씨름선수에서 체중 감량과 회복기의 Leptin과 PAI-1 변화

김용운,도경오,권태동,박덕일,장응찬,박소영,김종연,이석강 대한스포츠의학회 2000 대한스포츠의학회지 Vol.18 No.2

Obesity which is defined as accumulation of excess body fat, is central factor of insulin resistance syndrome. Recently, it is revealed tat adipose tissue is not simply an energy storage organ but it also secretes a variety of molecules which affect the metabolism of the whole body, those are leptin and plasminogen activator inhibitor-1(PAI-1). Therefore, leptin and PAI-1 are increased in the obese state. Leptin regulates energy homeostasis and satiety and PAI-1 regulates fibrinolytic system. For these reasons, elevated levels of leptin and PAI-1 are considered as link factors between obesity and insulin resistance syndrome. However, the exact regulating mechanism for serum levels of leptin and PAI-1 is not fully understood yet. In this study, to evaluate the regulating mechanisms of serum levels of leptin and PAI-1 according to the weight changes, we measured leptin, PAI-1, physical, metabolic, and endocrine parameters during 10 days of weight reduction and 10 days of regain period in 7 young athletes. The mean body weight change was -4.7 kg(5.0%) in the weight reduction period and -2.4 kg(2.5%) in the weight regain period compared to baseline value. Baseline level of leptin in athletes was 1.7±0.66 ng/ml, which was significantly correlated with body weight, BMI, percent body fat, body fat mass, triglyceride, insulin, and PAI-1. Baseline level of PAI-1 in athletes was 16.6±5.26 ng/ml, which was significantly correlated with body weight, BMI, triglyceride, insulin, and leptin. Leptin was decreased to 0.7±0.39(44% of the basaline value) in the weight reduction period, and increased to 1.9±0.64(119% of the baseline value) in the regain period. PAI-1 was decreased to 7.4±2.72(44% of the basaline value) in the weight reduction period, and increased to 22.8±7.33(138% of the baseline value) in the regain period. The changes of leptin during weight reduction period were significantly correlated with the changes of insulin(r=0.890, P<0.01) and triglycerides(r=0.874, P<0.01). The changes of PAI-1 during weight reduction period were significantly correlated with the changes of FFA(r=0.889, P<0.01) and triglycerides(r=0.869, P<0.05). The changes of both leptin and AAI-1 during weight regain period were significantly correlated with the changes of insulin(r=0.755 and 0.849, P<0.05, respectively). In summary, these results suggest that serum levels of leptin and PAI-1 were affected by weight cycling, the percentages of change were more greater than that of weight change, and rebound phenomena were occurred during weight regain period.

Increased 11β-hydroxysteroid dehydrogenase type 1 contributes to barrier dysfunction in aged skin

( Beom Jun Kim ),( Noo Ri Lee ),( Chung Hyeok Lee ),( Young Bin Lee ),( Solam Lee ),( Hyun Jee Hwang ),( Eunjung Kim ),( Eung Ho Choi ) 대한피부과학회 2019 대한피부과학회 학술발표대회집 Vol.71 No.2

Background: 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) converts inactive cortisone into cortisol, an active form, and is expressed by several tissues including the skin. Excessive active glucocorticoid (GC) deteriorates skin barrier function. Objectives: To find out if 11β-HSD1 affects on the barrier function in aged skin. Methods: We have performed human and in vivo studies. We measured cortisol in stratum corneum (SC) and oral epithelium of the elderly and young. Hairless mice were used for 11β-HSD1 immunohistochemistry staining of skin and measuring skin barrier function and serum cytokines. 11β-HSD1 knock-out (KO) mice and its wild-type were used for measuring lipid synthesis related enzyme. Results: Cortisol levels were elevated in SC and oral epithelium of the elderly rather than young. The 11β -HSD1 expression was increased in immunohistochemistry stain of aged mice skin. Aged mice showed decreased transepidermal water loss (TEWL) and SC hydration, and increased SC integrity than young. Serum inflammatory cytokines including interleukin-1α, -4, -10, -31 and tumor necrosis factor-α were increased in aged mice than young. The expressions of lipid synthesis related enzymes in epidermis were elevated in KO mice and topical 11β -HSD1 inhibitor applied mice. Conclusion: 11β-HSD1 expression is elevated in aged skin. It increases active GC and then deteriorates skin barrier function.

Removal of Cochlodinium polykrikoides using a novel material produced from sediment - A field study

Young一Chae Song,S. Sivakumar,Sung-Chung Ko,Eung-Ju Hwang,Qtae Jo 한국항해항만학회 2007 한국항해항만학회 학술대회논문집 Vol.2 No.추계

The present study was conducted in three fields at Namhae to examine the removal efficiency of organic free, heavy metal immobilized sediment on Cochlodinium polykrikoidesand on sea water quality. The present study results concluded that removal efficiency was depends on the initial number of red tide cells. There was no drastic change in the sea water quality after sediment spray. For the comparison of effectiveness of betonite, zeolite and lime were mixed with sediment did not show any marked difference in removal. Finally, the present study evaluated 5Og/m² sediment is Sufficient to remove 100% cells density, even though various environmental factors are interfering the mechanism.

미오신 II에 의한 신경 성장추의 액틴 다이내믹스 조절

신은영, 김응국 충북대학교 의과대학 충북대학교 의학연구소 2012 忠北醫大學術誌 Vol.22 No.1

연구목적: 미오신 II는 액틴과 결합해서 세포의 다양한 활성, 수축, 이동, 부착 등을 조절한다. Rho G 단백질도 유사한 역할을 수행한다. 따라서 미오신 II와 Rho G 단백질의 활성 조절은 밀접하게 연 관되어 있다. 본 연구에서는 신경 성장추를 모델로 이 두 개의 단백질이 어떻게 액틴 다이내믹스를 조 절하는가에 관한 연구를 수행코자 한다. 대상 및 방법: 신경 성장추는 쥐의 해마세포를 초대배양하여 확보한다. 미오신 II에 대한 저해제인 blebbistatin (BBS)을 처리한 후 신경 성장추의 액틴 골격의 변화를 관찰한다. 또한 신경세포의 신경 돌기 및 성장추의 확장을 촉진하는 인자인 신경성장인자를 처리하고 미오신 II의 인산화를 면역블롯과 면역화학법을 통해 관찰한다. 결과: 초대배양한 해마세포의 신경 성장추에 BBS를 처리하면 30분 내에 질서정연한 액틴 골격이 무 질서한 액틴 골격으로 변한다. BBS를 배양액에서 제거하면 서서히 원래의 형태로 전환되는 것으로 미 루어, BBS에 의한 액틴 골격의 변화는 가역적인 반응으로 추정된다. 신경성장인자를 처리하면 BBS에 의해 유도되는 급격한 변화는 보이지 않았다. 그러나 미오신 II의 인산화를 면역블롯과 면역염색을 통 해 확인할 수 있었다. 결론: 미오신 II는 인산화를 통해 Rho G 단백질의 활성과 신경 성장추의 액틴을 재구성하는 것으로 보인다.

Rho GTPase와 미오신 활성의 상관관계 조사

김응국, 신은영 충북대학교 의과대학 충북대학교 의학연구소 2012 忠北醫大學術誌 Vol.22 No.1

연구목적 : 본 연구는 PC12 세포에서 nerve growth factor (NGF) 처리에 의해 Rho GTPase Rac이 활성화 되었을 때 미오신 II와 guanine nucleotide exchange factor (GEF) PIX의 상호작용을 관찰하고자 하였다. 재료 및 방법 : PC12 세포는 10% 혈청이 포함된 DMEM에서 배양하였다. NGF는 처리 전 24 시간 동안 혈청을 제거하였다. NGF 20 ng/ml를 표기한 시간대로 처리한 후 단백질 용출액을 처리하여 세포균질액을 얻었다. 이 과정을 통해 얻은 세포균질액은 PBD-His beads로 pulldown assay를 실시하여 Rac의 활성화를 검증하였다. 또한 anti-PIX antibody로 면역침전한 후 미오신 II 항체를 이용하여 결합여부를 확인하였다. 결과 : Rac의 활성은 NGF 처리 후 5분부터 20분까지 꾸준히 증가하였으며 30분에는 감소 하는 경향을 나타냈다. 뿐만 아니라 PIX와 미오신 II와의 결합은 10분부터 감소하기 시작하 여 20-30분에는 결합한 두 단백질의 양이 상당히 감소함을 확인할 수 있었다. 결론 : 이상의 결과로부터 NGF 처리에 의해 Rac이 활성화 되는 것과 PIX와 미오신 II의 결합이 해리되는 시간대가 일치한다는 것을 알 수 있었다. 이는 NGF 처리가 PIX와 미오신 II의 결합 상태를 해리시켜 PIX의 GEF 활성을 유도한다는 것을 설명하고 있다.

ADH와 Morphine 및 Alcohol의 同時投與가 흰쥐 肝組織內 Cytochrome P-450 b_5, p-nitroanisole-O-demethylation 및 AAF hydroxylation에 미치는 影響

洪永淑,金龜子,成樂應 梨花女子大學校 韓國生活科學硏究院 1985 韓國生活科學硏究院 論叢 Vol.35 No.-

Chronic morphine administration to adult male rats has long been known to change hepatic cytochrome P-450 content and its dependent mixed function oxidase activity. More recently, acute treatment of mature male rats with a dose of morphine higher than that used chronically also has been reported to change their hepatic cytochrome P-450 In the present study, the effect of morphine and alcohol by concomitant administration of ADH on the cytochrome P-450, b_5, p-nitroanisole-O-demethylation and AAF-hydroxylation in rat liver microsomes were studied. The results are as follows; 1. The contensts of cytochrome P-450, and b_5 were increased in the liver microsomes from morphine and ADH-treated rats, whereas they were decreased in liver microsome from alcohol and ADH-treated rats. 2. After administration of morphine and ADH rats, activity p-nitroanisole-O-demethylase, mixed function oxidase system were increased, whereas they were decreased after administration of alcohol and ADH rats. 3. After administration of morphine and ADH rats, the formation of lipid peroxides were decreased, whereas they were increased after administration of alcohol and ADH rats. No correlation was found between lipid peroxidation and formation of lipid peroxidases, which were induced by morphine and ADH cytochrome P-450 content. There might be another enzyme catalyzing lipid peroxidation other than cytochrome P-450. 4. The administration of morphine and ADH increased AAF-ring-hydroxylation, compared to ADH group.

주사형 정전용량 현미경의 개발

金應珪,權寧島 大田産業大學校 1996 한밭대학교 논문집 Vol.13 No.0201

In this study, a scanning capacitance microscopy(SCaM) by stage driving is proposed and presented some of the experimental results. SCaM is a microscopy which scans a surface of materials mechanically in two or two point five dimensions by a capacitance probe with a few tenth ㎛ size tip, and display images of the surface shape or capacitive distribution. The present target of the SCaM is 0.1㎛ resolution power which exceeds that of optical microscopy. This will become a powerful tool for inspecting ULSI pattern etched by X-ray, biological data, etc. The experimental system is composed based on a VHD video disk which captures the capacitance changes of the video disk surface and converts it into video signal.

동해안 연안어항 해수중의 COD 및 중금속 함량에 관한 연구

김영철,주충열,신응배 漢陽大學校 環境工學硏究所 1999 環境科學論文集 Vol.20 No.-

동해 연안 일부어항의 해수에 대한 수질환경 자료로 DO, ?? 총질소, 총인 그리고 납, 카드뮴, 크롬, 비소 및 수은 등의 중금속을 측정·조사하였다. 측정결과, DO 5.1∼6.2㎎/l, ?? 0.9∼2.7㎎/l, 납 trace∼0.054㎎/l, 카드뮴 trace∼0.004㎎/l, 비소 trace∼0.002㎎/l, 그리고 수은 trace로 나타났다. 이러한 결과로부터 오염의 정도를 판단하기는 어려우나 환경부가 정한 해역의 수질환경기준과 비교하여 볼 때 중금속으로 인한 오염문제는 크게 우려할 수준이 아니나 일부어항의 ?? 용존산소, 총질소, 총인의 농도로 판단할 때 Ⅱ등급 또는 Ⅲ등급 수질로 나타났다. 이들 지역이 선박이 정착하거나 출입하는 어항임을 감안할 때 비교적 양호한 결과인 것으로 분석된다. The seawater was sampled from the East Coast and the contents of DO, ?? total nitrogen, total phosphates, and heavy metals(Pb, Cd, As, Hg) in the water were measured and analyzed. DO 5.1∼6.2㎎/l, ??0.9∼2.7㎎/l, total nitrogen<0.131㎎/l, total phosphate <0.019㎎/l, Pb <0.0542㎎/l, Cd<0.0038㎎/l, As<0.0016㎎/l, and Hg(trace) were detected by the analytical techniquies such as UV-visible spectrometry and atomic absorption spectrometry. The results shows that heavy metal contamination of the East Coast is equivalent to the detection limits of the first grade seawater. But, the amount of DO, ?? total nitrogen, total phosphates shown above indicates that the seawater in the East Coast is of second or third quality. In conclusion, the samples cannot be justified as the first quality. Considering that the sampling sites were seaports, the seawater in the East Coast is not seriously contaminated.

작물의 음향 신호에 대한 반응 분석에 관한 연구

이근영,허태원,김응성,이재원 成均館大學校 科學技術硏究所 1996 論文集 Vol.47 No.2

The puper studies the relationship between plants and audio signals. Responses of plant are transformed to electrical signals and digitized to process them on computer. Variance and mean are used for spatial domain analysis. and Fourier transform is used for frequency responses analysis as tools. We used celery cabbage, cucumber, parsley and onions as experimental plants. With this experiments, we found out that specific plants are sensitive to specific music signals and external stimulus.

Filamentous fungi Aspergillus species에서 Phytase 고발현 균주의 선별

이응석,함영태 中央大學校 食糧資源硏究所 1997 食糧資源硏究所 論文集 Vol.18 No.1

Phytase production of Aspergillus species were influenced by nitrogen source. Ammonium ions, especially ammonium phosphate (NH4H2PO4), were markedly superior to nitrate for the Phytase production. For the isolation of phytase gene, the culture of Aspergillus species (A. oryzae) were maintained on PSM media containing ammonium phosphate. After 4 days, mycellia were harvested by centrifugation and isolated total RNA. cDNAs were synthesized from total RNA by RT-PCR. The Phytase gene was amplified with the primers designed from A. ficuum sequence. 1.2 and 2.0 kb of the amplified DNA fragments by secondary PCR and subcloned in pT7Blue-T vector. Aspergillus species (A. oryzae and A. ficuum) were treated with 254㎚ of UV light for the screening of high phytase producing mutant strains, comparative to wild type on PSM media, containing ammonium phosphate. Two UV mutant strains in A. oryzae and three, in A. ficuum were isolated. These mutants are 1.5 to 2.0 times better phytase-production than Wild type.