Effects of Potato By-products Containing Glycoalkaloid on Rumen Fermentation Characteristics

Dimas Hand Vidya Paradhipta,이혁준,주영호,이성신,강동훈,정기용,김삼철 경상국립대학교 농업생명과학연구원 2020 농업생명과학연구 Vol.54 No.4

The present study was conducted to investigate the effects of feed types (powder vs. pellet) from potato by-product containing glycoalkaloid on rumen fermentation with different rumen pH (low vs. high) using in vitro technique. Rumen fluid was collected from two cannulated Hanwoo heifers before morning feeding (high rumen pH at 6.71) and 3 h after feeding (low rumen pH at 6.20). A 0.5 gram of powder or pellet from potato by-product was incubated in the rumen buffer for 48 h at 39℃. Four replications for each treatment were used along with two blanks. Pellet had higher (p<0.05) the immediately degradable fraction, the fractional degradation rate, and the lag phase than those of powder. However, feed type had no effects on the potentially degradable fraction and the total degradable fraction. In fermentation characteristics, powder had higher (p<0.05) concentrations of ammonia-N, total volatile fatty acid (VFA), and acetate than those of pellet. In addition, a high rumen pH had higher (p<0.05) concentrations of ammonia-N and acetate, but lower (p<0.05) total VFA concentration than those of a low rumen pH. The interaction effects between feed type and rumen pH were found on the total degradable fraction and concentrations of ammonia-N, total VFA, and acetate. The present study concluded that generally feed type had no effects on rumen degradation kinetics, but potato by-product in the powder form is recommended due to produce higher fermentation characteristics than in the pellet form.

북한이주민을 활용한 학교 통일교육 프로그램 개발

배한동 ( Hand Dong Bae ),성장환 ( Jang Hwan Sung ),김영하 ( Young Ha Kim ) 경북대학교 중등교육연구소 2005 중등교육연구 Vol.53 No.3

Effects of wild or mutated inoculants on rye silage and its rumen fermentation indices

Paradhipta, Dimas Hand Vidya,Joo, Young Ho,Lee, Hyuk Jun,Lee, Seong Shin,Kwak, Youn Sig,Han, Ouk Kyu,Kim, Dong Hyeon,Kim, Sam Churl Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.6

Objective: This study was conducted to confirm the effects of new inoculants producing-antifungal or esterase substances on rye silage and its rumen fermentation indices by comparing wild with mutated types. Methods: Rye harvested at dough stage was ensiled into 3 L mini bucket silo (1 kg) for 90 d in triplicate following: distilled water at 20 μL/g (CON); Lactobacillus brevis 100D8 (AT) and its inactivation of antifungal genes (AT-m) at 1.2×10⁵ cfu/g, respectively; and Leuconostoc holzapfelii 5H4 (FD) and its inactivation of esterase genes (FD-est) at 1.0×10⁵ cfu/g, respectively. After silo opened, silage was sub-sampled for the analysis of ensiling quality and its rumen fermentation indices. Results: Among the wild type inoculants (CON vs AT vs FD), FD inoculant had higher (p<0.05) in vitro digestibilities of dry matter and neutral detergent fiber, the total degradable fraction, and total volatile fatty acid in rumen, while AT inoculant had higher (p<0.05) lactate, acetate, and lactic acid bacteria in silage. Silage pH and the potentially degradable fraction in rumen increased (p<0.05) by inactivation of antifungal activity (AT vs AT-m), but lactate, acetate, and lactic acid bacteria of silage decreased (p<0.05). In silage, acetate increased (p<0.05) by inactivation of esterase activity (FD vs FD-est) with decreases (p<0.05) of pH, ammonia-N, lactate, and yeast. Moreover, inactivation of esterase activity clearly decreased (p<0.05) in vitro digestibilities of dry matter and neutral detergent fiber, the total degradable fraction, and total volatile fatty acid in the rumen. Conclusion: This study concluded that FD inoculant confirmed esterase activity on rye silage harvested at dough stage, while AT inoculant could not be confirmed with antifungal activity due to the absence of mold in all silages.

Antifungal and carboxylesterase-producing bacteria applied into corn silage still affected the fermented total mixed ration

Paradhipta Dimas Hand Vidya,Seo Myeong Ji,Jeong Seung Min,주영호,Lee Seong Shin,Seong Pil-Nam,이혁준,김삼철 아세아·태평양축산학회 2023 Animal Bioscience Vol.36 No.5

Objective: This study investigated the effects of corn silage as a source of microbial inoculant containing antifungal and carboxylesterase-producing bacteria on fermentation, aerobic stability, and nutrient digestibility of fermented total mixed ration (FTMR) with different energy levels. Methods: Corn silage was used as a bacterial source by ensiling for 72 d with an inoculant mixture of Lactobacillus brevis 5M2 and L. buchneri 6M1 at a 1:1 ratio. The corn silage without or with inoculant (CON vs MIX) was mixed with the other ingredients to formulate for low and high energy diets (LOW vs HIGH) for Hanwoo steers. All diets were ensiled into 20 L mini silo (5 kg) for 40 d in quadruplicate. Results: The MIX diets had lower (p<0.05) acid detergent fiber with higher (p<0.05) in vitro digestibilities of dry matter and neutral detergent fiber compared to the CON diets. In terms of fermentation characteristics, the MIX diets had higher (p<0.05) acetate than the CON diets. The MIX diets had extended (p<0.05) lactic acid bacteria growth at 4 to 7 d of aerobic exposure and showed lower (p<0.05) yeast growth at 7 d of aerobic exposure than the CON diets. In terms of rumen fermentation, the MIX diets had higher (p<0.05) total fermentable fraction and total volatile fatty acid, with lower (p<0.05) pH than those of CON diets. The interaction (p = 0.036) between inoculant and diet level was only found in the immediately fermentable fraction, which inoculant was only effective on LOW diets. Conclusion: Application of corn silage with inoculant on FTMR presented an antifungal effect by inhibiting yeast at aerobic exposure and a carboxylesterase effect by improving nutrient digestibility. It also indicated that fermented feedstuffs could be used as microbial source for FTMR. Generally, the interaction between inoculant and diet level had less effect on this FTMR study.

왜가리(Ardea cinerea)의 월동생태에 관한 연구

한성우(Sung-Woo Hand),함규황(Hahm Kyu-Hwang) 한국조류학회II 2000 한국조류학회지 Vol.7 No.2

The Reference Genetic Linkage Map for the Multinational Brassica rapa Genome Sequencing Project

Yong Pyo Lim,Su Ryun Choi,Graham R. Teakle,Prikshit Plaha,Jeong Hee Kim,Charlotte J. Allender,Elena Beynon,Zhong Yun. Piao,Tae Ho Han,Graham J. King,Guy C. Barker,Paul Hand Hand,Derek J. Lydiate,Jacqu 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Understanding, Current Status, and Future Directions for Epistemic Practices in STEM+CS Education

( Kyong Mi Choi ),( Brian Hand ) 한국수학교육학회 2021 수학교육연구 Vol.24 No.3

Temperature and microbial changes of corn silage during aerobic exposure

이성신,이혁준,Dimas Hand Vidya Paradhipta,주영호,김상범,김동현,김삼철 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.7

Objective: This study was conducted to estimate the temperature and microbial changes of corn silages during aerobic exposure. Methods: Kwangpyeongok (KW) and Pioneer 1543 (PI) corn hybrids were harvested at 29.7% of dry matter and chopped to 3 to 5 cm lengths. Homo (Lactobacillus plantarum; LP) or hetero (Lactobacillus buchneri; LB) fermentative inoculants at 1.2×105 colony forming unit/g of fresh forage was applied to the chopped corn forage which was then ensiled in quadruplicate with a 2×2 (hybrid×inoculant) treatment arrangement for 100 days. After the silo was opened, silage was sub-sampled for analysis of chemical compositions, in vitro digestibility, and fermentation indices. The fresh silage was continued to determine aerobic exposure qualities by recorded temperature and microbial changes. Results: The KW silages had higher (p<0.01) in vitro digestibilities of dry matter and neutral detergent fiber than those of PI silages. Silages applied with LB had higher (p<0.001) acetate concentration, but lower (p<0.01) lactate concentration and lactate to acetate ratio than those of LP silages. The interaction effect among hybrid and inoculant was detected in acetate production (p = 0.008), aerobic stability (p = 0.006), and lactic acid bacteria count (p = 0.048). The yeast was lower (p = 0.018) in LB silages than that in LP silages. During the aerobic exposure, PI silages showed higher (p<0.05) temperature and mold than KW silages, while LP silages had higher (p<0.05) lactic acid bacteria and yeast than LB silages. Conclusion: The results indicated that the changes of silage temperature during aerobic exposure seems mainly affected by mold growth, while applied LB only enhanced aerobic stability of PI silages.

베일러 챔버의 압력과 이탈리안 라이그라스의 수분함량이 원형베일 사일리지 품질에 미치는 영향

민창우,칸인앰,노현탁,Dimas Hand Vidya Paradhipta,주영호,김동언,허우영,이병현 경상국립대학교 농업생명과학연구원 2020 농업생명과학연구 Vol.54 No.5

The objectives of the present study is to evaluate the effect of baler chamber pressure and moisture concentration of Italian ryegrass on the quality of round bale silage. Italian ryegrass was wilted for 1 (High-moisture), 2 (Medium-moisture), and 4 days (Low-moisture). And then, all forages were ensiled for 60 days into round bale silo with different levels of baler chamber pressure at 115, 130, and 145 bar, respectively. Bale weights at fresh and dry were higher in high-moisture and low-moisture forages, respectively. Although there were no significant differences among the baler chamber pressure on fresh weight, their dry weights were increased. Baler chamber pressure and moisture concentration did not affect NDF, ADF, CF and CP of silage. However, the relative feed value (RFV) was highest in High-moisture bale silage with low baler chamber pressure. Lactic acid concentration was highest in High-moisture bale silage with high baler chamber pressure, but lowest in Low-moisture bale silage with medium baler chamber pressure. Butyric acid concentration was not affected by the baler chamber pressure, but decreased by increase of the pre-wilting day. Silage pH was lower in High-moisture than in Medium and Low-moistrue, but it was not affected by the baler chamber pressure. These results indicated that the moisture concentration of forage and baler chamber pressure are one of the major factors affecting on fermentation quality of Italian ryegrass round bale silage. 본 연구는 이탈리안 라이그라스 원형베일 사일리지 조제에 있어서 원료작물의 수분함량과 베일러 챔버의 압력에 따른 사일리지의 사료가치와발효품질의 변화를 구명하기 위하여 수행되었다. 수확 후 1일(고수분), 2일(중수분) 및 4일간(저수분) 각각 예건하여 서로 다른 수분함량의 이탈리안라이그라스를 베일러 챔버 압력이 115, 130 및 145 bar로 설정된 베일러로 원형베일 사일리지를 조제하여 60일간 저장하였다. 베일 사일리지의무게는 고수분 처리구에서 높게 나타났고, 건물중은 저수분 처리구가 유의적으로 높게 나타났다. 베일러 압력에 따른 사일리지의 무게는 유의적인차이는 없었으나, 압력이 증가할수록 건물중은 증가하였다. 수분함량과 베일러 압력에 따른 사일리지의 NDF, ADF, CP 및 CF는 처리 간에 유의적인차이가 없었으며, RFV (relative feed value)는 고수분 저압력 처리구에서 가장 높게 나타났다. Lactic acid는 고수분-고압력 처리구가 가장 높았으며, 저수분-중압력 처리구에서 가장 낮게 나타내었다. Butyric acid는 베일러 압력에 따른 유의적인 차이는 없었으나, 예건기간이 길어질수록 감소하였다. 사일리지 pH는 고수분 처리구가 중수분과 저수분 처리구에 비해 낮게 나타났으나, 베일러 압력에 따른 차이는 없었다. 이러한 결과는 이탈리안 라이그라스 원형베일 사일리지 조제 시 조사료의 수분함량과 베일러 챔버의 압력이 사일리지의 발효품질에 영향을 미치는 중요한 요인임을 나타내는 것이다.

논의를 강조한 탐구학습법에 따른 통합학급 초등학생의 단어 활용, 독해 및 쓰기 능력

박영근,윤세열,Brian Hand,William Therrien,Mack Shelley 한국특수교육학회 2013 특수교육학연구 Vol.48 No.2

The purpose of this study is to investigate the effects of argument-based inquiry approach for improving the literacy of students with special needs, and to introduce the Science Writing Heuristic as an argument-based inquiry approach. This study focused on how this approach influenced students' literacy such as vocabulary usage, reading comprehension, and writing skills. From the forty-eight public elementary schools in the Midwest of the U.S., twenty-four elementary schools were designated as the treatment group and the remaining as the control group. Students in the treatment group were immersed in a learning environment where there is strong emphasis on argumentation while those in the control group learned science in a traditional manner. Students' achievement levels were determined based on the mean scores of the state-standardized test in the Midwest. The findings indicated that the argument-based inquiry approach contributed to students' test performances on vocabulary usage, reading comprehension, and writing skills significantly better than the traditional approach. The argument-based inquiry approach emphasized dialogical interactions between students with special needs and students' active engagement in scientific inquiry. This approach provides meaningful learning experience through argumentation in science class, and would contribute to literacy education for students with special needs in Korean elementary school settings. 본 연구의 목적은 ‘논의를 강조한 탐구 학습법’이 초등학교 특수교육대상 학생들의 단어 활용, 독해, 쓰기능력에 미치는 효과를 분석하는데 있다. ‘논의를 강조한 탐구 학습법’ 은 특수교육 지원 요구를 가진 학생들이 또래와의 상호작용을 통하여 능동적으로 과학 탐구 활동에 참여할 수 있도록 돕는다. 본 연구의 목적을 위해서 미국 중부의 48개 일반 초등학교를 탐구적 과학 글쓰기 활동을 실시한 24개의 실험집단 학교와, 이 중재학습을 실시하지 않은 24개의 통제집단 학교로 나누고, 각 학교에서 통합교육을 받고 있는 특수교육대상 학생들을 대상으로 단어 활용, 독해, 쓰기능력 검사를 실시하였다. 연구결과, 논의를 강조한 탐구 학습법을 경험한 특수교육대상 학생들이 통제집단의 학생들보다 단어 활용, 독해, 쓰기 능력에 향상을 보인다. 우리나라 초등학교 ‘과학’ 수업 시간을 통해 논의를 강조한 탐구학습법을 활용하는 것이 특수교육지원 요구를 가진 학생들의 단어 활용, 독해, 쓰기능력 향상에 도움을 줄 수 있을 것이라고 기대한다.