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Hansen, H.G.,Pristovsek, N.,Kildegaard, H.F.,Lee, G.M. Pergamon Press ; Elsevier Science Ltd 2017 Biotechnology advances Vol.35 No.1
<P>Chinese hamster ovary (CHO) cells are the preferred cell factory for the production of therapeutic glycoproteins. Although efforts primarily within bioprocess optimization have led to increased product titers of recombinant proteins (r-proteins) expressed in CHO cells, post-transcriptional bottlenecks in the biosynthetic pathway of r-proteins remain to be solved. To this end, the ectopic expression of transgenes (effector genes) offers great engineering potential. However, studies on effector genes have in some cases led to inconsistent results. Whereas this can in part be attributed to product specificity, other experimental and cellular factors are likely important contributors to these conflicting results. Here, these factors are reviewed and discussed with the objective of guiding future studies on effector genes. (C) 2016 Elsevier Inc. All rights reserved.</P>
Hansen, Keith S.,Gutwein, Luke G.,Hartman, Brett C.,Sood, Rajiv,Socas, Juan Korean Society of Plastic and Reconstructive Surge 2016 Archives of Plastic Surgery Vol.43 No.5
Autologous breast reconstruction utilizing a perforator flap is an increasingly popular method for reducing donor site morbidity and implant-related complications. However, aberrant anatomy not readily visible on computed tomography angiography is a rare albeit real risk when undergoing perforator flap reconstruction. We present an operative case of a patient who successfully underwent a bilateral breast reconstruction sourced from a unilateral abdominal flap divided into deep superior epigastric artery and superficial circumflex iliac artery flap segments.
Dynamics of false vacuum bubbles: beyond the thin shell approximation
Hansen, Jakob,Hwang, Dong-il,Yeom, Dong-han IOP Publishing Ltd 2009 Journal of high energy physics Vol.2009 No.11
We numerically study the dynamics of false vacuum bubbles which are inside an almost flat background; we assumed spherical symmetry and the size of the bubble is smaller than the size of the background horizon. According to the thin shell approximation and the null energy condition, if the bubble is outside of a Schwarzschild black hole, unless we assume Farhi-Guth-Guven tunneling, expanding and inflating solutions are impossible. In this paper, we extend our method to beyond the thin shell approximation: we include the dynamics of fields and assume that the transition layer between a true vacuum and a false vacuum has non-zero thickness. If a shell has sufficiently low energy, as expected from the thin shell approximation, it collapses (Type 1). However, if the shell has sufficiently large energy, it tends to expand. Here, via the field dynamics, field values of inside of the shell slowly roll down to the true vacuum and hence the shell does not inflate (Type 2). If we add sufficient exotic matters to regularize the curvature near the shell, inflation may be possible without assuming Farhi-Guth-Guven tunneling. In this case, a wormhole is dynamically generated around the shell (Type 3). By tuning our simulation parameters, we could find transitions between Type 1 and Type 2, as well as between Type 2 and Type 3. Between Type 2 and Type 3, we could find another class of solutions (Type 4). Finally, we discuss the generation of a bubble universe and the violation of unitarity. We conclude that the existence of a certain combination of exotic matter fields violates unitarity.
Biocatalytic Synthesis of Pikromycin, Methymycin, Neomethymycin, Novamethymycin, and Ketomethymycin
Hansen, Douglas A.,Rath, Christopher M.,Eisman, Eli B.,Narayan, Alison R. H.,Kittendorf, Jeffrey D.,Mortison, Jonathan D.,Yoon, Yeo Joon,Sherman, David H. American Chemical Society 2013 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.135 No.30
<P>A biocatalytic platform that employs the final two monomodular type I polyketide synthases of the pikromycin pathway in vitro followed by direct appendage of <SMALL>d</SMALL>-desosamine and final C–H oxidation(s) in vivo was developed and applied toward the synthesis of a suite of 12- and 14-membered ring macrolide natural products. This methodology delivered both compound classes in 13 steps (longest linear sequence) from commercially available (<I>R</I>)-Roche ester in >10% overall yields.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/2013/jacsat.2013.135.issue-30/ja404134f/production/images/medium/ja-2013-04134f_0008.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ja404134f'>ACS Electronic Supporting Info</A></P>