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      • SCIESCOPUSKCI등재
      • KCI등재

        Stiffness design and mechanical performance analysis of transverse leaf spring suspension

        Bao Zhang,Hongnan Wang,Zhi Li,Tangyun Zhang 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.3

        Suspension stiffness affects vehicle comfort and handling performance. The stiffness optimization of the transverse leaf spring suspension can be achieved by adjusting the distance between the two central installation positions of the leaf spring. This method can avoid changing the structure of the leaf spring, reduce the difficulty of product development, and shorten the product development cycle, so this type of suspension has high engineering application value. In the paper, a finite element model of the transverse leaf spring is established, and the characteristics of the stiffness, deformation and stress of the leaf spring with the distance are studied. According to the objectives of suspension dynamic deflection, body roll angle and leaf spring reliability, the distance matching design is carried out, and the design scheme is experimentally verified. The research shows that the stiffness of the leaf spring under the opposite direction loading condition is greater than that of the same direction loading condition, and the difference between the two data increases with the increase of the distance. When the distance is 800 mm, the stiffness of the suspension is 102.1 N/mm under the same loading and 220.4 N/mm under reverse loading, the maximum stress is 1487 MPa, the dynamic deflection 39.2 mm, and the maximum body roll angle is less than 6.0°. All of the above indicators meet the design requirements. The research results provide theoretical basis and reference for the design of a transverse leaf spring suspension.

      • KCI등재

        Effects of coated cysteamine hydrochloride on muscle fiber characteristics and amino acid composition of finishing pigs

        Miaomiao Bai,Hongnan Liu,Kang Xu,Rong Yu,Abimbola Oladele Oso,Jinping Deng,Yulong Yin 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.9

        Objective: This experiment was designed to determine the effects of coated cysteamine hydrochloride (CC) on muscle fiber characteristics, amino acid composition and transporters gene expression in the longissimus dorsi muscle (LDM) of finishing pigs. Methods: Two hundred and sixteen Duroc/Landrace/Yorkshire cross-bred male finishing pigs were fed with a corn-soybean basal diet supplemented with 0, 70, and 140 mg/kg cysteamine. Each group contained eight replicates of nine pigs per replicate. After 29 days, one pig was randomly selected from each replicate and slaughtered. Blood and LDM samples were collected and analyzed. Results: The results showed that supplemental dietary CC increased (p<0.05) the muscle fiber density. And CC supplementation also up-regulated (p<0.05) the expression of myosin heavy chain 1 (MyHC1) and MyHC2x mRNA levels, and down-regulated (p<0.05) MyHC2b expression in the LDM. Additionally, supplemental dietary CC reduced (p<0.05) the concentration of total cholesterol in the plasma and enhanced (p<0.05) the concentrations of essential amino acid and total amino acid in the LDM. The relative expression levels of chloramphenicol acetyltransferase 2, b0,+ amino acid transporter, and y+-L-type amino acid transporter 1 were up-regulated (p<0.05) in the LDM when pigs were fed with the dietary CC of 70 mg/kg. Conclusion: Cysteamine supplementation could increase fiber density and distribution of fiber types. It also improved the deposition of protein in the LDM by up-regulated the expression of amino acid transporters.

      • KCI등재

        Fiber-microsphere Binary Structured Composite Fibrous Membranes for Waterproof and Breathable Applications

        Jiatai Gu,Zhenzhen Quan,Liming Wang,Hongnan Zhang,Ni Wang,Xiaohong Qin,Rongwu Wang,Jianyong Yu 한국섬유공학회 2022 Fibers and polymers Vol.23 No.6

        Membranes with the functions of waterproofness and breathability are of great interest due to their huge scientificand commercial value in many practical applications. However, designing such membranes with a desirable performance bysimple and efficient fabrication process remains a tremendous challenge. Herein, novel binary structured composite fibrousmembranes were developed for waterproof and breathable applications via simple one-step multi-jet electrospinningtechnology. This composite membranes were made up of polyvinylidene fluoride (PVDF) and polyacrylonitrile (PAN). PVDF was incorporated into the PAN fiber matrix as a modifier to improve the hydrophobic behavior of fibers. Meanwhile,PVDF microspheres/fibers were employed as building blocks to orderly embed in PAN/PVDF fiber framework, impartingthe composite membranes with fluffy micro-nano hierarchical structures. Consequently, with the integrated features of robusthydrophobicity as well as elaborate fiber-microsphere binary structure, the resultant composite membranes exhibitedsynergistically modest waterproof and breathable performances with the hydrostatic pressure of 18.04 kPa, water vaportransmittance (WVT) rate of 4.65 kg m-2 d-1, and air permeability of 64.19 mm s-1. This simple preparation process andunique composite structure may provide a new insight to design and fabricate waterproof and breathable membranes forvariously potential applications, including the protective clothing, separation membrane, and filter.

      • SCISCIESCOPUS

        A Three-step Proteolytic Cascade Mediates the Activation of the Peptidoglycan-induced Toll Pathway in an Insect

        Kim, Chan-Hee,Kim, Su-Jin,Kan, Hongnan,Kwon, Hyun-Mi,Roh, Kyung-Baeg,Jiang, Rui,Yang, Yu,Park, Ji-Won,Lee, Hyeon-Hwa,Ha, Nam-Chul,Kang, Hee Jung,Nonaka, Masaru,,derhä,ll, Kenneth,Lee, Bok Lu American Society for Biochemistry and Molecular Bi 2008 The Journal of biological chemistry Vol.283 No.12

        <P>The recognition of lysine-type peptidoglycans (PG) by the PG recognition complex has been suggested to cause activation of the serine protease cascade leading to the processing of Spätzle and subsequent activation of the Toll signaling pathway. So far, two serine proteases involved in the lysine-type PG Toll signaling pathway have been identified. One is a modular serine protease functioning as an initial enzyme to be recruited into the lysine-type PG recognition complex. The other is the Drosophila Spätzle processing enzyme (SPE), a terminal enzyme that converts Spätzle pro-protein to its processed form capable of binding to the Toll receptor. However, it remains unclear how the initial PG recognition signal is transferred to Spätzle resulting in Toll pathway activation. Also, the biochemical characteristics and mechanism of action of a serine protease linking the modular serine protease and SPE have not been investigated. Here, we purified and cloned a novel upstream serine protease of SPE that we named SAE, SPE-activating enzyme, from the hemolymph of a large beetle, Tenebrio molitor larvae. This enzyme was activated by Tenebrio modular serine protease and in turn activated the Tenebrio SPE. The biochemical ordered functions of these three serine proteases were determined in vitro, suggesting that the activation of a three-step proteolytic cascade is necessary and sufficient for lysine-type PG recognition signaling. The processed Spätzle by this cascade induced antibacterial activity in vivo. These results demonstrate that the three-step proteolytic cascade linking the PG recognition complex and Spätzle processing is essential for the PG-dependent Toll signaling pathway.</P>

      • SCIESCOPUSKCI등재

        Effect of Sea Buckthorn Leaves on Inosine Monophosphate and Adenylosuccinatelyase Gene Expression in Broilers during Heat Stress

        Zhao, Wei,Chen, Xin,Yan, Changjiang,Liu, Hongnan,Zhang, Zhihong,Wang, Pengzu,Su, Jie,Li, Yao Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.1

        The trial was conducted to evaluate the effects of sea buckthorn leaves (SBL) on meat flavor in broilers during heat stress. A total 360 one-day-old Arbor Acre (AA) broilers (male) were randomly allotted to 4 treatments with 6 replicates pens pretreatment and 15 birds per pen. The control group was fed a basal diet, the experimental group I, II and III were fed the basal diet supplemented with 0.25%, 0.5%, 1% SBL, respectively. During the 4th week, broilers were exposed to heat stress conditions ($36{\pm}2^{\circ}C$), after which, muscle and liver samples were collected. High performance liquid chromatography (HPLC) was performed to measure the content of inosine monophosphate (IMP); Real-Time PCR was performed to determine the expression of the ADSL gene. The results showed that the content of breast muscle IMP of group I, II and III was significantly increased 68%, 102% and 103% (p<0.01) compared with the control, respectively; the content of thigh muscle IMP of group II and III was significantly increased 56% and 58% (p<0.01), respectively. Additionally, ADSL mRNA expression in group I, II and III was increased significantly 80%, 65% and 49% (p<0.01) compared with the control, respectively. The content of IMP and expression of ADSL mRNA were increased by basal diet supplemented with SBL, therefore, the decrease of meat flavor caused by heat stress was relieved.

      • Deformation estimation of plane-curved structures using the NURBS-based inverse finite element method

        Runzhou You,Liang Ren,Tinghua Yi,Hongnan Li 국제구조공학회 2023 Structural Engineering and Mechanics, An Int'l Jou Vol.88 No.1

        An accurate and highly efficient inverse element labelled iPCB is developed based on the inverse finite element method (iFEM) for real-time shape estimation of plane-curved structures (such as arch bridges) utilizing onboard strain data. This inverse problem, named shape sensing, is vital for the design of smart structures and structural health monitoring (SHM) procedures. The iPCB formulation is defined based on a least-squares variational principle that employs curved Timoshenko beam theory as its baseline. The accurate strain‒displacement relationship considering tension‒bending coupling is used to establish theoretical and measured section strains. The displacement fields of the isoparametric element iPCB are interpolated utilizing nonuniform rational B-spline (NURBS) basis functions, enabling exact geometric modelling even with a very coarse mesh density. The present formulation is completely free from membrane and shear locking. Numerical validation examples for different curved structures subjected to different loading conditions have been performed and have demonstrated the excellent prediction capability of iPCBs. The present formulation has also been shown to be practical and robust since relatively accurate predictions can be obtained even omitting the shear deformation contributions and considering polluted strain measures. The current element offers a promising tool for real-time shape estimation of plane-curved structures.

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