MiR-323-5p acts as a Tumor Suppressor by Targeting the Insulin-like Growth Factor 1 Receptor in Human Glioma Cells

Lian, Hai-Wei,Zhou, Yun,Jian, Zhi-Hong,Liu, Ren-Zhong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.23

Background: MicroRNAs, small noncoding RNA molecules, can regulate mammalian cell growth, apoptosis and differentiation by controlling the expression of target genes. The aim of this study was to investigate the function of miR-323-5p in the glioma cell line, U251. Materials and Methods: After over-expression of miR-323-5p using miR-323-5p mimics, cell growth, apoptosis and migration were tested by MTT, flow cytometry and cell wound healing assay, respectively. We also assessed the influence of miR-323-5p on the mRNA expression of IGF-1R by quantitative real-time reverse transcriptase PCR (qRT-PCR), and on the protein levels by Western blot analysi. In addition, dual-luciferase reporter assays were performed to determine the target site of miR-323-5p to IGF-1R 3'UTR. Results: Our findings showed that over-expression of miR-323-5p could promote apoptosis of U251 and inhibit the proliferation and migration of the glioma cells. Conclusions: This study demonstrated that increased expression of miR-323-5p might be related to glioma progression, which indicates a potential role of miR-323-5p for clinical therapy.

Synthesis and Structure Analysis of α and β Forms of [12] Metallacrown-6 Nickel(II) Complex: [Ni6(SCH2CH2CH3)12]

Hai Lian Xiao,Fang Fang Jian,Ke Jie Zhang 대한화학회 2009 Bulletin of the Korean Chemical Society Vol.30 No.4

Two modifications of the α and β forms of propyl mercaptan nickel(II) cluster, [Ni6(SCH2CH2CH3)12], have been synthesized and their crystal structures have been determined by single-crystal X-ray diffraction. The alkyl groups are away from Ni6 ring in α form whereas they are near to the Ni atom in β form. The distance of Ni-H in β form [2.576(5) Å] is much shorter than that in α form [3.101(2) Å]. In the crystal lattice of β form, the whole structure forms a flower shape.

Converged Mobile Cellular Networks and Wireless Sensor Networks for Machine-to-Machine Communications

( Lian Hai Shan ),( Zhen Hong Li ),( Hong Lin Hu ) 한국인터넷정보학회 2012 KSII Transactions on Internet and Information Syst Vol.6 No.1

In recent years, machine-to-machine (M2M) communications are under rapid development to meet the fast-increasing requirements of multi-type wireless services and applications. In order to satisfy M2M communications requirements, heterogeneous networks convergence appears in many areas, i.e., mobile cellular networks (MCNs) and wireless sensor networks (WSNs) are evolving from heterogeneous to converged. In this paper, we introduce the system architecture and application requirement for converged MCN and WSN, where mobile terminals in MCN are acting as both sensor nodes and gateways for WSN. And then, we discuss the joint optimization of converged networks for M2M communications. Finally, we discuss the technical challenges in the converged process of MCN and WSN.

Effects of Preemptive Analgesia with Parecoxib Sodium on Haemodynamics and Plasma Stress Hormones in Surgical Patients with Thyroid Carcinoma

Wang, Lian-Dong,Gao, Xia,Li, Jun-Ying,Yu, Hong-Yan,Su, Hai-Wen,Liu, Lian-Zhong,Qi, Jun Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.9

Background: To investigate the effects of preemptive analgesia with parecoxib sodium on haemodynamics and plasma stress hormones in surgical patients with thyroid carcinoma. Materials and Methods: Fifty-seven patients with thyroid carcinoma who underwent thyroidectomy selectively in Laiwu Hospital Affiliated to Taishan Medical University and Binzhou People's Hospital were selected and randomly divided into three groups, 19 cases in each group. The control group was intravenously injected 0.9% sodium chloride injection before anesthesia induction; trial group I was intravenously injected with parecoxib sodium 20 min before anesthesia induction; based on trial group I, trial group II was injected with parecoxib sodium again 12 h after surgery. The levels of plasma norepinephrine (NE), cortisol (Cor) and blood glucose before, 12 and 24 h after surgery and changes of haemodynamics before surgery, at the end of surgery and 12, 24 and 48 h after surgery were compared in the three groups. Besides, visual analogue scale (VAS) scores were recorded 4, 8, 12 and 24 h after surgery. Results: 12 and 24 h after surgery, the levels of plasma NE and Cor in three groups rose dramatically (P<0.05 or (P<0.01); The levels of plasma NE and Cor in trial groups I and II were evidently lower than in control group (P<0.05 or P<0.01), and those in trial group II slightly lower than in trial group I. 12 h after surgery, the heart rates (HR) and systolic pressures (SBP) in trial groups I and II increased obviously by comparison to surgery before (P<0.05 or P<0.01), but gradually returned to the preoperative level. HR, SBP and diastolic pressures (DBP) in trial groups I and II at the end of surgery and 12 h after surgery were all lower than in the control group, and significant differences were present (P<0.05 or (P<0.01). At 4, 8, 12 and 24 h after surgery, VAS scores in trial groups I and II were markedly lower than in the control group (P<0.05 or (P<0.01), the scores in trial group II being the lowest. Conclusions: Combined application of parecoxib sodium for preemptive analgesia before anesthesia and after surgery can effectively reduce the levels of plasma stress hormones and improve analgesic effects in surgical patients with thyroid carcinoma, and without conspicuous impact on haemodynamics.

Hydraulic fracturing experiments of highly deviated well with oriented perforation technique

Zhu, Hai Y.,Deng, Jin G.,Liu, Shu J.,Wen, Min,Peng, Cheng Y.,Li, Ji R.,Chen, Zi J.,Hu, Lian B.,Lin, Hai,Guang, Dong Techno-Press 2014 Geomechanics & engineering Vol.6 No.2

In order to investigate the effect of different perforation angles (the angle between the perforation direction and the maximum horizontal principal stress) on the fracture initiation and propagation during hydraulic fracturing of highly deviated well in oil & gas saturated formation, laboratory experiments of the hydraulic fracturing had been carried out on the basis of non-dimensional similar criteria by using 400^3 $mm^3$ cement cubes. A plane fracture can be produced when the perforations are placed in the direction of the maximum horizontal principal stress. When the perforation angle is $45^{\circ}$, the fractures firstly initiate from the perforations at the upper side of the wellbore, and then turn to the maximum horizontal principal stress direction. When the well deviation angle and perforation angle are both between $45^{\circ}$ and $90^{\circ}$, the fractures hardly initiate from the perforations at the lower side of the wellbore. Well azimuth (the angle between the wellbore axis and the maximum horizontal principal stress) has a little influence on the fracture geometries; however it mainly increases the fracture roughness, fracture continuity and the number of secondary fractures, and also increases the fracture initiation and propagation pressure. Oriented perforating technology should be applied in highly deviated well to obtain a single plane fracture. If the well deviation angle is smaller, the fractures may link up.

Synthesis and Structure Analysis of α and β Forms of [12] Metallacrown-6 Nickel(II) Complex: [Ni₆(SCH₂CH₂CH₃)₁₂]

Xiao, Hai Lian,Jian, Fang Fang,Zhang, Ke Jie Korean Chemical Society 2009 Bulletin of the Korean Chemical Society Vol.30 No.4

Two modifications of the ${\alpha}\;and\;{\beta}$ forms of propyl mercaptan nickel(II) cluster, [$Ni_6(SCH_2CH_2CH_3)_{12}$], have been synthesized and their crystal structures have been determined by single-crystal X-ray diffraction. The alkyl groups are away from $Ni_6$ ring in $\alpha$ form whereas they are near to the Ni atom in $\beta$ form. The distance of Ni-H in $\beta$ form [2.576(5) $\AA$] is much shorter than that in $\alpha$ form [3.101(2) $\AA$]. In the crystal lattice of $\beta$ form, the whole structure forms a flower shape.

Expression, Purification and Properties of Shikimate Dehydrogenase from Mycobacterium Tuberculosis

Xue Lian Zhang,Shun Bao Zhang,Fang Hao,Xu Hui Lai,Hai Dong Yu,Yi Shu Huang,Hong Hai Wang 한국생활환경학회 2005 BMB Reports Vol.38 No.5

PageRank 알고리즘 활용한 태권도 선수들의 랭킹 산정

손해련(Hai-Lian Sun),이성노(Seong-no Lee),김만근(Man-Keun Kim) 세계태권도문화학회 2023 세계태권도문화학회지 Vol.14 No.3

The purpose of this study is to apply the Olympic Kyorugi Ranking system and PageRank algorithm to the results of four world-class taekwondo competitions held from 2020 to 2023, including G20, G14, G10, and G6, to calculate the rankings. Based on the results, a comparison between the two algorithms is conducted to provide basic information for taekwondo ranking calculation methods. For the purpose of this study, information was collected from the official website of the World Taekwondo Federation. Use MS-Excel, PyCharm, and IBM SPSS (ver. 27) programs to process data. The results of this study are as follows. Firstly, using the PageRank algorithm, the gender and weight rankings of taekwondo athletes can be calculated. Secondly, using the PageRank system for ranking calculation is more suitable for considering the relative performance of players than using the Olympic Kyorugi Ranking system for ranking calculation. Thirdly, the ranking consistency calculated using the Olympic Kyorugi Ranking system and PageRank system is relatively low.

연변지역 임부의 건강행위 이행실태 및 영향요인

장해련(Zhang, Hai-Lian),이춘옥(Li, Chun-Yu),김현리(Kim, Hyunli) 한국산학기술학회 2015 한국산학기술학회논문지 Vol.16 No.11

본 연구의 목적은 임부를 대상으로 건강행위의 이행수준과 이와 관련된 영향요인을 파악하기 위함이다. 중국 연변지 역에 소재한 모자보건병원 외래를 방문한 1011명의 임부를 연구대상으로 하였고, 연구대상자들의 산전우울, 사회적지지, 자 기효능감, 건강행위 및 대상자 특성을 수집하였으며 SPSS19.0프로그램을 사용하여 기술통계, t-test, ANOVA, 피어슨 상관관 계분석 및 이분형 로지스틱회귀분석 등 통계방법으로 자료를 분석하였다. 연구결과, 임부의 건강행위 이행수준은 28.93±6.59(0~45점)인 것으로 나타났다. 임부의 건강행위에 영향을 미치는 요인으로 산전우울(OR=0.94[0.90-0.98]), 사회적 지지(OR=1.07[1.05-1.09]) 및 자기효능감(OR=1.36[1.04-1.79])으로 나타났고 대상자의 특성은 통계적으로 유의하지 않았다. 결론적으로, 임부는 중간정도로 건강행위를 이행하고 있어 이상적이지 못하였다. 따라서 임부의 건강행위 증진을 위한 중재 프로그램 개발이 시급하고 산전우울, 사회적지지 및 자기효능감에 대한 중재방안이 충분히 고려되어야 할 것이다. Purpose: To Examine the status of health behavior and identify the influencing factors of health behavior among pregnant women. Method: The participants were 1011 pregnant women who visited outpatient hospital of in Yanbian, China. Participants were interviewed using Chinese version of Edinburgh Postnatal Depression Scale(EPDS), Perceived Social Supports(PSSS), general self-efficacy, and characteristics. Descriptive statistics, t-test, ANOVA, pearson correlation coefficient, binary logistic regression were used for data analysis with SPSS 19.0. Results: The mean score of health behavior was 28.93±6.59 scores(0~45 scores). Antepartum depression(OR=0.94[0.90-0.98]), perceived social supports(OR=1.07[1.05-1.09]), self-efficacy(OR=1.36[1.04-1.79]) were predictor of health behavior among pregnant women, on the contrary to this the characteristics of pregnant women were not statistically significant. Conclusion: The implement of the health behavior among pregnant women was not ideal. It's necessary to develop a health behavior intervention focus on antepartum depression, social support, and self-efficacy for pregnant women.

Association of Cyclin D1 Variants with Head and Neck Cancer Susceptibility: Evidence from a Meta-analysis

Lin, Hai,Fang, Lian,Lin, Dong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.14

Whether cyclin D1 (CCND1) gene variants increase susceptibility to head and neck cancer (HNC) is undetermined. Therefore, we performed the present meta-analysis to systematically assess any possible association between CCND1 variants (G870A and G1722C) and HNC risk. Seventeen studies for CCND1 G870A and three studies for CCND1 G1722C were included. Overall, CCND1 polymorphisms (G870A and G1722C) had no association with increased HNC risk (p>0.05). In the subgroup analysis by smoking status, significantly increased HNC risk was found among smokers under allele contrast, homozygous comparison and recessive models (p<0.05), smoking carriers of A allele and AA genotype appearing at elevated risk. In conclusion, while there was overall a lack of any association between CCND1 polymorphisms (G870A and G1722C) and HNC risk, smokers carrying the A allele and AA genotype of the CCND1 G870A polymorphism may be susceptible to HNC development.