Transient Receptor Potential Canonical 4 and 5 Channel Antagonist ML204 Depolarized Pacemaker Potentials of Interstitial Cells of Cajal

( Jun Hyung Lee ),( Wen-hao Wu ),( Xing-you Huang ),( Jae Yeoul Jun ),( Seok Choi ) 대한소화기기능성질환·운동학회(구 대한소화관운동학회) 2020 Journal of Neurogastroenterology and Motility (JNM Vol.26 No.4

Background/Aims To investigate an effect of ML204 (an inhibitor of transient receptor potential canonical 4 and 5 [TRPC4/5] channels) on interstitial cells of Cajal (ICCs) and therefore determine whether TRPC4/5 channels act on ICC-generated pacemaker activity. Methods We enforced whole cell patch clamp analysis, measurements of the intracellular Ca2+ concentration, and reverse transcription polymerase chain reaction to determine the effect of ML204 (10 μM) or englerin A (a selective activator of TRPC4/5 channeles, 10 μM) and the existence of TRPC4/5 in mouse small intestinal ICC. Results Treatment of ICCs with ML204 or englerin A caused the membrane potentials to depolarize. This depolarization effect of membrane potentials by ML204 in ICCs was observed to be concentration-dependent. After treating Ca²⁺- and Na⁺-free solutions or flufenamic acid (a non-selective cation channel blocker), the pacemaker potentials in the ICCs were abolished. A specific anoctamin 1 channel blocker did not have any effect on the pacemaker activity in ML204-untreated control cells; however, they blocked ML204-induced pacemaker activity in ICCs. Specific primers designed against TRPC4 and TRPC5 detected the presence of TRPC4/5 in small intestinal ICCs, and the application of ML204 increased raise the frequency of Ca²⁺ oscillations in ICCs, as assessed using Fluo-4 AM. Conclusion The results implied that ML204 could not inhibit the pacemaker activity but depolarized the membrane potential of ICCs by regulating intracellular Ca²⁺ oscillations and anoctamin 1 channels. (J Neurogastroenterol Motil 2020;26:521-528)

Kinematic Stability of Tunnel Face in Non-uniform Soils

Jun-Hao Zhong,Xiao-li Yang 대한토목학회 2020 KSCE JOURNAL OF CIVIL ENGINEERING Vol.24 No.2

In the paper, the kinematic analysis is carried out to evaluate the face stability of shallow tunnel in the framework of plasticity theory. In view of the non-uniformity of soil strength parameters, a discretization technique is introduced to generate possible failure mechanisms which respect the associative flow rule and requirement of the non-uniformity of soil strength parameters. As an earthquake situation is considered, a pseudo-dynamic method is adopted to portray the distribution of seismic acceleration. Some comparisons are made to the conventional analysis, including discretization-based pseudo-static analysis and log-spiral-based pseudo-static analysis, to prove the validity of the programming code and accuracy of the proposed method. The soil strength parameters are considered varying in the vertical direction. The effect of distribution of strength parameters is discussed, and distribution form is initially assumed to be linear profile and, then, to be other nonlinear functional profile, including power profile, constant profile and quadratic profile. The results derived from further parametric study show that although the influence of horizontal seismic coefficient is more significant than the vertical one, the vertical effect cannot be ignored in most circumstances, and some factors affect the stability cyclically. In addition, in the linear profile of soil strength parameters, the influence of gradient on supporting pressure is rather small comparing to the average. The influence of non-uniformity of soil strength parameters and seismic parameters on the shape and collapsed area of the failure mechanism is successively studied in this paper. All the optimized solutions are presented in the figures and tables for practical application.

Comparative Study of DC Breakdown and Space Charge Characteristics of Insulation Paper Impregnated with Natural Ester and Mineral Oil

Hao, Jian,Zou, Run-Hao,Liao, Rui-Jin,Yang, Li-Jun,Liao, Qiang,Zhu, Meng-Zhao The Korean Institute of Electrical Engineers 2018 Journal of Electrical Engineering & Technology Vol.13 No.4

Natural ester is a suitable substitute for mineral oil and has been widely used in AC transformer in many countries. In order to further application of natural ester in direct current (DC) equipment, it is needed to investigate its long term insulation property under DC condition. In this paper, a thermal ageing experiment was conducted for both mineral oil-paper and natural ester-paper insulation. The DC breakdown and space charge characteristics of insulation paper impregnated with natural ester and mineral oil was compared. Results show that the resistivity of the paper immersed in natural ester and mineral oil both increase as the ageing goes on. While insulation paper impregnated with natural ester has higher resistivity and DC breakdown voltage than the paper impregnated with mineral oil. The DC breakdown voltage for the oil impregnated insulation paper being DC pre-stressing is higher than that without pre-stressing. The average DC breakdown field strength difference between the test with pre-stressing and without pre-stressing clearly shows that there is an apparent enhancement effect for the homo-charge injection on the DC breakdown.

G-protein Coupled Estrogen Receptor 1 Expression in Primary Breast Cancers and Its Correlation with Clinicopathological Variables

Hao-jun Luo,Ping Luo,Guang-lun Yang,Qiong-le Peng,Man-ran Liu,Gang Tu 한국유방암학회 2011 Journal of breast cancer Vol.14 No.3

Purpose: G-protein coupled estrogen receptor 1 (GPER) probably play important roles in the progression of breast cancer including endocrine therapeutic resistance. We evaluated GPER in primary breast cancers. Methods: Immunohistochemistry was used to detect GPER in paraffin-embedded tissues of primary breast cancers from 423 patients and GPER expression was correlated with clinicopathological factors. Results: GPER was expressed in 63.8% of specimens, coexpressed with estrogen receptor alpha (ERα) in 36.6% of tumors and was positive in 62.5% of the ERα-negative tumors. The expression of GPER had no relationship with the status of ERα, progesterone receptor and HER2. Although the expression of GPER was significantly inversely related with nodal status (p=0.045), no correlation between GPER expression and other clinicopathological variables (age, menstruation status, tumor size, stage, histologic grade, Nottingham Prognostic Index or pathological type) was found. Conclusion: GPER and ERα exhibited independent expression pattern of distribution in primary breast cancers. A long-term follow-up and a more definite molecular phenotype for ER are necessary in confirming studies.

Crystal Structures and Thermal Properties of Two Binuclear Cd(II) Supramolecular Complexes Based on Quinolinecarboxylate Ligand

Hao, Hu-Jun,Yin, Xian-Hong,Lin, Cui-Wu,Wei, Shui-Qiang Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.9

Two novel binuclear metal-organic coordination complexes $[Cd_2(L)_2(bpy)_2(H_2O)_2]{\cdot}6H_2O$ (1), $[Cd_2(L)_2(phen)_2-(H_2O)_2]{\cdot}2H_2O$ (2) (where L = 2-methylquinoline-3,4-dicarboxylate dianion, bpy = 2,2'-bipyridine, phen = 1,10-phenanthroline) have been synthesized under hydrothermal conditions and characterized by single crystal Xray diffraction, spectral method (IR), elemental analysis and thermal gravimetric analysis (TGA). Both 1 and 2 consist of two Cd(II) atoms bridged by two monoatomic bridging carboxylate groups from two L ligands, and the second carboxylate group of each L is monodentately coordinated to Cd(II), creating a sevenmembered chelating ring. The coordination at each metal nucleus is completed by a water molecule and a chelating bidentate molecule. The 3D structures of the complexes are stabilized by ${\pi}-{\pi}$ stacking interactions and hydrogen-bonds.

Stability Analysis of 3D Deep Tunnel Face under Steady Seepage Flow Condition

Jun-Hao Zhong,Hong Liao 대한토목학회 2022 KSCE Journal of Civil Engineering Vol.26 No.5

The instabilities of tunnel face may occur while shields advance through the rivers or region rich in water. It is of significance to attach the importance to the estimation of face stability during the tunnel excavation. Thus, this paper adopted a three-dimensional (3D) rotational mechanism to analyze the face stability under steady seepage flow field with the aid of the kinematic method in the context of limit analysis. The hydraulic head that derived by fitting the results of trial and error is employed herein to portray the steady seepage flow field. With the aid of a double integration method, the work rate induced by seepage forces acting on the soil skeleton is calculated and accordingly a closed-form solution of support pressure is derived based on the work balance equation. By comparing with the literature, one can validate the rationality and correctness of the present results. The research shows that the proposed method based on rotational mechanism provides a better upper bound estimation, when under seepage condition, the influence of internal frictional angle on the stability of the excavation face is not always monotonic and the influence of head difference on the support pressure is significant. Finally, the normalized stability charts are provided for a quick assessment for practical use during tunnel excavation in water-rich stratum.

연구원 활동 : 발표논문요약 < 전기공학분야 >

(Jun Hao Xu),(Guo Guang Zheng),(A . M . Grishin),(B . M . Moon),(K . V . Rao) 고려대학교 공학기술연구소 1995 생산기술연구소보 Vol.2 No.-

Early Diagnosis and Management of Cerebral Venous Flow Obstruction Secondary to Transsinus Fracture after Traumatic Brain Injury

Wen-hao Wang,Jun-ming Lin,Fei Luo,Lian-shui Hu,Jun Li,Wei Huang 대한신경과학회 2013 Journal of Clinical Neurology Vol.9 No.4

Background and Purpose Cerebral venous flow obstruction (CVFO) is a fatal complication of traumatic brain injury. To compare the outcomes of patients with CVFO secondary to traumatic-brain-injury-induced transsinus fracture who were diagnosed early versus those diagnosed late in the therapeutic course. Methods In total, 403 patients with transsinus fracture were reviewed retrospectively. The patients were divided into an early-diagnosis group (n=338) and a delayed-diagnosis group (n=65).The patients submitted to 2D time-of-flight magnetic resonance venography (2D-TOF MRV) and/or CT venography (CTV), depending upon the findings of intracranial pressure monitoring, in order to identify potentially complicated CVFO. These examinations took place within 3 days of the onset of malignant intracranial hypertension symptoms in the early-diagnosis group, and after an average of 7 days in the delayed-diagnosis group. Once diagnosed, patients received intravenous thrombolytic therapy with low-dose urokinase. Patients with massive transsinus epidural hematoma, depressed fracture, or cerebral hernia were treated surgically to relieve the compression and repair any damage to the venous sinuses. Results Cerebral venous flow obstruction was much more severe in the delayed-diagnosis group than in the early-diagnosis group (p<0.001), and hence patients in the former group were given a higher dose of urokinase (p<0.001) for thrombolytic therapy. They were also significantly more likely to need surgery (48.1% vs. 20.6%, p=0.003) and had a higher mortality rate (37.0%vs. 4.1%, p<0.001). However, patients in both groups experienced a similarly favorable prognosis, not only with regard to functional outcome but also with respect to neuroradiological improvement, as evaluated by 2D-TOF MRV/CTV at the final follow-up (p=0.218). Conclusions Delayed diagnosis can result in increased risk of surgery and death in the acute phase. Thrombolytic therapy with low-dose urokinase resulted in promising improvements in both functional and neuroradiological outcomes in all of the patients in this study, regardless of the time to diagnosis. J Clin Neurol 2013;9:259-268

Physicochemical and Sensory Characterization of Ginger-juice Yogurt during Fermentation

Guo-Hao Yang,Jun-Jun Guan,Jin-Shui Wang,Haicheng Yin,Fa-Dong Qiao,Feng Jia 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.6

The primary purpose of this study was to investigate the effects of a ginger (Zingiber ofcinale) juice addition to milk on the physicochemical and sensory properties and consumer acceptability of yogurt during fermentation. Under the presence of ginger juice at 2.0 to 10.0%(v/v), there was a significant influence (p<0.05) on the viability of characteristic microorganisms in yogurt,the pH, titratable acidity (ºSH), viscosity, and hardness of the products. The descriptive data showed significant differences (p<0.05) among the samples for all attributes. The consumer results from external preference mapping indicated that samples with the lower ginger juice (≤4%)were liked over the higher samples (≥6%), with a negative impact on the acceptance by the consumers. Therefore,these findings suggest that 4%(v/v) may be the more appropriate content of ginger juice in the preparation of ginger-juice yogurt.

Global prevalence of depression and anxiety in patients with hepatocellular carcinoma: Systematic review and meta-analysis

Darren Jun Hao Tan,Sabrina Xin Zi Quek,Jie Ning Yong,Adithya Suresh,Kaiser Xuan Ming Koh,Wen Hui Lim,Jingxuan Quek,Ansel Tang,Caitlyn Tan,Benjamin Nah,Eunice Tan,Taisei Keitoku,Mark D. Muthiah,Nichola 대한간학회 2022 Clinical and Molecular Hepatology(대한간학회지) Vol.28 No.4

Background/Aims: Depression and anxiety are associated with poorer outcomes in patients with hepatocellular carcinoma (HCC). However, the prevalence of depression and anxiety in HCC are unclear. We aimed to establish the prevalence of depression and anxiety in patients with HCC. Methods: MEDLINE and Embase were searched and original articles reporting prevalence of anxiety or depression in patients with HCC were included. A generalized linear mixed model with Clopper-Pearson intervals was used to obtain the pooled prevalence of depression and anxiety in patients with HCC. Risk factors were analyzed via a fractional-logistic regression model. Results: Seventeen articles involving 64,247 patients with HCC were included. The pooled prevalence of depression and anxiety in patients with HCC was 24.04% (95% confidence interval [CI], 13.99–38.11%) and 22.20% (95% CI, 10.07–42.09%) respectively. Subgroup analysis determined that the prevalence of depression was lowest in studies where depression was diagnosed via clinician-administered scales (16.07%;95% CI, 4.42–44.20%) and highest in self-reported scales (30.03%; 95% CI, 17.19–47.01%). Depression in patients with HCC was lowest in the Americas (16.44%; 95% CI, 6.37–36.27%) and highest in South-East Asia (66.67%; 95% CI, 56.68–75.35%). Alcohol consumption, cirrhosis, and college education significantly increased risk of depression in patients with HCC. Conclusions: One in four patients with HCC have depression, while one in five have anxiety. Further studies are required to validate these findings, as seen from the wide CIs in certain subgroup analyses. Screening strategies for depression and anxiety should also be developed for patients with HCC.