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Hanhong Bae,Lakshmi Narayanan Ponpandian,Soon Ok Rim,Gnanendra Shanmugam,Junhyun Jeon,Buyng Su Hwang,Junheon Kim,Seon Keun Lee,Sang-Tae Seo,Sang-Hyun Koh 한국응용곤충학회 2018 한국응용곤충학회 학술대회논문집 Vol.2018 No.10
Pine trees are ecologically important in Korea. They are seriously imperiled by Pine wilt disease (PWD), by pine wood nematode (PWN, Bursaphelenchus xylophilus). Here, we isolated and characterized bacterial endophytes (BEs) from pine trees in Korea for biological control of PWN using BE metabolites. Using culture-dependent approach BE isolates were extracted from three tissues (needles, stems, and roots) of four pine species across 18 sampling sites in Korea. Bacterial isolates were characterized into 389 distinct isolates based on 16S rDNA sequencing. Ethyl acetate crude extracts (CEs) of bacterial liquid cultures were prepared using ethyl acetate and screened for nematicidal activity against PWN. BEs (1,622 isolates) were isolated; their taxonomic binning resulted in 215 operational taxonomic units (OTUs). Analysis of species richness and Shannon’s diversity of the three tissues revealed that BEs colonized the needles more than the stem and root tissues. Furthermore, based on nematicidal activity screening of 389 isolates, 44 BEs were identified, with two isolates exhibiting a significant inhibitory activity against PWN. Taken together, these data revealed numerous nematicidal BEs in pine trees, providing new insights that can serve as an effective and promising alternative approach to combat PWD.
Bae, Hanhong,Sicher, Richard C.,Kim, Moon S.,Kim, Soo-Hyung,Strem, Mary D.,Melnick, Rachel L.,Bailey, Bryan A. Oxford University Press 2009 Journal of experimental botany Vol.60 No.11
<P><I>Theobroma cacao</I> (cacao) is cultivated in tropical climates and is exposed to drought stress. The impact of the endophytic fungus <I>Trichoderma hamatum</I> isolate DIS 219b on cacao's response to drought was studied. Colonization by DIS 219b delayed drought-induced changes in stomatal conductance, net photosynthesis, and green fluorescence emissions. The altered expression of 19 expressed sequence tags (ESTs) (seven in leaves and 17 in roots with some overlap) by drought was detected using quantitative real-time reverse transcription PCR. Roots tended to respond earlier to drought than leaves, with the drought-induced changes in expression of seven ESTs being observed after 7 d of withholding water. Changes in gene expression in leaves were not observed until after 10 d of withholding water. DIS 219b colonization delayed the drought-altered expression of all seven ESTs responsive to drought in leaves by ≥3 d, but had less influence on the expression pattern of the drought-responsive ESTs in roots. DIS 219b colonization had minimal direct influence on the expression of drought-responsive ESTs in 32-d-old seedlings. By contrast, DIS 219b colonization of 9-d-old seedlings altered expression of drought-responsive ESTs, sometimes in patterns opposite of that observed in response to drought. Drought induced an increase in the concentration of many amino acids in cacao leaves, while DIS 219b colonization caused a decrease in aspartic acid and glutamic acid concentrations and an increase in alanine and γ-aminobutyric acid concentrations. With or without exposure to drought conditions, colonization by DIS 219b promoted seedling growth, the most consistent effects being an increase in root fresh weight, root dry weight, and root water content. Colonized seedlings were slower to wilt in response to drought as measured by a decrease in the leaf angle drop. The primary direct effect of DIS 219b colonization was promotion of root growth, regardless of water status, and an increase in water content which it is proposed caused a delay in many aspects of the drought response of cacao.</P>
Yi Wang,Hanhong Jiang,Pengxiang Xing 전력전자학회 2018 JOURNAL OF POWER ELECTRONICS Vol.18 No.1
To enable a master–slave control independent microgrid system (MSCIMGS) to supply electricity continuously, the microgrid inverter should perform mode transfer between grid-connected and islanding operations. Transient oscillations should be reduced during transfer to effectively conduct a seamless mode transfer. This study uses a typical MSCIMGS as an example and improves the mode transfer strategy in three aspects: (1) adopts a status-tracking algorithm to improve the switching strategy of the outer loop, (2) uses the voltage magnitude and phase pre-synchronization algorithm to reduce transient shock at the time of grid connection, and (3) applies the hybrid-sensitivity H∞ robust controller instead of the current inner loop to improve the robustness of the controller. Simulations and experiments show that the proposed strategy is more practical than the traditional proportional–derivative control mode transfer and effective in reducing voltage and current oscillations during the transfer period.
Partial Fault Detection of an Air - conditioning System by using a Moving Average Neural Network
Doyoung Han,Hanhong Lee 대한설비공학회 2003 International Journal Of Air-Conditioning and Refr Vol.11 No.3
The fault detection and diagnosis technology may be applied in order to decrease the energy consumption and the maintenance cost of the air-conditioning system. In this paper, two fault detection methods were considered. One is a generic neural network, and the other is an moving average neural network. In order to compare the performance of fault detection results from these methods, two different types of faults in an air-conditioning system were applied. These are the condenser 30% fouling and the evaporator fan 25% slowdown. Test results showed that the moving average neural network was more effective for the detection of partial faults in the air-conditioning system.
Role of Dehalogenases in Aerobic Bacterial Degradation of Chlorinated Aromatic Compounds
Arora, Pankaj Kumar,Bae, Hanhong Hindawi Limited 2014 Journal of chemistry Vol.2014 No.-
<P>This review was conducted to provide an overview of dehalogenases involved in aerobic biodegradation of chlorinated aromatic compounds. Additionally, biochemical and molecular characterization of hydrolytic, reductive, and oxygenolytic dehalogenases was reviewed. This review will increase our understanding of the process of dehalogenation of chlorinated aromatic compounds.</P>
AUTOSAR와 MOST 네트워크를 연동하기 위한 응용 수준 게이트웨이 컴포넌트의 설계
박대동(Daedong Park),금한홍(Hanhong Keum),박지용(Jiyong Park),홍성수(Seongsoo Hong) 한국자동차공학회 2009 한국자동차공학회 부문종합 학술대회 Vol.2009 No.4
As the size and the complexity of automotive software increase rapidly, the use of a standardized software platform becomes mandatory. AUTOSAR is the de facto standard for automotive software platform developed by leading car manufacturers and first-tier suppliers. However, AUTOSAR has limited applicability since it only supports CAN, LIN and FlexRay which are mostly used for vehicle control applications. It does not support the MOST communication protocol that is commonly used for in-vehicle infotainment applications. This prevents AUTOSAR-based control applications from communicating with the infotainment applications. In order to solve the problem, we propose an application-level gateway component that interconnects AUTOSAR and MOST networks. Our gateway allows applications on one network to communicate with applications on the other network even though the applications do not know the protocol of the opposite side network. Moreover, our gateway is designed not to require any extensions or modifications to the AUTOSAR standard. In this paper, we give a detailed design of the internal structure and the protocol conversion mechanism of the gateway.
승용차용 재제조 시동전동기의 신뢰성 향상을 위한 성능평가시스템 개발
정남훈(Namhoon Chung),이한홍(Hanhong Lee) 한국자동차공학회 2008 한국자동차공학회 춘 추계 학술대회 논문집 Vol.- No.-
Recent settlement of the industrial society of mass production and mass consumption brings about an exhaustion of natural resources and global warming. Therefore, various researches are under way for the pursuit or economic growth and environmental protection at the same time. For example, directive on ELV went into effect on January 2006 in EU in order to increase the reuse and recovery rate for vehicles. In our country, the resources circulation law of automobile is established for the promotion of reuse of automotive components and effective on January 2008. In this paper, remanufacturing, which is one of the reuse of automotive parts, is introduced and performance evaluation system of the remanufactured starter motor is proposed for the quality certification.