3'-Methyl-4-dimethylaminoazobenzene이 백서의 정상 및 재생간의 EGF Receptor 변동에 미치는 영향

홍장희,허강민,석정호,이재흔 충남대학교 의과대학 지역사회의학연구소 1997 충남의대잡지 Vol.24 No.1

To study the changes of EGF receptors in the rat liver regeneration and hepatic carcinogenesis, ^125I-EGF binding sites were measured in the regenerated liver cell membrane after partial hepatectomy of normal rat and 3-methyl-4-dimethylaminoazobenzene treated rat. 1. EGF binding sites (Bmax) in the normal rat liver cell membrane were 553.8fmole/mg protein and were markedly decreased at the 1st day after partial hepatectomy. These sites were increased at the 3rd day(Bmax-161.0fmole/mg protein) and were 29% of the normal liver EGF binding sites. 2. EGF binding sites (Bmax) in the normal cell membrane of the 3-methyl-4-dimethylaminoazobenzene-treated rat, were 72.8fmole/mg protein and were significantly less than those in the normal rat live cell membrane. At the 1st day after partial hepatectomy, EGF binding sites were markedly decreased and increased at the 3rd day (Bmax=155.8fmole/mg protein). 3. Kd values in EGF receptor binding were not much altered in both liver cell membranes of the normal rat and the 3-methyl-4-dimethylaminoazobenzene treated rat. From these result, it is suggested that decreased EGF binding sites in the liver cell membrane of regenerating rat liver and 3-methyl-4-dimethylaminoazobenzene treated rat may be due to the alteration of EGF receptor number.

골절의 유형과 정도에 따른 전두동 골절의 치료 : 23예의 분석결과

진홍률,신시옥,최영석,송창근,용만강 대한이비인후과학회 2000 대한이비인후과학회지 두경부외과학 Vol.43 No.4

Nicotine이 발생중인 鷄胎의 體重 成長에 미치는 영향

金武剛,金弘善,柳時潤,申台均,李永浩,趙聲煥 충남대학교 의과대학 지역사회의학연구소 1985 충남의대잡지 Vol.12 No.2

The authors studies the effects of Nicotine on the Body weight growth of the developmental chick embryo. On the sixth day of incubation, 0.41mg, 0.82mg and 1.65mg of Nicotine were injected into the chorioallantoic sac of each egg of three groups and one group was injected physiological saline solution as a control, and the eggs were broken for weighting the body weight in every other day from the twelfth day to eighteenth day of incubation. In an attempt to analyze the growth of the body weights, growth formula of Zimmerman and relative growth formula of Huxley were applied. The following results were obtained. 1. The growth of chick embryo boby weight was affected as inhibiting by the Nicotine. The inhibiting effects were in proportion to the injected Nicotine dosage. 2. The growth quantity of the body weight injected 1.65mg was almost half weight of the control group body weight. 3. Growth rate was decreased continuously with tine until 18th day in the 1.65mg injected group but it was increased in 0.41mg and 0.82mg injected group with time lapse from 12th day to 18th day, and 0.41mg injected group was more increased than 0.82mg injected group. 4. Specific growth rate decreased in the all of 0.41mg, 0.82mg and 1.65mg injected group and in the decrease degree 1.65mg and 0.82mg injected group ware severe and 0.41mg injected group was slight. 5. Growth centers of growth gradient were 12th day in the 0.41mg injected group and 18th day in the 0.82mg and 1.65mg injected groups. 6. Relative growth rate was also decreased proportionally in related with injected Nicotine dosage.

滿族薩滿敎的內容與特色

王宏剛 漢陽大學校 民族學硏究所 1999 民族과文化 Vol.8 No.-

지체부자유학생을 위한 학교교육과정 적용에 따른 교사ㆍ학부모의 인식 수준

한홍석,강성종 국립특수교육원 2005 특수교육연구 Vol.12 No.2

현행 국가수준의 교육과정기준은 지체부자유학생을 위한 학교교육과정을 편성ㆍ적용하도록 하고 교육과정 내용을 다양화ㆍ적합화 하도록 하는 계기를 마련하고 있으나 학교 현장에서는 정착화 되지 못하고 있는 실정이다. 이에 본 연구는 지체부자유학생을 위한 학교교육과정 적용에 따른 교사와 학부모가 인식하는 수준을 조사하여 그 결과를 토대로 학교교육과정 적용 방안을 제시하는데 목적을 두었다. 연구의 결과, 현행 지체부자유학생을 위한 학교교육과정의 인식 수준이 매우 낮음과 동시에 변화를 촉구하고 있음을 확인하는 계기가 되었다. 따라서 지체부자유학생을 위한 학교교육과정 적용을 정당화하기 위한 적용 방안을 제시하였다. It was April. 15, 1967 when the special school's curriculum was made for the first time in Korea. Since then, the special curriculums for the physically disabled learners have been made and revised in different times for each type of disabled learner, but based on the time when the curriculum for general schools was revised for the first time, at present, the 7th curriculum for special schools, which was revised from the 6th curriculum, is being implemented. Also, evidently, much development has been achieved in the curriculum of special schools. The current standard of the curriculum under the Korean government makes the school curriculum for the physically disabled learners additionally organized and performed. Also, it provides an opportunity to make the contents of curriculum varied and fit for the physically disabled learners. However, this measure fails to be properly applied to the real school. Accordingly, this study has a purpose of researching how much the teacher, a subject of education for the physically disabled learners, and parents, who are educational recipients, are satisfied with the application of the special curriculum for the physically disabled learners. Then, based on the result of the research, we aimed at coming up with the measures for applying this special curriculum to the real educational status of schools. According to this study, it was disclosed that the current school curriculum for the physically disabled learners was regarded as unfit. Also, through this study, we could confirm that teachers and parents are pursuing new changes in curriculum. Therefore, We came up with the measures for justifying that the special curriculum is applied to schools.

韓國人 手掌皮膚 Meissner 小體의 量的 變化에 關한 硏究

金弘善,金武剛,趙聲煥,柳時潤 충남대학교 의과대학 지역사회의학연구소 1983 충남의대잡지 Vol.10 No.2

In order to observe the quantitative changes in Meissner's tactile corpuscles caused by aging and in different areas, 3×5 ㎟ punch excision specimens in the palm of the right and left hand from the 46 cadavers (male 35, female 11) were collected, sectioned serially, stained by the methylene blue and observed with light microscope and then analyzed a numerical changes in the palm of the right and left hand and in the site 6 and 7 in the palm of the hand. The results are summerized as follows: 1. The mean concentration of Meissner's corpuscles in the palm of the right hand was 2.92 per square millimeter and in the palm of the left hand was 5.17. There was appeared the mean concentration of Meissner's corpuscles in the palm of the hand was greater in the left than in the right. 2. In the site 6, the mean concentration of Meissner's corpuscles was 3.93 per square millimeter in the age group 21 to 50 years, 2.96 in the 51 to 60 years, 2.52 in the 61 to 83 years. There was a decrease in the mean concentration of Meissner's corpuscles with increasing age. 3. In the site 7, the mean concentration of Meissner's corpuscles was 5.51 per square millimeter in the age group 21 to 50 years, 4.98 in the 51 to 60 years, 4.94 in the 61 to 78 years. Also, there was a decrease in the mean concentration of Meissner's corpuscles with increasing age. 4. In the site 6, the mean concentration of Meissner's corpuscles in the palm of the right hand was 3.57 per square millimeter in the age group 21 to 50 years, 2.74 in the 61 to 78 years and in the palm of the left hand was 3.07 in the 21 to 60 years, 2.24 in the 61 to 83 years. There was appeared the mean concentration of Meissner's corpuscles in the site 6 of the palm was greater in the right than in the left. 5. In tie site 7, the mean concentration of Meissner's corpuscles in the palm of the right hand was 5.12 per square millimeter in the age group 21 to 78 years and in the palm of the left hand was 5.13 in the 21 to 78 years. There was appeared the mean concentration of Meissner's corpuscles in the site 7 of the palm was a little greater in the left than in the right.

골격근 근형질세망의 ATPase활성도에 대한 Vanillylnonanamide의 영향

박경섭,홍장희,류영수,성지연,허강민,임종호,이재흔,석정호 충남대학교 의과대학 지역사회의학연구소 1999 충남의대잡지 Vol.26 No.2

To investigate the effect of vanillylnonanamide(VN) on the ATPase activity of the sarcoplasmic reticulum(SR) of the skeletal muscle, we prepared the SR vesicles from the back muscle of the rabbit, and measured ATPase activity. The results as follows: Ca-ATPase activity was about 50% in the total ATPase activity of skeletal muscular SR. In the reaction mixture with calcium, 100μM VN increased ATPase activity to 20%, and 100 nM and 10 μM thapsigargin(THP) inhibited the ATPase activity to 50% and 60%, respectively. And 100 μM VN plus 100 nM or 10 μM THP more inhibited ATPase activity than THP alone did it. However, without calcium, 100μM VN did not affect ATPase activity, and 10 μM THP inhibited it to 41%, but VN plus THP inhibited it to 31%. The above results show that VN in the reaction mixture with or without calcium has the different action to ATPase activity when it is used alone or together with Ca-ATPase inhibitor THP. This suggests that VN might increase or decrease the skeletal SR Ca-ATPase activity through affecting the lipid membrane around the Ca-ATPase.

생쥐 鼻中隔 各種 粘膜上皮 및 粘膜下腺의 分布樣狀과 그 表面積에 관한 硏究

金東煥,金武剛,申台均,金弘善,柳時潤,李永浩 충남대학교 의과대학 지역사회의학연구소 1986 충남의대잡지 Vol.13 No.1

In order to set the distribution pattern and surface area of the mucosal epithelium and submucosal gland in the mice nasal septum. 3-month-old healthy 6 mice weighing 25gm approximately have been contributed for this research. For the mapping of the each nasal epithelium and submucosal gland in the submicroscopic level, the routine paraffin block of the mice nasal septum were cut as the 10㎛ thick at 110㎛ intervals. Consecutive 2 slides were stained with H-E and PAS and reconstructed in form of maps representing the structure in a sagittal plane. The results obtained from this research were summarized as follows. 1. The unilateral nasal surface epithelial area of the mice nasal cavity was 31.23㎟ 2. The areas of the stratified squamous epithelium, respiratory epithelium and olfactory epithelium which compose the unilateral nasal epithelium were 4.47㎟(14.3%), 12.7㎟(40.8%), and 14.02㎟(44.9%), respectively. 3. Submucosal glands in the mice nasal septum were divided into the PAS-positive, PAS-negative and Bowman's glands by the PAS staining. 4. The surface area of the PAS-positive glands in the nasal unilateral septum was 5.23㎟ which is located in the central area of the respiratory portion. 5. The surface area of the PAS-negative glands in the nasal unilateral septum was 5.05㎟ which is located above and below the PAS-positive gland. 6. The surface area of the Bowman's glands was 12.1 ㎟ which was located in the olfactory region. 7. In the vestibular region., 3 ducts of PAS-negative glands of the respiratory region are found, and below the nasal septum there is the infraseptal gland(PAS-negative).

오리 선위 점액분비세포의 점액질에 대한 조직화학적 연구

류시윤,김홍선,김무강,신태균,이영호 충남대학교 의과대학 지역사회의학연구소 1985 충남의대잡지 Vol.12 No.2

Histochemical sequential staining techniques, PAS, alcian blue pH 2.5 and pH 0.4, were applied to sections from the proventriculus of ducks to observe the histochemical properties of epithelial mucosubstances in the proventriculus of mallard and white Pekin ducks. The whole proventriculus obtained were divided systemically cross strips and processed routinely for histologic and histochemical study. Base on the histologic characteristics, we designated as upper portion which is the distal region of the esophageal-proventriculus junction, as middle portion, and as lower portion which is the proximal region of the proventricular-isthmus junction. The results obtained are as follows 1. Generally in the surface and foveolar epithelia showed the presence of the mixture of neutral and acid mucosubstances. 2. In the tubular gland showed the acidic nonsulfated and sulfated mucosubstance in addition to neutral mucosubstances with occasional neutral and acidic nonsulfated mucosubstances. 3. In the compound gland neutral mucosubstance with scant amounts of acidic mucosubstances or with lack of it were demonstrated. 4. There were recognized differences between speies in the staining intensity of all three types of mucosubstances. 5. There were recognized topographic difference in the acidity of epithelial mucosubstances according to portion.

오리 網膜에 存在하는 神經節細胞의 數와 分布에 關한 硏究

姜翰秀,金武剛,申台均,金弘善,柳時潤,李永浩 충남대학교 의과대학 지역사회의학연구소 1986 충남의대잡지 Vol.13 No.1

The number and distribution of the retinal ganglion cells in the 1-week-old and 7-week-old ducks were determined from whole flat mounted preparations stained with methylene blue and thionin. The results were summarized as follows. 1. In the 1-week-old and 7-week-old ducks, the areas of the retinas were 269.4㎟ and 357.2㎟, respectively, and the total cell numbers 1,742,800 and 1,808,800, respectively. 2. The ganglion cells based upon soma diameter were classified into 3 groups such as small (below 10㎛), medium (10-20㎛) and large (above 20㎛) ganglion cells. In 1-week-old duck, the relative frequencies of these 3 groups were 91.0%, 8.5% and 0.5%, respectively, and in 7-week-old duck, 83.7% 14.7% and 1.6%, respectively. 3. Area centralis was located at the distance of 1.6mm (1-week-old) and 2.0mm(7-week-old) nasal side from the anterior tip of the pecten. The number of ganglion cells in the area centralis was 11,000 cells/㎟ (1-week-old) and 12,000 cells/㎟ (7-week-old). 4. There was a gradual decrease in density of retinal ganglion cells extending from area centralis to the periphery of retina. 5. The density of the large (above 20㎛) ganglion cells was higher in the peripheral regions than in the center of retina. Higher densities were noted in the superior-temporal region in 7-week-old duck, and in the nasal region in 1-week-old duck than in the other regions. 6. The direction of the beak tip is approximately 30° in 1-week-old and 26° in 7-week-old duck from the angle of the pecten.