Interleukin-17A production in Riemerella anatipestifer infection is independent to IL-23

Flores Rochelle(Flores Rochelle ),Fernandez Cherry(Fernandez Cherry ),Afrin Fahmida(Afrin Fahmida ),Cammayo Paula Leona(Cammayo Paula Leona ),Roy Anindita(Roy Anindita ),Min Wongi(Min Wongi ) 한국예방수의학회 2019 한국예방수의학회 학술대회자료집 Vol.2018 No.-

Molecular Characterization of Protease Producing Idiomarina Species Isolated from Peruvian Saline Environments

Flores-Fernandez, Carol N.,Chavez-Hidalgo, Elizabeth,Santos, Marco,Zavaleta, Amparo I.,Arahal, David R. The Korean Society for Microbiology and Biotechnol 2019 한국미생물·생명공학회지 Vol.47 No.3

All Idiomarina species are isolated from saline environments; microorganisms in such extreme habitats develop metabolic adaptations and can produce compounds such as proteases with an industrial potential. ARDRA and 16S rRNA gene sequencing are established methods for performing phylogenetic analysis and taxonomic identification. However, 16S-23S ITS is more variable than the 16S rRNA gene within a genus, and is therefore, used as a marker to achieve a more precise identification. In this study, ten protease producing Idiomarina strains isolated from the Peruvian salterns were characterized using biochemical and molecular methods to determine their bacterial diversity and industrial potential. In addition, comparison between the length and nucleotide sequences of a 16S-23S ITS region allowed us to assess the inter and intraspecies variability. Based on the 16S rRNA gene, two species of Idiomarina were identified (I. zobellii and I. fontislapidosi). However, biochemical tests revealed that there were differences between the strains of the same species. Moreover, it was found that the ITS contains two tRNA genes, $tRNA^{Ile(GAT)}$ and $tRNA^{Ala(TGC)}$, which are separated by an ISR of a variable size between strains of I. zobellii. In one strain of I. zobellii (PM21), we found nonconserved nucleotides that were previously not reported in the $tRNA^{Ala}$ gene sequences of Idiomarina spp. Thus, based on the biochemical and molecular characteristics, we can conclude that protease producing Idiomarina strains have industrial potential; only two I. zobellii strains (PM48 and PM72) exhibited the same properties. The differences between the other strains could be explained by the presence of subspecies.

International comparison CCQM-K82: methane in air at ambient level (1800 to 2200) nmol/mol

Flores, Edgar,Viallon, Joë,le,Choteau, Tiphaine,Moussay, Philippe,I Wielgosz, Robert,Kang, Namgoo,Moon Kim, Byung,Zalewska, Ewelina,(A M H) van der Veen, Adriaan,Konopelko, Leonid,Wu, Hai,Han, Qia IOP 2015 Metrologia Vol.52 No.-

<P>The CCQM-K82 comparison was designed to evaluate the degrees of equivalence of NMI capabilities for methane in air primary reference mixtures in the range (1800 to 2200) nmol/mol. The balance gas for the standards was either scrubbed dry real air or synthetic air.</P> <P> CH<SUB>4</SUB> in air standards have been produced by a number of laboratories for many years, with more recent developments focused on standards at atmospheric measurement concentrations and aimed at obtaining agreement between independently produced standards. A comparison of the differences in primary gas standards for methane in air was previously performed in 2003 (CCQM-P41 Greenhouse gases. 1 and 2) with a standard deviation of results around the reference value of 30 nmol/mol and 10 nmol/mol for a more limited set of standards. This can be contrasted with the level of agreement required from field laboratories routinely measuring atmospheric methane levels, set by Data Quality Objectives (DQO) established by the World Meteorological Organization (WMO) to reflect the scientifically desirable level of compatibility for CH<SUB>4</SUB> measurements at the global scale, currently set at 2 nmol/mol (1 sigma).</P> <P> The measurements of this key comparison took place from May 2012 to June 2012.</P> <P> Eight laboratories took part in this comparison coordinated by the BIPM and NIST. Key comparison reference values were calculated based on Cavity Ring Down Spectroscopy Measurements performed at the BIPM, combined with participant's gravimetric values to identify a consistent set of standards. Regression analysis allowed predicted values for each standard to be calculated which acted as the KCRVs. In this comparison reported standard uncertainties by participants ranged from 0.50 nmol/mol to 2.4 nmol/mol and the uncertainties of individual KCRVs ranged from 0.68 nmol/mol to 0.71 nmol/mol.</P> <P> The standard deviation of the ensemble of standards about the KCRV value was 1.70 nmol/mol. This represents a greater than tenfold improvement in the level of compatibility of methane in air standards compared to that demonstrated in 2003. Further improvements in the compatibility of standards will require improved methods and uncertainties for the measurement of trace level methane in balance gases. </P> <P><B>Main text.</B> To reach the main text of this paper, click on <A HREF='http://www.bipm.org/utils/common/pdf/final_reports/QM/K82/CCQM-K82.pdf'>Final Report</A>. Note that this text is that which appears in Appendix B of the BIPM key comparison database <A HREF='http://kcdb.bipm.org/'>kcdb.bipm.org/</A>.</P> <P>The final report has been peer-reviewed and approved for publication by CCQM, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).</P>

B lymphocytes taken to task: a role for a background conductance K2P K+ channel in B cells. Focus on 'Expression of TASK-2 and its upregulation by B cell receptor stimulation in WEHI-231 mouse immature B cells'.

Flores, Carlos A,Cid, L Pablo,Niemeyer, Marí,a Isabel,Sepú,lveda, Francisco V American Physiological Society 2011 American journal of physiology. Cell physiology Vol.300 No.5

Final report on international comparison CCQM-K74: Nitrogen dioxide, 10 µmol/mol

Flores, Edgar,Idrees, Faraz,Moussay, Philippe,Viallon, Joë,le,Wielgosz, Robert,Ferná,ndez, Teresa,Ramí,rez, Sergio,Rojo, André,s,Shinji, Uehara,Waldé,n, Jari,Sega, Michela Springer-Verlag 2012 METROLOGIA -BERLIN- Vol.49 No.-

<P>There is a high international priority attached to activities which reduce NO<SUB>x</SUB> in the atmosphere. The current level of permitted emissions is typically between 50 µmol/mol and 100 µmol/mol, but lower values are expected in the future. Currently, ambient air quality monitoring regulations also require the measurement of NO<SUB>x</SUB> mole fractions as low as 0.2 µmol/mol. The production of accurate standards at these levels of mole fractions requires either dilution of a stable higher concentration gas standard or production by a dynamic technique, for example one based on permeation tubes.</P><P>The CCQM-K74 key comparison was designed to evaluate the level of comparability of National Metrology Institutes' measurement capabilities and standards for nitrogen dioxide (NO<SUB>2</SUB>) at a nominal mole fraction of 10 µmol/mol.</P><P>The measurements of this key comparison took place from June 2009 to May 2010.</P><P>Seventeen laboratories took part in this comparison coordinated by the BIPM and VSL. The key comparison reference value was based on BIPM measurement results, and the standard measurement uncertainty of the reference value was 0.042 µmol/mol.</P><P>This key comparison demonstrated that the results of the majority of the participants agreed within limits of ±3% relative to the reference value. The results of only one laboratory lay significantly outside these limits. Likewise this comparison made clear that a full interpretation of the results of the comparison needed to take into account the presence of nitric acid (in the range 100 nmol/mol to 350 nmol/mol) in the cylinders circulated as part of the comparison, as well as the possible presence of nitric acid in the primary standards used by participating laboratories.</P><P>Main text.To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/.</P><P>The final report has been peer-reviewed and approved for publication by the CCQM, according to the provisions of the CIPM Mutual Recognition Arrangement (MRA).</P>

CCQM-K120 (Carbon dioxide at background and urban level)

Flores, Edgar,Viallon, Joë,le,Choteau, Tiphaine,Moussay, Philippe,Idrees, Faraz,Wielgosz, Robert I,Lee, Jeongsoon,Zalewska, Ewelina,Nieuwenkamp, Gerard,Veen, Adriaan van der,Konopelko, L A,Kustiko BUREAU INTERNATIONAL DES POIDS ET MESURES 2019 METROLOGIA -BERLIN- Vol.56 No.-

<P></P> <P>CCQM-K120.a comparison involves preparing standards of carbon dioxide in air which are fit for purpose for the atmospheric monitoring community, with stringent requirements on matrix composition and measurement uncertainty of the CO<SUB>2</SUB> mole fraction. This represents an analytical challenge and is therefore considered as a Track C comparison. The comparison will underpin CMC claims for CO<SUB>2</SUB> in air for standards and calibrations services for the atmospheric monitoring community, matrix matched to real air, over the mole fraction range of 250 μmol/mol to 520 μmol/mol.</P> <P>CCQM-K120.b comparison tests core skills and competencies required in gravimetric preparation, analytical certification and purity analysis. It is considered as a Track A comparison. It will underpin CO<SUB>2</SUB> in air and nitrogen claims in a mole fraction range starting at the smallest participant's reported expanded uncertainty and ending at 500 mmol/mol. Participants successful in this comparison may use their result in the flexible scheme and underpin claims for all core mixtures</P> <P>This study has involved a comparison at the BIPM of a suite of 44 gas standards prepared by each of the participating laboratories. Fourteen laboratories took part in both comparisons (CCQM-K120.a, CCQM-K120.b) and just one solely in the CCQM-K120.b comparison.</P> <P>The standards were sent to the BIPM where the comparison measurements were performed. Two measurement methods were used to compare the standards, to ensure no measurement method dependant bias: GC-FID and FTIR spectroscopic analysis corrected for isotopic variation in the CO<SUB>2</SUB> gases, measured at the BIPM using absorption laser spectroscopy. Following the advice of the CCQM Gas Analysis Working Group, results from the FTIR method were used to calculate the key comparison reference values.</P> <P>KEY WORDS FOR SEARCH</P> <P>FTIR, CO2, GC-FID, Carbon dioxide at background level, Carbon dioxide at urban level, Delta Ray, CO2 gas standards</P> <P></P> <H2>Main text</H2> <P> To reach the main text of this paper, click on <A HREF='https://www.bipm.org/utils/common/pdf/final_reports/QM/K120/CCQM-K120.pdf'>Final Report</A>. Note that this text is that which appears in Appendix B of the BIPM key comparison database <A HREF='http://kcdb.bipm.org/'>kcdb.bipm.org/</A>.</P> <P>The final report has been peer-reviewed and approved for publication by the CCQM, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).</P>

Interior Spatial Compositions of Vernacular Dwellings in the Philippines

Flores Thelma Lazo(フロ?レス テルマ ラソ),Miyazaki Kiyoshi(宮崎 ?) 한국디자인학회 1997 디자인학연구 Vol.- No.22

Final report of the pilot study CCQM-P110-B1: A comparison of nitrogen dioxide (NO₂) in nitrogen standards at 10 µmol/mol by Fourier transform infrared spectroscopy (FT-IR)

Flores, Edgar,Idrees, Faraz,Moussay, Philippe,Viallon, Joë,le,Wielgosz, Robert,Ferná,ndez, Teresa,Rojo, André,s,Ramí,rez, Sergio,Aoki, Nobuyuki,Kato, Kenji,Jeongsoon, Lee,Moon, D Springer-Verlag 2012 METROLOGIA -BERLIN- Vol.49 No.-

<P>This pilot study compares the performance of participants in analyzing gas mixtures of nitrogen dioxide in nitrogen by comparison with in-house gravimetric standards using Fourier transformed infrared spectroscopy (FT-IR). In this study the same gas mixtures were used as in the key comparison CCQM-K74, which was designed to evaluate the level of comparability of National Metrology Institutes' measurement capabilities for nitrogen dioxide (NO<SUB>2</SUB>) at a nominal mole fraction of 10 µmol/mol. In the comparison CCQM-K74 most of the participants used chemiluminescence, with a small number using UV absorption or FT-IR spectroscopy, and thus it is of interest to improve understanding of the comparative performance of these techniques because they do not exhibit any cross-sensitivity to nitric acid (HNO<SUB>3</SUB>), which was known to be present in the mixtures used for the comparison.</P><P>The results of this pilot study indicate good consistency and a level of agreement similar to that reported in the comparison CCQM-K74, demonstrating that FT-IR can be operated as a comparison method when calibrated with appropriate gas standards and can achieve similar measurement uncertainties to chemiluminescence and UV absorption techniques.</P><P>An additional pilot study, CCQM-P110-B2, was conducted on the same gas mixtures in parallel with this pilot study. The second study addressed FT-IR spectroscopy when used to measure the gas mixtures with respect to reference spectra. The results of this second study will be reported elsewhere.</P><P>Main text.To reach the main text of this paper, click on Final Report.</P><P>The final report has been peer-reviewed and approved for publication by the CCQM-GAWG.</P>

Comparative Spatial Analysis Between Inner-City Socialized Housing and Private Housing Developments in Metro Manila, the Philippines

Flores, Diane Angeline,Jang, Seongman,Lee, Seungil Land and Housing Institute 2021 LHI journal of land, housing, and urban affairs Vol.12 No.2

필리핀 마닐라의 급속한 도시화는 유래 없는 인구성장을 가져왔고, 이는 국가 전체에 이중적 주택문제 즉 공영주택의 공실과 부족 현상을 동시에 초래하였다. 이 연구의 목적은 GIS 기반의 2개 통계모형을 개발하여 접근성, 사회·경제요인, 환경재난의 취약성 등 사이의 관계성을 기준으로 일반주택과 비교하여 공영주택의 공간분포 특성을 파악하는데 있다. 이 연구에서는 공간분석을 위해 다중회귀모형과 GIS를 연계시켜 공영주택과 일반주택의 공간분포에 영향을 미치는 주요 변수를 각각 확인하였다. 2개 회귀모형의 분석결과를 비교하였더니, 공영주택은 부적절한 토지이용에 교통과 도시지원시설에의 접근성이 열악한 곳에 분포하여 입지하고 있음을 확인하였다. 나아가 2개의 모형 모두 환경재난 취약성을 매우 중요하게 설명하였다. 이는 현재 주택정책이 국가의 주택 위기상황, 특히 소외된 저소득층 가구의 주택문제를 해소하지 못하는 이유를 밝히고 있다. 그러므로 이들 모형의 적용결과는 도시계획가와 지자체 의사결정자로 하여금 주택부문의 발전을 목적으로 수행 중인 현재의 정책 개입을 혁신적으로 개선해야 함을 시사점으로 제공한다. Rapid urbanization has resulted in the unprecedented growth of population in Metro Manila, the Philippines and has led to a 'dual' housing crisis - vacant/unoccupied socialized housing and a chronic housing shortage or delayed housing supply. By developing two GIS-based statistical models, this study is to examine socialized housing in comparison with private housing with respect to location patterns, integration, accessibility, social and economic aspects, and vulnerability to environmental hazards. Multiple regression analysis was integrated with the GIS to identify significant variables that influence the spatial distribution of socialized housing. The comparison between the two regression models has shown that socialized housing is located in areas with inappropriate land use and poor accessibility to transportation facilities and built urban resources. Moreover, both regression models have proven the statistical significance of the vulnerability of socialized housing to environmental hazards. The finding explains how the current housing policies do not address the country's housing crisis, especially for the marginalized and low-income households. Thus, the findings provide implications for urban planners and local decision-makers in reforming the current policy interventions.

Simulation of Pavement's Dynamic Response Under the Rolling Dynamic Deflectometer Loading Using 3D Finite Element Modeling

Flores Julius Marvin,Hyun Jong Lee,Carlo Elipse,Hee Mun Park 한국도로학회 2019 한국도로학회 학술대회 발표논문 초록집 Vol.2019 No.09