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청열해독용 한약재의 항진균작용에 의한 항감염성 관절염 효과검색
공수현,이주영,박지혜,김송이,이주희,한용문 동덕여자대학교 종합약학연구소 2007 동덕약학연구지 Vol.11 No.-
In present study, we tested antifungal effects of 11 different kinds of compounds isolated from Phellodendri Cortex (PC), Lonicerae Flos (LF), Lonicerae Folium et Caulis (LFC) and Araliae Continental is Radix (ACR). These herbal medicines are generally applied for the treatment of inflammations. Prior to the test, antifungal effect of 70%-ethanol extracts of the medicines was examined. Results showed that the three extracts of PC, LF and LFC, but not ACR extract, inhibited Candida albicans growth, in-vitro. Among the 11 compounds, berberine and palmatine isolated from PC showed the most antifungal activity (P < 0.05). Lonicerin and loganin from LF and chlorogenic acid from LFC also retarded growth of C. albicans at high dose of 250 ㎛/ml, but their activity was much less than berberine or palmatine. However, ent-Pimara-8(14),15-diene-19-oic acid and 16α,17- Dihydroxy-ent-kauran-19-oic acid from ACR had no antifungal activity as expected. All together, these data imply that most of oriental herbal medicines having anti-inflammatory activity could have anticandidal effect, suggesting that PC, LF and LFC be effective for the treatment of septic arthritis by killing of C. albicans.
Chlorogenic acid Rutin의 Macrophage 및 T-임파구 증식에 대한 효과
박지혜,공수현,이주영,김송이,이주희,한용문 동덕여자대학교 종합약학연구소 2007 동덕약학연구지 Vol.11 No.-
In the present study, we examined immunoregulatory activitres of cholrogenic acid (CA) and rutin that are isolated from Lonicera Flos (A family of Caprifoliaceae). For the examination, NO production of LPS-treated RAW264.7 monocyte/macrophage cell line and effect on the T-cells proliferation were determined. Results showed that CA inhibited NO production at low concentrations below 10 ㎛/ml, but at higher concentration ranged from 10 ㎛/ml to 40 ㎛/ml the inhibitory activity was lost. However, rutin blocked the NO production in a dose-dependancy. In case of T-cell proliferation, both of the compounds suppressed the proliferation (P < 0.05). Combined together, it is assumed that the compounds might be effective to arthritic inflammation, possibly, by removal of CD4+ T-cells, but rutin might be more effective against the inflammation because of the blockage of NO production from macro phages which is responsible for the pain during the process of arthritis.
An Algorithm for Finding a Reasonable Smooth Curve for Any Given Data
Hee-Jue Eun,Suk-Jin Kim,Yong-Sung Kim 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.1
This paper suggests an assignment algorithm in order to construct a reasonable Slope for any given data along with building division algorithms for generating interpolating intervals. In connection with these, finding out the Smooth Curve algorithm and generating it are essential. Finally, the fulfillment of the mathematical error analysis and numerical experience concerning with Smooth curve algorithm result in a reasonably varitying the Smooth Curve.
Jue-Hee Lee 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.7
Arthritis due to pathogenic fungi is a serious disease causing rapid destruction of the joint. In the pathogenesis of arthritis, T lymphocytes are considered to be one of the major immune cells. In present study, we examined the T cell immunoregulatory effect by ochnaflavone (Och), a biflavonoid, on arthritis caused by Candida albicans that is the most commonly associated with fungal arthritis. To examine the effects of ochnaflavonon Candida albicans-caused septic arthritis, an emulsified mixture of C. albicans cell wall and complete Freund's adjuvant (CACW/CFA) was injected into BALB/c mice via hind footpad route on days -3, -2, and -1. On Day 0, Och at 1 or 2 mg/dose/time was intratraperitoneally given to mice with the swollen footpad every other day for 3 times. The footpad-edema was measured for 20 days. Results revealed that Och reduced the edema at all dose levels and furthermore, there was app. 45% reduction of the edema in animals given 2 mg-dose at the peak of septic arthritis (p < 0.05). This anti-arthritic effect was accompanied by the diminishing of the DTH (delayed type hypersensitivity) activity against the CACW and by the provoking of the dominant T helper 2 (Th2) type cytokines production (IL-4 and Il-10), which appeared to result in a suppression of T helper 1 cytokines (IFN-γ and IL-2). Besides the T cell immunoregulatory activity, Och inhibited T cells activation as evidenced by the IL-2 reduction from PMA/ionomycin-stimulated Jurkat cell line and in addition, the compound killed macrophages in a dose-dependent manner (p < 0.05). However, Och caused no hemolysis (p < 0.05). These data implicate that Och, which has anti-arthritic activity based on the Th2 dominance as well as macrophage removal, can be safely administered into the blood circulation for treatment of the arthritis caused by C. albicans. Thus, it can be concluded that Och would be an ideal immunologically evaluated agent for treating of Candida arthritis.
황련 유래 Antimicrobial Peptide의 Candida albicans 감염 억제효과
이주희(Jue-Hee Lee) 대한약학회 2011 약학회지 Vol.55 No.3
We previously reported the protein isolated from Coptidis Rhizoma (CRP), which has antifungal activity against a fungal pathogen, Candida albicans. In the current study, we investigated what portion in the CRP is responsible for the antifungal activity. For the investigation, the CRP was fractionated on a Shepadex G-50 column. Data resulting from the fractionation, seven fractions were obtained. Fractions (Fr.) I, II, and III eluted initially from the column showed no inhibitory effect on the growth of C. albicans, whereas Fr. IV, V, and VI eluted later revealed inhibition of the growth, and Fr. IV and VI showed potent antifungal activity by broth susceptibility analysis. However, Fr. VI was contained in the CRP more than Fr. IV, which led us to select the VI for the following experiments. In a murine model of a subcutaneous candidiasis caused by C. albicans, the Fr. VI displayed a therapeutic effect on nude mice pretreated with anti-neutrophil monoclonal antibody (RB68C5) and then infected subcutaneously with live C. albicans. At day 16, these mice were healed almost up to 78% of the infected area when compared to infected area of control nude mice that received diluent (Dulbecco's Phosphate-Buffered Saline; DPBS), instead of the Fr. VI (P<0.01). The Fr. VI blocked hyphal formation from blastoconidial form of C. albicans (P<0.01), which might prevent penetration of hyphae to the deeper site of skin and thus helps the healing. In the ionic strength test, the effect of Fr. was influenced by Ca2+ ion just like other known antimicrobial peptides, but the influence was affected at an extremely high concentration such as 500 mM. Thus, such ion-concentration is considered to be meaningless in the clinical situation. Considering all data together, Coptidis Rhizoma is appeared to produce an antimicrobial peptide that has therapeutic effect on subcutaneous infection caused by C. albicans.
이주희(Jue Hee Lee),한용문(Youngmoon Han) 대한약학회 2005 약학회지 Vol.49 No.6
Streptococcus pneumoniae is the leading cause of pneumonia and bacterial meningitis. The current polysaccharide vaccine has been reported ineffective in elderly adults and children less than 2 years of age. Thus, in recent many researchers have been focusted on a different approach, DNA vaccine. In our laboratory, we developed a Streptococcus pneumoniae DNA(SPDNA) vaccine. This SPDNA vaccine was formulated by inserting the resion encoding part of the capsule in the S.pneumoniae into the LAMP-1. In present work, with use of the SPDNA vaccine we attmpted to to establish a certain methodology useful for evaluation of effectiveness and immunoresponse of a DNA vaccine. Results showed that the subcutaneous route was the most effective for production of antisera specific for S. pneumoniae in mice. By isotyping analyses, IgM, IgG1, IgG2a, and IgG2b were determined. In addition, INF-γ and IL-4 were predominantly detected. Combination of those data resulted in a pattern of IgG1IL-4, which indicates the immunity towards the Th1 response predominantly. Furthermore, the SPDNA vaccination induced resistance of the CD4+T lymphocyte-depleted mice against disseminated pneumococcal infection. These data appear to be possibly due to activation of CD8+T cell-activation. Taken together, this methodology can be applied for evaluating efficacy and mode of action of a DNA vaccine as minimum criteria.
Jung, Hee-Won,Chung, Chun Kee,Chi, Je G.,Bang, Yung Jue,Heo, Dae Seog,Ahn, Yoon-Ok,Kim, Il Han,Kim, Noe Kyeong,Choi, Kil Soo,Han, Dae Hee 대한신경외과학회 1992 Journal of Korean neurosurgical society Vol.21 No.9
악성 뇌교종환자에서 수술후 방사선 치료와 함께 시작하여 최소 2주기이상 시행한 ACNU 화학요법이 방사선치료만 시행하였을때보다 관해유지에 부가되는 치료효과가 있는지를 결정하기 위하여 전향성 무작위 임상시험을 시행하였다. 수술후 악성 뇌교종으로 확진된 환자들을 연령, 성별, 수술적출 정도 및 병리 소견으로 충화한 다음, 무작위로 방사선치료 단독군(단독군)(11명) 또는 방사선 및 화학요법 병용군(병용군)(11명)으로 할당하였다. 연령 분포상 50세 이상이 45% 이었고 남녀비는 1 : 1이었다. 전적출은 단독군에서 2례, 병용군에서 5례 시행하였으며 악성 성상세포종이 27%, 다형성교모세포종이 73%이었다. 이러한 연령, 성별, 수술적출의 정도 및 병리소견의 분포는 단독군과 병용군간에 차이가 없었다. 총관해율(완전 및 부분)은 단독군에서 36.4%, 병용군에서 27.3%였다. 환자의 생존기간 및 병의 진행기간을 카프란-마이어법으로 분석하였고 단독군과 병용군 간의 차이는 로그-순위법으로 비교하였다. 일년 누적생존율은 단독군에서 60.6%, 병용군에서 46.7%로 두군간의 차이는 관찰되지 않았다(0.25<p<0.50). 그러나 70주이상의 장기 누적 비진행율은 단독군(22.7%)에서보다 병용군(46.7%)에서 더 높았다. 병용군의 64%에서 부작용(주로 골수억제)이 관찰되었고, 단독군의 36%에서 오심이 관찰되었다. 화학요법제의 용량조절이 필요한 경우가 병용군의 64%에서 관찰되었으나, 골수억제기간이 일시적이었으므로 이로인하여 화학요법의 중단이 필요한 경우는 없었다. 본 초기보고상에서는 ACNU가 종양관해유도 및 관해유지에 통계학적으로 유의한 부가적 효과가 있다고 하기는 어렵다. 그러나, 장기적인 종양관해의 유지에 미치는 부가적 효과에 대한 판정은 환자수가 더 축적되고 추적관찰의 기간이 연장되면 가능할 것으로 판단되었다.
이주희(Jue Hee Lee),심진기(Jin Kie Shim),한용문(Young Moon Han) 대한약학회 2005 약학회지 Vol.49 No.5
Our previous data showed the protein isolated from Coptidis Rhizoma(CRP) had antifungal activity. In present study, we examined mode of action of the CRP and its activity against subcutaneous candidiasis 엳 새 C. albicans yeast cells. Results showed that the CRP blocked hyphal production from yeast form of C. albicans. The CRP also activated RAW 264.7 monocyte/macrophage cell line, which resulted in nitiric oxide (NO) production from the cells. This activation seemed to increase macrophage phagocytosis to destroy the invaders. Like other antimicrobial peptides, CRP was influenced by ionic strength, thus resulting in a decrease of antifungal activity. In murine model of a subcutaneous candidiasis, the sizes of infected areas of the nude mice given the CRP after subcutaneous injection of C. albicans yeast cells to the dorsal skin were 90% less than those of the nude mice groups that received DPBS instead of the CRP. All data indicate that the CRP, which appeared to act like an antimicrobial peptide and to inhibit the morphological transition from blastoconidia, was effective against the subcutaneous disease.
Candida albicans Can Utilize Siderophore during Candidastasis Caused by Apotransferrin
Lee Jue-Hee,Han Yong-Moon The Pharmaceutical Society of Korea 2006 Archives of Pharmacal Research Vol.29 No.3
Ability of iron acquisition of pathogenic microorganisms functions as a virulence factor. Candida albicans, a fungal pathogen that requires iron for growth, is susceptible to growth retardation by high-affinity iron binding proteins such as transferrin. Recently, we reported that C. albicans could utilize the heme as a part of heme-containing proteins dissociated by heme oxygenase, CaHMX1. In search of another pathway that C. albicans can use to bypass the growth regulation produced by iron limitation, this present study examined utilization of non-candidal siderophores such as Desferal and rhodotorulic acid (RA) for acquisition of inorganic iron by the fungus. C. albicans secreting no siderophores was cultured in iron-free (pretreated with apotransferrin for 24 h) (culture medium). Once growth of the yeast reached stasis from iron starvation, a siderophore was added to the culture media. Results showed that cultures containing apotransferrin within a dialysis membrane recovered growth to the level of untreated controls, whereas C. albicans yeast cells in direct contact with soluble iron-free (apo) transferrin recovered growth only partially. When static growth from iron limitation was reached, the addition of siderophore-apotransferrin complex to culture medium also permitted the yeast to recover growth from apotransferrin growth regulation. All the data show that C. albicans can utilize the non-candidal siderophores for iron acquisition under transferrin regulation as can pathogenic bacteria.