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Fu‑xiang Cao,Kun‑kun Deng,Cui‑ju Wang,Kai‑bo Nie,Wei Liang,Jian‑feng Fan 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.12
The 10 μm 10 vol% SiCp/2024Al composites sheet was successfully fabricated by semi-solid stir casting followed withtwo-step deformation (extrusion and rolling). The two-step thermal deformation solves the sheet rolling formability of theSiCp/2024Al composites produced by stir casting. Compared with the SiCp/2024Al composites prepared by traditional powdermetallurgy, this paper innovatively studied the synergistic enhancement between CuAl2phase and SiCpon the mechanicalproperties. The result shows that the two-step deformation can significantly improve the uniformity of the distribution of SiCpand CuAl2phases. At the same time, the average size of the SiCpand CuAl2phase decreases after the two-step deformation. During the hot extrusion, SiCphas an obvious hindrance to the refinement of the CuAl2phase. Moreover, the SiCp/2024Alcomposites sheet exhibits excellent yield strength (295 MPa), ultimate tensile strength (489 MPa), and elongation (10.42%). The performance improvement of SiCp/2024Al after two-step thermal deformation is mainly attributed to the improvedinterface bonding strength of SiCp-2024 Al matrix, the refined SiCpand the more uniform distribution of SiCp.
Molecular characterization of chemosensory protein genes in Bactrocera minax (Diptera: Tephritidae)
Wang Zhao‐Xiang,Qi Zhen‐hua,Chen Jian,Wang Fu‐Lian,Gui Lian‐You,Zhang Guo‐Hui 한국곤충학회 2021 Entomological Research Vol.51 No.7
Chemoreception is of great importance for survival of insects. Chemosensory proteins (CSPs) are believed to be involved in the perireceptor events of chemosensory system in many insect species, but this has not been clarified in the citrus fruit fly, Bactrocera minax, a serious dipteran pest of citrus crops. Previous studies indicated that four CSP genes were identified in many fly speices in Diptera. In the present study, we also identified four CSP genes in B. minax,namely BminCSP1–4, from a transcriptome database. All CSP proteins encoded by these genes bear the typical hallmarks of the CSP family: an N-terminal signal peptide and the four highly conserved cysteine residues. Phylogenetic analysis comparing with other dipteran CSPs indicated that dipteran CSPs may evolved from three ancestral CSP genes, and revealed the sequence diversities of BminCSPs and showed that BminCSP1–4 are clustered in separate groups, indicating the possibility of their contrasting function in B. minax. Furthermore, the tissue distribution of the four CSP genes in adult B. minax was analyzed by real-time quantitiative PCR. The results demonstrated that BminCSP3 are significantly transcriptionally enriched in antennae; BminCSP4 expressed primarily in heads; BminCSP1 and BminCSP2 showed high expressions in different tissues, such as antennae, abdomens and wings. Based on these findings, the different implications for the functions of BminCSPs are discussed. This study will offer a significant indication for further functional studies of the CSPs in B. minax
Orthogonal Optimization Design and Finite Element Analysis of Converging Stepped Magnetic Fluid Seal
Fu-xiang Hao,An-le Mu 한국자기학회 2022 Journal of Magnetics Vol.27 No.2
In order to better solve the problem of air leakage during compressor operation, based on the converging stepped magnetic fluid seal structure, the L16 (4⁴) orthogonal test design and the numerical simulation of the finite element method are combined to optimize the sealing structure. Four factors, four levels and the corresponding orthogonal table are selected in this paper. The simulation results of each test are calculated and range values are studied. Finally, sealing pressure capability of the structure before and after optimization are calculated and compared. The results show that under the conditions of different axial and radial sealing gaps, the sealing pressure capability of converging stepped magnetic fluid seal structure has been significantly improved after orthogonal optimization, especially when the radial sealing gap is relatively small. The maximum pressure capability can be improved by about 11 %, which fully proves the effectiveness of orthogonal optimization. At the same time, the research results can also provide references for the application of other rotary sealing conditions.
Sequential Polyadenylation to Enable Alternative mRNA 3’ End Formation
Xiang-Dong Fu,Yajing Hao,Ting Cai,Chang Liu,Xuan Zhang 한국분자세포생물학회 2023 Molecules and cells Vol.46 No.1
In eukaryotic cells, a key RNA processing step to generate mature mRNA is the coupled reaction for cleavage and polyadenylation (CPA) at the 3′ end of individual transcripts. Many transcripts are alternatively polyadenylated (APA) to produce mRNAs with different 3′ ends that may either alter protein coding sequence (CDS-APA) or create different lengths of 3′UTR (tandem-APA). As the CPA reaction is intimately associated with transcriptional termination, it has been widely assumed that APA is regulated cotranscriptionally. Isoforms terminated at different regions may have distinct RNA stability under different conditions, thus altering the ratio of APA isoforms. Such differential impacts on different isoforms have been considered as post-transcriptional APA, but strictly speaking, this can only be considered “apparent” APA, as the choice is not made during the CPA reaction. Interestingly, a recent study reveals sequential APA as a new mechanism for post-transcriptional APA. This minireview will focus on this new mechanism to provide insights into various documented regulatory paradigms.
Ding, Fu-Xiang,Zhang, Gen-Xi,Wang, Jin-Yu,Li, Yuan,Zhang, Li-Jun,Wei, Yue,Wang, Hui-Hua,Zhang, Li,Hou, Qi-Rui Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.2
The level of genetic differentiation and genetic structure in a Chinese native chicken breed, Bian chicken, and two controlled chicken populations (Jinghai chicken and Youxi chicken in China) were analysed based on 29 microsatellite markers. A total of 166 distinct alleles were observed across the 3 breeds, and 32 of these alleles (19.3%) were unique to only 1 breed. Bian chicken carried the largest number of private alleles at 15 (46.9%), followed by the Jinghai chicken with 12 private alleles (37.5%). The average polymorphism information content (0.5168) and the average expected heterozygote frequency (0.5750) of the Bian chicken were the highest, and those of the Jinghai chicken were 0.4915 and 0.5505, respectively, which were the lowest. Among 29 microsatellite loci, there were 15 highly informative loci in Bian chicken, and the other 14 were reasonably informative loci. The highly informative loci in Jinghai chicken and Youxi chicken were 17 and 14 respectively. Significant deviations from the Hardy-Weinberg equilibrium were observed at several locus-breed combinations, showing a deficit of heterozygotes in many cases. As a whole, genetic differentiation among the breeds estimated by the fixation index (Fst) were at 6.7% (p<0.001). The heterozygote deficit within population (Fis) was 22.2% (p<0.001), with the highest (0.249) in Bian chicken and lowest (0.159) in Youxi chicken. These results serve as an initial step in the plan for genetic characterization and conservation of the Chinese chicken genetic resource of Bian, as well as Jinghai and Youxi chickens.
吳福祥(Fu-Xiang Wu),尹淳一(번역자) 중국어문논역학회 2018 中國語文論譯叢刊 Vol.0 No.42
본고는 문법화 이론의 관점으로 의미지도모형(Semantic Map Model)에서 개념공간(Conceptual Space)을 동태화하는 방법을 논의하였다. 논의의 결과로 개념공간은 기능함축관계에 근거하는 교차언어 비교, 문법화(의미변화1)를 포함) 경로의 활용, 문법화 정도와 원리에 근거하는 공시적 재구 등 세 가지 수단을 근거로 동태화할 수 있음을 밝혔다. 또한 본고는 통시적 개념공간의 구축은 기존의 문법화 연구 성과와 불가분의 관계에 있으며, 따라서 의미지도모형과 문법화 두 연구 체계는 상호보완과 상호이익의 양 측면이 공존하지만 대체로 전자가 후자로부터 이익을 취하는 경우가 그 반대의 경우보다 우세함을 증명하였다. This paper argues that there are three ways in which a conceptual space can be dynamicized, namely, (i)cross-linguistic comparison based on functional implication relationship, (ii)utilization of grammaticalization paths(semantic change included), and (iii)synchronic reconstruction by principles and degrees of grammaticalization. The paper also demonstrates that the building of a diachronic conceptual space depends heavily on the fruits of grammaticalization research.