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Jing Zhai,Weishan Chang,Zhenzhen Zhai,Xingliang Ma,Lingling Yang,Jinfeng Ti,Daozhen Song,Shijun Fu,Dapeng Li 한국유전학회 2016 Genes & Genomics Vol.38 No.8
Few studies have attempted to characterize the pheasant (Phasianus colchicus) immunoglobulin Y (IgY) heavy chain constant region. In the present study, fragments of the pheasant IgY heavy chain constant region were cloned, analyzed, and expressed. The cross-reactivity of IgY or immunoglobulin G (IgG)s with antigens from other vertebrate species was determined using dot-enzymelinked immunosorbent assay and western blot analysis. Five peptides of the pheasant IgY heavy chain constant region were synthesized to determine its immunoregulatory activity in vitro. The IgY heavy chain constant region from pheasant showed the highest homology with that from chicken (71.2 %) and duck (49.1 %). Phylogenetic analysis for IgY showed that pheasant was closely related to chicken and duck than to any other analyzed vertebrate species. The rabbit anti-chicken IgG showed immunologic cross-reactivity with recombinant proteins of the pheasant IgY heavy chain constant region. Four peptides were able to induce significant up-regulation of interleukin (IL)-1b, IL-4, and interferon-c in chicken peripheral blood lymphocytes, suggesting a new role of avian IgY in immune regulation.
The Current Status, Trend, and Influencing Factors to Malnutrition of Infants and Children in China
Zhai, Feng-Ying,Wang, Hui-Jun,Chang, Su-Ying,Fu, Dawei,Ge, Keyou,Popkin, Barry M. The Korean Society of Community Nutrition 2004 Journal of community nutrition Vol.6 No.2
Children are the most nutrition sensitive sub-group of a population. The nutritional status of children should be especially emphasized at all levels. This study was performed to investigate the current status, trend, and influencing factors to malnutrition of infants and children in China. The study was mainly based on the China Health and Nutrition Survey which is a longitudinal study conducted in 8 provinces and the data on growth of children under 7 years of age in 9 cities in China. The result of this study showed that one fifth of the children under 5 years of age are still suffering from stunted growth and one tenth suffering from underweight. The nutrition intervention on children under 2 years of age, especially on those under 18 months should be emphasized. Better supplementary food can improve the nutritional status to decrease the prevalence of stunted children. Therefore, the development of supplementary food should be the priority and should be emphasized with breastfeeding.
The Synthesis and Spectral Properties of Some New Monomethine Cyanine Dyes for Live Cell Imaging
Y. L. Fu,Y. Z. Zhou,G. Zhang,Q. C. Yang,X. Zhai,L. Y. Wang 대한화학회 2015 Bulletin of the Korean Chemical Society Vol.36 No.2
Four monomethine cyanine dyes were synthesized and their structures were confirmed by 1H NMR, IR, UV–Vis, and HRMS. Their spectral properties were studied. It could be found that the λmax of the dyes was shorter in the protonic solvents compared with that in the non-protonic solvents. Moreover, the λmax shows a hypsochromic shift with the increasing polarity of the solvents. The interactions of four dyes with DNA were also studied. The results showed that the fluorescence intensities of the dyes 3a and 3b took place a qualitative change in the presence of DNA compared with the free dyes. The dyes used as cellular imaging agents were researched. The results indicated that the dye 3b could penetrate the live cell membrane.
Expression of IER3 in Primary Hepatocarcinoma: Correlation with Clinicopathological Parameters
Liu, Zhong,Wang, Xin-Mei,Jia, Tong-Fu,Zhai, Yi,Sun, Ling-Yan,Cheng, Yu-Ping,Zhang, Yue-Min,Liu, Shi-Hai,Liang, Jun Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.2
Background: Studies indicate the immediate early response gene 3 (IER3) is involved in many biological processes. Recently, it was discovered that IER3 plays an important role in tumorigenesis and tumor progression. Thus it may be a valuable biomarker in tumor. This study was designed to investigate the expression status of IER3 in primary hepatocarcinoma (PHC) and correlation with clinicopathological parameters. Materials and Methods: Real-time PCR was performed to evaluate the expression levels of IER3 in 62 pathologically diagnosed human PHC specimens. Results: A statistically significant association was disclosed between the expression of IER3 and P53 mutant protein (short for P53), Ki-67, EGFR and the biggest diameter, differentiation grade of tumor. Conclusions: This work is the first to shed light on the potential clinical usefulness of IER3, as an efficient tumor biomarker in PHC.
Study on Thermodynamics of Three Kinds of Benzindocarbocyanine Dyes in Aqueous Methanol Solution
Wei Huang,Lan-Ying Wang,Yi-Le Fu,Ji-Quan Liu,You-Ni Tao,Fang-Li Fan,Gao-Hong Zhai,Zhen-Yi Wen 대한화학회 2009 Bulletin of the Korean Chemical Society Vol.30 No.3
Aggregation behavior of three kinds of benzindocarbocyanine dyes in aqueous methanol solution was studied by UV-Vis absorption spectrum. The results indicated that the three dyes all existed monomer-dimer equilibrium in aqueous methanol solution (concentration range 10−5 to 10−6 M) at 25.0~41.0 °C for Dye 1, 28.0~49.0 oC for Dye 2 and 26.0~47.0 °C for Dye 3. The fundamental property of the three dyes as the dimeric association constant KD, the dimeric free energy ΔGD, the dimeric entropy ΔSD, and the dimeric enthalpy ΔHD were determined. The ΔHD of three dyes: Dye 1, Dye 2 and Dye 3 was -42.5, -15.1 and -18.9 kJ/mol, respectively. The experimental observations were the subject of a theoretical study including the ground-state geometries which were fully optimized using DFT at B3LYP/6-31G level. The effect of dye molecule structure on ΔHD was discussed by theoretical calculations.
Study on Thermodynamics of Three Kinds of Benzindocarbocyanine Dyes in Aqueous Methanol Solution
Huang, Wei,Wang, Lan-Ying,Fu, Yi-Le,Liu, Ji-Quan,Tao, You-Ni,Fan, Fang-Li,Zhai, Gao-Hong,Wen, Zhen-Yi Korean Chemical Society 2009 Bulletin of the Korean Chemical Society Vol.30 No.3
Aggregation behavior of three kinds of benzindocarbocyanine dyes in aqueous methanol solution was studied by UV-Vis absorption spectrum. The results indicated that the three dyes all existed monomer-dimer equilibrium in aqueous methanol solution (concentration range $10^{−5}\;to\;10^{−6}$ M) at 25.0$\sim$41.0 ${^{\circ}C}$ for Dye 1, 28.0$\sim$49.0 ${^{\circ}C}$ for Dye 2 and 26.0$\sim$47.0 ${^{\circ}C}$ for Dye 3. The fundamental property of the three dyes as the dimeric association constant KD, the dimeric free energy ${\Delta$}G_D, the dimeric entropy ${\Delta$}S_D, and the dimeric enthalpy ${\Delta$}H_D were determined. The ${\Delta$}H_D of three dyes: Dye 1, Dye 2 and Dye 3 was -42.5, -15.1 and -18.9 kJ/mol, respectively. The experimental observations were the subject of a theoretical study including the ground-state geometries which were fully optimized using DFT at B3LYP/6-31G level. The effect of dye molecule structure on ${\Delta$}H_D was discussed by theoretical calculations.