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Fusarium Species from Sorghum in Thailand
Mohamed Nor, Nik M.I.,Salleh, Baharuddin,Leslie, John F. The Korean Society of Plant Pathology 2019 Plant Pathology Journal Vol.35 No.4
Sorghum is the fifth most important cereal worldwide, spreading from Africa throughout the world. It is particularly important in the semi-arid tropics due to its drought tolerance, and when cultivated in Southeast Asia commonly occurs as a second crop during the dry season. We recovered Fusarium from sorghum in Thailand and found F. proliferatum, F. thapsinum and F. verticillioides most frequently, and intermittent isolates of F. sacchari and F. beomiforme. The relatively high frequencies of F. proliferatum and F. verticillioides, suggest mycotoxin contamination, particularly fumonisins and moniliformin, should be evaluated. Genetic variation within the three commonly recovered species was characterized with vegetative compatibility, mating type, Amplified Fragment Length Polymorphisms (AFLPs), and female fertility. Effective population number ($N_e$) was highest for F. verticillioides and lowest for F. thapsinum with values based on mating type allele frequencies higher than those based on female fertility. Based on AFLP genetic variation, the F. thapsinum populations were the most closely related, the F. verticillioides populations were the most distantly related, and the F. proliferatum populations were in an intermediate position. The genetic variation observed could result if F. thapsinum is introduced primarily with seed, while F. proliferatum and F. verticillioides could arrive with seed or be carried over from previous crops, e.g., rice or maize, which sorghum is following. Confirmation of species transmission patterns is needed to understand the agricultural systems in which sorghum is grown in Southeast Asia, which are quite different from the systems found in Africa, Australia, India and the Americas.
CANDELS Sheds Light on the Environmental Quenching of Low-mass Galaxies
Guo, Yicheng,Bell, Eric F.,Lu, Yu,Koo, David C.,Faber, S. M.,Koekemoer, Anton M.,Kurczynski, Peter,Lee, Seong-Kook,Papovich, Casey,Chen, Zhu,Dekel, Avishai,Ferguson, Henry C.,Fontana, Adriano,Giavalis American Astronomical Society 2017 ASTROPHYSICAL JOURNAL LETTERS - Vol.841 No.2
<P>We investigate the environmental quenching of galaxies, especially those with stellar masses (M-*) < 10(9.5) Me-circle dot, beyond the local universe. Essentially all local low-mass quenched galaxies (QGs) are believed to live close to massive central galaxies, which is a demonstration of environmental quenching. We use CANDELS data to test whether or not such a dwarf QG-massive central galaxy connection exists beyond the local universe. For this purpose, we only need a statistically representative, rather than complete, sample of low-mass galaxies, which enables our study to z greater than or similar to 1.5. For each low-mass galaxy, we measure the projected distance (d(proj)) to its nearest massive neighbor (M-* > 10(10.5) M-circle dot) within a redshift range. At a given z and M-*, the environmental quenching effect is considered to be observed if the d(proj) distribution of QGs (d(proj)(Q)) is significantly skewed toward lower values than that of star-forming galaxies (d(proj)(SF)). For galaxies with 10(8) M-circle dot < M-* < 10(10) M-circle dot, such a difference between d(proj)(Q) and d(proj)(SF) is detected up to z similar to 1. Also, about 10% of the quenched galaxies in our sample are located between two and four virial radii (R-Vir) of the massive halos. The median projected distance from low-mass QGs to their massive neighbors, d(proj)(Q)/R-Vir, decreases with satellite M-* at M-* less than or similar to 10(9.5) M-circle dot, but increases with satellite M-* at M-* greater than or similar to 10(9.5) M-circle dot. This trend suggests a smooth, if any, transition of the quenching timescale around M-* similar to 10(9.5) M-circle dot at 0.5 < z < 1.0.</P>
Estrada-Angulo, A.,Lopez-Soto, M.A.,Rivera-Mendez, C.R.,Castro, B.I.,Rios, F.G.,Davila-Ramos, H.,Barreras, A.,Urias-Estrada, J.D.,Zinn, R.A.,Plascencia, A. Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.12
Recent findings have shown that microbial nitrogen flow and digestible energy of diets are increased when urea is combined with a slow-release urea (SRU) in diets with a starch to acid detergent fibre ratio (S:F) 4:1. This affect is attributable to enhanced synchrony between ruminal N availability for microbial growth and carbohydrate degradation. To verify the magnitude of this effects on lamb performance, an experiment was conducted to evaluate the effects of combining urea and a SRU in diets containing S:F ratios of 3:1, 4:1, or 5:1 on performance, dietary energetics and carcass characteristics of finishing lambs. For that, 40 Pelibuey${\times}$Katahdin lambs ($36.65{\pm}3kg$) were assigned to one of five weight groupings in 20 pens (5 repetition/treatments). The S:F ratio in the diet was manipulated by partially replacing the corn grain and dried distiller's grain with solubles by forage (wheat straw) and soybean meal to reach S:F ratios of 3:1, 4:1 or 5:1. An additional treatment of 4:1 S:F ratio with 0.8% urea as the sole source of non-protein nitrogen was used as a reference for comparing the effect of urea combination vs. conventional urea at the same S:F ratio. There were no treatment effects on dry matter intake (DMI). Compared the urea combination vs urea at the same S:F ratio, urea combination increased (p<0.01) average daily gain (ADG, 18.3%), gain for feed (G:F, 9.5%), and apparent energy retention per unit DMI (8.2%). Irrespective of the S:F ratio, the urea combination improved the observed-to-expected dietary ratio and apparent retention per unit DMI was maximal (quadratic effect, $p{\leq}0.03$) at an S:F ratio of 4:1, while the conventional urea treatment did not modify the observed-to-expected net energy ratio nor the apparent retention per unit DMI at 4:1 S:F ratio. Urea combination group tended (3.8%, p = 0.08) to have heavier carcasses with no effects on the rest of carcass characteristics. As S:F ratio increased, ADG, G:F, dietary net energy, carcass weight, dressing percentage and longissimus thoracis (LM) area increased linearly ($p{\leq}0.02$). Combining urea and a slow-release urea product results in positive effects on growth performance and dietary energetics, but the best responses are apparently observed when there is a certain proportion (S:F ratio = 4:1) of starch to acid detergent fibre in the diet.
Abbott, B. P.,Abbott, R.,Abbott, T. D.,Abernathy, M. R.,Acernese, F.,Ackley, K.,Adams, C.,Adams, T.,Addesso, P.,Adhikari, R. X.,Adya, V. B.,Affeldt, C.,Agathos, M.,Agatsuma, K.,Aggarwal, N.,Aguiar, O. American Physical Society 2016 Physical Review D Vol.94 No.6
<P>We compare GW150914 directly to simulations of coalescing binary black holes in full general relativity, including several performed specifically to reproduce this event. Our calculations go beyond existing semianalytic models, because for all simulations-including sources with two independent, precessing spins - we perform comparisons which account for all the spin-weighted quadrupolar modes, and separately which account for all the quadrupolar and octopolar modes. Consistent with the posterior distributions reported by Abbott et al. [Phys. Rev. Lett. 116, 241102 (2016)] (at the 90% credible level), we find the data are compatible with a wide range of nonprecessing and precessing simulations. Follow-up simulations performed using previously estimated binary parameters most resemble the data, even when all quadrupolar and octopolar modes are included. Comparisons including only the quadrupolar modes constrain the total redshifted mass M-z epsilon [64 M-circle dot - 82 M-circle dot], mass ratio 1/q = m(2)/m(1) epsilon [0.6; 1], and effective aligned spin chi(eff) epsilon [-0.3, 0.2] where chi(eff) = (S-1/m(1)+S-2/m(2)). (L) over cap /M. Including both quadrupolar and octopolar modes, we find the mass ratio is even more tightly constrained. Even accounting for precession, simulations with extreme mass ratios and effective spins are highly inconsistent with the data, at any mass. Several nonprecessing and precessing simulations with similar mass ratio and chi(eff) are consistent with the data. Though correlated, the components' spins (both in magnitude and directions) are not significantly constrained by the data: the data is consistent with simulations with component spin magnitudes a(1,2) up to at least 0.8, with random orientations. Further detailed follow-up calculations are needed to determine if the data contain a weak imprint from transverse (precessing) spins. For nonprecessing binaries, interpolating between simulations, we reconstruct a posterior distribution consistent with previous results. The final black hole's redshifted mass is consistent with M-f,M-z in the range 64.0 M-circle dot - 73.5 M-circle dot and the final black hole's dimensionless spin parameter is consistent with a(f) = 0.62-0.73. As our approach invokes no intermediate approximations to general relativity and can strongly reject binaries whose radiation is inconsistent with the data, our analysis provides a valuable complement to Abbott et al.</P>
Lopez-Soto, M.A.,Rivera-Mendez, C.R.,Aguilar-Hernandez, J.A.,Barreras, A.,Calderon-Cortes, J.F.,Plascencia, A.,Davila-Ramos, H.,Estrada-Angulo, A.,Valdes-Garcia, Y.S. Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.2
As a result of the cost of grains, the replacement of grains by co-products (i.e. DDGS) in feedlot diets is a common practice. This change produces diets that contain a lower amount of starch and greater amount of fibre. Hypothetically, combining feed grade urea (U) with slow release urea (Optigen) in this type of diet should elicit a better synchrony between starch (high-rate of digestion) and fibre (low-rate of digestion) promoting a better microbial protein synthesis and ruminal digestion with increasing the digestible energy of the diet. Four cannulated Holstein steers ($213{\pm}4$ kg) were used in a $4{\times}4$ Latin square design to examine the combination of Optigen and U in a finishing diet containing different starch:acid detergent fibre ratios (S:F) on the characteristics of digestive function. Three S:F ratios (3.0, 4.5, and 6.0) were tested using a combination of U (0.80%) and Optigen (1.0%). Additionally, a treatment of 4.5 S:F ratio with urea (0.80% in ration) as the sole source of non-protein nitrogen was used to compare the effect of urea combination at same S:F ratio. The S:F ratio of the diet was manipulated by replacing the corn grain by dried distillers grain with solubles and roughage. Urea combination did not affect ruminal pH. The S:F ratio did not affect ruminal pH at 0 and 2 h post-feeding but, at 4 and 6 h, the ruminal pH decreased as the S:F ratio increased (linear, p<0.05). Ruminal digestion of OM, starch and feed N were not affected by urea combination or S:F ratio. The urea combination did not affect ADF ruminal digestion. ADF ruminal digestion decreased linearly (p = 0.02) as the S:F ratio increased. Compared to the urea treatment (p<0.05) and within the urea combination treatment (quadratic, p<0.01), the flow of microbial nitrogen (MN) to the small intestine and ruminal microbial efficiency were greater for the urea combination at a S:F ratio of 4.5. Irrespective of the S:F ratio, the urea combination improved (2.8%, p = 0.02) postruminal N digestion. As S:F ratio increased, OM digestion increased, but ADF total tract digestion decreased. The combination of urea at 4.5 S:F improved (2%, p = 0.04) the digestible energy (DE) more than expected. Combining urea and Optigen resulted in positive effects on the MN flow and DE of the diet, but apparently these advantages are observed only when there is a certain proportion of starch:ADF in the diet.
Fusarium Species from Sorghum in Thailand
Nik M. I. Mohamed Nor,Baharuddin Salleh,John F. Leslie 한국식물병리학회 2019 Plant Pathology Journal Vol.35 No.4
Sorghum is the fifth most important cereal worldwide, spreading from Africa throughout the world. It is particularly important in the semi-arid tropics due to its drought tolerance, and when cultivated in Southeast Asia commonly occurs as a second crop during the dry season. We recovered Fusarium from sorghum in Thailand and found F. proliferatum, F. thapsinum and F. verticillioides most frequently, and intermittent isolates of F. sacchari and F. beomiforme. The relatively high frequencies of F. proliferatum and F. verticillioides, suggest mycotoxin contamination, particularly fumonisins and moniliformin, should be evaluated. Genetic variation within the three commonly recovered species was characterized with vegetative compatibility, mating type, Amplified Fragment Length Polymorphisms (AFLPs), and female fertility. Effective population number (Ne) was highest for F. verticillioides and lowest for F. thapsinum with values based on mating type allele frequencies higher than those based on female fertility. Based on AFLP genetic variation, the F. thapsinum populations were the most closely related, the F. verticillioides populations were the most distantly related, and the F. proliferatum populations were in an intermediate position. The genetic variation observed could result if F. thapsinum is introduced primarily with seed, while F. proliferatum and F. verticillioides could arrive with seed or be carried over from previous crops, e.g., rice or maize, which sorghum is following. Confirmation of species transmission patterns is needed to understand the agricultural systems in which sorghum is grown in Southeast Asia, which are quite different from the systems found in Africa, Australia, India and the Americas.
Spricigo, J.F.W.,Morais, K.S.,Yang, B.S.,Dode, M.A.N. Academic Press 2012 Cryobiology Vol.65 No.3
The present study aimed to evaluate the effect of methyl-β-cyclodextrin (MβCD) as a cholesterol loader to change oocyte plasma membrane and increase its tolerance toward cryopreservation. The first and second experiments were conducted to investigate if MβCD could improve nuclear and cytoplasmic maturation after oocyte exposure to cold stress for 10 or 30min, respectively. No differences (P>0.05) in either experiment in the metaphase II (MII) rate of oocytes exposed to MβCD and cold stress; but these oocytes presented lower maturation rates than control groups. In the second experiment, a lower percentage of oocytes showed degenerated chromatin (P<0.05) after exposure to 2mg/mL of MβCD compared to the group exposed to 0mg/mL. However, no differences among treatments were observed in cytoplasmic maturation. Groups exposed to cold stress demonstrated a lower (P<0.05) capacity for embryonic development compared to the control groups. In the third experiment immature oocytes were exposed to MβCD and then, vitrified (cryotop). After warming, we observed that the ability to reach MII and chromatin degeneration were altered (P<0.05) by MβCD. The blastocysts rate (P<0.05) on D7 was higher in the 2mg/mLMβCD group, but an identical finding was not observed on D8 (P>0.05). Chromatin degeneration was higher in the vitrification groups. We conclude that MβCD improved nuclear maturation by reducing oocyte degeneration after cold stress or vitrification; however, more studies are required to clarify the usefulness of MβCD use in oocyte cryopreservation.
H.C. Liu,J.F. Huang,S.R. Lee,H.L. Liu,C.H. Hsieh,C.W. Huang,M.C. Huang,C. Tai,J.P. Poivey,R. Rouvier,Y.S. Cheng 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.5
A synthetic strain of ducks (Anas platyrhynchos) was developed by introducing genes for long duration of fertility to be used as mother of mule ducklings and a seven-generation selection experiment was conducted to increase the number of fertile eggs after a single artificial insemination (AI) with pooled Muscovy semen. Reciprocal crossbreeding between Brown Tsaiya LRI-2 (with long duration of fertility) and Pekin L-201 (with white plumage mule ducklings) ducks produced the G0. Then G1 were intercrossed to produce G2 and so on for the following generations. Each female duck was inseminated 3 times, at 26, 29, and 32 weeks of age. The eggs were collected for 14 days from day 2 after AI. Individual data regarding the number of incubated eggs (Ie), the number of fertile eggs at candling at day 7 of incubation (F), the total number of dead embryos (M), the maximum duration of fertility (Dm) and the number of hatched mule ducklings (H) with plumage colour were recorded. The selection criterion was the breeding values of the best linear unbiased prediction animal model for F. The results show high percentage of exhibited heterosis in G2 for traits to improve (19.1% for F and 12.9% for H); F with a value of 5.92 (vs 3.74 in the Pekin L-201) was improved in the G2. Heritabilities were found to be low for Ie (h2 = 0.07±0.03) and M (h2 = 0.07±0.01), moderately low for Dm (h2 = 0.13±0.02), of medium values for H (h2 = 0.20±0.03) and F (h2 = 0.23±0.03). High and favourable genetic correlations existed between F and Dm (rg = 0.93), between F and H (rg = 0.97) and between Dm and H (rg = 0.90). The selection experiment showed a positive trend for phenotypic values of F (6.38 fertile eggs in G10 of synthetic strain vs 5.59 eggs in G4, and 3.74 eggs in Pekin L-201), with correlated response for increasing H (5.73 ducklings in G10 vs 4.86 in G4, and 3.09 ducklings in Pekin L-201) and maximum duration of the fertile period without increasing the embryo mortality rate. The average predicted genetic response for F was 40% of genetic standard deviation per generation of selection. The mule ducklings’ feather colour also was improved. It was concluded that this study provided results for a better understanding of the genetics of the duration of fertility traits in the common female duck bred for mule and that the selection of a synthetic strain was effective method of improvement.
AKARI mid-infrared slitless spectroscopic survey of star-forming galaxies at <i>z</i> ≲ 0.5
Ohyama, Y.,Wada, T.,Matsuhara, H.,Takagi, T.,Malkan, M.,Goto, T.,Egami, E.,Lee, H.-M.,Im, M.,Kim, J.H.,Pearson, C.,Inami, H.,Oyabu, S.,Usui, F.,Burgarella, D.,Mazyed, F.,Imanishi, M.,Jeong, W.-S.,Miya EDP Sciences 2018 Astronomy and astrophysics Vol.618 No.-
<P><I>Context.</I> Deep mid-infrared (MIR) surveys have revealed numerous strongly star-forming galaxies at redshift <I>z</I> ≲ 2. Their MIR fluxes are produced by a combination of continuum and polycyclic aromatic hydrocarbon (PAH) emission features. The PAH features can dominate the total MIR flux, but are difficult to measure without spectroscopy.</P><P><I>Aims.</I> We aim to study star-forming galaxies by using a blind spectroscopic survey at MIR wavelengths to understand evolution of their star formation rate (SFR) and specific SFR (SFR per stellar mass) up to <I>z</I> ≃ 0.5, by paying particular attention to their PAH properties.</P><P><I>Methods.</I> We conducted a low-resolution (<I>R</I> ≃ 50) slitless spectroscopic survey at 5-13 <I>μ</I>m of 9 <I>μ</I>m flux-selected sources (>0.3 mJy) around the north ecliptic pole with the infrared camera (IRC) onboard AKARI. After removing 11 AGN candidates by using the IRC photometry, we identify 48 PAH galaxies with PAH 6.2, 7.7, and 8.6 <I>μ</I>m features at <I>z</I> < 0.5. The rest-frame optical-MIR spectral energy distributions (SEDs) based on CFHT and IRC imaging covering 0.37-18 <I>μ</I>m were produced, and analysed in conjunction with the PAH spectroscopy. We defined the PAH enhancement by using the luminosity ratio of the 7.7 <I>μ</I>m PAH feature over the 3.5 <I>μ</I>m stellar component of the SEDs.</P><P><I>Results.</I> The rest-frame SEDs of all PAH galaxies have a universal shape with stellar and 7.7 <I>μ</I>m bumps, except that the PAH enhancement significantly varies as a function of the PAH luminosities. We identify a PAH-enhanced population at <I>z</I> ≳ 0.35, whose SEDs and luminosities are typical of luminous infrared galaxies. They show particularly larger PAH enhancement at high luminosity, implying that they are vigorous star-forming galaxies with elevated specific SFR. Our composite starburst model that combines a very young and optically very thick starburst with a very old population can successfully reproduce most of their SED characteristics, although we cannot confirm this optically think component from our spectral analysis.</P>
Sirunyan, A. M.,Tumasyan, A.,Adam, W.,Ambrogi, F.,Asilar, E.,Bergauer, T.,Brandstetter, J.,Brondolin, E.,Dragicevic, M.,Erö,, J.,Flechl, M.,Friedl, M.,Frü,hwirth, R.,Ghete, V. M.,Grossmann, J. Institute of Physics Pub 2017 The journal of high energy physics Vol.2017 No.8
<P>Searches are presented for heavy gauge bosons decaying into a top and a bottom quark in data collected by the CMS experiment at root s = 13TeV that correspond to an integrated luminosity of 2.2 and 2.6 fb 1 in the leptonic and hadronic analyses, respectively. Two final states are analyzed, one containing a single electron, or muon, and missing transverse momentum, and the other containing multiple jets and no electrons or muons. No evidence is found for a right-handed W' boson (W'(R)) and the combined analyses exclude at 95% confidence level W'(R) with masses below 2.4TeV if M-W'R >> M-vR (mass of the right-handed neutrino), and below 2.6TeV if M-W'R < M-vR. The results provide the most stringent limits for right-handed W' bosons in the top and bottom quark decay channel.</P>