Atmospheric Pressure Air Plasma Treatment to Improve Dyeability with Cortex Phellodendri Plant Powder Dye: An Ecofriendly and Energy-Efficient Approach for Silk Processing

Fei Fan,Xunxin Wu,Jiaxiang Wen,Gengyu Lin,Jiawen Lin,Qiuting Yin 한국섬유공학회 2023 Fibers and polymers Vol.24 No.10

This paper explores the effects of a dielectric barrier discharge atmospheric pressure air plasma and citric acid (CA) pretreatment on dyeing gray mulberry silk plain fabrics with a cortex phellodendri plant powder dyestuff. The atmospheric air plasma treatment formed new hydrophilic groups, such as C-O, O-C=O, and C-NH2, on the surface of the fabric. The relative contents of the C-O (C-N) and O-C=O groups indicated that the efficiency of bonding the hydrophilic groups to the fabric increased in the order of citric acid alone < collaborative treatment in different orders < original < atmospheric air plasma alone. X-ray photoelectron spectroscopy (XPS) showed that the relative content of C-O (C-N)/C=O was the highest after 9 treatments at 360 W. Erosion pits and grooves with widths of 0.5-3 µm appeared on the surface after plasma treatment. The surface of the silk fabric was negatively charged, and it interacted electrostatically, adsorbed and combined with the cationic dye. The samples were dyed at 60 °C, with a pH 8, a 60% (owf) dye concentration, a bath ratio of 1:40, and a 60 min dyeing time. After soaping, the K/S value had increased from 8.05 to 13.51, and the washing color fastness rating was 4-5.

Mathematical Simulation of Spray Forming Process

( Jun Fei Fan ),( Yin Zhang ),( San Bing Ren ),( You Duo He ),( Hai Rong Le ) 한국액체미립화학회 2005 한국액체미립화학회 학술강연회 논문집 Vol.2005 No.-

In this paper, an integrated approach for modeling the entire spray forming process is presented and the program for the simulation is written by hand. The whole spray forming process can be divided into four calculate process and the basis for the analysis is classical k-ε turbulence model which used to simulate the flow field of gas formed in the chamber. The other three mathematical models are atomization model,deposition model and solidification model. In the atomization model coupled the flow field of gas and formation、velocity and location of droplet. By means of integrate above mathematical model the process of spraying forming was simulated in detail.

Identification of novel rheumatoid arthritis-associated MiRNA-204-5p from plasma exosomes

Wu Long-Fei,Zhang Qin,Mo Xing-Bo,Lin Jun,Wu Yang-Lin,Lu Xin,He Pei,Wu Jian,Guo Yu-Fan,Wang Ming-Jun,Ren Wen-Yan,Deng Hong-Wen,Lei Shu-Feng,Deng Fei-Yan 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Rheumatoid arthritis (RA) is an autoimmune disease characterized by infiltration of immune cells in the synovium. However, the crosstalk of immune cells and synovial fibroblasts is still largely unknown. Here, global miRNA screening in plasma exosomes was carried out with a custom microarray (RA patients vs. healthy controls = 9:9). A total of 14 exosomal miRNAs were abnormally expressed in the RA patients. Then, downregulated expression of exosomal miR-204-5p was confirmed in both the replication (RA patients vs. healthy controls = 30:30) and validation groups (RA patients vs. healthy controls = 56:60). Similar to the findings obtained in humans, a decreased abundance of exosomal miR-204-5p was observed in mice with collagen-induced arthritis (CIA). Furthermore, Spearman correlation analysis indicated that plasma exosomal miR-204-5p expression was inversely correlated with disease parameters of RA patients, such as rheumatoid factor, erythrocyte sedimentation rate, and C-reactive protein. In vitro, our data showed that human T lymphocytes released exosomes containing large amounts of miR-204-5p, which can be transferred into synovial fibroblasts, inhibiting cell proliferation. Overexpression of miR-204-5p in synovial fibroblasts suppressed synovial fibroblast activation by targeting genes related to cell proliferation and invasion. In vivo assays found that administration of lentiviruses expressing miR-204-5p markedly alleviated the disease progression of the mice with CIA. Collectively, this study identified a novel RA-associated plasma exosomal miRNA-204-5p that mediates the communication between immune cells and synovial fibroblasts and can be used as a potential biomarker for RA diagnosis and treatment.

Psychopathological Profile of Women with Breast Cancer Based on the Symptom Checklist-90-R

Pan, Xiong-Fei,Fei, Man-Dong,Zhang, Kenneth Y.,Fan, Zhen-Lie,Fu, Feng-Huan,Fan, Jin-Hu Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11

Background: With effective early treatments, many breast cancer patients suffer from psychological distress due to adverse effects and lifelong physical disfigurement. Our study aimed to evaluate the psychopathological profile of breast cancer patients in comparison with healthy women and explored demographic correlates. Method: We consecutively enrolled breast cancer patients who came to the hospital for follow-up or rehabilitation care after primary treatment, and healthy female relatives or friends of inpatients in the Cancer Institute of Chinese Academy of Medical Sciences between August 30, 2010 and January 1, 2012. Psychopathological profile was assessed based on the Symptom Checklist-90-R (SCL-90-R) for patients and controls. We compared demographics such as age, ethnicity, education, marriage, and occupation, and incorporated these data plus cancer status for the association with the general SCL-90-R index and scores for 9 major symptom dimensions in multiple regression analysis. Results: We surveyed a total of 291 female breast cancer patients and 531 healthy women. The average age was $55.1{\pm}6.40$ years for breast cancer patients and $43.1{\pm}12.8$ for healthy controls (P<0.01). The mean survival was 5.20 years for cancer patients (range, 0.60-9.90 years). There were statistically significant differences in education, marriage, and occupation between the two groups (P<0.01). General index ($1.45{\pm}0.45$ versus $1.32{\pm}0.37$) and 8 dimension scores (excluding anxiety) on SCL-90-R were significantly higher in patients (P<0.05). Multiple regression analysis showed that the breast cancer status was positively correlated with general SCL-90-R index and 6 dimension scores (excluding the anxiety, phobic anxiety and paranoid ideation dimensions) (P<0.05). Regression coefficients ranged from 0.10 (depression) to 0.19 (somatization). Higher interpersonal sensitivity was noticed in single women compared to married women. Conclusions: Chinese patients with breast cancer demonstrate greater psychopathology compared to healthy controls. The breast cancer status is an independent contributing factor to the general psychopathological profile. Breast cancer patients should be given particular counseling and care to alleviate their psychological distress.

The Combined Effect of Sodium Butyrate and Low Culture Temperature on the Production, Sialylation, and Biological Activity of an Antibody Produced in CHO Cells

Fei Chen,Tianci Kou,Li Fan,Yan Zhou,Zhaoyang Ye,Liang Zhao,Wen-Song Tan 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.6

Cell cultures containing 0 ~ 5 mM sodium butyrate (NaBu) and grown at 30 and 37°C were conducted to investigate the combined effect of NaBu and low temperature on the quantity and quality of an antibody production in CHO cells. Although NaBu addition decreased cell viability by apoptosis in a dose-dependent manner at both 30 and 37°C, the onset of significant apoptosis induced by NaBu was delayed by lowering culture temperature. The highest specific antibody productivity (q_p) of 23.26 pg/cell/day was obtained in the culture containing 2 mM NaBu at 30°C; however, the highest antibody concentration of 167.84 mg/L was achieved in the culture containing 1 mM NaBu at 30°C, as the detrimental effect of further NaBu addition on cell growth compromised its beneficial effect on q_p. Moreover, protein quality with respect to the total sialic acid content and Nglycolylneuraminic acid (Neu5Gc) level was evaluated. There were no apparent changes regarding the total sialic acid content of the antibody, but manipulation of cultures with NaBu treatment or (and) low culture temperature did decrease Neu5Gc levels by 5 ~ 10%. Biological activity of the antibody was also assessed, and no obvious changes were observed. Collectively, the simultaneous application of NaBu and low culture temperature was an effective way to extend culture period and enhance final antibody concentration,without compromising the sialic acid content or biological activity.

A Novel All-trans Retinoid Acid Derivative N-(3-trifluoromethyl-phenyl)-Retinamide Inhibits Lung Adenocarcinoma A549 Cell Migration through Down-regulating Expression of Myosin Light Chain Kinase

Fan, Ting-Ting,Cheng, Ying,Wang, Yin-Feng,Gui, Shu-Yu,Chen, Fei-Hu,Zhou, Qing,Wang, Yuan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.18

Aim: To observe the effects of a novel all-trans retinoid acid (ATRA) derivative, N-(3-trifluoromethyl-phenyl)-retinamide (ATPR), on lung adenocarcinoma A549 cells and to explore the potential mechanism of ATPR inhibiting of A549 cell migration. Materials and Methods: The cytotoxicity of ATRA and ATPR on A549 cells was assessed using MTT assay. Wound healing assays were used to analyze the influences of ATRA, ATPR, ML-7 (a highly selective inhibitor of myosin light chain kinase (MLCK)), PMA (an activator of MAPKs) and PD98059 (a selective inhibitor of ERK1/2) on the migration of A549 cells. Expression of MLCK and phosphorylation of myosin light chain (MLC) were assessed by Western blotting. Results: ATRA and ATPR inhibited the proliferation of A549 cells in a dose- and time-dependent manner, and the effect of ATPR was much more remarkable compared with ATRA. Relative migration rate and migration distance of A549 cells both decreased significantly after treatment with ATPR or ML-7. The effect on cell migration of PD98059 combining ATPR treatment was more notable than that of ATPR alone. Moreover, compared with control groups, the expression levels of MLCK and phosphorylated MLC in A549 cells were both clearly reduced in ATRA and ATPR groups. Conclusions: ATPR could suppress the migration and invasion of A549 cells, and the mechanism might be concerned with down-regulating the expression of MLCK in the ERK-MAPK signaling pathway, pointing to therapeutic prospects in lung cancer.

Ratiometric and discriminative visualization of autophagic processes with a novel dual-responded lysosome-specific fluorescent probe

Fan Zheng,Yeshuo Ma,Jipeng Ding,Shuai Huang,Shengwang Zhang,Xueyan Huang,Bin Feng,Hongliang Zeng,Fei Chen,Wenbin Zeng 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Background Autophagy is a critical self-eating pathway involved in numerous physiological and pathological processes. Lysosomal degradation of dysfunctional organelles and invading microorganisms is central to the autophagy mechanism and essential for combating disease-related conditions. Therefore, monitoring fluctuations in the lysosomal microenvironment is vital for tracking the dynamic process of autophagy. Although much effort has been put into designing probes for measuring lysosomal viscosity or pH separately, there is a need to validate the concurrent imaging of the two elements to enhance the understanding of the dynamic progression of autophagy. Methods Probe HFI was synthesized in three steps and was developed to visualize changes in viscosity and pH within lysosomes for real-time autophagy tracking. Then, the spectrometric determination was carried out. Next, the probe was applied to image autophagy in cells under nutrient-deprivation or external stress. Additionally, the performance of HFI to monitor autophagy was employed to evaluate acetaminophen-induced liver injury. Results We constructed a ratiometric dual-responsive probe, HFI, with a large Stokes shift over 200 nm, dualwavelength emission, and small background interference. The ratiometric fluorescent signal (R = I610/I460) of HFI had an excellent correlation with both viscosity and pH. More importantly, high viscosity and low pH had a synergistic promotion effect on the emission intensity of HFI, which enabled it to specially lit lysosomes without disturbing the inherent microenvironment. We then successfully used HFI to monitor intracellular autophagy induced by starvation or drugs in real-time. Interestingly, HFI also enabled us to visualize the occurrence of autophagy in the liver tissue of a DILI model, as well as the reversible effect of hepatoprotective drugs on this event. Conclusions In this study, we developed the first ratiometric dual-responsive fluorescent probe, HFI, for real-time revealing autophagic details. It could image lysosomes with minimal perturbation to their inherent pH, allowing us to track changes in lysosomal viscosity and pH in living cells. Ultimately, HFI has great potential to serve as a useful indicator for autophagic changes in viscosity and pH in complex biological samples and can also be used to assess drug safety.

Characterization and profiling of MicroRNAs in posterior silk gland of the silkworm (Bombyx mori)

Fei Song,Xin Wang,Chen Chen,Yangyang Fan,Shunming Tang,Jinshan Huang,Xijie Guo,Xingjia Shen 한국유전학회 2015 Genes & Genomics Vol.37 No.8

MicroRNAs (miRNAs) regulate expression of genes at post-transcriptional level by binding on complementary sequences of target mRNAs and play multiple roles in biological processes. To investigate the differential expression of miRNAs in posterior silk gland (PSG) of silkworm (Bombyx mori) in different periods and regulation of miRNAs on the expression of fibroin genes, Solexa sequencing technology was used to detect miRNAs in PSGs of fourth-instar day-2 larvae and fifth-instar day-3 larvae, respectively. As a result, 466 previously reported miRNAs, and 35 novel miRNAs were detected, and 499 of these detected miRNAs are predicted to target 13,383 genes by target prediction softwares. Additionally, 29 miRNAs expressed differently between the PSG of fourthinstar day-2 larvae and fifth-instar day-3 larvae were found, and the differential expression of these miRNAs may play an important role in the expression of fibroin genes.

A Hierarchical Single-Surface Model for an Unsaturated Soil-Structure Interface

Fei Peng,Xin Li,Meng-fan Lv,Yong-Hui Li,Yuan-Cheng Guo 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.6

In this paper, according to the shear test of the interface between unsaturated soil and steel plate, the modified apparent roughness is used to describe interface roughness. And considering the influence of matrix suction and interface roughness, the stress variable is constructed to improve the yield and plastic potential functions. Then a modified hierarchical single-surface elastoplastic interface constitutive model (HISS-Unsaturated-Int model) is set up. The finite element calculation of HISS-Unsaturated-Int model is achieved by compiling and calling the user subroutine FRIC of ABAQUS. The interface shear test between unsaturated soil and structure with different water contents and the pull-out shear test between unsaturated soil and soil nail are simulated. The calculation results are consistent with those of experiments, which shows the modified interface constitutive model can effectively predict the shear mechanical properties of soil-structure interface under different soil moisture content, normal stress and interface roughness.

Robust and Fast Tracking via Joint Collaborative Representation

Fei Zhou,Guizong Zhang,Xinyue Fan,Dandan Yi 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.4

In this paper, we present a robust and fast tracking method based on joint collaborative representation. Traditional sparse coding based tracking methods code the candidates as a sparse linear combination of a series of object and trivial templates and perform time consuming L1 regularizations. In contrast to these methods, this paper adopts the L2-regularized least square models to reduce the computational complexity. The tracked object can be represented by the linear combination of a series of object templates, and also can be represented by candidate samples in the current frame. We propose a joint objective function to handle the tracking process. In addition, we introduce an effective update scheme to deal with the change of target appearance over time. Experiments on several challenging image sequences show that our proposed tracking method is robust and efficient.