Xanthomonas axonopodis pv. eucalyptorum pv. nov. Causing Bacterial Leaf Blight on Eucalypt in Brazil

Ferraz, Helvio Gledson Maciel,Badel, Jorge Luis,da Silva Guimaraes, Lucio Mauro,Reis, Bruna Paolinelli,Totola, Marcos Rogerio,Goncalves, Rivadalve Coelho,Alfenas, Acelino Couto The Korean Society of Plant Pathology 2018 Plant Pathology Journal Vol.34 No.4

Bacterial leaf blight is a major disease of eucalypt, especially under nursery conditions. Different bacterial species have been associated with the disease in several countries, and despite its importance worldwide, it is not clear to date whether similar disease symptoms are caused by the same or by different etiological agents. In this study, 43 bacterial strains were isolated from blighted eucalypt leaves collected in different geographic areas of Brazil and inoculated onto a susceptible eucalypt clone. Polyphasic taxonomy, including morphological, physiological, biochemical, molecular, and pathogenicity tests showed that only certain strains of Xanthomonas axonopodis caused symptoms of the disease. Strains varied in their aggressiveness, but no correlation with geographic origin was observed. MLSA-based phylogenetic analysis using concatenated dnaK, fyuA, gyrB and rpoD gene sequences allocated the strains in a well-defined clade, corresponding to Rademarker's group RG 9.6. Inoculation of nineteen plant species belonging to seven botanical families with representative strain LPF 602 showed it to be pathogenic only on Eucalyptus spp, and Corymbia spp. Based on distinct biochemical and pathogenic characteristics that differentiate the eucalypt strains from other pathovars of the X. axonopodis species, here we propose their allocation into the new pathovar X. axonopodis pv. eucalyptorum pv. nov.

Xanthomonas axonopodis pv. eucalyptorum pv. nov. Causing Bacterial Leaf Blight on Eucalypt in Brazil

Hélvio Gledson Maciel Ferraz,Jorge Luis Badel,Lúcio Mauro da Silva Guimarães,Bruna Paolinelli Reis,Marcos Rogério Tótola,Rivadalve Coelho Gonçalves,Acelino Couto Alfenas 한국식물병리학회 2018 Plant Pathology Journal Vol.34 No.4

Bacterial leaf blight is a major disease of eucalypt, especially under nursery conditions. Different bacterial species have been associated with the disease in several countries, and despite its importance worldwide, it is not clear to date whether similar disease symptoms are caused by the same or by different etiological agents. In this study, 43 bacterial strains were isolated from blighted eucalypt leaves collected in different geographic areas of Brazil and inoculated onto a susceptible eucalypt clone. Polyphasic taxonomy, including morphological, physiological, biochemical, molecular, and pathogenicity tests showed that only certain strains of Xanthomonas axonopodis caused symptoms of the disease. Strains varied in their aggressiveness, but no correlation with geographic origin was observed. MLSA-based phylogenetic analysis using concatenated dnaK, fyuA, gyrB and rpoD gene sequences allocated the strains in a well-defined clade, corresponding to Rademarker’s group RG 9.6. Inoculation of nineteen plant species belonging to seven botanical families with representative strain LPF 602 showed it to be pathogenic only on Eucalyptus spp, and Corymbia spp. Based on distinct biochemical and pathogenic characteristics that differentiate the eucalypt strains from other pathovars of the X. axonopodis species, here we propose their allocation into the new pathovar X. axonopodis pv. eucalyptorum pv. nov.

Chlorhexidine gel associated with papain in pulp tissue dissolution

Couto De Oliveira, Gabriel,Ferraz, Caio Souza,Andrade Junior, Carlos Vieira,Pithon, Matheus Melo The Korean Academy of Conservative Dentistry 2013 Restorative Dentistry & Endodontics Vol.38 No.4

Objectives: This study aimed to evaluate the capacity of 2% chlorhexidine gel associated with 8% papain gel in comparison with 5.25% sodium hypochlorite in bovine pulp tissue dissolution. Materials and Methods: Ninety bovine pulps of standardized sizes were used and fragmented into 5-mm sizes. The fragments were removed from the root middle third region. They were divided into 6 experimental groups (n = 15), 1) 8% papain; 2) 2% chlorhexidine; 3) 2% chlorhexidine associated with 8% papain; 4) 0.9% saline solution; 5) 2.5% sodium hypochlorite; and 6) 5.25% sodium hypochlorite. The pulp fragments were weighed and put into immobile test tubes for dissolution for time intervals of 30, 60, 90, and 120 min. Results: The 5.25% sodium hypochlorite had greater dissolution potential than the pure papain, and when associated with chlorhexidine, both promoted greater dissolution than did the saline solution and 2% chlorhexidine groups (p < 0.05). The 2.5% sodium hypochlorite promoted dissolution to a lesser extent than the groups with papain within a period of 30 min (p < 0.05), but, was comparable to the saline solution and chlorhexidine. After 120 min, the 2.5% and 5.25% sodium hypochlorite promoted dissolution of 100% of the pulp fragments, and papain, 61%, while chlorhexidine associated with papain and chlorhexidine alone dissolved only 55% and 3%, respectively. Conclusions: The 8% papain in gel, both alone and in association with chlorhexidine, was able to dissolve bovine pulp tissue, but to a lesser extent than did 5.25% sodium hypochlorite.

International Education, Qualification and Certification Systems in Welding

L., Quintino,R., Ferraz,I., Fernandes The Korean Welding and Joining Society 2007 대한용접·접합학회지 Vol.25 No.6

The International System for Education and Qualification of Welding Personnel has been implemented based on the harmonized European System for education and qualification of welding personnel. This paper gives an overview of the International System focusing on the training guidelines and the quality assurance system developed. Systems for harmonization of Certification of Welding Personnel and for supporting companies using welding to implement ISO 3834 have been developed by EWF and are presently being transferred to IIW in line with the EWF/IIW agreement established in 2000.

Substitution Materials for Sustainable Concrete Production in Portugal

Paulo Cachim,Ana Luisa Velosa,Eduardo Ferraz 대한토목학회 2014 KSCE Journal of Civil Engineering Vol.18 No.1

The development of technologies for environmental protection contributes to save and preserve natural resources. Recently, both government and private sectors have accepted the view that rational utilization of non-renewable resources must be achieved. Historically, concrete industry relies heavily on the use of natural aggregates and Portland cement. Abundance of natural fine and coarse aggregates, located close to major cities, prescription specifications, and large production and inexpensive transport have contributed to this practice. On the other hand, related with limited waste disposal sites, an ecologically sustainable and responsible attitude is being adopted with respect to waste minimisation and utilisation of alternative materials in construction, to improve sustainability through energy efficiency, reducing CO2 emissions, the cost of building and life-cycle maintenance. This paper presents the use of some natural raw materials and industrial by-products as substitution materials towards concrete sustainability. The reported materials are used as cement or aggregate replacement and are environmentally safe (non hazardous) and stable.

Maxillary first molar with 7 root canals diagnosed using cone-beam computed tomography

Rodrigues, Evaldo,Braitt, Antonio Henrique,Galvao, Bruno Ferraz,da Silva, Emmanuel Joao Nogueira Leal The Korean Academy of Conservative Dentistry 2017 Restorative Dentistry & Endodontics Vol.42 No.1

Root canal anatomy is complex, and the recognition of anatomic variations could be a challenge for clinicians. This case report describes the importance of cone beam computed tomographyic (CBCT) imaging during endodontic treatment. A 23 year old woman was referred by her general dental practitioner with the chief complaint of spontaneous pain in her right posterior maxilla. From the clinical and radiographic findings, a diagnosis of symptomatic irreversible pulpitis was made and endodontic treatment was suggested to the patient. The patient underwent CBCT examination, and CBCT scan slices revealed seven canals: three mesiobuccal (MB1, MB2, and MB3), two distobuccal (DB1 and DB2), and two palatal (P1 and P2). Canals were successfully treated with reciprocating files and filled using single-cone filling technique. Precise knowledge of root canal morphology and its variation is important during root canal treatment. CBCT examination is an excellent tool for identifying and managing these complex root canal systems.

Maxillary first molar with 7 root canals diagnosed using cone-beam computed tomography

Evaldo Rodrigues,Antônio Henrique Braitt,Bruno Ferraz Galvão,Emmanuel João Nogueira Leal da Silva 대한치과보존학회 2017 Restorative Dentistry & Endodontics Vol.42 No.1

Root canal anatomy is complex, and the recognition of anatomic variations could be a challenge for clinicians. This case report describes the importance of cone beam computed tomographyic (CBCT) imaging during endodontic treatment. A 23 year old woman was referred by her general dental practitioner with the chief complaint of spontaneous pain in her right posterior maxilla. From the clinical and radiographic findings, a diagnosis of symptomatic irreversible pulpitis was made and endodontic treatment was suggested to the patient. The patient underwent CBCT examination, and CBCT scan slices revealed seven canals: three mesiobuccal (MB1, MB2, and MB3), two distobuccal (DB1 and DB2), and two palatal (P1 and P2). Canals were successfully treated with reciprocating files and filled using single-cone filling technique. Precise knowledge of root canal morphology and its variation is important during root canal treatment. CBCT examination is an excellent tool for identifying and managing these complex root canal systems.

Tarsal switch using an anterior approach to correct severe ptosis

Meneghim, Roberta Lilian Fernandes de Sousa,Ferraz, Lucieni Barbarini,Galindo-Ferreiro, Alicia,Khandekar, Rajiv,Sanchez-Tocino, Hortensia,Schellini, Silvana Korean Society of Plastic and Reconstructive Surge 2018 Archives of Plastic Surgery Vol.45 No.2

Background To present the outcomes of the tarsal switch procedure using an anterior approach to correct severe ptosis with poor levator muscle function (<4 mm) with absent or poor Bell's phenomenon. Methods This retrospective case series included 11 patients with severe neurogenic or acquired myogenic palpebral ptosis. All patients underwent the tarsal switch procedure through an anterior approach from 2012 to 2015. Margin reflex distance (MRD1 and MRD2) and the palpebral fissure were evaluated preoperatively and postoperatively. Data were compared using the Wilcoxon signed-rank test. P-values <0.05 were considered to indicate statistical significance. Results Surgery was performed on 18 eyelids (11 patients). The median age at surgery was 57 years (range, 29-86 years). Four patients had unilateral ptosis and seven had bilateral ptosis. Nine patients had myogenic ptosis and two had neurogenic ptosis. Postoperatively, the chin-up position improved in all patients. The MRD1 increased statistically significantly, from 0 mm preoperatively to 1.0 mm postoperatively (P=0.001). The MRD2 decreased statistically significantly, from 4.5 mm preoperatively to 3.0 mm postoperatively (P=0.001). The palpebral fissure did not change (4.0 mm preoperatively to 4.0 mm postoperatively) (P=0.13). Conclusions The tarsal switch procedure through an anterior approach is an effective alternative for correcting severe ptosis, especially neurogenic or acquired myogenic ptosis. This procedure can be performed with minimal risk of ocular surface exposure and provides stable outcomes.

Production of omega 3, 6, and 9 fatty acids from hydrolysis of vegetable oils and animal fat with Colletotrichum gloeosporioides lipase

Denise Sande,Gecernir Colen,Gabriel Franco dos Santos,Vany Perpe´tua Ferraz,Jacqueline Aparecida Takahashi 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.2

Hydrolysis of vegetable oils (Olive, corn, peanut, sesame, flaxseed, soy, canola, garlic, sunflower, almond, castor bean oils) and beef marrow bone oil by Colletotrichum gloeosporioides lipase was studied. The enzyme was capable of generating free fatty acids from all oils tested. The higher hydrolytic activity of the enzyme was towards olive (18.0 IU) and soybean (17.8 IU) oils. The average percentage of essential fatty acids generated from hydrolysis of the oils was 32.92% of omega 9 (as oleic acid C18:1), 26.24% of omega 6 (linoleic C18:2), and 5.86% of omega 3 (such as a-linolenic acid C18:3). Comparison between chromatographic profile of the oils and its enzymatic hydrolysate showed a good equivalence, stressing the applicability of these vegetable substrates under the action of lipase from C. gloeosporioides produce essential fatty acids, being more efficient production of alinolenic acid from flaxseed oil, linoleic acid from sunflower oil, and oleic acid from olive.

State of the Art of Biodegradable Nanofluids Application in Machining Processes

Vitor Baldin,Leonardo Rosa Ribeiro da Silva,Alisson Rocha Machado,Celso Ferraz Houck 한국정밀공학회 2023 International Journal of Precision Engineering and Vol.10 No.5

For ecological and health issues of those involved in the manufacturing, usage, handling, and disposal of cutting fluids, research has been developed for scientific and technological advances in biodegradable cutting fluids, application methods, and solid nanolubricants. Using a minimum quantity of lubricant (MQL) in machining processes proved to be a viable alternative to replacing low-pressure jet machining. Nanoparticles from 5 to 100 nm in size are usually dispersed in vegetable-based cutting fluid; this combination improves the tribological properties of the nanofluid and the machinability of metal alloys like steel, titanium, nickel, and aluminum alloys, with reduced cutting forces, cutting temperature, tool wear and workpiece surface roughness. This work aims to present an updated summary of the lubrication and cooling action provided by using the MQL technique, biodegradable cutting fluids applied by MQL, the use of nanoparticles added to the cutting fluids, and the physical properties of nanoparticles, tribological characteristics, and the behavior of the nanofluids. The changes in machining force, cutting temperature, surface integrity, and wear of cutting tools with biodegradable nano-cutting fluids are also focused on. The use of nanoparticles in cutting fluids associated with MQL application has shown an increase in the lubricating and coolant properties of cutting fluids, which contribute to the reduction of machining force, cutting temperature, workpiece surface roughness, coefficient of friction, and wear of cutting tools. Thus, the results of this summary can provide theoretical support and experimental guidance for exploring the lubricating and cooling properties and the mechanism present in film formation with nanofluids at the chip-too-workpiece interfaces. Knowledge of such phenomena helps to popularize an eco-friendly practice in metal-mechanic industries.