High expression of keratin 6C is associated with poor prognosis and accelerates cancer proliferation and migration by modulating epithelial–mesenchymal transition in lung adenocarcinoma

Hai‑Bo Hu,Xiao‑Ping Yang,Pei‑Xia Zhou,Xin‑Ai Yang,Bin Yin 한국유전학회 2020 Genes & Genomics Vol.42 No.2

Background Lung adenocarcinoma (LUAD) is a more frequent subtype of lung cancer and most cases are discovered in the late stages. The proliferation and metastasis of LUAD are pivotal for disease progression. Despite unremitting deeper understanding of LUAD biology, the mechanisms involved in the proliferation and metastasis of LUAD remain unclear. The objective of our article was to inquiry the expression and the function of keratin 6C (KRT6C) in LUAD cells. Methods First, the expression level and prognostic value of KRT6C in LUAD tissues were analyzed on the basis of the data acquired from TCGA database. Through qRT-PCR, the expression level of KRT6C on LUAD cell lines (A549, H1299, PC-9) and human normal lung cell line MRC-5 was tested. After that, CCK8 and colony formation assays was utilized to detect cell proliferation. In addition, to explore the influence of KRT6C on LUAD migration and invasion ability, scratch wound healing and transwell assays were utilized. Through western blotting, the protein expression levels of KRT6C, PCNA, E-cadherin, N-cadherin, Snail and Vimentin were detected. Results The outcomes revealed that KRT6C was highly expressed in LUAD tissues and cell lines. Besides, elevated level of KRT6C was related to worse prognosis in LUAD patients. Ablation of KRT6C restrained proliferation, migration and invasion of A549 cells. KRT6C deficiency augmented the expression of E-cadherin as well as reduced the expression of N-cadherin, Snail and Vimentin. Conclusion Above all, these consequences indicated that depletion of KRT6C suppressed A549 cell proliferation, migration and invasion, which might be achieved by regulating EMT. In general, KRT6C is identified as a potential therapeutic target for LUAD.

출력 피이드백 제어 시스템의 적응 관측기-제어기 설계에 관한 연구

양해원 漢陽大學校 工學技術硏究所 1999 工學技術論文集 Vol.8 No.1

본 논문에서는 비모형화 특성과 외란이 존재하는 비선형 시스템에 대해 적응 백스테핑 기법을 이용해 강인한 적응 제어기를 설계한다. 특히, 제어기와 식별기 설계에 있어서는 tuning function과 modular기법을 적용한 백스테핑 기법을 사용한다. Tuning function 기법을 사용하면 overparameterization이 사라지게 된다. Modular 기법에서는 식별기 설계시 관측 오차의 유계성을 보장하는 Observer-Based Scheme과 Swapping-Based Scheme에 기초를 두고 있다 적응칙을 설계하기 위해 Switching- σ modification을 사용하고 비선형 시스템에 대한 비모형화 특성과 외란의 영향을 줄이기 위해서 제어칙을 수정한다. In this paper, we present a robust adaptive backstepping output feedback controller for nonlinear systems perturbed by unmodelled dynamics and disturbances. Especially, backstepping technique with tuning function and modular approach is used to design controller and identifier. The tuning function design procedure eliminates overparameterization. In modular design, the design of identifier is based on the observer-based scheme which possesses a strict passivity property of observer error system and on the swapping-based scheme. We use Switching- σ modification" at the update law and the modify control law to attenuate the effects of unmodelled dynamics and disturbances for nonlinear systems.

백스테핑 방법을 이용한 TORA시스템 추종제어기 설계

양해원 漢陽大學校 工學技術硏究所 2000 工學技術論文集 Vol.9 No.1

본 논문에서는 TORA 시스템에 대한 안정도를 확인하고 백스테핑 방법을 이용해 제어기를설계하였다. 제어기를 설계하는 방법은 임의의 기준 입력을 통하여 안정화 함수를 구현하고, 이를 통한 가상입력을 순차적으로 구하였다. 이러한 가상 입력을 통하여 리아프노프 안정도를 만족시키는 백스테핑 제어기를 설계하고 이 제어기를 이용해 카트의 위치와 질량체의 위치각을 추종하였다. 또 제어기를 설계하는 방법에 있어 리아프노프 함수에 기존의 지수함수 대신 삼각함수를 포함시켜 시스템이 갖게 되는 와인딩 문제를 해결하였다. 이런 방법으로 설계된 제어기와 오차변수와 안정화함수를 다르게 선택해서 설계한 제어기를 갖고 모의실험에서 각 제어기의 응답을 비교하여 백스테핑 기법으로 같은 응답을 갖는 다른 제어기를 설계할 수 있음을 보였다. In this paper, we use the backstepping method to design active controllers for tracking the TORA system. This problem is much more challenging than stabilization. We show that the control effort of the closed-loop system can be significantly improved by exploiting the backstepping design. We track the position of the cart and the angle of the mass using the controller designed by the proposed backstepping method. Thus, to solve the winding problem, we exchange a quadratic term into a trigonometric term in the Lyapunov function. We can see the flexibility of the backstepping by means of the controller designed by using the different error variable and stabilizing function.

3-Phenyl-1-(2-furyl)propenone의 반응에 관한 이론적 연구

박해윤,맹주양 순천향대학교 1994 논문집 Vol.17 No.3

3-Phenyl-l-(2-furyl) propenone Was prepared by the condensation reactions of acetylfuran with benzaldehyde. The reactivity of 3-phenyl-l- (2-furyl) propenone has been studied by CNDO calculation for electrostatic potential energy surface, electric charge, geometric parameters in the molecular system. The calculated electric charge of b-carbon of 3-phenyl-l-(2-furyl) propenone is positive (0.055). The change in the electronic spectra of 3-phenyl-l- (2-furyl) propenone appear isosbestic point for hydrolysis. The nucleophilic adduct which was prepared by the reaction of 3-phenyl-l-(2-furyl) propenone was 3-phenyl-l- (2-furyl) -3-thiophenylpropanone. From the results, the reaction mechanism was proposed 1,4-Michael type addition reaction of 3-phenyl-l-(2-furyl) propenone with nucleophile.

Beef Meat의 조리법에 따른 sauce가 미치는 영향

남현근,양해석 광주보건대학 1999 論文集 Vol.24 No.-

In order to figure out of Meat Beef cooking condition, Grilled Meat Beef method and Roast Meat Beef method were selected. Through those methods, Roasting in the oven was better than grilling method. The hardness and softness of Meat Beef was satisfied with taste and color. The sauce is very important on the Meat Beaf cooking, because of controlling acidity for meat.

비모형화 특성 및 외란을 갖는 비선형 시스템에 대한 강인한 적응 제어기 설계에 관한 연구

현근호,양해원 漢陽大學校 工學技術硏究所 1998 工學技術論文集 Vol.7 No.1

In this paper we present a robust adaptive backstepping output-feedback controller for nonlinear systems perturbed by unmodelled dynamics and disturbances. The adaptive backstepping controller has been extremely successful in the last several years for nominal nonlinear and linear systems. However, the systems perturbed by unmodelled dynamics and disturbances have not been well studied in case of the adaptive backstepping controller. We will use "Switching-σ Modification" in the update law which has been used in the conventional robust adaptive controller and a modified control law to attenuate the effects of unmodelled dynamics and disturbances for nonlinear systems. The plant dynamics that ale represented by an output-feedback form will be transformed to the adaptive observer form by the recently developed Marino-Tomei (MT) filter. We analyze convergence, stability and transient characteristics of the resulting closed-loop system. The feasibility of the proposed controller and its performance are demonstrated by some suitable simulations.

척수압박으로 발현된 급성 골수성 백혈병에 동반된 과립구성 육종 1 례

윤상준,김영곤,김희종,박유환,정춘해,김양수,김태균,박영진,전호종 조선대학교 부설 의학연구소 2000 The Medical Journal of Chosun University Vol.25 No.1

저자들은 척수압박으로 하지마비를 보인 환자에서 수술 후에 급성 백혈병에 동반된 과립구성 육종으로 진단된 환자 1례를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Granulocytic sarcoma(GS) is an uncommon tumor composed of immature cells of the granulocytic series. Most case of GS occur in the course of acute leukemia and the blast crisis of chronic leukemia, Rarely, however, it may present before leukemia becomes clinically apparent. It may also occur in patients with myeloproliferative disoders. GS has been reported that it occurs in 3% to 9% of patients with acute myelogenous leukemia(AML) and the incidence of GS is reported to be higher in patients with t(8;21). GS occurs relatively commonly in africa and has been reported to affect 10-25% of black children presenting with AML. This is very rare case of granulocytic sarcoma with AML(FAB M5) presented with spinal cord compression which was supported by decompression laminectomy.

Direct Palladium-Catalyzed C-4 Arylation of Tri-substituted Furans with Aryl Chlorides: An Efficient Access to Heteroaromatics

Hai Yang,Zhishuo Zheng,Jian Zeng,Huajie Liu,Bing Yi 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.8

A series of functionalized furans were synthesized by way of a palladium -catalyzed coupling reaction of 2,3,5- trisubstituted furans with aryl chlorides through C-H bond cleavages at C-4 position. The feature of the reaction was facilitative preparation of furan derivatives with good functional group tolerance. All reactions gave the desired products in moderate to good yields in the presences of BuAd2P and t-BuOK in DMF at 120 oC after 15 h.

Crack identification based on Kriging surrogate model

Hai-yang Gao,Xing-lin Guo,Xiao-fei Hu 국제구조공학회 2012 Structural Engineering and Mechanics, An Int'l Jou Vol.41 No.1

Kriging surrogate model provides explicit functions to represent the relationships between the inputs and outputs of a linear or nonlinear system, which is a desirable advantage for response estimation and parameter identification in structural design and model updating problem. However, little research has been carried out in applying Kriging model to crack identification. In this work, a scheme for crack identification based on a Kriging surrogate model is proposed. A modified rectangular grid (MRG) is introduced to move some sample points lying on the boundary into the internal design region, which will provide more useful information for the construction of Kriging model. The initial Kriging model is then constructed by samples of varying crack parameters (locations and sizes) and their corresponding modal frequencies. For identifying crack parameters, a robust stochastic particle swarm optimization (SPSO) algorithm is used to find the global optimal solution beyond the constructed Kriging model. To improve the accuracy of surrogate model, the finite element (FE) analysis soft ANSYS is employed to deal with the re-meshing problem during surrogate model updating. Specially, a simple method for crack number identification is proposed by finding the maximum probability factor. Finally, numerical simulations and experimental research are performed to assess the effectiveness and noise immunity of this proposed scheme.

The Expression of Carnosine and Its Effect on the Antioxidant Capacity of Longissimus dorsi Muscle in Finishing Pigs Exposed to Constant Heat Stress

Yang, Peige,Hao, Yue,Feng, Jinghai,Lin, Hai,Feng, Yuejin,Wu, Xin,Yang, Xin,Gu, Xianhong Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.12

The objective of this study was to assess the effects of constant high ambient temperatures on meat quality, antioxidant capacity, and carnosine expression in longissimus dorsi muscle of finishing pigs. Castrated 24 male DLY (crossbreeds between Landrace${\times}$Yorkshire sows and Duroc boars) pigs were allocated to one of three treatments: constant ambient temperature at $22^{\circ}C$ and ad libitum feeding (CON, n = 8); constant high ambient temperature at $30^{\circ}C$ and ad libitum feeding (H30, n = 8); and constant ambient temperature at $22^{\circ}C$ and pair-fed with H30 (PF, n = 8). Meat quality, malondialdehyde (MDA) content, antioxidant capacity, carnosine content, and carnosine synthetase (CARNS1) mRNA expression in longissimus dorsi muscle were measured after three weeks. The results revealed that H30 had lower $pH_{24h}$, redness at 45 min, and yellowness at 24 h post-mortem (p<0.05), and higher drip loss at 48 h and lightness at 24 h post-mortem (p<0.01). Constant heat stress disrupted the pro-oxidant/antioxidant balance in longissimus dorsi muscle with higher MDA content (p<0.01) and lower antioxidant capacity (p<0.01). Carnosine content and CARNS1 mRNA expression in longissimus dorsi muscle of H30 pigs were significantly decreased (p<0.01) after three weeks at $30^{\circ}C$. In conclusion, constant high ambient temperatures affect meat quality and antioxidant capacity negatively, and the reduction of muscle carnosine content is one of the probable reasons.