Regulation of the Edwardsiella tarda Hemolysin Gene and LuxS by EthR

Fang Wang,Min Zhang,Yong Hua Hu,Wei Wei Zhang,Li Sun 한국미생물 · 생명공학회 2009 Journal of microbiology and biotechnology Vol.19 No.8

The Beneficial Effects of a Polysaccharide from Moringa oleifera Leaf on Gut Microecology in Mice

Fang Wang,Yi-Fan Bao,Jia-Jun Si,Yu Duan,Ze-Bin Weng,Xin-Chun Shen 한국식품영양과학회 2019 Journal of medicinal food Vol.22 No.9

Moringa oleifera is a natural plant with high nutritional and pharmacological value. Leaves of M. oleifera contain a variety of active substances. In our previous research, we had obtained a polysaccharide separated from M. oleifera leaf, namely MOs-2-a (1.35 × 104 Da). In this study, this polysaccharide was administrated daily to 6 week-old ICR mice for 4 weeks. Then, the body weight, immunity, intestinal digestion, and intestinal microenvironment of Institute of Cancer Research (ICR) mice were investigated. After 4 weeks of feeding intervention with the polysaccharide, the immune and intestinal digestive ability of the ICR mice were significant as shown by the organ index, digestive enzymes, and reduction of serum tumor necrosis factor-alpha and diamine oxidase levels. The polysaccharide could regulate the microbial composition of the intestinal tract in mice by increasing the bacteria that have been reported for antiobesity effects, short chain fatty acid production, and lactic acid production. These findings indicate that the polysaccharide of M. oleifera leaf might be a promising prebiotic that exhibits health promotion effects.

Outcome-based Contractors Selection using the Integrated Fuzzy AHP and Fuzzy TOPISIS Method

Fang Wang,Quan Shi,Zhi-wei Hu,Wei Xia 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.4

Based on the concept of outcome-based maintenance, the outcome-based contractor equipment support is proposed as the logistical support strategy in the military industry. The outcome-based contractor equipment support is focus on the combat efficiency rather than on the performance of the equipments. Choosing the right contractor is a very important part in the process of its implementation. In fact, the contractor selection is a multi-criteria decision-making problem, which is largely dependent on the uncertainty and ambiguity of the decision-makers. This paper presents a systematic decision-making process of the outcome-based contractor selection, which employs fuzzy AHP to determine the importance weights of each index and adopts fuzzy TOPSIS to obtain the performance ratings of the alternative contractors. Finally, the validity of this method is verified by a specific case.

Effect of transformational leadership on organizational performance : Focusing on the mediating effect of decision participation by members of IT industry in China

Fang wang,Jun-ho Jang ASCONS 2022 IJASC Vol.4 No.2

Background/Objectives: This research emphasized the importance of Transformational leadership elements such as role model demonstration, vision motivation, individual consideration and charisma. Moreover, it focused on verifying the effects of these four elements on organizational performance through decision participation. In addition, the mediating role of decision participation was clearly identified as a key variable that can lead to organizational performance. Methods/Statistical analysis: To verify this, an empirical analysis was conducted on workers engaged in IT industries in China. Findings: Through empirical analysis, it was found that all four elements of Transformational leadership improve decision participation. Furthermore, decision participation was found to increase the level of organizational performance. Thus, it was found that decision participation played a partial mediating role in the relationship between the four elements of Transformational leadership and organizational performance. Applications: It has great implications for global companies that have entered China or have plans to enter the country in the future by sampling members of the IT industry in China.

Effect of IgY on Periodontitis and Halitosis Induced by Fusobacterium nucleatum

( Fang Wang ),( Wu Qiao ),( Bin Bao ),( Shujun Wang ),( Joe Mac Regenstein ),( Yilei Shi ),( Wenhui Wu ),( Ming Ma ) 한국미생물 · 생명공학회 2019 Journal of microbiology and biotechnology Vol.29 No.2

Fusobacterium nucleatum is a morbific agent in periodontitis and halitosis. Egg yolk antibody (IgY) was obtained from egg yolks from chickens stimulated with F. nucleatum. This study was to assess the effectiveness of IgY on periodontitis and halitosis caused by F. nucleatum in vitro and in vivo. The growth of F. nucleatum was inhibited (p <0. 05) by different concentrations of IgY in vitro and the results of a Halimeter show volatile sulfur compounds (VSCs) were reduced to 904 ± 57 ppb at a concentration 40 mg/ml of IgY. The changes of fatty acids of F. nucleatum were determined using GC-MS. The scores for odor index of rat saliva were decreased. The major constituent of volatile organic compounds (VOCs) including short-chain acids decreased 46.2% in 10 mg/ml IgY, ammonia decreased 70% in 40 mg/ml IgY, while aldehydes and olefine ketones were almost unchanged. The ELISA assay revealed that IL-6 and TNF-α were decreased after 4 weeks’ IgY treatment. Morphometric (X-ray) and histological analyses (HE) showed that IgY reduced alveolar bone loss and collagen fibers became orderly in rat models. As a result, IgY may have the potential to treat periodontitis and halitosis.

Disturbance Observer-based Nonsingular Fast Terminal Sliding Mode Fault Tolerant Control of a Quadrotor UAV with External Disturbances and Actuator Faults

Fang Wang,Zhigang Ma,Hongmei Gao,Chao Zhou,Changchun Hua 제어·로봇·시스템학회 2022 International Journal of Control, Automation, and Vol.20 No.4

This paper addresses a nonsingular fast terminal sliding mode fault tolerant control (NFTSMFTC) scheme based on disturbance observer (DO) for a quadrotor UAV in the presence of external disturbances and actuator additive faults. Firstly, by analyzing the dynamic characteristics of a quadrotor UAV, the model is decoupled into outer-loop (position subsystem) and inner-loop (attitude subsystem). Secondly, the DO is utilized to deal with external disturbances and actuator additive faults effectively. Furthermore, nonsingular fast terminal sliding mode control (NFTSMC) can avoid the chattering problems of traditional sliding mode control (SMC) and singularity problems of terminal SMC. Moreover, compared with nonsingular terminal SMC, it behaves the faster convergence rate. So the NFTSMC method is applied to design controller. Finally, in the framework of Lyapunov theory, the stability of the closed-loop system is analyzed, and the tracking error is asymptotically stable. The effectiveness of the proposed control scheme is verified by simulation results.

Design of online damage images detection system for large-aperture mirrors of high power laser facility based on wavefront coding technology

Fang, Wang,Qinxiao, Liu,Dongxia, Hu,Hongjie, Liu,Tianran, Zheng Korean Nuclear Society 2021 Nuclear Engineering and Technology Vol.53 No.9

The laser transport system of the high power laser facility is mainly composed of large-aperture laser transport mirrors (TMs). Obtaining the high-resolution online damage images during the operation, which is of great significance for operating safely of the mirrors and the facility. Based on wavefront coding, pan-tilt scanning and image stitching technologies, an online laser-damage images detection system is designed, and it can achieve high-precision detection of surface characteristics of large-aperture laser transport mirrors. The preliminary simulation proves that the system can solve the depth of field matching problem caused by pan-tilt tilt imaging and achieve higher resolution.

Robust Adaptive Backstepping Control for an Uncertain Nonlinear System with Input Constraint based on Lyapunov Redesign

Fang Wang,Qin Zou,Qun Zong 제어·로봇·시스템학회 2017 International Journal of Control, Automation, and Vol.15 No.1

This paper presents the control problem for a class of n-order semi-strict nonlinear system subjects tounknown parameters, uncertainty, and input constraint. The controller is designed via combining backsteppingcontrol and Lyapunov redesign. Firstly, based on the Lyapunov function, a composite adaptive law is constructedto estimate the unknown parameters. To sequel, a robust term is designed to handle the matched and unmatcheduncertainties on the basis of Lyapunov redesign technique. The “explosion of terms” problem that inherent inbackstepping control is avoided by the robust second-order filters. Thirdly, an auxiliary signal provided by theauxiliary system is employed to handle the influence of the input constraint. It is proved that the closed-loopsystem is stable in a Lyapunov framework theory and the semi-global uniformly ultimate boundedness of all signalsis achieved. Finally, numerical simulations are carried out to evaluate the performance of the proposed controlstrategy. Numerical example and the application of the hypersonic vehicle (HSV) tracking control are simulated todemonstrate the effectiveness of the proposed control scheme.

miR-638 is a new biomarker for outcome prediction of non-small cell lung cancer patients receiving chemotherapy

Fang Wang,Jian-fang Lou,Yan Cao,Xin-hui Shi,Peng Wang,Jian Xu,Er-fu Xie,Ting Xu,Rui-hong Sun,Jianyu Rao,Pu-wen Huang,Shi-yang Pan,Hong Wang 생화학분자생물학회 2015 Experimental and molecular medicine Vol.47 No.-

MicroRNAs (miRNAs), a class of small non-coding RNAs, mediate gene expression by either cleaving target mRNAs or inhibiting their translation. They have key roles in the tumorigenesis of several cancers, including non-small cell lung cancer (NSCLC). The aim of this study was to investigate the clinical significance of miR-638 in the evaluation of NSCLC patient prognosis in response to chemotherapy. First, we detected miR-638 expression levels in vitro in the culture supernatants of the NSCLC cell line SPC-A1 treated with cisplatin, as well as the apoptosis rates of SPC-A1. Second, serum miR-638 expression levels were detected in vivo by using nude mice xenograft models bearing SPC-A1 with and without cisplatin treatment. In the clinic, the serum miR-638 levels of 200 cases of NSCLC patients before and after chemotherapy were determined by quantitative real-time PCR, and the associations of clinicopathological features with miR-638 expression patterns after chemotherapy were analyzed. Our data helped in demonstrating that cisplatin induced apoptosis of the SPC-A1 cells in a dose- and time-dependent manner accompanied by increased miR-638 expression levels in the culture supernatants. In vivo data further revealed that cisplatin induced miR-638 upregulation in the serum derived from mice xenograft models, and in NSCLC patient sera, miR-638 expression patterns after chemotherapy significantly correlated with lymph node metastasis. Moreover, survival analyses revealed that patients who had increased miR-638 levels after chemotherapy showed significantly longer survival time than those who had decreased miR-638 levels. Our findings suggest that serum miR-638 levels are associated with the survival of NSCLC patients and may be considered a potential independent predictor for NSCLC prognosis.

BmNPV chitinase gene deletion enhances foreign gene expression in a BmN cell system

Fang WANG,Chuan-Xi ZHANG 한국곤충학회 2009 Entomological Research Vol.39 No.1

Bombyx mori nucleopolyhedrovirus (BmNPV) is extensively being studied as an expression vector for heterologous gene expression in silkworm-derived cells as well as in the host larvae or pupae. BmNPV chitinase is necessary for liquefaction of the virus-infected host insect. The influence of chitinase on the efficiency of foreign gene expression was studied to provide a scientific basis for improving the BmNPV expression system. The BmNPV chitinase gene (chiA) was deleted and the expression level of the polyhedrin promoter controlling the lacZ gene in BmN cells was determined. Sodium dodecylsulfate (SDS)–olyacrylamide gel electrophoresis (PAGE) showed that β-galactosidase accounted for approximately 6.9 and 7.7% of the total protein in BmN cells infected with the chiA deficient Bm lacZ+chiA−,at 3 and 4 days post infection, while the total protein was 3.2 and 4.2% in cells infected with Bm lacZ+. The relative β-galactosidase activities in Bm lacZ+chiA−[기호] -infected cells improved 2.33- and 1.56-fold compared to those of Bm lacZ+-infected cells at 3 and 4 days post infection. The results of the present study suggest that chitinase deletion could improve the lacZ expression level in the BmNPV-BmN cell expression system.