http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Sergio Bárcena-Varela,Guillermo Martínez-de-Tejada,Lukas Martin,Tobias Schuerholz,Ana Gloria Gil-Royo,Satoshi Fukuoka,Torsten Goldmann,Daniel Droemann,Wilmar Correa,Thomas Gutsmann,Klaus Brandenburg,L 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-
Sepsis, which is induced by severe bacterial infections, is a major cause of death worldwide, and therapies combating the disease are urgently needed. Because many drugs have failed in clinical trials despite their efficacy in mouse models, the development of reliable animal models of sepsis is in great demand. Several studies have suggested that rabbits reflect sepsisrelated symptoms more accurately than mice. In this study, we evaluated a rabbit model of acute sepsis caused by the intravenous inoculation of Salmonella enterica. The model reproduces numerous symptoms characteristic of human sepsis including hyperlactatemia, hyperglycemia, leukopenia, hypothermia and the hyperproduction of several pro-inflammatory cytokines. Hence, it was chosen to investigate the proposed ability of Pep19-2.5—an anti-endotoxic peptide with high affinity to lipopolysaccharide and lipoprotein—to attenuate sepsis-associated pathologies in combination with an antibiotic (ceftriaxone). We demonstrate that a combination of Pep19-2.5 and ceftriaxone administered intravenously to the rabbits (1) kills bacteria and eliminates bacteremia 30 min post challenge; (2) inhibits Toll-like receptor 4 agonists in serum 90 min post challenge; (3) reduces serum levels of pro-inflammatory cytokines (interleukin-6 and tumor necrosis factor α); and (4) reverts to hypothermia and gives rise to temperature values indistinguishable from basal levels 330 min post challenge. The two components of the combination displayed synergism in some of these activities, and Pep19-2.5 notably counteracted the endotoxin-inducing potential of ceftriaxone. Thus, the combination therapy of Pep19-2.5 and ceftriaxone holds promise as a candidate for human sepsis therapy.
Martina Aida Angeles,Bastien Cabarrou,Antonio Gil-Moreno,Asunción Pérez-Benavente,Emanuela Spagnolo,Agnieszka Rychlik,Carlos Martínez-Gómez,Frédéric Guyon,Ignacio Zapardiel,Denis Querleu,Claire Illac 대한부인종양학회 2021 Journal of Gynecologic Oncology Vol.32 No.6
Objective: We sought to evaluate the impact on survival of tumor burden and surgical complexity in relation to the number of cycles of neoadjuvant chemotherapy (NACT) in patients with advanced ovarian cancer (OC) with minimal (CC-1) or no residual disease (CC-0). Methods: This retrospective study included patients with International Federation of Gynaecology and Obstetrics IIIC–IV stage OC who underwent debulking surgery at 4 high- volume institutions between January 2008 and December 2015. We assessed the overall survival (OS) of primary debulking surgery (PDS group), early interval debulking surgery after 3–4 cycles of NACT (early IDS group) and delayed debulking surgery after 6 cycles (DDS group) with CC-0 or CC-1 according to peritoneal cancer index (PCI) and Aletti score. Results: Five hundred forty-nine women were included: 175 (31.9%) had PDS, 224 (40.8%) early IDS and 150 (27.3%) DDS. Regardless of Aletti score, median OS after PDS was significantly higher than after early IDS or DDS, but the survival difference was higher in women with an Aletti score <8. Among patients with PCI ≤10, median OS after PDS was significantly higher than after early IDS or DDS. In women with PCI >10, there were no differences between PDS and early IDS, but DDS was associated with decreased OS. Conclusion: The benefit of complete PDS compared with NACT was maximal in patients with a low complexity score. In patients with low tumor burden, there was a survival benefit of PDS over early IDS or DDS. In women with high tumor load, DDS impaired the oncological outcome.
Ovarian Stromal Hyperplasia: A Rare Cause of Postmenopausal Hyperandrogenism
Teresa Lozoya Araque,Isauro Rogelio Monfort Ortiz,José Enrique Martín González,Alenda Jiménez García,Inmaculada Navarro Hidalgo,Verónica Andrade Gamarra,Cecilia Parrell Soler,Fernando Gil Raga 대한폐경학회 2020 대한폐경학회지 Vol.26 No.1
Ovarian hyperthecosis and ovarian stromal hyperplasia (OSH) are two uncommon non-neoplastic causes of ovarian hyperandrogenism, whose etiology is still unknown. These conditions are characterized by obesity, hyperinsulinemia, acanthosis nigricans, and even virilization, mainly in postmenopausal women. Here we have reported the case of a 67-year-old patient with a diagnosis of OSH, which was resolved after bilateral laparoscopic oophorectomy. In this case report, we have discussed two different conditions posing a diagnostic challenge and requiring a high index of suspicion.
Consuelo G. Corrales-Maldonado,Vargas-Arispuro Irasema,Luis Martínez-Carrillo José,Pérez-Morales Rosalva,Ángel Martínez-Téllez Miguel,Aispuro-Hernández Emmanuel,Arellano-Gil Maritza,Castro-Espinoza Lu 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.4
Acrobasis nuxvorella Neuzing (Lepidoptera: Pyralidae), or the Pecan Nut Casebearer (PNC), is a monophagous pecan nut [Carya illinoinensis (Wang) K. Koch] herbivore. This insect has caused major crop losses, despite pecan nut trees producing a high concentration of tannins, which are deterrent compounds for insects. The PNC consumes and processes tannin-rich tissues without any negative effects on its nutrition. Certain bacterial species of the herbivore gut microbiota have been proven to produce tannase, therefore, we hypothesize that the PNC has symbiotic bacteria that produce tannase for the digestion of tannin contained in their food. In this work, live PNC larvae were extracted from pecan nutlets. The larval guts were dissected and their contents were cultured to obtain the cultivable bacteria. A total of 224 bacterial isolates were recovered, 19 of which tested positive for tannase activity. Isolates LB66, LB24, TT29, and TP8 displayed comparable activity to the control strain Pseudomonas aeruginosa. Further enzyme semi-purification steps showed specific tannase ac tivity values in the range of 39.5–3.7 U/mg of protein. The isolates were identified as Bacillus pumilus strain TT29, Bacillus pumilus strain LB66, Bacillus pumilus strain LB24 and Sthaphylococcus warneri (TP8) by 16S ribo somal RNA gene sequencing. Our findings indicate that PNC larvae contain gut bacteria able to produce tannase that hydrolyze the galloyl ester bonds in tannins.
C.J. González-Pérez1,I. Vargas-Arispuro,E. Aispuro-Hernández,C.L. Aguilar-Gil,Y.E. Aguirre-Guzmán,A. Castillo,A. Hernández-Mendoza,J.F. Ayala-Zavala,M.A. Martínez-Téllez1 한국미생물·생명공학회 2019 한국미생물·생명공학회지 Vol.47 No.2
The consumption of fresh vegetables has been related to recurrent outbreaks of foodborne diseases (FBD) worldwide. Therefore, the development of effective alternative technologies is necessary to improve the safety of these products. This study aimed to isolate and identify epiphytic lactic acid bacteria (LAB) from fresh fruits and leafy vegetables and characterize their antagonistic capacity due to their ability to produce bacteriocins or antibacterial compounds. For this, 92 LAB isolates from fruits and leafy vegetables were screened for antagonistic activity. Two strains with the highest and broadest antagonistic activities were selected for further characterization; one from cantaloupe melon (strain CM175) and one from cilantro leaves (strain C15). The cell-free supernatants (CFS) of CM175 and C15 were found to exhibit antagonistic activity against FBD-causing pathogens. The CM175 and C15 strains were identified as Pediococcus pentosaceus and Lactobacillus graminis, respectively. Notably, the P. pentosaceus CM175 CFS stopped the growth of Salmonella Typhimurium, Salmonella Saintpaul, Staphylococcus aureus, and Listeria monocytogenes, and delayed Escherichia coli O157:H7 growth. Moreover, L. graminis C15 CFS delayed the growth of all indicator pathogens, but did not completely stop it. Organic acids and bacteriocin-like molecules were determined to be possibly exerting the observed antagonistic activity of the identified LAB strains. Thus, application of the antagonistic compounds produced by Pediococcus pentosaceus and Lactobacillus graminis could be a novel and ecological strategy in developing antimicrobial biopreservatives for the food industry and mitigating FBD by reducing the biological contamination in fruit and vegetable orchards, mainly via their potential in controlling both gram-negative and gram-positive pathogenic bacteria.