MicroRNA-362 Inhibits Cell Proliferation and Invasion by Directly Targeting SIX1 in Colorectal Cancer

Jin’e Wan,Jian Yang,Cuixia Qiao,Xiaomei Sun,Aiting Di,Lize Zhang,Dandan Wang,Gang Zhao 연세대학교의과대학 2019 Yonsei medical journal Vol.60 No.5

Purpose: Colorectal cancer (CRC) is the third most common cancer in China and poses high morbidity and mortality. In recentyears, increasing evidence has indicated that microRNAs played important functions in the occurrence and development of tumors. The purpose of this study was to identify the biological mechanisms of miR-362 in CRC. Materials and Methods: Quantitative real-time PCR was carried out to assess the expression of miR-362 and SIX1. The Kaplan-Meier method was employed to evaluate the 5-year overall survival of CRC patients. The proliferative and invasive abilities of CRCcells were assessed by MTT and transwell assays. Results: miR-362 was significantly decreased in CRC tissues and cell lines, compared to the normal tissues and normal cells. Asignificant connection was confirmed between the overall survival of 53 CRC patients and low expression of miR-362. Downregulationof miR-362 inhibited the proliferation and invasion through binding to the 3'-UTR of SIX1 mRNA in CRC. Additionally, wediscovered that SIX1 was a direct target gene of miR-362 and that the expression of miR-362 had a negative connection with SIX1expression in CRC. SIX1 could reverse partial functions in the proliferation and invasion in CRC cells. Conclusion: miR-362 may be a prognostic marker in CRC and suppress CRC cell proliferation and invasion in part through targetingthe 3'-UTR of SIX1 mRNA. The newly identified miR-362/SIX1 axis provides insight into the progression of CRC.

Eight-year monitoring of the height growth and survivorship of seedlings of Pinus thunbergii Parl. planted with sand fence and bush hedge protection in a coastal sandy environment in Korea

Ewane Basil Ewane,이주형,이헌호 한국산림과학회 2016 Forest Science And Technology Vol.12 No.4

This study aimed to examine the effect of distance at three levels (5 m, 15 m, and 25 m) of ranges of plots landwards from a sand fence and bush hedge protection margin on the height growth and survival of seedlings of Pinus thunbergii Parl. planted in a coastal sandy environment in Uljin, East Sea area of Korea. The purpose of the study was to investigate height growth and survival with distance from a bush hedge/sand beach boundary of pine seedlings in a coastal forest plantation. Seedling height growth and survivorship were measured annually in nine plots, with three plots each selected from three ranges of plots at distances of 5 m, 15 m, and 25 m landward from the bush hedge/sand beach boundary from 2007 to 2014, 8 years after planting. Annual averages in seedling height growth significantly reduced in the 2nd and 3rd years of growth but appreciably stabilized from the 4th to 8th year, indicating that the 2nd and 3rd years of seedling growth and survival in the study site were the most critical. We found no statistically significant differences in height growth and survivorship of the planted seedlings between the three ranges of plots at distances of 5 m, 15 m, and 25 m landward from the bush hedge/sand beach boundary in the microsite. This suggested that seedling height growth and survivorship were not significantly affected by the distance factor. This was attributed to the protective and stabilization functions of the bush hedges and sand fence barrier, which reduced near-surface wind damage to seedlings and fostered a relatively uniform height growth and survival of the planted seedlings in the microsite. Furthermore, wind speed (P D 0.014) and soil moisture at 12 cm depth (P D 0.014) were the most significant of the selected factors limiting the height growth of the seedlings in the study area, as they accounted for 94.4% (R2 D 0.944) of the insignificant variability associated with the distance factor. The results provide supportive evidence that sand fence and bush hedge micro-windbreaks can be reliably used to protect, stabilize, and sustain relatively uniform height growth and survival of seedlings of P. thunbergii planted in a windy coastal sandy environment and in other locations with similar soil and climatic conditions.

Dermal Exposure Associated with Occupational End Use of Pesticides and the Role of Protective Measures

Ewan MacFarlane,Renee Carey,Tessa Keegel,Sonia El-Zaemay,Lin Fritschi 한국산업안전보건공단 산업안전보건연구원 2013 Safety and health at work Vol.4 No.3

Background: Occupational end users of pesticides may experience bodily absorption of the pesticide products they use, risking possible health effects. The purpose of this paper is to provide a guide for researchers, practitioners, and policy makers working in the field of agricultural health or other areas where occupational end use of pesticides and exposure issues are of interest. Methods: This paper characterizes the health effects of pesticide exposure, jobs associated with pesticide use, pesticide-related tasks, absorption of pesticides through the skin, and the use of personal protective equipment (PPE) for reducing exposure. Conclusions: Although international and national efforts to reduce pesticide exposure through regulatory means should continue, it is difficult in the agricultural sector to implement engineering or system controls. It is clear that use of PPE does reduce dermal pesticide exposure but compliance among the majority of occupationally exposed pesticide end users appears to be poor. More research is needed on higher-order controls to reduce pesticide exposure and to understand the reasons for poor compliance with PPE and identify effective training methods.

A User View of Convergence

Ewan Sutherland 정보통신정책연구원 2003 세미나및공청회 Vol.2003 No.

Economic MAD as Middle Powers’ Strategic Tool in the Great Power Rivalry

Ewan A. Smith,Shin-wha Lee 한국국제정치학회 2022 The Korean Journal of International Studies Vol.20 No.2

Convergence in Africa and Europe

Ewan Sutherland 한국방송학회 2006 한국방송학회 세미나 및 보고서 Vol.- No.-

Afghanistan: Is Failure an Option?

Martin Ewans 동아시아재단 2008 Global Asia Vol.3 No.1

OccIDEAS: An Innovative Tool to Assess Past Asbestos Exposure in the Australian Mesothelioma Registry

MacFarlane, Ewan,Benke, Geza,Sim, Malcolm R.,Fritschi, Lin Occupational Safety and Health Research Institute 2012 Safety and health at work Vol.3 No.1

Malignant mesothelioma is an uncommon but rapidly fatal disease for which the principal aetiological agent is exposure to asbestos. Mesothelioma is of particular significance in Australia where asbestos use was very widespread from the 1950s until the 1980s. Exposure to asbestos includes occupational exposure associated with working with asbestos or in workplaces where asbestos is used and also 'take-home' exposure of family members of asbestos exposed workers. Asbestos exposure may also be nonoccupational, occurring as a consequence of using asbestos products in non-occupational contexts and passive exposure is also possible, such as exposure to asbestos products in the built environment or proximity to an environmental source of exposure, for example an asbestos production plant. The extremely long latency period for this disease makes exposure assessment problematic in the context of a mesothelioma registry. OccIDEAS, a recently developed online tool for retrospective exposure assessment, has been adapted for use in the Australian Mesothelioma Registry (AMR) to enable systematic retrospective exposure assessment of consenting cases. Twelve occupational questionnaire modules and one non-occupational module have been developed for the AMR, which form the basis of structured interviews using OccIDEAS, which also stores collected data and provides a framework for generating metrics of exposure.

Dermal Exposure Associated with Occupational End Use of Pesticides and the Role of Protective Measures

MacFarlane, Ewan,Carey, Renee,Keegel, Tessa,El-Zaemay, Sonia,Fritschi, Lin Occupational Safety and Health Research Institute 2013 Safety and health at work Vol.4 No.3

Background: Occupational end users of pesticides may experience bodily absorption of the pesticide products they use, risking possible health effects. The purpose of this paper is to provide a guide for researchers, practitioners, and policy makers working in the field of agricultural health or other areas where occupational end use of pesticides and exposure issues are of interest. Methods: This paper characterizes the health effects of pesticide exposure, jobs associated with pesticide use, pesticide-related tasks, absorption of pesticides through the skin, and the use of personal protective equipment (PPE) for reducing exposure. Conclusions: Although international and national efforts to reduce pesticide exposure through regulatory means should continue, it is difficult in the agricultural sector to implement engineering or system controls. It is clear that use of PPE does reduce dermal pesticide exposure but compliance among the majority of occupationally exposed pesticide end users appears to be poor. More research is needed on higher-order controls to reduce pesticide exposure and to understand the reasons for poor compliance with PPE and identify effective training methods.

Correlation between above-ground and below-ground biomass of 13-year-old Pinus densiflora S. et Z. planted in a post-fire area in Samcheok

김도현,김재희,박진화,Ewane Basil Ewane,이도형 한국산림과학회 2016 Forest Science And Technology Vol.12 No.3

This study was carried out in order to explore and compare the correlation between each measured item of the aboveground and below-ground biomass of 13-year-old Pinus densiflora S. et Z. and prepare regression equations to identify differences of early growth characteristics on each slope aspect in the stand. The south-facing slope showed the highest above-ground and below-ground biomass, followed by the north-facing slope and ridge area. The root collar diameter showed the highest correlation between the biomass of the above-ground part and the below-ground part. In the linear regression equation on the root collar diameter and the biomass of the above-ground part and the below-ground part, the growth gradient was high in the south-facing slope and north-facing slope and low in the ridge area such that the biomass in the ridge area may well continue to be low in the future as the trees grow. The result of the study demonstrated that the differences in the growth environments associated with differences in the slope aspect had a significant influence on the early growth of trees. The results of this study further manifested the importance of the concept of slope direction in artificial forestation and could be used as preliminary data to support artificial forestation measures in the future.