (1,946 μg/100g in Kabjubaekmok (Jun. 4th)), p-hydroxybenzoic acid(418 μg/100g in SangjuDungsi (May 29th)) and protocatechuic acid(181 μg/100g in Kabjubaekmok(Jun. 1st)). The results suggest that persimmon flowers be potential materials as useful food ingredients.

프리미돈의 한국인에서의 생체이용율시험

권광일,윤민혁,윤희열,박희찬,권준택,심희옥,김동출 충남대학교 약학대학 의약품개발연구소 2004 藥學論文集 Vol.19 No.-

A reversed phase HPLC method was developed and validated for the determination of primidone in human plasma. Propylthiouracil was used as an internal standard. Calibration curves were linear in the concentration range of 10-120 ng/㎖. The coefficient of variation of the intra- and inter-day precision were below 15%. The coefficient of variation of the accuracy were below 15% in the concentration range investigated. A bioavailability study was performed using the validated HPLC method. Twenty four healthy human male volunteers were orally administered 20 mg of primidone. The pharmacokinetic parameters were calculated using WinNonlin. The mean values of AUC_(LAST) was 85.0±17.7 ㎍ hr/㎖, C_(max) was 4.22±1.04 ㎍/㎖, T_(max) was 1.85±1.13 hr, t_(1/2) was 17.7±2.4 hr. The pharmacokinetic parameters and the HPLC method can be used for the desigm of bioequivalence study of primidone.

다발성 대장암 간전이 환자의 간절제를 위한 새로운 시도

주종우,김형철,임철완,신응진,조규석,유기원,송옥평,홍대식,박성진,조준희,이혜경,김희경,권계원,고은석 순천향의학연구소;Soonchunhyang Medical Research Institute 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

Multiple bilobar liver matastases (MBLM) are the main cause of low resectability in the colon cancer liver metastases. The authors experienced one case of initially non-resectable colon cancer liver metastases. He was curatively and safely treated with a two-stage hepatectomy using the new method of future remnant liver volume growing. A 54-year-old man was referred to our department with the sigmoid colon cancer combined with MBLM, which were checked in two small metastatic lesions in the left lobe and five large sized lesions in the right lobe in the computed tomogram (CT). A laparoscopic assisted anterior resection was primarily performed. We performed the 1^(st) stage hepatectomy 3 weeks after the colon resection. Intra-operative Ultrasonogram (US) found 9 small superficial metastatic lesions in the left lobe. All that lesions were completely removed by non-anatomical wedge resection. An occlusion ballon catheter was placed in the right portal vein through a small branch of the inferior mesenteric vein at that time. The future remnant liver volume was sufficiently increased 3 weeks after the 1^(st) hepatectomy. A right hepatectomy was safely performed 22 days after the 1^(st) hepatectomy. The patient received a regional chemotherapy (interleukin2 based immuno-chemotherapy through hepatic artery) for 4 months, then received 9 cycles of systemic chemotherapy (biweekly Oxaliplation, leucovorin, plus 5-fluorouracil) without any recurrence evidence.

흰쥐 Kupffer 세포에 대한 Propofol의 작용

박세훈,지대림,성언기,김희선,송인환,박희영,김준수,이덕희,박대팔 대한마취과학회 2002 Korean Journal of Anesthesiology Vol.43 No.4

Background: Propofol has an antioxidant capacity and can be used for ischemia-reperfusion injury of the liver. However, the effects of propofol on the Kupffer cells have not been establisked. Methods: Kupffer cells were isolated and cultured from male Sprague-Dawley rats. The effects of propofol on the Kupffer cells were evaluated by a phagocytosis assay, TNF-α gene expression, and superoxide anion release after administering propofol in different concentrations on the cultured Kuprrer cells. Results: The latex bead phagocytosis by the Kupffer cells was suppressed when the Kupffer cells were exposed to propofol irrespective of concentrations. Higher propofol concentrations decreased the loss of Kupffer cells after latex bead phagocytosis. Propofol induced TNF-α mRNA expression in the Kupffer cells, but the mRNA expression level after 50㎍/ml of propofol decreased. The pattern of TNF-α mRNA expression induced by propofol was different to that induced by LPS: TNF-α mRNA was expressed continuously in the propofol-treated cells until 16 hours after exposure to propofol, whereas the level of TNF-α mRNA expression induced by LPS was evident after 2 hours and was not found thereafter. TNF-α production after propofol treatment was not higher than that of the control. Formazan presipitation did not show any qualitative differences between cells untreated or treated with propofol concentrations of 0.5, 5.0, and 50 ㎍/ml. Conclusions: These results showed that propofol might inhibit Kupffer cells. This suggests that propofol and be used for patients with ischemia-reperfusion injury of the liver. (Korean J Anesthesiol 2002; 43: 475~484)