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      • KCI등재

        대전시 노은 도매시장 채소류의 농약 잔류 실태 및 식이섭취량 추정

        한국탁,이규승,이은경,이용재,고광용,원동준,이정원,권순덕 한국환경농학회 2003 한국환경농학회지 Vol.22 No.3

        대전시 노은 도매시장에서 채취한 6종의 채소류 중 농약잔류량을 조사한 후 이에 대한 위해성 평가를 실시하였다. 전체 100건의 분석시료 중 농약이 검출된 시료의 비율은 46.0%이었고, 잔류허용기준 초과비율은 6.0% 이었다. 검출비율이 높은 시료는 상추(85.0%), 깻잎(80.0%), 오이(60.0%) 순이었고, 상추 3건, 깻잎 3건이 허용기준을 초과하였다. 살균제는 dicar-boximide계, 유기염소계, azole계 농약, 살충제는 유기인계, 합성 pyrethroid계 농약이 많이 검출되었다. 검출빈도가 높은 성분은 procymidone, chlorpyrifos, chlorothalonil, cypermethrin, EPN의 순이었다. 위해성을 평가하기 위해서 각 농작물에 대한 농약의 추정 섭취량과 ADI를 비교한 결과, 허용기준을 초과한 깻잎의 bitertanol, triflumizole, iprobenphos가 다른 성분에 비하여 높은 비율을 보였다. 그러나 각 농산물의 식이섭취량이 적어 ADI에 대한 추정섭취량의 비율이 0.46%이하로 나타나 그 영향이 매우 낮은 것으로 판단되었다. Pesticide residues in some vegetables collected at Noeun wholesale market in Daejeon were surveyed and assessed their risk In 100 samples, the detection rate of pesticide was 46.0% and the rate exceed MRL was 6.0%. Commodities showing high detection rate were lettuce(85.0%), perilla leaf(80.0%) and cucumber(60.0%). Dicarboximide, organochlorine, and azole fungicides and organophosphorus and pyrethroid insecticides were detected. Detection frequency of pesticide was in the order of procymidone, chlorpyrifos, chlerothalonil, cypermethrin and EPN. When the estimated intake amount of the pesticides were compared with ADI to assess their risk, bitertanol, triflumizole and iprobenphos in perilla leaf were higher rate than the other vegetables. However the total amounts of intake of pesticides were estimated to less than 0.46% of ADI.

      • 직장인의 스포츠활동 참가와 주관적 행복감의 관계

        임준댁,탁은정 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.2

        The Purpose of this study to investigate the relationship of sports activity participation to subjective euphoria of office workers. To achieve this purpose, the subjects were selected by cluster random sampling of adults, in Daejon in 2002 at present. There were 273 male, 215 female used for the analysis. The total was 489. Data were collected by employing a questionnaire. The variables of this analysis were demographic characteristics, sports activity participation, subjective happiness(positive affect scale and negative affect scale). The questionnaire for Life sathisfaction had been developed by Diener. Emmons, Lersen & Griffin(1992) and standardized in Korean language by Lee Jong kil(1992). The questionnaire for Positive and Negative Affectby Watson, Clark & Tellgen(1988) and standardized in Korean language by Lee Ju Sung(1991). The collected data analyzed statistic program SPSS Windows 10.0 Version. The statistical analysis are descriptive statistics analysis, multiple regression analysis and analysis of covariance. From the analyses of the data, this study reached he following conclusions: First, subjective euphoria is partially different by sports activity participation among employees. Second, degree of sports activity participation is positively influence on subjective euphoria.

      • 播種期 移動에 따른 차풀의 生育反應, 收量 및 粗成分 變化

        玄京卓,趙南棋,吳恩敬,高志棅,趙英一 濟州大學校 亞熱帶農業硏究所 1999 亞熱帶農業硏究 Vol.16 No.-

        ABSTRACT This study was conducted to determine influence of planting date, seeding rate and phosphate rate on main growth, characters, yield and chemical composition of senna from March 11, 1998 to December in Cheju Province. Days to flowering was lessened as planting date was delayed. The plant height of senna was the longest at 31 March planting (99cm) than at the other planting date. Stem diameter, number of leaves were increased at 31 March but number of branches was not affected by planting date. And number of withering leaves were increased as planting date was earlier. Fresh forage yield per lOa, for senna was the greatest (4,660kg) at 31 March planting, the second was 4,397kg at 1 April planting and the reduced the 3,473kg at 11 March planting. Also dry matter yield per lOa, crude protein yield and total digestable nutrient (TDN) yield was nearly the same tendency. Crude protein, crude fat percent was the greastest at 11 March planting and than decreased with further increased at planting date. Crude ash and crude fiber percent was decreased tendency as planting date was delayed. Nitrogen free extract (NFE), TDN percent was increased tendency as planting date was delayed. SPAD reading value of leaves had an efficiency increased as planting date was delayed.

      • 신경병증성 통증 모델쥐 뒤뿌리신경절의 Nitric Oxide Synthase 아형 및 NADPH-diaphorase 발현에 관한 연구

        정성균,김수일,양은진,류택근,권오유,박경란,이영호,김원식 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.2

        Injury to peripheral nerve somtimes lead to neuropathic pain. Nitric oxide(NO) is increased in the dorsal root ganglia (DRG) of neuropathic pain models, and L-NAME, a nitric oxide synthase (NOS) inhibitor, reduces neuropathic pain. However, it is not known that underlying mechanism of NO involvement in development and maintanence of neuropathic pain. It was found that there were three NOS isoforms-nNOS, eNOS, and iNOS. It is also necessary to investigate NOS isoforms in neuropathic pain. The aim of this study is to investigate NOS isoforms and NADPH-diaphorase expressions and underlying mechanism of NO involvement in neuropathic pain. The 5^th lambar (L5) DRG were obtaind after tight ligation of L5 an L6 spinal nerves for 7 days. Toluidine blue stain, immunohistochemistry for nNOS, eNOS, and iNOS, and NADPH-diaphorase histochemistry were performed. All specimens were observed under light microscope. nNOS expression was increased significantly in the cytoplasm of the DRG of ligated side at 7 day post-ligation (7D PL). eNOS expression was also increased in the blood vessel, a few neurons or satellite cells, and cell membrane of Schwann cells in the DRG of ligated side at 7D PL. iNOS expression was increased in a few blood vessels and cytoplasm of small neurons in the DRG of ligated side at 7D PL. NADPH-diaphorase expression was increased significantly in the neurons of the DRG of ligate side at 7D PL, but NADPH-diaphorase expressions were weak or absent in the blood vessel and Schwann cell in the contralateral DRG and the DRG of ligated side at 7D PL. It is suggested that NOS isoforms may be closely related to development and mantanence of neuropathic pain in order of nNOS>eNOS>iNOS.

      • KCI등재

        악교정 수술 후 발생하는 신경회복에 대한 연구Ⅰ

        이동근,조이수,민승기,오승환,정창주,이은택 대한악안면성형재건외과학회 2001 Maxillofacial Plastic Reconstructive Surgery Vol.23 No.2

        Dysfunction of the inferior alveolar nerve indicated by various degree of numbness of the lower lip and chin is one of the few drawbacks of mandibular osteotomy, especially Bilateral Sagittal Split Ramus Osteotomy(BSSRO) and genioplasty. Although it has been recorded throughout the history of this techniques, it is true etiology poorly understood. In this study, 22 consecutive patients under class III malocclusiion impression and undergoing orthognathic surgery(BSSRO only 11 case, BSSRO with genioplasty 11 case) were studied using 4 neurosensory test(static light touch, directional discrimination, two-point discrimination, pin pressure nociception) with post OP 1 week, 2 weeks,4 weeks, 8 weeks, 12 weeks, 24 weeks, On control group, 10 members without trauma and nerve damage history, nerve test was accomplished. We concluded majority of patients return of sensation during post operative 24 weeks. Althought immediate nerve deficit are 92.2%, 97.2% 88.9% these are recovered to 25%, 35.72%, 10.71% at 24 weeks. Nerve recovery rate increased prominently between post 4 weeks and 8 weeks. There is no statistically difference about neurosensory deficit among the chin area. Neurosensory deficit more severe when the BSSRO with genioplasty group than the only BSSRO group. Immediate neurosensory deficit is larger left side than right side but after 6 months, there is no significantly difference between left side and right side. Static light touch and pin pressure nociception are more sensitive method of neurosensory deficit than two point discrimination.

      • KCI등재
      • T-cell-restricted intracellular antigen 1 facilitates mitochondrial fragmentation by enhancing the expression of mitochondrial fission factor

        Tak, Hyosun,Eun, Jung Woo,Kim, Jihye,Park, So Jung,Kim, Chongtae,Ji, Eunbyul,Lee, Heejin,Kang, Hoin,Cho, Dong-Hyung,Lee, Kyungbun,Kim, Wook,Nam, Suk Woo,Lee, Eun Kyung Macmillan Publishers Limited, part of Springer Nat 2017 Cell death and differentiation Vol.24 No.1

        <P>Mitochondrial morphology is dynamically regulated by the formation of small fragmented units or interconnected mitochondrial networks, and this dynamic morphological change is a pivotal process in normal mitochondrial function. In the present study, we identified a novel regulator responsible for the regulation of mitochondrial dynamics. An assay using CHANG liver cells stably expressing mitochondrial-targeted yellow fluorescent protein (mtYFP) and a group of siRNAs revealed that T-cell intracellular antigen protein-1 (TIA-1) affects mitochondrial morphology by enhancing mitochondrial fission. The function of TIA-1 in mitochondrial dynamics was investigated through various biological approaches and expression analysis in human specimen. Downregulation of TIA-1-enhanced mitochondrial elongation, whereas ectopic expression of TIA-1 resulted in mitochondria fragmentation. In addition, TIA-1 increased mitochondrial activity, including the rate of ATP synthesis and oxygen consumption. Further, we identified mitochondrial fission factor (MFF) as a direct target of TIA-1, and showed that TIA-1 promotes mitochondrial fragmentation by enhancing MFF translation. TIA-1 null cells had a decreased level of MFF and less mitochondrial Drp1, a critical factor for mitochondrial fragmentation, thereby enhancing mitochondrial elongation. Taken together, our results indicate that TIA-1 is a novel factor that facilitates mitochondrial dynamics by enhancing MFF expression and contributes to mitochondrial dysfunction.</P>

      • SCIESCOPUSKCI등재

        Synergistic Antibacterial Activity of an Active Compound Derived from Sedum takesimense against Methicillin-Resistant Staphylococcus aureus and Its Clinical Isolates

        ( Eun-tak Jeong ),( Seul-ki Park ),( Du-min Jo ),( Fazlurrahman Khan ),( Tae Ho Choi ),( Tae-mi Yoon ),( Young-mog Kim ) 한국미생물 · 생명공학회 2021 Journal of microbiology and biotechnology Vol.31 No.9

        There are a growing number of reports of hospital-acquired infections caused by pathogenic bacteria, especially methicillin-resistant Staphylococcus aureus (MRSA). Many plant products are now being used as a natural means of exploring antimicrobial agents against different types of human pathogenic bacteria. In this research, we sought to isolate and identify an active molecule from Sedum takesimense that has possible antibacterial activity against various clinical isolates of MRSA. NMR analysis revealed that the structure of the HPLC-purified compound was 1,2,4,6-tetra-Ogalloyl- glucose. The minimum inhibitory concentration (MIC) of different extract fractions against numerous pathogenic bacteria was determined, and the actively purified compound has potent antibacterial activity against multidrug-resistant pathogenic bacteria, i.e., MRSA and its clinical isolates. In addition, the combination of the active compound and β-lactam antibiotics (e.g., oxacillin) demonstrated synergistic action against MRSA, with a fractional inhibitory concentration (FIC) index of 0.281. The current research revealed an alternative approach to combating pathogenesis caused by multi-drug resistant bacteria using plant materials. Furthermore, using a combination approach in which the active plant-derived compound is combined with antibiotics has proved to be a successful way of destroying pathogens synergistically.

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