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Song, Geun Seoup,Uhm, Tai Boong 한국산업미생물학회 1990 한국미생물·생명공학회지 Vol.18 No.5
Bacillus subtilis의 세포내 아눌라아제가 부분정제되고 그의 작용 모드와 일반적 특성이 조사되었다. 이 효소는 gel filtration에 의하여 분자량을 추정하였을 때 49,000이었고, 등전점은 5.2이었다. 기질에 대한 친화성의 지표인 K_m값은 설탕에 대해서는 10mM, 라피노오스에 대해서는 18mM이었다. 이 효소는 산성쪽에서는 질로 사용, inulinase 활성측정 경우와 동일한 방법에 따라 측정하였다. 단, invertase 활성은 1분간에 2umole의 환원당을 생산하는 효소량을 1단위로 표시하였다. An intracellular inulase(β-fructofuranoside fructohydrolase, EC 3.2.1.26) from Bacillus subtilis has been partially purified and its mode of action and general properties were studied. The enzyme had an apparent molecular weight of 49,000 as estimated by gel filtration and its pI point was 5.2. Substrate concentration studies showed an apparent K_m of 10 mM for sucrose and of 18 mM for raffinose. The enzyme was an acid-labile protein with a pH optimum of 6.6. The optimum temperature was 50℃. The enzyme acts on straight chain oligo-and poly-fructosides of the inulin series via a exo-wise cleavage mechanism, as well as on sucrose.
미나리(Oenanthe stolonifera DC.)의 향기성분
송근섭(Geun-Seoup Song),권용주(Yong-Ju Kwon) 한국식품영양과학회 1990 한국식품영양과학회지 Vol.19 No.4
미나리(Oenanthe stolonifera DC)의 향기성분을 상압 수증기 증류법으로 추출하여 GC 및 GCMS로 분석한 결과 63가지 성분이 분리되었고, 그중에서 탄화수소류 20종(61.94%), alcohol류 9종(8.76%), ester류 1종(1.34%), aldehyde류 1종(2.29%), ketone류 3종(11.52%) 및 miscellaneous 5종 (2.35%) 등 39가지 성분을 동정하였다. 미나리 향기성분은 주로 탄화수소류(61.94%)로 구성되어 있었으며, 주성분은 limonene(12.12%), pulegone(9.48%), germacrene D(8.34%) 및 β-pinene(7.68%)등이었다. The volatile fraction of Oenanthe stolonifera DC. was extracted by a steam distillation under the atmospheric pressure and analyzed by gas chromatography(GC) and gas chromatography/mass spectrometry(GC-MS). The experimental results revealed the presence of 63 volatile components. Among them 39 components identified were composed of 20 hydrocarbons (61.94%), 9 alcohols(8.76%), 3 ketones(11.52%), 1 ester(1.34%), 1 aldehyde(2.29%) and 5 miscellaneous(2.35%). The major volatile components of Oenanthe stolonifera DC. were limonene(12.12%), pulegone(9.48%), germacrene D(8.34%) and α-pinene(7.68%).
Studies on Fungal Lipids Containing γ - Linolenic Acid - 1. Fatty Acid Composition of Mucor sp.
송근섭(Geun-Seoup Song),김충기(Choong-Ki Kim),권용주(Yong-Ju Kwon),양희천(Hee-Cheon Yang),이태규(Tae-Kyoo Lee) 한국식품영양과학회 1990 한국식품영양과학회지 Vol.19 No.6
감마-리놀렌산 생산능이 있는 새로운 곰팡이 균주를 탐색하기 위하여 토양으로부터 감마-리놀렌산 생산능이 있는 3개의 곰팡이 균주를 분리하였으며, 이들 균주를 진탕 배양을 행하여 균체성장, 지질생성량 및 지방산조성을 비교 조사하였다. 3가지 균주중에서 FA-007 균주성장도 양호하였고, 지질함량이 건조균체중 21.1%, 감마-리놀렌산 함량이 총지방산중 15.6%로 높은 특성을 나타내었다. 이 균주는 형태학적 특성을 조사한 결과 Mucor sp.로 동정되었다. 이 균주가 생산하는 지질은 중성지질이 75.2%, 당지질이 5.3% 및 인지질이 19.5%로 구성되어 있었고, 중성지질과 인지질에서의 감마-리놀렌산 함량은 각각 15.5% 및 18.3%를 차지하였다. For a study on the production of y-linolenic acid(GLA) by fungi, 3 fungal strains were isolated from soil. Their cell growth, lipid content and fatty acid composition were compared in shake flask culture. Among these fungi, the fungus, designated as FA-007, has high lipid content(21.1%) and GLA content(15.6% of total fatty acids). The fungal strain FA-007 was tentatively identified as Mucor sp. on the basis of morphological characteristics. Fungal oil produced by this fungus was composed of 75.2% neutral lipid, 5.3% glycolipid and 19.5% phospholipid. Although the GLA content in phospholipid was higher than it in neutral lipid, the GLA content in neutral lipid was high as 15.5%.
Effects of Cheonggukjang and Doenjang on Bone Loss in Ovariectomized Rats
Chang-Hyun Lee,Geun-Seoup Song,Young-Soo Kim 한국식품과학회 2008 Food Science and Biotechnology Vol.17 No.3
The effects of cheonggukjang and doenjang on bone mineral density, trabecular area and cortical thickness of the tibia, and serum osteocalcin level in ovariectomized rats were investigated. After 4 weeks, bone mineral density, bone trabecular area, the cortical thickness index, and serum osteocalcin level were analyzed. The cheonggukjang and doenjang diet groups showed significant prevention of ovariectomized (OVX)-related body weight gain. Whole body bone mineral density of the OVX group was significantly lower than that of the sham group, whereas the cheonggukjang and doenjang diets resulted in complete restoration of bone mineral density. Trabecular area in the proximal diaphysis and cortical thickness in the distal diaphysis of the tibia were increased significantly in the cheonggukjang and doenjang diet fed groups. The cheonggukjang and doenjang diets significantly reduced serum osteocalcin level in the OVX rats. These results suggest that cheonggukjang and doenjang might have inhibitory effects on osteoporosis, by showing accelerated bone formation in OVX rats.