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Meng Guizhi,Duan Hongjuan,Jia Jingying,Liu Baobao,Ma Yun,Cai Xiaoyan 아세아·태평양축산학회 2024 Animal Bioscience Vol.37 No.3
Objective: It was shown that microRNAs (miRNAs) play an important role in milk protein synthesis. However, the post-transcriptional regulation of casein expression by exogenous miRNA (xeno-miRNAs) in ruminants remains unclear. This study explores the regulatory roles of alfalfa xeno-miR162 on casein synthesis in bovine mammary epithelial cells (bMECs). Methods: The effects of alfalfa xenomiR-162 and G protein subunit gamma 11 (GNG11) on proliferation and milk protein metabolism of bMECs were detected by 5-Ethynyl-2′- Deoxyuridine (EdU) staining, flow cytometry, cell counting kit-8 (CCK-8), enzyme-linked immunosorbent assay, quantitative real-time polymerase chain reaction (qRT-PCR), and Western blot. Dual-luciferase reporter assay was used to verify the targeting relationship between GNG11 and xenomiR-162. Results: Results showed that over-expression of xenomiR-162 inhibited cell proliferation but promoted apoptosis, which also up-regulated the expression of several casein coding genes, including CSN1S1, CSN1S2, and CSN3, while decreasing the expression of CSN2. Furthermore, the targeting relationship between GNG11 and xenomiR-162 was determined, and it was confirmed that GNG11 silencing also inhibited cell proliferation but promoted apoptosis and reduced the expression of casein coding genes and genes related to the mammalian target of rapamycin (mTOR) pathway. Conclusion: Alfalfa xenomiR-162 appears to regulate bMECs proliferation and milk protein synthesis via GNG11 in the mTOR pathway, suggesting that this xeno-miRNA could be harnessed to modulate CSN3 expression in dairy cows, and increase κ-casein contents in milk.
The Design of a Client-Cloud Computing Collaborative Model
Xu Guizhi 보안공학연구지원센터 2015 International Journal of Grid and Distributed Comp Vol.8 No.5
As a new computing pattern, client-cloud computing can provide limitless computing and storage capacity according to the need for different types of client devices and avoid the increasingly skeptical cloud security question. For this purpose, a client-cloud computing collaborative architecture was built. On this basis, a collaborative service model based on the cloud and centered on clients was designed, and some crucial steps of collaborative application initiated by client were analyzed. A collaborative system development, maintenance method and process centered on clients were pointed out. The client-cloud computing collaborative model proposed in this paper provided a distributional, effective, independent, unfailing and secure architecture for the collaboration, highlighted the user’s core position in collaborative activities, permitted individual users to start and manage collaborative application fast and easy from local.
miR-143 inhibits proliferation and induces apoptosis of mammary epithelial cells in dairy goat
Zhibin Ji,Guizhi Wang,Lei Hou,Zhaohua Liu,Jianmin Wang,Tianle Chao 한국통합생물학회 2016 Animal cells and systems Vol.20 No.2
MicroRNAs are a class of post-transcriptional regulators of gene expression in multicellular organisms, which play important roles in cell fate, organ morphogenesis and carcinogenesis. In the present study, we demonstrated the critical roles of miR-143 on mammary epithelial cells of dairy goat. The test results of cell count, methylthiazolyldiphenyl-tetrazolium bromide, Hoechst33342/PI (propidium iodide) and flow cytometry showed miR-143-induced G0/G1 phase arrest, inhibited proliferation and promoted apoptosis of mammary epithelial cells; the qRT-PCR test of marker genes related to cell proliferation and apoptosis, BAX and BCL-2, supported the same conclusions. Our study presents evidence that miR-143 is an important post-transcription regulator involving in mammary cells survival, and it may have a value function in mammary gland development, lactation or involution.
Identification and Function Prediction of Novel MicroRNAs in Laoshan Dairy Goats
Ji, Zhibin,Wang, Guizhi,Zhang, Chunlan,Xie, Zhijing,Liu, Zhaohua,Wang, Jianmin Asian Australasian Association of Animal Productio 2013 Animal Bioscience Vol.26 No.3
MicroRNAs are a class of endogenous small RNAs that play important roles in post-transcriptional gene regulation by directing degradation of mRNAs or facilitating repression of target gene translation. In this study, three small RNA cDNA libraries from the mammary gland tissues of Laoshan dairy goats (Capra hircus) were constructed and sequenced, individually. Through Solexa high-throughput sequencing and bioinformatics analysis, we obtained 50 presumptive novel miRNAs candidates, and 55,448 putative target genes were predicted. GO annotations and KEGG pathway analyses showed the majority of target genes were involved in various biological processes and metabolic pathways. Our results discovered more information about the regulation network between miRNAs and mRNAs and paved a foundation for the molecular genetics of mammary gland development in goats.
Zhentao Shang,Cheng Xu,Guizhi Xie,Jun Yi,Han Huang 대한기계학회 2018 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.32 No.7
Tungsten carbide cobalt coatings are widely employed for fabricating the parts used in corrosive and abrasive environments. The coatings are often deposited on substrates via high velocity oxygen fuel spraying, which are relatively hard and brittle and difficult to machine. This work systematically investigated the effect of high speed grinding conditions on the removal characteristics and machinability of WC-10Co-4Cr coatings. The wheel speed was found to have significant effect on grinding force and temperature. The subsurface damage could be considerably reduced when the grinding conditions were judiciously selected. The high speed grinding process being developed produced a satisfactory subsurface damage layer of smaller than 15 mm without spalling and large cracking that were usually observed in conventional grinding, suggesting its suitability for post-processing of the coating/substrate system.
Keli Peng,Pan Lu,Feihong Lin,Tan Jin,Wencheng Bao,Guizhi Xie,Zhentao Shang 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.6
The level and variation of energy partition ratio to grinding chips in high speed grinding of a nickel based alloy has been investigated. The ratio to grinding chips is evaluated by relating the heat partitioning at the chip formation area with the whole thermal system, which is used to evaluate the convection heat transfer coefficient (CHTC) of water based grinding fluid. High level of CHTCs of the grinding fluid have been identified at high grinding speeds, beneficial heat transfer conditions of low ratios to the workpiece can be achieved. As the burnout happens with the CHTC dropping down to zero level, the ratio to the workpiece and grinding chips would become much higher, around 30-40 % of the grinding heat can be taken away by grinding chips. For nickel based superalloys, it is possible to achieve high material removal rates with the effective cooling of the grinding zone.
Yongjun Tan,Xuedan Luo,Mingfu Mao,Dehua Shu,Wenfei Shan,Guizhi Li,Dongcai Guo 한국물리학회 2018 Current Applied Physics Vol.18 No.11
In the present study, the SrMoO4:Eu3+ phosphors has been synthesized through hydro-thermal co-precipitation method, and single factor and orthogonal experiment method was adopted to find optimal synthesis condition. It is interesting to note that hydro-thermal temperature is a prominent effect on the luminescent intensity of SrMoO4:Eu3+ red phosphor, followed by co-precipitation temperature, calcining time, and the doping amount of Eu3+. The optimal synthesis conditions were obtained: hydro-thermal temperature is 145 °C, co-precipitation temperature is 35 °C, the calcining time is 2.5 h, and the doping amount of activator Eu3+ is 25%. Subsequently, the crystalline particle size, phase composition and morphology of the synthesized phosphors were evaluated by X-ray powder diffraction (XRD) and scanning electron microscopy (SEM). The results show that these phosphors possess a scheelite-type tetragonal structure, and the particle size is about 0.2 μm. Spectroscopic investigations of the synthesized phosphors are carried out with the help of photo-luminescence excitation and emission analysis. The studies reveal that SrMoO4: Eu3+ phosphor efficiently convert radiation of 394 nm–592 and 616 nm for red light, and the luminescence intensity of SrMoO4:Eu3+ phosphors is improved. SrMoO4:Eu3+ phosphors may be a potential application for enhancing the efficiency of white LEDs.
Jingjuan Hu,Haihua Luo,Jieyan Wang,Wenli Tang,Junqi Lu,Shan Wu,Zhi Xiong,Guizhi Yang,Zhenguo Chen,Tian Lan,Hongwei Zhou,Jing Nie,Yong Jiang,Peng Chen 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-
Chronic high-salt diet-associated renal injury is a key risk factor for the development of hypertension. However, the mechanism by which salt triggers kidney damage is poorly understood. Our study investigated how high salt (HS) intake triggers early renal injury by considering the ‘gut-kidney axis’. We fed mice 2% NaCl in drinking water continuously for 8 weeks to induce early renal injury. We found that the ‘quantitative’ and ‘qualitative’ levels of the intestinal microflora were significantly altered after chronic HS feeding, which indicated the occurrence of enteric dysbiosis. In addition, intestinal immunological gene expression was impaired in mice with HS intake. Gut permeability elevation and enteric bacterial translocation into the kidney were detected after chronic HS feeding. Gut bacteria depletion by non-absorbable antibiotic administration restored HS loadinginduced gut leakiness, renal injury and systolic blood pressure elevation. The fecal microbiota from mice fed chronic HS could independently cause gut leakiness and renal injury. Our current work provides a novel insight into the mechanism of HS-induced renal injury by investigating the role of the intestine with enteric bacteria and gut permeability and clearly illustrates that chronic HS loading elicited renal injury and dysfunction that was dependent on the intestine.