http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Estradiol-17β를 처리한 흰쥐 자궁에 있어서 Clomiphene이 세포내 수용체, DNA 및 cAMP 농도에 미치는 영향
김곤섭,고복현 동국대학교 경주대학 1989 論文集 Vol.8 No.-
These experiments were carried out to investigate the study on the interaction of estradiol receptor and cAMP level of clomiphene in immature rat uterus. The results obtained were summarized as follow : 1. The binding affinity of clomiphene to estradiol receptor in uterine cytosol was lower than that of estradiol-17β. clomiphene inhibited the replenishment of the receptor to cystol in uterus(P<0.01). 2. DNA level in uterus was increased at 48 hours after estradiol-17β or clomiphene injection(P<0.01) 3. cAMP level in uterus was increased at 48 hours after estradiol-17β or clomiphene injection(P<0.01)
A Study on Ocular and Skin Irritation of a New Disinfectant, Clean Natural<SUP>®</SUP>
Suk Kim,Myung Sun Chun,Hee Sik Chung,Won Chul Jung,Dong Hyeok Kim,Jeong Ju Lim,Ho Yeong Shon,Gon Sup Kim,Hu-Jang Lee 한국실험동물학회 2007 Laboratory Animal Research Vol.23 No.1
This test was performed to evaluate the ocular and skin irritation of Clean Natural<SUP>®</SUP>", a new disinfectant of which main components are propolis and wood vinegar from Quercus mongolica. The results of eye irritation test were as followings; in the single application test, 0.1 ㎖ Clean Natural<SUP>®</SUP>" eyedrops and 0.9% saline as vehicle control were applied to one eye of rabbit, respectively. The reactions were examined for 7 days after treatment. Transient, Up to 2 days, eye irritation was observed in the rabbit eyes treated with the test solution. The results of skin irritation test were as following; there were no observed clinical signs, body weight changes and gross pathologic findings during experimental period. There were no observed erythema, eschar, and edema formation on intact and abraded skin. The primary irritation index (P.LL) of Clean Natural<SUP>®</SUP>" was 0 and was evaluated none irritating product. In the present study, 10% Clean Natural<SUP>®</SUP>" was evaluated as minimally irritating and none-irritating product for eye and skin, respectively.
Gon-Sup Kim, Gyeong-Eun Hong, Hyeon-Soo Park, Jin-A Kim, Arulkumar Nagappan, Jue Zhang, Sang-rim Kang, Chung-kil Won, Jae-Hyeon Cho, Eun-Hee Kim 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.4
Fraxinus rhynchophylla (Oleaceae), a deciduous tree, is known to have properties that include anti-inflammatory, convergence, febricide, antiblenophthalmia, antidiarrhea, antileukorrhea, and so forth. In addition, it has been used for traditional herbal medicine in East Asian countries, including Korea. In this study, we investigated the antioxidant and anti-inflammatory effects of Fraxinus rhynchophylla ethanol extract (FRE) in lipopolysaccharide (LPS)-induced murine macrophage Raw 264.7 cells with FRE pretreatment. We performed DPPH-assay, Western blot, and Reverse Transcription-Polymerase Chain Reaction (RT-PCR). FRE showed 85% free radical scavenging activity at concentrations of 80 μg/ml. Results of this study also showed that FRE down-regulates Cox-2 and iNOS expression in mRNA and protein level. In conclusion, crude ethanol extract of Fraxinus rhynchophylla exhibited antioxidant and anti-inflammatory activities, and it may potentially provide a valuable source of natural herbal agent to inhibit inflammation.
Gon-Sup Kim , Arulkumar Nagappan, Hyeon-Soo Park, Kwang-Il Park, Jin-A Kim, Gyeong-Eun Hong, Silvia Yumnam, Eun-Hee Kim, Won-Sup Lee, Wang-Jae Lee, Myung-Je Cho, Woo-Kon Lee, Chung-Kil Won 충북대학교 동물의학연구소 2013 Journal of Biomedical and Translational Research Vol.14 No.2
Vitamin C (ascorbic acid) is an essential nutrient of most living tissues. We established a strain of Gulo-/- mice with known deficiency, in which vitamin C intake can be controlled by diet, like humans, and investigated the differentially expressed proteins following treatments with Helicobacter pylori and diethylnitrosamine (DENA) in the liver of Gulo-/- mice using a proteomic approach. Expression of p53, 14-3-3ε and 14-3-3δ in Gulo-/- mice liver tissue was analyzed by immunohistochemistry. 2-DE maps constructed from Gulo-/- mice liver and differentially expressed proteins in liver tissue were identified by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF/MS). In Gulo-/- mice after H. Pylori infection, followed by treatment with DENA, no differences in p53, 14-3-3ε and 14-3-3δ were observed by immunohistochemistry. Proteome analyses using MALDI-TOF/MS resulted in successful identification of 12 proteins (nine proteins were up-regulated and three were down-regulated). Specifically, peroxiredoxin-6 and Alpha-1-antitrypsin 1-4 were up-regulated in liver after H. Pylori infection followed by treatment with DENA. These results indicated that oral supplementation with vitamin C led to rescue of Gulo-/- mice from vitamin deficiency, and protected the liver from H.pylori infection and/or DENA effect, and vitamin C also protected the liver against oxidative stress
Gon-Sup Kim,Oh-Sung Park,Mun-Ki Kim,Pil-Ok Koh,Jae-Haen Cho,Chung-Kil Won 한국실험동물학회 2009 Laboratory Animal Research Vol.25 No.2
This study was performed to examine the effect of 2,3,4,4',5-pentachlorobiphenyl (PCB 114) on morphological changes in the testes of rats. PCB 114 (20 mg/kg/week) in corn oil was intraperitoneally injected into adult rats for 2, 5, and 8 weeks, with injections carried out weekly. The total body weights and testicular weights were measured at 3, 6, and 9 weeks during PCB treatment, respectively. The morphological changes in the rat testes were then analyzed by light microscopy (LM) and transmission electron microscopy (TEM). The results showed that PCB 114 caused no significant change in the body weight but caused a marked increase in the testicular weights at 6 weeks. The LM results proved that the size of their seminiferous tubules slightly increased by PCB treatment, and cytoplasmic vacuolation and damaged nuclear membrane of spermatogenic cells were evident at 6 weeks in the electon microscopical examination. These results suggest that the reproductive of the adult male rats is effective to PCB 114, and that may induce degenerative changes in testicular parenchyma of adult male rats.
Kim, Gon Sup,Kim, Dong Hyeok,Lim, Jeong Ju,Lee, Jin Ju,Han, Dae Yong,Lee, Whi Min,Jung, Won Chul,Min, Won Gi,Won, Chung Gil,Rhee, Man Hee,Lee, Hu Jang,Kim, Suk Pharmaceutical Society of Japan 2008 BIOLOGICAL & PHARMACEUTICAL BULLETIN Vol.31 No.11
<P>Salmonellosis is a major bacterial zoonosis that causes a variety of disease syndromes, from self-limiting enteritis to fatal infection in animals and food-borne infection and typhoid fever in humans. Recently, the emergence of multidrug-resistant strains of <I>Salmonella</I> sp. has caused more serious problems in public health. The present study investigated the antibacterial effects of <I>Houttuynia cordata</I> water extract (HCWE) against murine salmonellosis. In RAW 264.7 cells, there was no detectable cytotoxic effect of HCWE at any concentration between 25 and 100 μg/ml after 8-h incubation. The antibacterial activity of HCWE was then examined in a <I>Salmonella enterica</I> serovar (<I>Salmonella typhimurium</I>), and was found to increase in a dose-dependent manner at concentrations from 25 to 100 μg/ml during 8-h incubation. HCWE also affected RAW 264.7 cells including morphologic change and bacterial uptake, but there was no significant difference in bacterial replication in RAW 264.7 cells. With HCWE alone, nitric oxide (NO) production by RAW 264.7 cells did not increase, but when RAW 264.7 cells were infected by <I>S. typhimurium</I>, with or without HCWE, NO production with HCWE was 2-fold higher than that without HCWE. Treatment with HCWE did not affect inducible NO synthase (iNOS) mRNA expression by RAW 264.7 cells, but when RAW 264.7 cells with HCWE were infected by <I>S. typhimurium</I>, iNOS mRNA expression was increased during 8-h incubation. Furthermore, HCWE showed virulence reduction effects in <I>S. typhimurium</I>-infected BALB/c mice. After a lethal dose of <I>S. typhimurium</I>, the mortality rate in the HCWE untreated group was 100% at 7 d, but the HCWE 25, 50, and 100 μg/ml groups survived until 11, 17, and 23 d, respectively. These data suggest that HCWE is stable and beneficial in the treatment of bacterial infection including intracellularly replicating pathogens and may solve antimicrobial misuse and overuse.</P>