저장조건에 따른 Leucaena 화분의 발아력

정병룡,박의호 대구대학교 과학기술연구소 1996 科學技術硏究 Vol.3 No.1

Leucaena is the common name for Leucaena leucocephala which is a tropical leguminous tree with a potential value as a source of wood production, high quality forage, and revegetation of hillslopes. In spite of the plant's value, both the differences of pod setting ratio among Leucaena species and the differences of flowering period limited breeding of high quality species of Leucaena plant. To overcome these problems this study was conducted to find the possibility of long term storage of Leucaena pollen by using vacuum and low temperatures. Pollen viability decreased after 1.5 days after storage at room temperature. The same trend was observed for the pollen stored at refrigerator (91℃). Pollen viability, however, was maintained longer with decreasing storage temperature to that of freezer (-17℃). Vacuum treatment also helped to maintain pollen viability either at room or refrigerator temperatures. The ratio of exploded pollen showed the same pattern as the percentage of normally germinated pollen stored under different conditions except the pollen stored at refrigerator. Average tube lengths of germinated pollens were 4.3 and 3.6 mm for the pollen stored at freezer and refrigerator with vacuum, respectively. In conclusion, the viability of Leucaena pollen could be extended by storing under vacuum at freezing temperatures.

혈관내피세포-U937세포의 부착에 미치는 Eicosapentaenoic Acid의 효과

김은지,전재은,임현주,차성철,정의룡,조용근,조용근 경북대학교 병원 1998 경북대학교병원의학연구소논문집 Vol.2 No.1

연구배경: 역학적연구에서 어류의 섭취가 많은 집단에서 관상동맥질환의 빈도가 낮은 것이 보고되었고, 이것은 어류에 많이 함유된 불포화지방산(polyunsaturated fatty acid) 특히 eicosapentaenoic acid 때문으로 생각되어지고 있다. 본 연구에서는 동맥경화증 발생의 초기단계로 여겨지는 단핵구와 혈관내피세포 사이의 adhesion에 미치는 다불포화지방산의 영향을 in vitro system에서 관찰하고자 하였다. 방 법: 제대정맥에서 얻은 혈관내피세포를 배양하여 96 well에 옮기고 25, 50, 100, 200 ㎛ 농도의 eicosapentaenoic acid(EPA)를 6시간 동안 처리한 U937세포를 부착시켜 adhesion assay를 시행하였다. 혈관내피세포를 자극하기 위해서는 lipopolysaccharide(LPS)를 well당 100 ng씩을 투여하였다. 음성대조군으로는 LPS로 자극하지 않은 혈관내피세포에 EPA처리를 하지 않은 U937세포로 adhesion assay한 성적을 사용했고, 양성대조군으로는 LPS로 자극한 혈관내피세포에 EPA처리를 하지 않은 U937세포로 adhesion assay한 성적을 사용했다. 그리고 EPA(3.3 mM)을 5㎛ CuSO4 및 300 ㎛ asscorbic acid와 혼합하여 섭씨 37도에서 16시간 두어 산화-EPA를 만들어 같은 실험을 반복하였다. 결 과: U937세포에 25, 50, 100, 200 ㎛의 EPA로 6시간 처리하였을 때 U937세포의 생존율은 99% 이상이었다. 혈관내피세포를 LPS로 자극했을 때 adhesion assay에서 혈관내피세포에 붙는 U937세포의 수는 현저히 증가하였다. 그러나 EPA를 U937세포에 25, 50, 100, 200 ㎛의 농도로 6시간 처리 후에 lipopolysac-charide를 처리한 제대정맥 내피세포에 부착(adhesion)시켰을 때 처리한 EPA의 각 농도에 따른 부착된 U937세포의 수는 통계적으로 유의한 차이를 나타내지 못했다. EPA처리가 부착된 U937세포의 수에 영향을 미치지 못하였다. 결 론: EPA는 U937세포에 영향을 미쳐 혈관내피세포-U937 세포부착을 감소시키지는 못하였다. 따라서 EPA에 의한 혈관내피세포-단핵구 부착 억제 효과는 EPA의 단핵구에 미치는 영향에 의하지는 않는 것으로 생각되나 추후 더 많은 연구가 필요하리라 생각된다. Background : Epidemiological studies have shown correlation between low incidence of coronary heart disease and high consumption of fish products. It has been suggested that this may be due to the high content of polyunsaturated fatty acids of the n-3 fatty acid group in fish oil. In animal studies eicosapentaenoic and (EPA) inhibited attachment of monocytes to the arterial endothlium. Method : Adhesion assay was performed on the endothelial cells of the human umbilical vein with 25, 50, 100, 200 ㎛ EPA-treated U937 cells. The endothelial cells were activated with lipopoysaccharide (LPS). The adhesion assay was repeated with oxidized EPA. EPA was oxidized with CuSO4 and ascorbic acid. Result : Viability of U937 cells were not afected by concentrations up to 200 ㎛ of EPAand oxidized EPA. LPS treatment of endothelium notably increased the number of U937 cells attached to endothelial cells on the adhesion assay. However, treatment of EPA, native or oxidized, to U937 cells did not afect the number of U937 cells attached to LPS activated endothelial cells. Conclusion : EPA treatment, native or oxidized, of U937 cells did not affect U937 cell-endothlial cell adhesion. This suggests that inhibition of monocyte-endothlial cells ◎attachment by EPA is not due to the effects of EPS on monocytes.(Korean Circulatin J 1998;28(4):606-610)

Comparison of Physical Examination Test Validity for Determining Clinical Decision Making of Lumbar Stabilization Exercise

Eui-Ryong Kim(Eui-Ryong Kim) 한국임상움직임치료학회 2022 한국임상움직임치료학회지 Vol.2 No.1

Background: As a high false positive rate of Imaging based on patholo-anatomical models, it is difficult to classify and diagnose patients and with asymptomatic with only diagnoses by imaging. To provide more accurate diagnoses, subjective information based on the patterns of pain should be obtained physical tests should be conducted together with diagnoses by imaging. However, there is no study to examine the validity of physical tests. Objectives: The purpose of this study was to investigate the sensitivity, specificity, prediction rates, and likelihood ratios for those who had been diagnosed with the herniation of intervertebral discs or degenerative vertebral diseases and those who had not been diagnosed with these diseases through radiology. Methods: A total of 211 subjects were recruited in this study, with 137 chronic low back pain group (58 males, 79 females) receiving a medical diagnosis and 74 healthy control group (34 males, 40 females) not receiving a medical diagnosis. Physical examination tests in this study were rated using a positive and negative categorical scale. Then, the sensitivity, specificity, positive and negative predictive values, and positive and negative likelihood ratios (LR+, LR-) of the physical examination and medical diagnosis between chronic LBP and control group also were determined. Results Active movement test is high sensitivity, specificity, positive and negative predictive, positive and negative likelihood ratio than passive movement test. As increased age, the sensitivity was higher in passive movement test. Conclusions: The prone instability test (PIT), the active straight leg raise (ASLR), and the active hip abduction test (AHAT) are considered to be useful in diagnostic tests of chronic low back pain patients in clinics, and in particular, these tests are more sensitive to elderly persons aged 60 years or more.

Association of β-Lactoglobulin Variants with Milk Yield and Composition in Dairy Cattle

Eui Ryong Chung,Ku Young Chung 한국축산식품학회 2006 한국축산식품학회지 Vol.26 No.1

Association Analysis between SNP Marker in Neuopeptide Y (NPY) Gene and Carcass and Meat Quality Traits in Korean Cattle

Eui Ryong Chung,Sung Chul Shin,Jae Pil Heo 한국축산식품학회 2011 한국축산식품학회지 Vol.31 No.4

Biological or physiological genes that regulate metabolism and energy partitioning have the potential to influence economically important traits such as carcass and meat quality traits in beef cattle. The neuropeptide Y (NPY) functions as a central appetite stimulator and plays a major role in feed intake and energy-balance control. Therefore, the NPY gene is an excellent biological and physiological candidate gene for body weight, feeding, fatness or growth related traits in beef cattle. The objective of this study was to identify single nucleotide polymorphisms (SNPs) in the NPY gene and to evaluate the association of NPY SNP markers with carcass and meat quality traits in Korean cattle. The genomic region (711 bp) including intron 2 of NPY gene was amplified and sequenced, and five SNPs, g.4389 Del(C), g.4371Del(C), g.4271T>C, g.1899A>G and g.1517A>C, were identified. The PCR-RFLP method was then developed to genotype the individuals examined. The g.4271T>C SNP was significantly associated with M. Longissimus dori area (LDA) value (p

Intensification of the education of public health, hygiene, and martial arts during the Japanese colonial period (1937–1945)

Eui-Ryong Hwang,Tae-Young Kim 한국운동재활학회 2018 JER Vol.14 No.2

RESEARCH : Novel SNP in the coding region of the FTO gene is associated with marbling score in Hanwoo (Korean cattle)

( Eui Ryong Chung ) 한국동물자원과학회(구 한국축산학회) 2014 한국축산학회지 Vol.56 No.27

The fat mass and obesity associated (FTO) gene plays an important role in the regulation of energy homeostasis, fat deposition and obesity. For this reason, the FTO gene is a physiological and functional candidate gene for carcass and meat quality traits in beef cattle. The objectives of this study were to identify SNPs in the exonic regions of FTO gene and to evaluate the association of these SNPs with carcass traits in Hanwoo (Korean cattle). In this study, we newly identified two exonic SNPs in Hanwoo population. The g.125550A > T SNP was located in exon 3 and the g.175675C > T SNP was located in exon 6. Genotyping of the two SNP markers was carried out using PCR-RFLP analysis in Hanwoo steers to evaluate their association with carcass traits. As a result, g.125550A > T SNP genotype was significantly associated with effects on marbling score. Animals with the AA and TT homozygous genotypes had a significantly higher marbling score (p < 0.001) than those with AT heterozygous genotype, and this was significant after Bonferroni correction of the significance threshold (p = 0.003). Dominance effect was also observed for the marbling score (P < 0.05) with higher marbling score of homozygous animals. However, no significant associations with meat quality traits were observed for the g.175675C > T SNP. Our results suggest that the exonic SNP g.125550A > T in the FTO gene may be used as a DNA marker for the selection of Hanwoo with higher marbling.

Identification of Korean Native Pork Using Breed-Specific DNA Marker of KIT Gene

Eui Ryong Chung,Ku Young Chung 한국축산식품학회 2010 한국축산식품학회지 Vol.30 No.3

Accurate methods for the identification of closely related species or breeds in raw and processed meats must be developed in order to protect both consumers and producers from mislabeling and fraud. This paper describes the development of DNA markers for the discrimination and improvement of Korean native pig (KNP) meat. The KIT gene is related to pig coat color and is often used as a candidate marker. A 538 bp fragment comprising intron 19 of the pig KIT gene was amplified by PCR using specific primers, after which the PCR amplicons of a number of meat samples from KNP and three major improved breeds (Landrace, Duroc and Yorkshire) were sequenced in order to find a nucleotide region suitable for PCR-RFLP analysis. Sequence data showed the presence of two nucleotide substitutions, g.276G>A and g.295A>C, between KNP and the improved pig breeds. Digestion of KIT amplicons with AccII enzyme generated characteristic PCR-RFLP profiles that allowed discrimination between meats from KNP and improved pig. KNP showed three visible DNA bands of 264/249, 199, and 75 bp, whereas DNA bands of 249, 199, and 90 bp were detected in the three improved pig breeds. Therefore, the 75 bp DNA fragment was specific only to KNP, whereas the 90 bp DNA fragment was specific to the improved breeds. The breed-specific DNA markers reported here that target the KIT gene could be useful for the identification of KNP meat from improved pig meats, thus contributing to the prevention of falsified breed labeling.

Ultrasound Characteristics of The Abdominal Muscle Thickness during Prone Hip Extension Test in Subjects with and without Lower Back Pain

Eui-Ryong Kim,Byeong-Jin Kim,Won-Nyeong Cho,Min-Joo Ko 한국임상움직임치료학회 2023 한국임상움직임치료학회지 Vol.3 No.2

Background Deep abdominal muscles have a stabilizing role in the lumbo-pelvic region both increasing intra-abdominal pressure and lateral tension on thoracolumbar fascia. Prone hip extension (PHE) is commonly used as a reliable self-perturbation task to test lumbopelvic stability. To our knowledge, no study has directly evaluated abdominal muscle thickness with and without lower back pain (LBP) using ultrasound measurements during the PHE test. Objectives The purpose of the present study is to investigate the changes in the thickness of the trans-verse abdominis (TrA), internal oblique (IO), and external oblique (EO) during PHE using USI in subjects with and without LBP. Methods Seventy-three subjects (37 with LBP, 36 without LBP) were allocated. Three repeated ultra-sound imaging (USI) measurements were recorded in the prone resting position and during the PHE test in a day. Ultrasonography was used to determine the task-related changes in the thickness of abdominal muscles at resting and during the PHE test. Results The muscle thickness of the TrA and IO in the LBP group was significantly lower compared to without the LBP group during the PHE test (p < 0.05). While, the muscle thickness of the EO in the LBP group was significantly greater compared to without the LBP group during the PHE test (p < 0.05). Conclusions Our findings indicate that Individuals with LBP was lower muscle thickness of TrA and IO and greater muscle thickness of EO compared to the without LBP group. The findings of this study may be useful data for comparing the effects before and after exercise on muscle thickness of TrA, IO, and EO in LBP and planning treatment.

Association between SNP Marker of Uncoupling Protein 3 Gene and Meat Yield and Marbling Score Traits in Korean Cattle

Eui Ryong Chung,Sung Chul Shin,Jae Pil Heo 한국축산식품학회 2011 한국축산식품학회지 Vol.31 No.4

It is well established that uncoupling protein 3 (UCP3) is expressed largely in skeletal muscle, white adipose tissue and brown adipose tissue and has been suggested to play important roles in regulating energy expenditure, body weight, thermoregulation as well as fatty acid metabolism and obesity. Therefore, the UCP3 gene was selected as a candidate gene for carcass and meat quality traits in Korean cattle. The objective of this study was to identify single nucleotide polymorphisms (SNPs) in the UCP3 gene and to evaluate the association of UCP3 SNP markers with carcass and meat quality traits in Korean cattle. The five exons in the UCP3 gene were sequenced, and ten SNPs were identified. The PCR-SSCP method was then developed to genotype the individuals examined. The g.3076A>G genotype was significantly associated with marbling score (MS) of Korean cattle. Animals with the AA genotype had a higher MS than those with the AG and GG genotypes. No significant associations of the SNP g.3076A>G were observed for any traits. In conclusion, although SNP g.3076A>G, which showed an association with MS, does not cause amino acid changes, this SNP may be used as a DNA marker to select animals that have higher intramuscular fat content.