전기도금한 교정용 스테인레스스틸 선재의 세포독성에 관한 연구

이계형,황현식,이옥,성영은 전남대학교 치의학연구소 2003 구강과학 Vol.15 No.1

The purpose of this study was to evaluate the possible clinical application through examining the cytotoxicity of orthodontic wire, which was increased diameter using electroplating. First, nickel plating was done on the commercially available stainless steel wire using a electroplating technique. And for the comparison electroplated wire with ready made stainless steel wire and titanium or copper. each wire was incubates for 72 hours on the medium. The release of the metal ion was measured using ICP-AES. The incubated medium was diluted 75%, 50%, 25% and added on the microplate each with Balb/c 373 mouse fibroblast for MTT analysis and compared with medium only. Through the measuring of the change in absorbency value of each wire group and the difference of absorbency value according to change of dilution, following results were obtained. 1. The results of ICP-AES analysis showed that lots of nickel ions were isolated from electroplated orthodontic wires and lots of copper ions were isolated from copper. 2. The inverted microscope showed that numbers of cells were decreased at the electroplated wire and copper. 3. The results of MTT analysis showed that there was no difference of the absorbency value of titanium at any dilution. 4. The results of MTT analysis showed that the absorbency values of the electroplated wires were lower than those of medium only at all dilutions, and showed statistically significant differences(p<0.001). 5. The results of MTT analysis showed that the absorbency value of the stainless steel wires were lower than those of medium only at all dilutions, and showed statistically significant differences(p<0.05). 6. The results of MTT analysis showed that the absorbency value of the copper were lower than those of medium only at all dilutions, and showed statistically significant differences(p<0.001). 7. Assessment as per ISO 10993, part 5, titanium and stainless steel wire investigated corresponded to "non-cytotoxic". Whereas copper was "highly cytotoxic", electroplated wire was alloted to "moderate cytotoxic". The results of this study indicate that the electroplated orthodontic wires need additional efforts in order to decrease cytotoxicity for their clinical applications.

Snorer의 앙와위와 직립위에서의 상도의 크기

이계형,김종철 전남대학교 치과대학 1995 전남치대논문집 Vol.7 No.1

The purpose of this study was to compare and evaluate the upper airway structure between the snorers and asymptomatic control subjects depending on the positional change. Lateral cephalograms in the upright and supine position were taken in 25 female snorers and 20 female asymptomatic control subjects. The length and the area of the soft palate, tongue and airway were measured and evaluated statistically. The results obtained were as follows : 1. The snorers showed longer and higher tongue, narrower and longer airway, inferiorly positioned hyoid bone, longer and broader soft palate and narrower hypopharynx than the control subjects both in the upright and supine position. In addition, the snorers showed broader tongue area and narrower oropharynx area than the control subjects in supine position. 2. Depending on the positional change from upright to supine position, the controls and the snorers showed decreased airway length and superior positioned the hyoid bone. In addition, the snorers showed decreased tongue length and height, airway length and thickness and oropharynx area, but increased tongue area and soft palate area.

전기도금한 교정용 스테인레스스틸 선재의 세포독성에 관한 연구

이계형,조진형,이기헌,황현식 대한치과교정학회 2005 대한치과교정학회지 Vol.35 No.2

본 연구는 전기도금을 이용하여 교정용 스테인레스스틸 선재의 직경을 증가시킨 후 세포독성을 알아봄으로써 이의 임상적 적용 가능성을 알아보고자 시행되었다. 시판되고있는 교정용 스테인레스스틸 선재에 전기도금 방법을 이용하여 니켈을 도금한 후, 이를 도금하지 않은 교정용 스테인레스스틸 선재 그리고 티타늄 또는 구리와 비교하기 위하여 이들을 배지에 넣고 72시간 배양하였다. 이 배양액을 금속 유리량 측정을 위해 ICP-AES(inductively coupled plasma atomic emission spectrophotometer) 분석을 시행한 결과 전기도금한 선재에서 많은 양의 니켈 이온이 유리되었으며, 구리에서는 많은 양의 구리 이온이 유리되었다. Microplate에 Balb/c 3T3 mouse fibroblast를 넣고 각 선재를 배양한 배양액을 75%, 50%, 25%로희석시켜 첨가한 후 MTT (3-[4.5-dimethylthiazol-2yl]-2.5-diphenyl tetrazolium bromide)분석을 시행하여 이를 배지만을 첨가한 경우와 흡광도를 비교하였다. 티타늄은 모든 농도에서 배지만을 첨가한 경우에 비해 흡광도 차이를 보이지 않았지만전기도금한 선재(p<0.001), 스테인레스스틸선재(p<0.05), 구리(p<0.001)는 배지만을 첨가한 경우보다 통계적으로 유의한 흡광도 감소를 보였다. ISO 10993, part5에 따라 구리는 "강한 독성"을 보이는 반면 티타늄과 스테인레스스틸 선재는 "무독성", 그리고 전기도금 선재는 "중등도 독성"으로 나타났다. 이상의 결과는 전기도금을 이용하여 교정용 선재의 직경을 증가시키는 방법이 임상에 적용되기 위해서는 세포독성을 줄이기 위한 추가의 연구가 필요함을 시사하였다. The purpose of this study was to examine the cytotoxicity of orthodontic wire which had an increased diameter through electroplating, and to evaluate its possoble clinical applications. First nickel plating was carried out on the commercially available stainless steel wire using an electroplating technique. For the comparison of the electroplated wire with ready made stainless steelwire and titanium or copper, each wire was incybated for 72 hours in a medium. The release of the metal ion was measured using ICP-AES(Inductively Coupled Plasma Atomic Emission Spectrophotometer). Balb/c 3T3 mouse fibroblast was put on a microplate and placed in an incubated medium of 75%, 50%, and 20% dilation. An MTT analysis was used to compare with the medium only. The changein absorbency value of each wire group and the difference of absorbency value according to the change of dilution was measured. The results of ICP-AES analysis showed that great amount nickel ion was isolated from electrpolated orthodontic wires and great amount copper ion was isolated from copper.The results of the MTT analysis showed that there was no difference in the absorbency value of titanium at any dilution. However the electroplated wires(p<0.001), the stainless steel wires(p<0.05) and the copper(p<0.001) were statistically significantly lower than those of medium only at all dilutions. Assessment as per ISO 10993, part5, showed that electroplated wire was alloted to "moderate cytotoxic", the titanium and stainless steel wire were "non-cytotoxic". The results of this study indicate that the electroplated or thodontic wires need additional efforts to dectease cytotoxicity for their clinical applications.

Visible Human Data의 3차원 가시화에 대한 연구

최형근,김동현,탁계래,신현준 건국대학교 의과학연구소 2000 건국의과학학술지 Vol.10 No.-

Visualization is the process of exploring, transforming and viewing data as images to gain understanding and insight into the data. Visualization in medicine is helpful in understanding human anatomy by presenting the information in a form that is not only pleasing but also easily recognizable as well as in gaining functional attributes of medical systems, such as biomechanical and physiological properties. Medical applications include accurate anatomy and function mapping, enhanced diagnosis. accurate treatment planning and rehearsal, and education/training. The Visible Human data set of the National Library of Medicine, the most complete anatomically detailed. computerized database of the human body ever assembled, has been used for these purposes. In this study, we have visualized the Visible Human Data set with VTK(Visualization ToolKit, Kitware Inc.), IAP(Image Application Platform, ISG Inc.) and Visual C++ 6.0. A motivation of this study is the fact that computer imaging techniques have become an important diagnostic tool in the practice of modern medicine. The 3D visualization system consists of a basic 2D image processing such as filtering, panning, zooming and measuring image as well as 3D image processing such as multiplanar reformatting, surface rendering and volume rendering. The wide-spread use and accessibility of the web have been required to provide the visualization of 3D image on the web. Thus, we have developed web-based medical 3D visualization system that supports World-Wide-Web using VRML and client/server architecture.

Snorer의 앙와위와 직립위에서의 상기도 크기

김종철,조홍규,이계형 대한치과교정학회 1996 대한치과교정학회지 Vol.26 No.1

Snorer와 정상인의 상기도 부위 연조직의 차이를 비교하며, 자세의 변화에 따른 snorer와 정상인의 상기도 부위 연조직 크기의 변화를 알아보고자 여성에서 snorer 25명과 정상군 20명을 대상으로 직립위와 앙와위에서 측모 두부 방사선 계측 사진을 촬영한 후 연구개, 혀 및 상기도에 관한 거리와 면적을 계측하고 통계학적으로 분석하여 다음과 같은 결과를 얻었다. 1. 직립위와 앙와위에서 snorer군은 정상군에 비해 혀의 길이가 길고 높이가 높았으며 기도가 좁고 길며 설골은 하방에 위치하였고 넓은 연구개와 좁은 hypopharynx를 가졌다. 앙와위에서 snorer군은 이와 더불어 정상군보다 더 넓은 혀와 더 좁은 oropharynx를 가졌다. 2. 직립위에서 앙와위로 자세의 변화에 따라 정상군과 snorer군 모두에서 기도의 길이가 감소하였고 설골이 더 상방위치 되었다. snorer군에서는 이와 더불어 혀의 길이와 높이, 기도의 두께와 oropharynx 면적은 감소하였으며 혀와 연구개 면적은 증가하였다. The purpose of this study was to compare and evaluate the upper airway structure between the snorers and asymptomatic control subjects depending on the positional change. Lateral cephalograms in the upright and supine position were taken in 25 female snorers and 20 female asymptomatic control subjects. The length and the area of the soft palate, tongue and airway were measured and evaluated statistically. The results obtained were as follows: 1. The sonorers showed longer and higher tongue, narrower and longer airway, inferiorly positioned hyoid bone, longer and broader soft palate and narrower hypopharynx than the control subjects both in the upright and supine position. In addition, the snorers showed broader tongue area and narrower oropharynx area than the control subjects in supine position. 2. Depending on the positional change from upright to supine position, the controls and the snorers showed decreased airway length and superior positioned the hyoid bone. In addition, the snorers showed decreased tongue length and height, airway length and thickness and oropharynx area, but increased tongue area and soft palate area.

[논문]지진격리교량의 내진설계를 위한 표준 인공지진파 생성

김남식,김재민,이계희,강형택 釜山大學校生産技術硏究所 2007 生産技術硏究所論文集 Vol.66 No.-

본 연구에서는 지진격리교량의 내진설계에 사용할 수 있는 인공지진파의 생성기법을 제시하였다. 또한,생성한 지진파가 적절한지 조사하였으며,지진격리교량의 내진설계기준(안)을 토대로 인공지진파 작성을 수행하였다. 설계지반응답스펙트럼을 만족시키기 위하여 22개의 가속도시간이력을 작성하였다. 얻어진 인공지진파는 (1) 통제진동수에서 인공지진파의 평균응답스펙트럼 평가, (2) EPGA(유효최대지반가속도) 평가,(3) 인공지반가속도 사이의 상관계수 평가, 동으로 적합성을 평가하였다. 최종적으로 생성된 인공지진파를 이용하여 4쌍의 지진파를 사용한 최대응답과 7쌍의 지진파를 사용한 평균 응답을 계산하여 제안된 인공지진파 시간이력의 타당성을 제시하였다.

Analysis of Parental Decisions Based on Sex Chromosome Abnormalities Detected Prenatally : A Ten-year update (2001-2010)

Gye-Hyeong An,Kyu-Hong Choi,Jae-Hyug Yang,Moon-Young Kim,Jung-Yeol Han,So-Yeon Park,Bom-Yi Lee,Da-Eun Lee,and Hyun-Mee Ryu 대한의학유전학회 2012 대한의학유전학회지 Vol.9 No.1

Purpose: The aim of this study was to analyze parental decisions regarding pregnancies in which the fetus had sex chromosome abnormalities (SCA) over a ten-year period. Materials and Methods: We collected and reviewed records from our hospital for 2001-2010 and a genetic specialist providedgenetic counseling. Results: We diagnosed 130 cases (0.71%) with SCA out of 18,376 prenatal cases from 2001 to 2010. We reviewed the records and the results of all pregnancies. We also included cases (n=84) of apparently normal anatomic fetuses to analyze the factors influencing parental decisions. We excluded 34 cases with an obvious anomaly or a presumably bad outcome and 12 cases that were not followed up. Forty-three couples (51.2%) continued their pregnancies while forty-one (48.8%) terminated them. Of 38 mosaicism cases, 21 (55.3%) were continued. Among the 20 pregnancies assisted by reproductive techniques, 15 (75%) were continued (P=0.02). More pregnancies were continued when genetic counseling was provided (61.9%) compared to cases in which it was not provided (19%) (P=0.01). Conclusion: Genetic counseling is important in providing appropriate information to parents. Establishing guidelines and protocols will help both obstetricians and parents to make informed decisions.

Outcomes of subsequent pregnancies after uterine compression suture

( Gye Hyeong An ),( You Jung Han ),( Si Won Lee ),( Jin Hoon Chung ),( Hyun Kyong Ahn ),( Jung Yeol Han ),( Hyun Mee Ryu ),( Moon Young Kim ),( Min Hyoung Kim ) 대한산부인과학회 2012 대한산부인과학회 학술대회 Vol.98 No.-

Uterine compression suture is an effective method for treatment of postpartum hemorrhage and preserving uterus. Fertiliy data after uterine compression sutures is very limited. We report the outcomes of subsequent pregnancies after uterine compression suture. From 2006 through 2011, a total of 379 pregnant women have undergone uterine compression sutures including modified B-Lynch method or multiple square sutures. Of them, 49 women conceived and re-visited our hospital for antenatal care. We excluded 8 women whose pregnancy outcomes were unknown. We analyzed outcomes of 41 women with subsequent pregnancy after uterine compression suture. There were 4 cases (8.2%) of miscarriages and one case (2.4%) of tubal pregnancy. Three women (7.3%) had a preterm delivery. A total of 30 women (73.2%) delivered at term. Pregnancies of three women are in progress Subsequent pregnancy after uterine compression suture is reassuring.

Intussusception by plant fiber in red kangaroo (Macropus rufus)

Gye-Hyeong Woo 한국실험동물학회 2019 한국실험동물학회 학술발표대회 논문집 Vol.2019 No.7

Prevention and Control of Antimicrobial Resistance and Zoonoses : Cervical Chondroid Chordoma in a Shetland Sheep Dog

( Gye Hyeong Woo ),( Eun Jung Bak ),( Young Won Lee ),( Hiroyuki Nakayama ),( Nobuo Sasaki ),( Ha Young Kim ),( Cheong Up Choi ),( Kunio Doi ) 대한인수공통전염병학회 2007 창립총회 및 학술대회 초록집 Vol.2007 No.1