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Doping Effect on the Metal-induced Lateral Crystallization Rate
Gui Fu Yang,Yong Woo Lee,Chang Woo Byun,Se Wan Son,주승기 대한금속·재료학회 2012 ELECTRONIC MATERIALS LETTERS Vol.8 No.2
The effects of phosphorus doping and boron doping on the metal-induced lateral crystallization (MILC) rate have been studied. In the case of phosphorus doping, the MILC rate is very sensitive to the sequence of nickel deposition and doping. Phosphorous doping before the nickel deposition reduced the MILC rate much more than the vice versa. When the nickel removal was between the nickel deposition and phosphorus doping,the doping effect on the MILC rate turned out to be in the between of the two cases which are phosphorus doping before nickel deposition and nickel deposition before phosphorus doping. Boron doping results in the crystallization of a-Si at a lower temperature (550°C) compared with that of the solid phase crystallization (SPC). The sheet resistance of boron doped Si remained high because the boron dopants were segregated at the grain boundaries during crystallization of a-Si.
Fu-Huang Lin,Minghan Luo,Longzhe Cui,Gui-Ping Wu 한국화학공학회 2015 Korean Journal of Chemical Engineering Vol.32 No.2
Activated carbon(AC) was prepared using brewing yeast as a precursor by chemical activation; meanwhile,cobalt, copper, ferrum and manganese supported on activated carbon(Co/AC, Cu/AC, Fe/AC, Mn/AC) were preparedby adsorption-activation method. The characterizations of prepared AC, Co/AC, Cu/AC, Fe/AC, Mn/AC and theirperformance as ozonation catalysts were tested. The total BET surface areas of prepared AC, Co/AC, Cu/AC, Fe/AC,Mn/AC were found to be 957.7, 789.7, 485.3, 486.1 and 529.8m2/g. Absorption capacities of methylene blue (MB) weredetermined to 407.77, 206.52, 121.25, 123.01, 170.94mg/g, respectively. The presence of AC was advantageous for TOCreduction of MB compared with UV/O3 system, and metal Co, Cu, Fe, Mn play an important role in the degradationprocess. The greatest TOC removal efficiency was obtained in the presence of Co/AC.
Fu-Wei Zhao,Min Luo,Yue-Hu Wang,Ma-Lin Li,Gui-Hua Tang,Chun-Lin Long 대한약학회 2010 Archives of Pharmacal Research Vol.33 No.11
A new piperidine alkaloid and three known tetranortriterpenoids were isolated from the methanol extracts of the rhizomes of Arisaema decipiens Schott (Araceae) and their chemical structures were identified as (-)-(2R*,3S*,6S*)-N,2-dimethyl-3-hydroxy-6-(9-phenylnonyl) piperidine (1), 6-deacetylnimbin (2), 28-deoxonimbolide (3) and nimbin (4). The N-methylated derivative (1a) of 1 was synthesized. Compound 1 exhibited weak inhibitory activity against the MCF-7 cell line, while compound 1a showed potential inhibitory activity against the MCF-7 cell line with an IC50 value of 4.6 μM and weak inhibitory activity against K562 and SK-OV-3 cells. This plant in genus Arisaema is firstly reported as the source of limonoids that are considered a natural antitumor herbal medicine.
Wen-Fu Zhang,Ying-Chun Liu,Gui-Lan Hou,Ke-Shan Chen,Jing Ji,Yun Deng,Shi-Lin Deng 한국강구조학회 2016 International Journal of Steel Structures Vol.16 No.4
The studies on the lateral-torsional buckling of cantilever steel beam with tip lateral elastic brace are rarely reported. The total potential energy is first established for the lateral-torsional buckling of cantilever steel beam with tip lateral elastic brace under uniform and concentrated load. The modal trial function of the lateral displacement and torsional angle are expressed as trigonometric function combination with six terms. By introducing new dimensionless parameters, the analytical solution of the dimensionless buckling equation for the lateral-torsional buckling of cantilever steel beam with tip lateral elastic brace is obtained. With the help of 1stOpt software which is mathematical optimization analysis software, the non-dimensional critical moment formula of the lateral-torsional buckling of cantilever beam with tip lateral elastic brace is obtained. Then, the accuracy of the formula is verified by ADINA finite element software. The results show that the given critical moment formula is of high accuracy. It provides convenient and simple design method for practical engineering.
Han, Gui Hwan,Seong, Wonjae,Fu, Yaoyao,Yoon, Paul K.,Kim, Seong Keun,Yeom, Soo-Jin,Lee, Dae-Hee,Lee, Seung-Goo Academic Press 2017 Metabolic engineering Vol.40 No.-
<P><B>Abstract</B></P> <P>Metabolons in nature have evolved to facilitate more efficient catalysis of multistep reactions through the co-localization of functionally related enzymes to cellular organelles or membrane structures. To mimic the natural metabolon architecture, we present a novel artificial metabolon that was created by targeting multi-enzyme cascade reactions onto inclusion body (IB) in <I>Escherichia coli</I>. The utility of this system was examined by co-localizing four heterologous enzymes of the 1-butanol pathway onto an IB that was formed in <I>E. coli</I> through overexpression of the cellulose binding domain (CBD) of <I>Cellulomonas fimi</I> exoglucanase. To target the 1-butanol pathway enzymes to the CBD IB, we utilized a peptide-peptide interaction between leucine zipper (LZ) peptides. We genetically fused the LZ peptide to the N-termini of four heterologous genes involved in the synthetic 1-butanol pathway, whereas an antiparallel LZ peptide was fused to the CBD gene. The <I>in vivo</I> activity of the CBD IB-based metabolon was examined through the determination of 1-butanol synthesis using <I>E. coli</I> transformed with two plasmids containing the LZ-fused CBD and LZ-fused 1-butanol pathway genes, respectively. <I>In vivo</I> synthesis of 1-butanol using the engineered <I>E. coli</I> yielded 1.98g/L of 1-butanol from glucose, representing a 1.5-fold increase over that obtained from <I>E. coli</I> expressing the LZ-fused 1-butanol pathway genes alone. In an attempt to examine the <I>in vitro</I> 1-butanol productivity, we reconstituted CBD IB-based metabolon using CBD IB and individual enzymes of 1-butanol pathway. The 1-butanol productivity of <I>in vitro</I> reconstituted CBD IB-based metabolon using acetoacetyl-CoA as the starting material was 2.29mg/L/h, 7.9-fold higher than that obtained from metabolon-free enzymes of 1-butanol pathway. Therefore, this novel CBD-based artificial metabolon may prove useful in metabolic engineering both <I>in vivo</I> and <I>in vitro</I> for the efficient production of desired products.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Enzyme cascades via heterologous metabolons facilitate host cell product production </LI> <LI> We used leucine zipper (LZ) tags to target metabolon reactions onto inclusion body </LI> <LI> Enhanced production of 1-butanol occurred both <I>in vitro</I> and <I>in vivo</I> </LI> <LI> Enzyme localization via LZ binding gave 7.9-fold increase in 1-butanol productivity </LI> <LI> This strategy may be useful in metabolic engineering for multiple target production </LI> </UL> </P>