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Zhu, M.J.,Yu, M.,Liu, B.,Zhu, Z.Z.,Xiong, T.A.,Fan, B.,Xu, S.P.,Du, Y.Q.,Peng, Z.Z.,Li, K. Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.5
The Tongcheng pig breed is a famous Chinese indigenous breed. The Ministry of Agriculture of China has filed it as 1 of 19 national key conservation breeds selected from more than 100 Chinese indigenous pig breeds in 2000. In order to improve the reproductive performance, it has been intensively selected to increase the litter size for about 10 years. The population randomly sampled from conservation nucleus of eight families in the Tongcheng pigs was genotyped for identification of their estrogen receptor locus polymorphisms with the PCR-RFLPs method. Only AB heterozygotes and BB homozygotes were detected, and $X^2$ test demonstrated that the locus was in disequilibrium at a significant level (p<0.05). In the present paper, the litter sizes in different parities were regarded as different traits. Holistic status of other unspecific and unidentified genes was estimated by using the statistical methods. Coefficients of kurtosis and skewness showed that the litter size still presented segregating characteristic in the 2nd, 5th, 7th, 8th and 9th parities. Analysis of homogeneity of variance between families confirmed the results for the 5th, 7th and 8th parities. The heritability of litter size for the 1st to 10th parities was estimated with paternal half-sib model and individual estimated breeding values (EBVs) were evaluated by a single trait animal model as well. We found that the averages of EBVs for litter size in each parity did not differ significantly between genotypes, despite the significant difference for original phenotype records in the 3rd, 4th and 5th parities (p<0.05 or p<0.01). The results may be explained by the deduction that the polymorphisms of ESR locus are no longer the important genetic base of litter size variation when the frequency of allele B accumulated in the experience of selection procedure, and further conferring that there exist special genes associated with litter size in the recent Tongcheng pigs population can be made.
Zhu, Qiankun,Zhu, Mengli,Fan, Gaotao,Zou, Jiaxin,Feng, Peichun,Liu, Zubi,Wang, Wanjun Korean Chemical Society 2014 Bulletin of the Korean Chemical Society Vol.35 No.1
Coptis chinensis 3'-hydroxy-N-methylcoclaurine 4'-O-methyltransferase (HOMT), an essential enzyme in the berberine biosynthetic pathway, catalyzes the methylation of 3'-hydroxy-N-methylcoclaurine (HMC) producing reticuline. A 3D model of HOMT was constructed by homology modeling and further subjected to docking with its ligands and molecular dynamics simulations. The 3D structure of HOMT revealed unique structural features which permitted the methylation of HMC. The methylation of HMC was proposed to proceed by deprotonation of the 4'-hydroxyl group via His257 and Asp258 of HOMT, followed by a nucleophilic attack on the SAM-methyl group resulting in reticuline. HOMT showed high substrate specificity for methylation of HMC. The study evidenced that Gly117, Thr312 and Asp258 in HOMT might be the key residues for orienting substrate for specific catalysis.
Zhu, Fan,Li, Jian,Li, Wen-Xin,Liu, Zhong-Chun,Long, Xing Korean Society for Biochemistry and Molecular Biol 2004 Journal of biochemistry and molecular biology Vol.37 No.6
The expression and clinicopathological significance of Quox-1 gene was studied in oral squamous cell carcinoma (OSCC). Immunocytochemistry and western blot analysis were used to examine the different expressions of Quox-1 protein in 114 OSCC specimens, 34 oral epithelial dysplasia specimens, and 16 normal oral mucosa specimens. RT-PCR and virtual Northern Blot were also used to examine the expression of Quox-1 mRNA. It was found that Quox-1 was not expressed in normal epithelium. However, as dysplastic lesions progressed Quox-1 expression increased (p < 0.01), and Quox-1 expression was not significantly different between severe dysplasia and highly differentiated OSCCs (p > 0.05). As the degree of differentiation decreased, Quox-1 positivity increased in OSCC (p < 0.01), and the rate of Quox-1 (81.58%) positivity in OSCC was higher than that in normal oral mucosa (p < 0.01). Our findings imply that the positive expression of Quox-1 is correlated with the histological classification of OSCCs. Thus, the expression of Quox-1 in OSCC may serve as a significant predicting factor of proliferative status and malignant degree, and it may also be a biological detection marker of oral mucosas initial cancer and of OSCC.
Fabrication of recyclable UiO-66-NH2/PVDF hybrid fibrous membrane for Cr(VI) removal in wastewater
Fan Yu,Liusha Cen,Caihong Lei,Feichao Zhu,Lan Zhou,Hailin Zhu,Bin Yu 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.123 No.-
Efficient and recyclable water treatment technology plays a vital role in practical application of Cr(Ⅵ)removal. Zr-based metal–organic framework (UiO-66-NH2) is widely used in wastewater treatmentdue to its water stability. Powdery UiO-66-NH2 has disadvantages of easy aggregation, reclamation difficultyand secondary pollution. In this work, UiO-66-NH2 was successfully loaded on electrospunpolyvinylidene fluoride (PVDF) fibers by in-situ growth to achieve efficient adsorption and photoreductionof Cr(Ⅵ). Grooved surface of PVDF fibers and truncated octahedron shape of UiO-66-NH2 ensuredthe loading uniformity and firmness. The loading rate and size of UiO-66-NH2 crystals increased withthe amount of trifluoroacetic acid (TFA) in the preparation process. Meantime, the introduction of TFAin synthesis was conducive to the separation efficiency of photogenerated carries. When the additionamount of TFA was 5 ml, UiO-PVDF-3 with loading rate of 44.8% exhibited best Cr(VI) adsorption andphotocatalysis performance. For the pure adsorption process, the adsorption capacity of UiO-PVDF-3 at90 min was 3.76 mg/cm2, and the corresponding removal rate was 95.8%. When visible light was applied,the Cr(VI) removal rate for UiO-PVDF-3 reached to about 95.0% at 45 min. Furthermore, regenerationexperiments showed UiO-PVDF-3 had excellent adsorption and photocatalysis reusability. Based on thismethod, synthesized UiO-66-NH2/PVDF hybrid membrane as a bifunctional material for adsorption andphotocatalysis realizes the reuse of UiO-66-NH2 crystals conveniently and avoids secondary pollutionduring Cr(VI) wastewater treatment.
( Fan Zhu ),( Jian Li ),( Wen Xin Li ),( Zhong Chun Liu ),( Xing Long ) 생화학분자생물학회 2004 BMB Reports Vol.37 No.6
The expression and clinicopathological significance of Quox-1 gene was studied in oral squamous cell carcinoma (OSCC). Immunocytochemistry and western blot analysis were used to examine the different expressions of Quox-1 protein in 114 OSCC specimens, 34 oral epithelial dysplasia specimens, and 16 normal oral mucosa specimens. RT-PCR and virtual Northern Blot were also used to examine the expression of Quox-1 mRNA. It was found that Quox-1 was not expressed in normal epithelium. However, as dysplastic lesions progressed Quox-1 expression increased (p < 0.01), and Quox-1 expression was not significantly different between severe dysplasia and highly differentiated OSCCs (p > 0.05). As the degree of differentiation decreased, Quox-1 positivity increased in OSCC (p < 0.01), and the rate of Quox-1 (81.58%) positivity in OSCC was higher than that in normal oral mucosa (p < 0.01). Our findings imply that the positive expression of Quox-1 is correlated with the histological classification of OSCCs. Thus, the expression of Quox-1 in OSCC may serve as a significant predicting factor of proliferative status and malignant degree, and it may also be a biological detection marker of oral mucosas initial cancer and of OSCC.
CONTINUOUS DEPENDENCE PROPERTIES ON SOLUTIONS OF BACKWARD STOCHASTIC DIFFERENTIAL EQUATION
Fan, Sheng-Jun,Wu, Zhu-Wu,Zhu, Kai-Yong 한국전산응용수학회 2007 Journal of applied mathematics & informatics Vol.24 No.1
The existence theorem and continuous dependence property in $"L^2"$ sense for solutions of backward stochastic differential equation (shortly BSDE) with Lipschitz coefficients were respectively established by Pardoux-Peng and Peng in [1,2], Mao and Cao generalized the Pardoux-Peng's existence and uniqueness theorem to BSDE with non-Lipschitz coefficients in [3,4]. The present paper generalizes the Peng's continuous dependence property in $"L^2"$ sense to BSDE with Mao and Cao's conditions. Furthermore, this paper investigates the continuous dependence property in "almost surely" sense for BSDE with Mao and Cao's conditions, based on the comparison with the classical mathematical expectation.