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Impact of a Glyphosate-Tolerant Soybean Line on the Rhizobacteria, Revealed by Illumina MiSeq
( Gui-hua Lu ),( Yin-ling Zhu ),( Ling-ru Kong ),( Jing Cheng ),( Cheng-yi Tang ),( Xiao-mei Hua ),( Fan-fan Meng ),( Yan-jun Pang ),( Rong-wu Yang ),( Jin-liang Qi ),( Yong-hua Yang ) 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.3
The global commercial cultivation of transgenic crops, including glyphosate-tolerant soybean, has increased widely in recent decades with potential impact on the environment. The bulk of previous studies showed different results on the effects of the release of transgenic plants on the soil microbial community, especially rhizosphere bacteria. In this study, comparative analyses of the bacterial communities in the rhizosphere soils and surrounding soils were performed between the glyphosate-tolerant soybean line NZL06-698 (or simply N698), containing a glyphosate-insensitive EPSPS gene, and its control cultivar Mengdou12 (or simply MD12), by a 16S ribosomal RNA gene (16S rDNA) amplicon sequencing-based Illumina MiSeq platform. No statistically significant difference was found in the overall alpha diversity of the rhizosphere bacterial communities, although the species richness and evenness of the bacteria increased in the rhizosphere of N698 compared with that of MD12. Some influence on phylogenetic diversity of the rhizosphere bacterial communities was found between N698 and MD12 by beta diversity analysis based on weighted UniFrac distance. Furthermore, the relative abundances of part rhizosphere bacterial phyla and genera, which included some nitrogen-fixing bacteria, were significantly different between N698 and MD12. Our present results indicate some impact of the glyphosate-tolerant soybean line N698 on the phylogenetic diversity of rhizosphere bacterial communities together with a significant difference in the relative abundances of part rhizosphere bacteria at different classification levels as compared with its control cultivar MD12, when a comparative analysis of surrounding soils between N698 and MD12 was used as a systematic contrast study.
Fan, Ting-Ting,Cheng, Ying,Wang, Yin-Feng,Gui, Shu-Yu,Chen, Fei-Hu,Zhou, Qing,Wang, Yuan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.18
Aim: To observe the effects of a novel all-trans retinoid acid (ATRA) derivative, N-(3-trifluoromethyl-phenyl)-retinamide (ATPR), on lung adenocarcinoma A549 cells and to explore the potential mechanism of ATPR inhibiting of A549 cell migration. Materials and Methods: The cytotoxicity of ATRA and ATPR on A549 cells was assessed using MTT assay. Wound healing assays were used to analyze the influences of ATRA, ATPR, ML-7 (a highly selective inhibitor of myosin light chain kinase (MLCK)), PMA (an activator of MAPKs) and PD98059 (a selective inhibitor of ERK1/2) on the migration of A549 cells. Expression of MLCK and phosphorylation of myosin light chain (MLC) were assessed by Western blotting. Results: ATRA and ATPR inhibited the proliferation of A549 cells in a dose- and time-dependent manner, and the effect of ATPR was much more remarkable compared with ATRA. Relative migration rate and migration distance of A549 cells both decreased significantly after treatment with ATPR or ML-7. The effect on cell migration of PD98059 combining ATPR treatment was more notable than that of ATPR alone. Moreover, compared with control groups, the expression levels of MLCK and phosphorylated MLC in A549 cells were both clearly reduced in ATRA and ATPR groups. Conclusions: ATPR could suppress the migration and invasion of A549 cells, and the mechanism might be concerned with down-regulating the expression of MLCK in the ERK-MAPK signaling pathway, pointing to therapeutic prospects in lung cancer.
Failure Pattern of Singe-layer Reticulated Dome under Severe Earthquake and Its Shaking Table Test
Gui-Bo Nie,Xudong Zhi,Feng Fan,Jun-Wu Dai 한국강구조학회 2014 International Journal of Steel Structures Vol.14 No.2
Based on the constitutive model with considering material damage accumulation, the influence of material damageaccumulation on the responses of the structure is investigated in detail through the incremental dynamic analysis method (IDAanalysis). The failure pattern of single-layer reticulated dome under dynamic loading is obtained using IDA analysis throughmultiple sets of seismic load records and harmonic loading, which is identified to be the dynamic instability failure caused bythe geometric nonlinearity and the dynamic strength failure resulted by the material nonlinearity. The shaking table tests ofsingle-layer reticulated dome are exploited to verify the failure pattern of the structure under seismic load records. Experimentalresults show that the plasticity of the model develops and the deformation increases, as well as the mechanical propertiesweakens continuously with the increasing of the loading value, which indicates that the failure pattern of the structure isdynamic strength failure.
Expressions of meromorphic solutions of a certain type of nonlinear complex differential equations
Jun-Fan Chen,Gui Lian 대한수학회 2020 대한수학회보 Vol.57 No.4
In this paper, the expressions of meromorphic solutions of the following nonlinear complex differential equation of the form $$f^{n}+Q_{d}(z,f)=\sum_{i=1}^{3}p_{i}(z)e^{\alpha_{i}(z)}$$ are studied by using Nevanlinna theory, where $n\geq5$ is an integer, $Q_{d}(z,f)$ is a differential polynomial in $f$ of degree $d\leq n-4$~with rational functions as its coefficients, $p_{1}(z)$, $p_{2}(z)$, $p_{3}(z)$~are non-vanishing rational functions, and $\alpha_{1}(z)$, $\alpha_{2}(z)$, $\alpha_{3}(z)$ are nonconstant polynomials such that $\alpha_{1}'(z)$, $\alpha_{2}'(z)$, $\alpha_{3}'(z)$ are distinct each other. Moreover, examples are given to illustrate the accuracy of the condition.
( Wen Guang Fan ),( Gui Cheng Huo ),( Xiao Min Li ),( Li Jie Yang ),( Cui Cui Duan ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.2
The development of the gut is controlled and modulated by different interacting mechanisms, such as genetic endowment, intrinsic biological regulatory functions, environment influences and last but no least, the diet influence. In this work, we compared the fecal microbiota of breast-fed (BF), formula-fed (FF), and mixed-fed (MF) infants from Hebei Province, China. By using high-throughput 16S rDNA sequencing analyses, we found some differences in gut microbiota in the three groups. Firmicutes and Proteobacteria were the dominant bacteria at the phylum level in the three groups, where FF infants showed a significant depletion in Bacteroidetes (p < 0.001) and Actinobacteria (p < 0.05). Enterobacteriaceae was the dominant bacteria at the family level in the three groups, but FF infants showed higher Enterobacteriaceae enrichment than BF and MF infants (p < 0.05); the abundance of the Bifidobacteriaceae was only 8.16% in the feces of BF infants, but higher than in MF and FF infants (p < 0.05). The number of genera detected (abundance >0.01%) in BF, MF, and FF infants was only 15, 16, and 13, respectively. This study could provide more accurate and scientific data for the future study of infant intestinal flora.
EXPRESSIONS OF MEROMORPHIC SOLUTIONS OF A CERTAIN TYPE OF NONLINEAR COMPLEX DIFFERENTIAL EQUATIONS
Chen, Jun-Fan,Lian, Gui Korean Mathematical Society 2020 대한수학회보 Vol.57 No.4
In this paper, the expressions of meromorphic solutions of the following nonlinear complex differential equation of the form $$f^n+Qd(z,f)=\sum\limits_{i=1}^{3}pi(z)e^{{\alpha}_i(z)}$$ are studied by using Nevanlinna theory, where n ≥ 5 is an integer, Q<sub>d</sub>(z, f) is a differential polynomial in f of degree d ≤ n - 4 with rational functions as its coefficients, p<sub>1</sub>(z), p<sub>2</sub>(z), p<sub>3</sub>(z) are non-vanishing rational functions, and α<sub>1</sub>(z), α<sub>2</sub>(z), α<sub>3</sub>(z) are nonconstant polynomials such that α'<sub>1</sub>(z), α'<sub>2</sub>(z), α'<sub>3</sub>(z) are distinct each other. Moreover, examples are given to illustrate the accuracy of the condition.
Research on the Judgement Method for Catenary Action of Restrained Steel Beams in Fire
Meijing Liu,Shenggang Fan,Yang Guo,Heyang Gui,Runmin Ding,Hang Zhou 한국강구조학회 2020 International Journal of Steel Structures Vol.20 No.2
Based on fire tests, a parametric numerical simulation on the catenary action of restrained steel beams in fire was performed by the finite element software ABAQUS. It can be known that axial constraint stiffness, rotational constraint stiffness and load ratio are key factors that affect the catenary action in fire. The formation mechanism of catenary action under ISO-834 standard fire was studied; and the judgement method for catenary action in fire was presented. The results show that: (1) when axial constraint stiffness reaches a certain value, the catenary action of restrained steel beams will occur almost at the same time; (2) with the increase of rotational constraint stiffness or load ratio, the catenary action of steel beams will occur earlier; (3) for restrained steel beams under a small load ratio, the catenary action usually occurs in fire when the mid-span deformation reaches 1/15 of the span; but for restrained steel beams under a large load ratio, the catenary action usually occurs when the mid-span deformation reaches 1/18 of the span; (4) for restrained steel beams with a large rotational constraint stiffness, the judgement method for catenary action in fire can still adopt the same method as above, which will be a little conservative.
Tucker Modeling based Kronecker Constrained Block Sparse Algorithm
( Tingping Zhang ),( Shangang Fan ),( Yunyi Li ),( Guan Gui ),( Yimu Ji ) 한국인터넷정보학회 2019 KSII Transactions on Internet and Information Syst Vol.13 No.2
This paper studies synthetic aperture radar (SAR) imaging problem which the scatterers are often distributed in block sparse pattern. To exploiting the sparse geometrical feature, a Kronecker constrained SAR imaging algorithm is proposed by combining the block sparse characteristics with the multiway sparse reconstruction framework with Tucker modeling. We validate the proposed algorithm via real data and it shows that the our algorithm can achieve better accuracy and convergence than the reference methods even in the demanding environment. Meanwhile, the complexity is smaller than that of the existing methods. The simulation experiments confirmed the effectiveness of the algorithm as well.
Han-Qin Shen,Zhuan-Qiang Yan,Fan-Gui Zeng,Chang-Tao Liao,Qing-Feng Zhou,Jian-Ping Qin,Qingmei Xie,Yingzuo Bi,Feng Chen 대한수의학회 2015 Journal of Veterinary Science Vol.16 No.3
As part of our ongoing influenza surveillance program in South China, 19 field strains of H9N2 subtype avian influenza viruses (AIVs) wereisolated from dead or diseased chicken flocks in Guangdong province, South China, between 2012 and 2013. Hemagglutinin (HA) genes ofthese strains were sequenced and analyzed and phylogenic analysis showed that 12 of the 19 isolates belonged to the lineage h9.4.2.5, whilethe other seven belonged to h9.4.2.6. Specifically, we found that all of the viruses isolated in 2013 belonged to lineage h9.4.2.5. The lineageh9.4.2.5 viruses contained a PSRSSR↓GLF motif at HA cleavage site, while the lineage h9.4.2.6 viruses contained a PARSSR↓GLF at thesame position. Most of the isolates in lineage h9.4.2.5 lost one potential glycosylation site at residues 200–202, and had an additional oneat residues 295–297 in HA1. Notably, 19 isolates had an amino acid exchange (Q226L) in the receptor binding site, which indicated that theviruses had potential affinity of binding to human like receptor. The present study shows the importance of continuing surveillance of newH9N2 strains to better prepare for the next epidemic or pandemic outbreak of H9N2 AIV infections in chicken flocks.