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        Nutrient Amendments Influence Endophytic Colonization of Rice by Serratia marcescens IRBG500 and Herbaspirillum seropedicae Z67

        Gyaneshwar, P.,Reddy, Pallavolu M.,Ladha, Jagdish K. 한국미생물 · 생명공학회 2000 Journal of microbiology and biotechnology Vol.10 No.5

        Serratia marcescens IRBG500 and Herbaspirillum seropedicae Z67 grow endophytically in rice. The ability of these bacteria to colonize rice grown under increased nutrient availability was assessed in variety IR72 using strains marked with transposon-based gusA. The endophytic colonization was monitored via bacterial enumeration and histochemical visualization of GUS expression of bacteria in plant tissues. Rhizoplane and endophytic colonization by both bacteria was significantly inhibited in the rice plants grown in the presence of 10mM NH_4C1. In contrast, the addition of 10mM KNO_3 showed no adverse effect on colonization. Increasing the concentration of Ca^2+ to 5mM significantly reduced endophytic colonization by both bacterial strains, whereas the addition of 0.5mM Fe2+ substantially lowered the colonization of roots by S. marcescens IRBG500 but showed no effect on colonization by H. seropedicae Z67. Taken together, these findings suggest that, like in legume-rhizobial symbiosis as well as plant-pathogen interactions, nutrient status, particularly NH^+_4and Ca^2+ concentrations in the surrounding medium, plays an important role in the regulation of endophytic infection and colonization processes in rice.

      • Cervical Screening Using Visual Inspection with Acetic Acid (VIA) and Treatment with Cryotherapy in Fiji

        Fong, James,Gyaneshwar, Rajaneshwar,Lin, Sophia,Morrell, Stephen,Taylor, Richard,Brassil, Ann,Stuart, Anne,McGowan, Catherine Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.24

        The purpose of this study was to demonstrate the feasibility of VIA screening with cryotherapy and to record normative values for indicators anticipated in similar low resource settings. Women aged 30-49 years were targeted, resulting in 1961 women screened and treated at two primary health care (PHC) centres near Suva, Fiji. Recruitment was through provision of information, education and communication (IEC). Referrals to a gynaecology outpatient department (OPD) at a referral hospital occurred throughout the screening pathway. Participation was 32% (95%CI 31-33%), higher in iTaukei (Melanesians) women (34%, 95%CI 33-36) compared to Fijians of Indian descent (26%, 95%CI 24-28). Regression analysis, adjusted for confounders, indicated significantly lower participation in those of Indian descent, and age groups 35-39 and 45-49 years. Of those examined by VIA, 190 were positive with aceto-white lesions (9.9%), within the expected range of 8-15%, with minor geographic and ethnic variation. Positive VIA results were more common in the peri-urban area, and in those aged 35-39 years. Of women aged 30-49 years, 59 received cryotherapy (none of whom had significant complications), 91 were referred to OPD, two cervical carcinomas were identified and eight cervical intra-epithelial neoplasms (CIN) II-III were diagnosed. These results provide normative findings from a community-based VIA screening program for other similar low resource settings.

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