Reference Map of Soluble Protenis from Salmonella enterica Serovar Enteritidis by Two-Dimensional Electrophoresis

김곤섭,Eung-goo Lee,Yong-hwan Kim,정태성,Yong-seung Shin,Gee-wook Shin,Hui-guen Cha,Mi-rim Park 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

Map of Soluble Proteins from Salmonella enterica Serovar Enteritidis byTwo-Dimensional ElectrophoresisMi-rim Park, Eung-goo Lee, Yong-hwan Kim, Tae-sung Jung, Yong-seung Shin, Gee-wook Shin, Hui-guen Cha and Gon-sup Kim*Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, KoreaReceived May 17, 2003 / Accepted July 23, 2003J. Vet. Sci. (2003), 4(2), 143-149JOURNAL OFVeterinaryScience*Corresponding author: Gon-sup Kim Laboratory of Veterinary Biochemistry, Collage of Veterinary Medicine, Gyeongsang National University, 900 Gazwa, Jinju 660-701 Korea Tel: +82-55-751-5823, Fax: +82-55-751-5803 E-mail: gonskim@nongae.gsnu.ac.kr

담관 낭선암 1례

박건욱,홍기봉,정재열,노명환,한상영,최석렬,신우원,나서희 동아대학교의과대학 1997 동아의대학술지 Vol.9 No.1

Mo-silicide의 확산 메카니즘에 관한 연구

박종윤,이상균,한기평,이재욱 성균관대학교 1991 論文集 Vol.42 No.1

n-type Si water(100) 위에 Mo를 1000Å을 증착한 후 400℃에서 120분간 pre-annealing(예비 열처리)하고 기존의 FA(Furnace Annealing) 방법과 본 실험실에서 개발, 제작한 RTA(Rapid Thermal Annealing) 장치로 post-annealing하여 hexagonal MoSi_2와 tetragonal MoSi_2를 형성하였다. 이는 XRD로 phase를 확인하였으며 AES depth profile를 이용하여 Si의 확산 메카니즘을 조사하였다. 기판의 온도가 400℃인 경우 MoSi_2의 형성이 용이하고 표면의 grain의 morphology 또한 FA방법에 비해 RTA방법이 크고 균일하게 형성되고 pre-annealing한 시편이 균일한 grain을 형성함을 알았다. 이는 grain의 morphology가 확산에 커다란 영향을 미치고 있음을 암시하고 있다. Mo films with a thickness of 1000Å were prepared on n-type Si(100) substrates by electron beam evaporation and heated to form the MoSi_2 of the hexagonal-and tetragonal-phase by the FA(Furnace Annealing) and RTA(Rapid Thermal Annealing) methods. The phases of Mo-silicide were confirmed by XRD. AES depth profiles were performed to obtain the Si distribution by the diffusion. Also, the change of MoSi_2 surface morphology was analyzed by SEM and EDX to investigate the mechanism of diffusion. When the substrate temperature was 400℃, well defined MoSi_2 was formed easily, the morphology of grain was distributed largely and uniformly. And it was found that the performance of the pre-annealing before post-annealing lead to lower about 150℃ for the formation temperature of very stable MoSi_2.

연속파형 Nd:YAG 레이저에 의한 인코넬 600의 용접 공정변수의 연구

배신철,유영태,송성욱,신호준,나기대 朝鮮大學校 機械技術硏究所 2004 機械技術硏究 Vol.7 No.1

The laser welding process is one of the most advanced manufacturing technologies owing to its high speed and deep penetration. Welding characteristics of Inconel 600 Alloy using a continuous wave Nd:YAG laser are experimentally investigated. The major process parameters studied in the present laser welding experiment were position of focus, laser power, travel speed. The gap and offset maintained as small as possible. Optical microscope were used to investigate the microstructures of the welded zone. The follow conclusions can be drawn the laser power and travel speed have a pronounced effect the fusion zone size and shape.

Prevalence of plasmid-mediated quinolone and tetracycline resistance genes in Aeromonas strains isolated from eel (Anguilla japonica) and ornamental fish

( Gee-wook Shin ),( Jun-hwan Park ),( Hui-ju Kim ) 한국어병학회 2023 한국어병학회지 Vol.36 No.2

This study investigated the genetic determinants of plasmid-mediated antibiotic resistance (PMAR) to quinolones and tetracycline in 106 Aeromonas strains isolated from eel (Anguilla japonica, 70 strains) and ornamental fish (36 strains) in Korea. Quinolones and tetracycline resistance phenotypes were found to be widely distributed throughout the both fish groups. However, the prevalence of qnr and tet genes was higher in ornamental fish strains than in eel strains (42.9% vs. 86.1% for qnr and 51.4% vs. 69.4% for tet). In addition, the profiling of the present genetic determinants revealed the dominance of qnrS, tetA, tetE and tetE+qnrS genes for eel strains but of tetA+qnrS qnrS and tetE+qnrS genes for ornamental fish strains. These results indicate that aquaculture and related industries could be a major threat to public health due to the possible spread of PMAR.

터빈설비의 정비이력을 이용한 고장확률 예측 및 정비주기 설정에의 응용

송기욱(Gee Wook Song),구재량(Jae Raeyang Koo),최우성(Woo Sung Choi) 대한기계학회 2010 大韓機械學會論文集A Vol.34 No.9

터빈설비 각 중요부품의 고장은 발전정지라는 큰 파급효과를 유발하며, 예기치 못한 고장으로 설비의 이용률이 감소하게 되면 막대한 경제적 손실이 발생한다. 현재 발전설비는 제작사에서 제시한 정비주기를 기준으로 보수적인 예방정비를 실시하고 있으나, 급변하는 경영환경에서 경쟁력을 유지하기 위해서는 신뢰도를 유지하면서 정비비용을 절감하는 신뢰도 기반 정비방법을 도입 해야 할 필요가 있다. 신뢰성 있는 정비주기를 선정하기 위해서는 설비의 고장이력에 대한 면밀한 분석을 통하여 고장확률을 예측해야 한다. 본 논문은 발전설비 중 터빈 각 부품들의 고장이력을 데이터베이스로 만들고, Weibull 함수를 이용하여 최적의 정비시점을 예측하며, 정비주기를 결정하는 방법에 대하여 연구하였다. The breakdown of any critical component of a turbine results in the outage of power plants. Unexpected failure decreases equipment utilization and causes enormous economic losses. Currently, we conduct conservative preventive maintenance for a maintenance period that is proposed by a vendor. In the rapidly changing business environment, reliability-based maintenance is required in order to remain competitive and reduce maintenance costs while maintaining the reliability of equipment. In order to determine an appropriate maintenance period for guaranteeing reliability, we must determine the failure probability by carefully analyzing the failure history of the equipment. In this study, we created a database of failure history for power-plant turbines, predicted the best repair time using the Weibull function, and investigated how the appropriate maintenance cycle can be determined.

Life Prediction of High Pressure Steam Turbine Rotor

Song, Gee Wook,Kim, Bum Shin,Hyun, Jung Seob Trans Tech Publications, Ltd. 2006 Key Engineering Materials Vol.321 No.-

<P>The operation mode of thermal power plants has been changed from the base load to duty cycle. From the changeover, fossil power plants cannot avoid frequent thermal transient state, for example, start up and stop, which results in thermal fatigue damage at the heavy section components. The rotor is the highest capital cost component in steam turbine and requires long outage for replacing the new one. For optimized power plant operation life and inspection management of rotor is necessary. It is known as general that start-up and shutdown operation greatly affect on steam turbine life. The start-up operation condition is especially severe because of the rapid temperature and rotational speed increase, which causes damage and reduction of main components life of steam turbine. The start-up stress of rotor which is directly related life is composed of thermal and rotational stresses. The thermal stress is due to the variation of steam flow temperature and rotational stress is due to the rotation speed of itself. In this paper, the analysis method of start-up stress of rotor which considers simultaneously temperature and rotation speed transition is proposed, which includes a case study regarding 500MW fossil power plant steam turbine rotor. Also, the method of damage quantitative estimation of fatigue damage to operation condition is described. The method can be applied to find weak points to the fatigue damage. Using the method, total life consumption can be obtained, and can be also used for determining future operation mode and the life extension of old fossil power units.</P>

Comparison of antigenic proteins from <i>Lactococcus garvieae</i> KG (−) and KG (+) strains that are recognized by olive flounder (<i>Paralichthys olivaceus</i>) antibodies

Shin, Gee-Wook,Nho, Seong-Won,Park, Seong-Bin,Jang, Ho-Bin,Cha, In-Seok,Ha, Mi-Ae,Kim, Young-Rim,Dalvi, Rishikesh S.,Joh, Seong-Joon,Jung, Tae-Sung Elsevier 2009 Veterinary microbiology Vol.139 No.1

<P><B>Abstract</B></P><P><I>Lactococcus garvieae</I> is an important etiological agent of lactococcosis in various fish species including olive flounder (<I>Paralichthys olivaceus</I>). In this study, proteomic and immunoproteomic analyses were employed to compare the antigenic profiles of strains KG9408, MS93003, and NSS9310 strains of <I>L. garvieae</I>. Proteomic analysis using two-dimensional gel electrophoresis (2-DE) revealed differences in five protein spots among the different <I>L. garvieae</I> strains. In immunoproteomic analysis, there was a significant difference in the 2-DE immunoblot profiles of the <I>L. garvieae</I> strains using sera collected from fish surviving infection with either <I>L. garvieae</I> strains KG9408 or NSS9310. These sera reacted with 8 and 7 unique antigenic protein spots, respectively. Heat shock protein (HSP) 70 and DNA-directed RNA polymerase were among the specific antigens recognized by the anti-NSS9310 serum. In addition, the anti-NSS9310 and anti-KG9408 olive flounder sera reacted with 25 common antigenic protein spots of all the <I>L. garvieae</I> strains, which included elongation factor (EF)-Tu, arginine deiminase (AD), inosine-5′-monophosphate dehydrogenase (IMPD), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), phosphomannomutase (PMM), <SMALL>L</SMALL>-lactate dehydrogenase (<SMALL>L</SMALL>-LDH), 6-phosphofructokinase and UDP-galactose 4-epimerase (UDP-galactose). Based on the present results, the 8 antigens recognized by the anti-KG9408 serum and the 25 common antigens recognized by both sera may serve as potential markers for developing an effective vaccine against this bacterium.</P>

저압터빈 최종단 블레이드 손상해석

송기욱(Gee Wook Song),최우성(Woo Sung Choi),김완재(Wanjae Kim),정남근(Nam Gun Jung) 대한기계학회 2013 大韓機械學會論文集B Vol.37 No.12

증기터빈의 터빈 블레이드는 발전소 핵심설비 중 하나로, 로터의 디스크에 결합되어 회전함으로써 증기 에너지를 기계적 에너지로 변환시켜주는 역할을 하고 있다. 최근 터빈의 잦은 기동정지로 인해 블레이드 회전에 따른 원심하중이 반복적 작용하고 이에 따른 저압 증기터빈 최종단 블레이드의 손상이 자주 보고되고 있다. 본 논문에서는 터빈 블레이드에 발생되는 손상을 분석하여 블레이드에 발생되는 저주기 피로수명을 평가하였다. 증기터빈 최종단 블레이드의 균열발생 수명을 결정하기 위해 유한요소법으로 계산한 탄성응력에 Neuber’s rule을 적용하여 진변형율 진폭을 계산하였으며, 예측된 수명과 블레이드 실제 기동정지횟수가 잘 일치됨을 보였다. A steam turbine blade is one of the core parts in a power plant. It transforms steam energy into mechanical energy. It is installed on the rim of a rotor disk. Many failure cases have been reported at the final stage blades of a low-pressure (LP) turbine that is cyclically loaded by centrifugal force because of the repeated startups of the turbine. Therefore, to ensure the safety of an LP steam turbine blade, it is necessary to investigate the fatigue strength and life. In this study, the low cycle fatigue life of an LP steam turbine blade is evaluated based on actual damage analysis. To determine the crack initiation life of the final stage of a steam turbine, Neuber’s rule is applied to elastic stresses by the finite element method to calculate the true strain amplitude. It is observed that the expected life and actual number of starts/stops of the blade were well matched.

터빈설비의 정비이력을 이용한 고장확률 예측

송기욱(Gee Wook Song),구재량(Jae Raeyang Koo),최우성(Woo Sung Choi) 대한기계학회 2010 대한기계학회 춘추학술대회 Vol.2010 No.5

터빈설비 각 중요부품의 고장은 발전정지라는 큰 파급효과를 유발하며, 예기치 못한 고장으로 설비의 이용률이 감소하게 되면 막대한 경제적 손실이 발생한다. 현재 발전설비는 제작사에서 제시한 정비주기를 기준으로 보수적인 예방정비를 실시하고 있으나, 급변하는 경영환경에서 경쟁력을 유지하기 위해서는 신뢰도틀 유지하면서 정비비용을 절감하는 신뢰도 기반 정비방법을 도입해야 할 필요가 있다. 신뢰성 있는 정비주기를 선정하기 위해서는 설비의 고장이력에 대한 면밀한 분석을 통하여 고장확률을 예측해야 한다. 본 논문은 발전설비 중 터빈 각 부품들의 고장이력을 데이터베이스로 만들고, Weibull 함수를 이용하여 최적의 정비시점을 예측하며, 정비주기를 결정하는 방법에 대하여 연구하였다. A breakdown of each critical component of turbine results in outage of power plant. Unexpected failure decreases equipment utilization and occur the enormous economic losses. Currently, we conduct a conservative preventive maintenance based on maintenance period proposed by vendor. In a rapidly changing business environment, we should be introduced to the reliability-based maintenance in order to remain competitive and reduce maintenance costs while maintaining the reliability of the equipment. We should predict the probability of failure through a careful analysis of failure history on the equipment in order to select reliable maintenance period. In this paper, we created a database of failure history for power plant turbines, predicted the best repair time using Weibull function, and how to determine the maintenance cycle has been studied.