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( Hanseul Oh ),( C Yoon Kim ),( Chang Hwan Kim ),( Gyeung Haeng Hur ),( Jae Hak Park ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.9
Francisella tularensis is a highly virulent pathogen of humans and other mammals. Moreover, F. tularensis has been designated a category A biothreat agent, and there is growing interest in the development of a protective vaccine. In the present study, we determine the in vitro and in vivo immune responses of a subunit vaccine composed of recombinant peptides Tul4 and FopA from epitopes of the F. tularensis outer membrane proteins. The recombinant peptides with adjuvant CpG induced robust immunophenotypic change of dendritic cell (DC) maturation and secretion of inflammatory cytokines (IL-6, IL-12). In addition, the matured DCs enabled ex vivo proliferation of naive splenocytes in a mixed lymphocyte reaction. Lastly, we determined the in vivo immune response by assessment of antibody production in C57BL/ 6 mice. Total IgG levels were produced after immunization and peaked in 6 weeks, and moreover, Tul4-specific IgG was confirmed in the mice receiving peptides with or without CpG. Based on these results, we concluded that the recombinant peptides Tul4 and FopA have immunogenicity and could be a safe subunit vaccine candidate approach against F. tularensis.
Repellent Properties of Artemisia Annua against Drosophila Fruit Flies
Hanseul Cho 국제과학영재학회 2016 APEC Youth Scientist Journal Vol.8 No.1
Drosophila, also known fruit flies are amongst the most commonly fruit damaging insects. Chemical treatments will provide only a temporary relief and possess chemicals that harm human health. An annual Korean weed, Artemisia annuaL., has been used as a folk medicine for the treatment of anumber of diseases. In this study, the ethanol extract of A. annua (EEAA) was evaluated for the repellent activities against Drosophila fruits flies. 50 anesthetized flies were placed within a 500ml plastic beaker. The number of fruit flies on the petri dish contained extraction and the behavior of the fruit flies were observed within 5, 10 and 20 minutes time. The results suggest that EEAA possess repellent activities against Drosophila fruit flies.
Hanseul Shim,Gyeongrok Kim,정시온,박기수 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.4
This study presents a single-line absorption spectroscopy method for the simultaneous measurement of gas pressure and temperature through diode laser absorption spectroscopy using a single absorption line. In the proposed method, the absorbance loss of an absorption line that may occur during baseline subtraction is predicted, and the gas properties are determined by matching a calculated absorbance to a recovered absorbance using the predicted loss. To verify the proposed method, gas property measurements are conducted for air conditions with a pressure range of 10-100 kPa and a temperature range of 300-500 K. An absorption line in the oxygen A band is selected to measure the gas properties of air. Using the proposed method, it is confirmed that the gas pressure and temperature could be measured within errors of 25 % and 18 %, respectively. When the gas pressure is predetermined using a pressure gauge, the gas temperature can be measured within an error of 14 %.
Humanized Mice for the Evaluation of Francisella tularensis Vaccine Candidates
( Hanseul Oh ),( C-yoon Kim ),( Chang-hwan Kim ),( Gyeung-haeng Hur ),( Ji Min Lee ),( Seo-na Chang ),( Jae-hak Park ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.1
Francisella tularensis (FT), a highly infectious pathogen, is considered to be a potential biological weapon owing to the current lack of a human vaccine against it. Tul4 and FopA, both outer membrane proteins of FT, play an important role in the bacterium’s immunogenicity. In the present study, we evaluated the immune response of mice―humanized with human CD34+ cells (hu-mice)―to a cocktail of recombinant Tul4 and FopA (rTul4 and rFopA), which were codon-optimized and expressed in Escherichia coli. Not only did the cocktail-immunized hu-mice produce a significant human immunoglobulin response, they also exhibited prolonged survival against an attenuated live vaccine strain as well as human T cells in the spleen. These results suggest that the cocktail of rTul4 and rFopA had successfully induced an immune response in the hu-mice, demonstrating the potential of this mouse model for use in the evaluation of FT vaccine candidates.
Recent Progress in Regulation of Aging by Insulin/IGF-1 Signaling in Caenorhabditis elegans
Hanseul Lee,Seung-Jae V. Lee 한국분자세포생물학회 2022 Molecules and cells Vol.45 No.11
Caenorhabditis elegans has been used as a major model organism to identify genetic factors that regulate organismal aging and longevity. Insulin/insulin-like growth factor 1 (IGF- 1) signaling (IIS) regulates aging in many species, ranging from nematodes to humans. C. elegans is a nonpathogenic genetic nematode model, which has been extensively utilized to identify molecular and cellular components that function in organismal aging and longevity. Here, we review the recent progress in the role of IIS in aging and longevity, which involves direct regulation of protein and RNA homeostasis, stress resistance, metabolism and the activities of the endocrine system. We also discuss recently identified genetic factors that interact with canonical IIS components to regulate aging and health span in C. elegans. We expect this review to provide valuable insights into understanding animal aging, which could eventually help develop anti-aging drugs for humans.
Hydrolysis Modification of Tannic Acid as a Biocidal and Antioxidant Agent with Low Cytotoxicity
Hanseul Kim,Pradeep Kumar Panda,Kambiz Sadeghi,Jongchul Seo 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
Tannic acid (TA) is a polyphenolic hydrolyzable tannin having several biological properties. However, the application of TA is limited to its performance, rendering it essential to modify TA to increase its practical applications. This study reports the facile modification of TA by thermal and hydrolysis treatments using autoclave and hydrothermal reactor, respectively. The structures of thermally treated TA (TTA) and hydrolyzed TA (HTA) were determined by FTIR and ¹H NMR. The results confirmed the partial conversion of TTA and complete conversion of HTA into gallic acid and pyrogallol, respectively. TTA and HTA showed improved antimicrobial activity, potent antioxidant activity, and lower cytotoxicity than TA. However, HTA exhibited the highest antimicrobial activity because of the high surface area and cell interaction. Compared to TA and TTA, HTA demonstrated a better antioxidant activity and lower cytotoxicity owing to the steric hindrance and lesser interaction between human cells and pyrogallol. Thus, HTA with potent functional properties can be prepared by a facile processing method and employed as an additive for food packaging and biomedical applications.