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      • KCI등재

        Influence of Mist Intervals and Aeration Rate on Growth and Second Metabolite Production of Pseudostellaria heterophylla Adventitious Roots in a Siphon-mist Bioreactor

        Gui Rong Wang,Nian Min Qi 한국생물공학회 2010 Biotechnology and Bioprocess Engineering Vol.15 No.6

        Plant adventitious root culture in bioreactors is a promising alternative for the efficient production of medicinal herbs. Adventitious roots of Pseudostellaria heterophylla were induced from callus and then cultivated in a siphon-mist bioreactor. An orthogonal test established that the optimal medium for adventitious root induction was MS medium supplemented with 1.0 mg/L naphthaleneacetic acid and 2.0 mg/L 3-indolybutyric acid. Under these conditions, the average root number was more than 14 on each 1.0 cm diameter callus and the rooting rate reached 100%. The bioreactor was equipped with an integral siphon-spraying device designed to automatically supply the liquid medium. The operation parameters of the bioreactor were assessed by varying the mist interval and the aeration velocity. The mist interval was negatively related to average growth rate of the adventitious roots and positively related to saponin and polysaccharide content. A relatively high aeration rate was necessary to achieve the maximum biomass production, but the secondary metabolite production was not enhanced by increasing the aeration velocity.

      • AZ31 Mg합금 마찰용접부의 인장시험 동안 균열의 생성과 전파에 미치는 경도분포의 영향

        강정윤(Gui-Rong Zhan) 대한용접·접합학회 2021 대한용접학회 특별강연 및 학술발표대회 개요집 Vol.2021 No.11

        HCP 결정구조를 갖는 Mg AZ31 합금의 동종 맞대기 마찰교반접합부의 인장 성질은 용접조건과 더불어 집합조직분포의 영향을 받는 것으로 알려져 있다. 본 연구에서는 마찰교반용접부의 인장성질에 미치는 회전속도(1500, 1800rpm)와 접합속도(83∼200mm/min, 4조건)의 영향과 더불어 미세조직 및 경도와의 상관관계를 규명하고, 더불어 인장시험 과정에서 파단경로와 파단형태를 분석하고, 집합조직과의 상관관계를 규명하고자 하였다. 접합부 인장강도와 FSW에서 입열로 사용되는 Weld Pitch (WP, (notation speed/welding speed))와의 상관관계를 분석한 결과, 동일 WP에서 1800prpm 시편이 인장강도가 높았고, 이들 시편들의 파단 형상(A)은 모두 시편의 SZ/TMAZ 계면에서 시편 두께 방형으로 수직으로 파단되는 것이 특징이었다. 반면에 1500rpm의 접합시편은 18N/V(83mm/min)를 제외하고, 모두 SZ/TMAZ 계면에서 TMAZ/모재 계면에 따라 파단되는 형상(B)이었다. 또한 인장강도는 1500과 1800RPM 모두 WP의 증가에 따라 증가하다 15N/V 이상에서 모두 감소하였다. 인장시험 시 인장시편의 변형과정을 연속촬영 하여 관찰한 결과, 최초 균열은 모두 시편 표면의 SZ/TMAZ 계면에서 발생하고, 각각 A형은 시편 두께 수직방향으로, B형은 SZ/TMAZ 계면에 따라 전진하여 파단되었다. 한편 자동 경도기로 경도분포를 조사한 결과, 툴과 맞닿는 표면(Crown Zone : CZ)의 경도는 모든 시편에서 평균 55HV이상이었고, WP가 증가할수록 증가하다 15WP 이상에서 감소하였으며, 동일 WP 조건에서 1800PPM의 것이 경도가 높았다. 결론적으로 인장강도 및 파단형태는 경도분포와 밀접한 관계를 가지고 있다. 즉 경도가 가장 낮은 SZ/TMAZ 경계에서 균열이 생성되고 전파되므로, 이들 영역의 경도분포와 경도 값에 따라 결정된다. 최고 인장강도를 얻을 수 있는 용접조건은 아주 좁다.

      • KCI등재

        书法美育视角下义务教育阶段书法教育刍论

        천구이롱(Gui-Rong Chen) 한국서예학회 2023 서예학연구 Vol.42 No.-

        서예교육은 중국의 초등학교 의무교육에서 綱要에 해당하는 부분으로 현재 이 단계의 서예교육은 학생들의 낮은 경필력, 붓글씨 미교육 부족 등의 현실적인 문제가 있다. 본고는 현재 서예교육 현황, 경필서예교육과 붓서예교육의 공통성과 차이점, 「中小学书法教育指导纲要」및 학생들의 학습수요 측면을 다루었다. 핵심 관점은 경필서예교육과 붓서예교육이 다르다는 것이다. 경필서예는 규범, 미관, 실용을 주요 목표로 삼아야 하며, 붓서예는 미교육과 실천을 결합해야 한다. 아울러 기술을 너무 중시하고 미학에 대한 중시를 소홀히 해서는 안된다는 것을 주장하였다.이 연구를 통해 현재 의무교육 단계의 서예교육에 대한 몇 가지 아이디어를 제공할 수 있을 것이다. Calligraphy education is an important part of compulsory education. At present, there are some practical problems in calligraphy education, such as poor writing ability and insufficient aesthetic education of calligraphy.This paper mainly studies the current situation of calligraphy education, the commonality and difference between calligraphy education and calligraphy art education, the Outline of Calligraphy Education in Primary and Secondary Schools and the students' learning needs.The key point is that calligraphy art education and writing education are different. Hard pen writing should aim at standardization, beauty and practicality.The conclusion can be used as a reference for calligraphy education in the current compulsory education stage.

      • The Research on the Sustainable growth of Listed Companies in Chinese Steel Industry

        LI Gui rong,WANG Wei 한국경영교육학회 2012 한국경영교육학회 학술발표대회논문집 Vol.2012 No.6

        Steel industry is one of the important raw materials industries in China. In the Ministry of Industry and Information Technology, steel industry is included in the plan for ten big industries to be adjusted and developed, which shows its important position in the national economy. In the first 10 years of this century, continuous and extraordinary growth is a prominent feature of China's steel industry. Between the year 2000-2010, steel production has rose from 127 million tons to more than 600 million tons, an increase of about 4 times, which built a solid foundation for a big steel country and made China a veritable big country famous for steel production. At the same time, the rapid development of China's steel industry has also resulted in tremendous energy and resource consumption, which influences the industry's sustainable growth and hinder Chinato become a powerful country of iron and steel based on qualitative and quantitative standards. Extensive growth mode, merely pursuing the increase of quantity, is not desirable. It is very important for China to seek a sustainable growth pattern for steel industry. This paper studies sustainable growth of China's steel industry from a financial point of view, which will contribute to systematically analyze the sustainable growth of the listed companies in China, to identify the problems and make more evidence-based, sensible decisions in marketing, finance and manufacturing. Also, the studies can provide timely guidance for the growth strategies of other steel companies which have been listed, to be listed, and other small and medium enterprises to promote their healthy and stable developments. And it will lay a solid foundation for the sustainable development of China's steel industry and plays an important role for China in becoming a powerful country of iron and steel.Based on the theory of enterprises' sustainable development, this essay has made a comparative analysis on typical models of sustainable growth and analyzed the internal and external factors affecting listed companies' sustainable growth. Based on the development status of listed companies in China's steel industry, this essay selected 33 companies in the steel industry listed in Shanghai and Shenzhen Stock Exchange before 2006 after removed those having special conditions, such as "ST" companies whose stock exchanges were specially treated because of consecutive losses, and companies experienced a major business restructuring, etc. With the Robert Higgins' sustainable growth model, we tested the sustainable growth of the selected listed companies in the steel industry between 2006-2010 by using Wilcoxon's signed rank test. The empirical results show that the samples have not achieved sustainable growth whether as a whole or separated year by year. We also concluded that there was a increasing gap between the real growth rates and the sustainable growth rates in five years, which indicated that it was very serious for these companies to blindly pursue high-speed growth, and the financial sustainable growth in the steel industry has not got enough attentions in these companies. So it is not optimistic to achieve sustainable growth for listed companies in the steel industry.

      • KCI등재

        Impact of a Glyphosate-Tolerant Soybean Line on the Rhizobacteria, Revealed by Illumina MiSeq

        ( Gui-hua Lu ),( Yin-ling Zhu ),( Ling-ru Kong ),( Jing Cheng ),( Cheng-yi Tang ),( Xiao-mei Hua ),( Fan-fan Meng ),( Yan-jun Pang ),( Rong-wu Yang ),( Jin-liang Qi ),( Yong-hua Yang ) 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.3

        The global commercial cultivation of transgenic crops, including glyphosate-tolerant soybean, has increased widely in recent decades with potential impact on the environment. The bulk of previous studies showed different results on the effects of the release of transgenic plants on the soil microbial community, especially rhizosphere bacteria. In this study, comparative analyses of the bacterial communities in the rhizosphere soils and surrounding soils were performed between the glyphosate-tolerant soybean line NZL06-698 (or simply N698), containing a glyphosate-insensitive EPSPS gene, and its control cultivar Mengdou12 (or simply MD12), by a 16S ribosomal RNA gene (16S rDNA) amplicon sequencing-based Illumina MiSeq platform. No statistically significant difference was found in the overall alpha diversity of the rhizosphere bacterial communities, although the species richness and evenness of the bacteria increased in the rhizosphere of N698 compared with that of MD12. Some influence on phylogenetic diversity of the rhizosphere bacterial communities was found between N698 and MD12 by beta diversity analysis based on weighted UniFrac distance. Furthermore, the relative abundances of part rhizosphere bacterial phyla and genera, which included some nitrogen-fixing bacteria, were significantly different between N698 and MD12. Our present results indicate some impact of the glyphosate-tolerant soybean line N698 on the phylogenetic diversity of rhizosphere bacterial communities together with a significant difference in the relative abundances of part rhizosphere bacteria at different classification levels as compared with its control cultivar MD12, when a comparative analysis of surrounding soils between N698 and MD12 was used as a systematic contrast study.

      • Association of Risk of Gastric Cancer and Consumption of Tobacco, Alcohol and Tea in the Chinese Population

        Tong, Gui-Xian,Liang, Han,Chai, Jing,Cheng, Jing,Feng, Rui,Chen, Peng-Lai,Geng, Qing-Qing,Shen, Xing-Rong,Wang, De-Bin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.20

        This study aimed at summarizing epidemiological research findings on associations between tobacco, alcohol and tea consumption and risk of gastric cancer (GC) in the Chinese population. The review searched PubMed, Embase, China National Knowledge Infrastructure (CNKI) and China Biology Medicine (CBM) databases and reference lists of review papers for all studies published in English or Chinese languages. Information extracted, via two independent researchers, from retrieved articles included first author, year of publication, study design, sample size, source of controls and adjusted odds ratio (OR) or relative risk (RR) with the corresponding 95% confidence intervals (CIs) for each category. Statistical analyses used software STATA version 12.0. The systematic search found 89 articles containing 25,821 GC cases and 135,298 non-cases. The overall random effects in terms of pooled OR and 95%CI for tobacco, alcohol and tea consumption were 1.62 (95%CI: 1.50-1.74), 1.57 (95%CI: 1.41-1.76) and 0.67 (95%CI: 0.59-0.76) respectively; while the heterogeneity among included studies ranged from 80.1% to 87.5%. The majority of subgroup analyses revealed consistent results with the overall analyses. All three behavioral factors showed statistically significant dose-dependent effects on GC (P<0.05). The study revealed that tobacco smoking and alcohol drinking were associated with over 1/2 added risk of GC, while tea drinking conferred about 1/3 lower risk of GC in the Chinese population. However, these results should be interpreted with caution given the fact that most of the included studies were based on a retrospective design and heterogeneity among studies was relatively high.

      • SCOPUSKCI등재

        Improvement of heat and drought photosynthetic tolerance in wheat by overaccumulation of glycinebetaine

        Wang, Gui-Ping,Hui, Zhen,Li, Feng,Zhao, Mei-Rong,Zhang, Jin,Wang, Wei The Korean Society of Plant Biotechnology 2010 Plant biotechnology reports Vol.4 No.3

        Within their natural habitat, crops are often subjected to drought and heat stress, which suppress crop growth and decrease crop production. Causing overaccumulation of glycinebetaine (GB) has been used to enhance the crop yield under stress. Here, we investigated the response of wheat (Triticum aestivum L.) photosynthesis to drought, heat stress and their combination with a transgenic wheat line (T6) overaccumulating GB and its wild-type (WT) Shi4185. Drought stress (DS) was imposed by controlling irrigation until the relative water content (RWC) of the flag leaves decreased to between 78 and 82%. Heat stress (HS) was applied by exposing wheat plants to $40^{\circ}C$ for 4 h. A combination of drought and heat stress was applied by subjecting the drought-stressed plants to a heat stress as above. The results indicated that all stresses decreased photosynthesis, but the combination of drought and heat stress exacerbated the negative effects on photosynthesis more than exposure to drought or heat stress alone. Drought stress decreased the transpiration rate (Tr), stomatal conductance (Gs) and intercellular $CO_2$ concentration (Ci), while heat stress increased all of these; the deprivation of water was greater under drought stress than heat stress, but heat stress decreased the antioxidant enzyme activity to a greater extent. Overaccumulated GB could alleviate the decrease of photosynthesis caused by all stresses tested. These suggest that GB induces an increase of osmotic adjustments for drought tolerance, while its improvement of the antioxidative defense system including antioxidative enzymes and antioxidants may be more important for heat tolerance.

      • Association Between Gestational Diabetes Mellitus and Subsequent Risk of Cancer: a Systematic Review of Epidemiological Studies

        Tong, Gui-Xian,Cheng, Jing,Chai, Jing,Geng, Qing-Qing,Chen, Peng-Lai,Shen, Xin-Rong,Liang, Han,Wang, De-Bin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.10

        Purpose: This study aimed at summarizing epidemiological evidence of the association between gestational diabetes mellitus (GDM) and subsequent risk of cancer. Materials and Methods: We searched Medline, Embase, Cancer Lit and CINAHL for epidemiological studies published by February 1, 2014 examining the risk of cancer in patients with history of GDM using highly inclusive algorithms. Information about first author, year of publication, country of study, study design, cancer sites, sample sizes, attained age of subjects and methods used for determining GDM status were extracted by two researchers and Stata version 11.0 was used to perform the meta-analysis and estimate the pooled effects. Results: A total of 9 articles documented 5 cohort and 4 case-control studies containing 10,630 cancer cases and 14,608 women with a history of GDM were included in this review. Taken together, the pooled odds ratio (OR) between GDM and breast cancer risk was 1.01 (0.87-1.17); yet the same pooled ORs of case-control and cohort studies were 0.87 (0.71-1.06) and 1.25 (1.00-1.56) respectively. There are indications that GDM is strongly associated with higher risk of pancreatic cancer (HR=8.68) and hematologic malignancies (HR=4.53), but no relationships were detected between GDM and other types of cancer. Conclusions: Although GDM increases the risk of certain types of cancer, these results should be interpreted with caution becuase of some methodological flaws. The issue merits added investigation and coordinated efforts between researchers, antenatal clinics and cancer treatment and registration agencies to help attain better understanding.

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