Production of Hypoallergenic Cocoa Beans by a Pregermination Treatment Method

Handoyo, Tri,Mitsugu, Akagawa,Kim, Kyung-Min The Korean Society of Plant Biotechnology 2015 식물생명공학회지 Vol.42 No.2

Pregermination treatment of cocoa beans either with the testa, group PCB (+T), or without the testa, group PCB (-T), was studied here to determine whether this treatment (incubation up to 120 h at $25^{\circ}C$) has any effect on the levels of allergenic proteins or on chemical composition. Our proximate analysis included carbohydrates, proteins, and lipids. We used western blotting to measure changes in the amounts of allergenic proteins in the cocoa beans during the pregermination treatment. The protein and carbohydrate content of both groups (with or without the testa) decreased slightly during this treatment, whereas lipid content increased. Group PCB (-T) showed more rapid metabolic processes than did group PCB (+T) during the pregermination treatment. Western blot analysis showed that the cocoa beans contained an allergenic protein of ~28 kDa. Removal of the testa strongly reduced the amount of this allergenic protein after 72 h of the pregermination treatment. Generally, the pregermination treatment increased antioxidant activity in both groups. Significant differences in the antioxidant activity were observed between groups PCB (-T) and PCB (+T). Particularly, group PCB (-T) showed high antioxidant activity at 72 h of the pregermination treatment. Thus, the combination of cocoa beans without the testa and pregermination treatment (72 h) seems to be the optimal method for production of hypoallergenic cocoa beans rich in antioxidants for patients with allergic disorders.

Production of Hypoallergenic Cocoa Beans by a Pregermination Treatment Method

Tri Handoyo,Akagawa Mitsugu,김경민 한국식물생명공학회 2015 식물생명공학회지 Vol.42 No.2

Pregermination treatment of cocoa beans either with the testa, group PCB (+T), or without the testa, group PCB (-T), was studied here to determine whether this treatment (incubation up to 120 h at 25°C) has any effect on the levels of allergenic proteins or on chemical composition. Our proximate analysis included carbohydrates, proteins, and lipids. We used western blotting to measure changes in the amounts of allergenic proteins in the cocoa beans during the pregermination treatment. The protein and carbohydrate content of both groups (with or without the testa) decreased slightly during this treatment, whereas lipid content increased. Group PCB (-T) showed more rapid metabolic processes than did group PCB (+T) during the pregermination treatment. Western blot analysis showed that the cocoa beans contained an allergenic protein of ~28 kDa. Removal of the testa strongly reduced the amount of this allergenic protein after 72 h of the pregermination treatment. Generally, the pregermination treatment increased antioxidant activity in both groups. Significant differences in the antioxidant activity were observed between groups PCB (-T) and PCB (+T). Particularly, group PCB (-T) showed high antioxidant activity at 72 h of the pregermination treatment. Thus, the combination of cocoa beans without the testa and pregermination treatment (72 h) seems to be the optimal method for production of hypoallergenic cocoa beans rich in antioxidants for patients with allergic disorders.

Determinants of Export Diversification in Developing Countries

Rossanto Dwi Handoyo,Solihin,Kabiru Hannafi Ibrahim 대한산업공학회 2021 Industrial Engineeering & Management Systems Vol.20 No.4

This study unravels the impact of different determinants of export diversification in 62 developing countries classified as low, middle, and high-income over the period 2010-2018. The empirical strategy based on Poisson Pseudo-Maximum Likelihood revealed that GDP promotes the diversification of export in low, middle, and all countries’ samples and reduces it in high-income countries. Human capital reduces export diversification in low and middle-income countries and increases it in high-income and all countries’ samples. Population and countries’ competitiveness are associated with increased export diversification. Additionally, innovation does not affect diversification in all the country classifications while R&D significantly promotes diversification in middle-income and reduces it in low-income and all countries sample. The mediation effects of the variables are positive in the middle-income and high-income countries and mixed effect in low-income and all countries sample. The study, therefore recommends the need to develop human capital, increase global competitiveness, optimal use of resources for R&D to further increase export diversification in developing countries.

Sarcasm Detection in Twitter - Performance Impact While Using Data Augmentation: Word Embeddings

Alif Tri Handoyo,Hidayaturrahman,Derwin Suhartono,Criscentia Jessica Setiadi 한국지능시스템학회 2022 INTERNATIONAL JOURNAL of FUZZY LOGIC and INTELLIGE Vol.22 No.4

Sarcasm is the use of words commonly used to ridicule someone or for humorous purposes. Several studies on sarcasm detection have utilized different learning algorithms. However,most of these learning models have always focused on the contents of expression only, thusleaving the contextual information in isolation. As a result, they failed to capture the contextualinformation in the sarcastic expression. Moreover, some datasets used in several studies havean unbalanced dataset, thus impacting the model result. In this paper, we propose a contextualmodel for sarcasm identification in Twitter using various pre-trained models and augmentingthe dataset by applying Global Vector representation (GloVe) for the construction of wordembedding and context learning to generate more sarcastic data, and also perform additionalexperiments by using the data duplication method. Data augmentation and duplication impactis tested in various datasets and augmentation sizes. In particular, we achieved the bestperformance after using the data augmentation method to increase 20% of the data labeledas sarcastic and improve the performance by 2.1% with an F1 Score of 40.44% compared to38.34% before using data augmentation in the iSarcasm dataset.

Klappertaart: an Indonesian–Dutch influenced traditional food

Chrisfella Cokro Handoyo,Clarissa,Grace Claudia,Milka,Syahnazia A. Firdayanti 한국식품연구원 2018 Journal of Ethnic Foods Vol.5 No.2

Background: As one of the most ethnically diverse societies, Indonesia is well known for its cultural diversity such as traditional practices and customs. Some of them are historically affected by colonization. Indonesia experienced a colonial history under the Dutch rule for a long time. It is considered as a long colonial period that has left huge influences on various aspects of Indonesian people's lives and Indonesian cuisine. One of Indonesian food that is influenced by Dutch culture is Klappertaart. Klappertaart in Indonesian literally means “kue kelapa” or coconut cake that is usually served during special events. This study aims to analyze the influence of the Dutch culture on Indonesian dish, klappertaart. Methods: The method of this research is literature study history about Indo-Dutch food culture. Results: As a result of colonization, many Dutch tribes set foot in many regions of Indonesia. At that time, the Dutch traders who came to Manado, North Celebes, introduced a recipe of a dish that has later been combined with Indonesian ingredients. Because of that, the ingredients of klappertaart is a combination of Dutch and Indonesian culture. That is what makes klappertaart associated with Manado. Conclusions: Klappertaart is one of the results of acculturation between Dutch and Indonesian culture. Colonization in Indonesia not only gave a negative impact, but also improves Indonesian food culture.

Stock Returns Response to Internal and External Shocks during the COVID-19 Pandemic in Indonesia: A Comparison Study

Rossanto Dwi Handoyo,Kabiru Hannafi Ibrahim,Frandy Yosza Indrawan 대한산업공학회 2022 Industrial Engineeering & Management Systems Vol.21 No.1

This study uses impulse response function and variance decomposition from the Vector Error Correction Model (VECM) and analyses the response of Jakarta Composite Index (JCI) return to the shocks of oil price, gold price, the exchange rate, interbank interest rates, COVID-19 cases, and the stock market index of Malaysia, Singapore, Thailand, Japan, and the United States. Daily secondary data were used for the analysis and our empirical strategy from the impulse response function divulges that JCI return responds positively to the shock of the Malaysia and Japan stock indices and negatively to Singapore, Thailand, and the United States stock indices. Our finding further reveals that JCI return responds positively to its shock, shocks of the gold price, exchange rates, and negatively respond to the shocks of oil price, interbank rate, and COVID-19 cases. Therefore, based on the study findings policy recommendations are made to mitigate the negative influence of the shocks variables on JCI return.

QTL mapping related to grain shattering using DH population in rice (Oryza sativa L.)

Gyu-Ho Lee,Hyun-Suk Lee,Tri Handoyo,Fika Ayu Safitri,A Ra Cho,Kyung-Min Kim 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07

The seed shattering played a key role in the crucial step of rice domestication. Because it has been important to increase the yield human had to select the rice varieties and species with low shattering degree. The shattering habit of rice is considered to be under the relatively simple genetic control compared with other characteristics related to domestication. Several recessive genes associated with the formation of an abscission layer, sh2, sh4 and sh-h on chromosomes 1, 3 and 7, have been reported. In addition, the grain shattering of rice is considered to be caused by seed abscission. The morphology of the abscission layer can differ in many different rice varieties that show varying degrees of shattering. Accordingly, it is important to elucidate the molecular mechanism to determine why some varieties do not have abscission layers and have an easy-shattering trait. In this study, analysis of QTL for grain shattering was performed to determine the location of QTLs on the whole chromosomes of rice. Also, we tried to construct a physical map for qPs6

Photovoice in the English as an Additional Language (EAL) Writing Classroom : No Need to Rush to Love Writing Because Love Will Grow with Time

Sandi Ferdiansyah,Handoyo Puji Widodo,Tariq Elyas 아시아영어교육학회 2020 The Journal of Asia TEFL Vol.17 No.1

Primary School English Teachers’ Perceptions of the Teacher Educator-Created English Textbook: The Case of Indonesia

Herlina Usman,Sandi Ferdiansyah,Handoyo Puji Widodo,Reni Puspitasari Dwi Lestariyana 아시아영어교육학회 2020 The Journal of Asia TEFL Vol.17 No.3

Genetic Diversity Analysis of Indonesian Aromatic Rice Varieties (Oryza sativa L.) Using RAPD

Nur Meili Zakiyah,Tri Handoyo,김경민 한국작물학회 2019 Journal of crop science and biotechnology Vol.22 No.1

In this research, we investigated genetic diversity of 21 rice genotypes (Oryza sativa L.) assessed using 38 decamer RAPD primers. A total of 405 bands were produced from 38 primers, which revealed that 84.44% was polymorphic and 15.56% was monomorphic. From the first cluster, Batang Gadis separated from 14 other genotypes. The second cluster consisted of six genotypes which all had awn in the seed except Gogo Fatuk Masin. The high number of amplified polymorphism bands showed that the markers can be used to distinguish the rice genotype well. RAPD markers can show differences in individual fingerprint patterns, since genetic variation is important for the maintenance and development of the organism's potential. The information about the genetic diversity in this study was useful for plant breeders in the selection of elders and processing of plant cultivation.