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이경림,Md. Fakruzzaman,Erdan Wang,김성수,하아나,민찬식,공일근 경상대학교 농업생명과학연구원 2014 농업생명과학연구 Vol.48 No.3
Matrix Metalloproteinases (both MMP2 and -9) play a pivotal role of the embryos hatchingand implantation. Therefore, the objective of this study was carried out to investigate theinfluence of MMP2 and MMP9 on embryo development potential and subsequent effect atmolecular level. There was no significant difference of cleavage rate among the groups. Thedevelopment competence of blastocyst was significantly higher (P<0.05) in MMP9 treatment(39.81±16.61) than that to the combined treatment of MMP2 and –9 (23.68±0.27), but there wasno significant difference among the control vs. MMP2 vs. MMP9 (35.05±2.74 vs. 32.71±6.18vs. 39.81±16.61, respectively). On the other hand, the hatching rate of blastocysts wassignificantly lower (P<0.05) in combined group of MMP2 and –9 (12.55±0.09) (Table1). Theexpression level of MMP2 and MMP9 was significantly lower (P<0.05) in the entire treatmentgroups than that in the control group. But the expression of MMP9 was significantly higher(P<0.05) when compared in the entire treatment groups. The relative expression embryonicdevelopmental gene, IFNt expression level significantly lower (P < 0.05) in the MMP9 embryos. The placenta establishment genes, PLAC8 and SSLP1, expression were significantly higher (P <0.05) in the MMP2 embryos compared to other groups. Transcription regulation gene,HNRNPA2B1, was higher (P < 0.05) in the combined group of MMP2+MMP9 than that in theother groups. In conclusion, our results suggest that MMPs to culture medium improves theblastocyst development rate and further impact on target gene expression analysis.
이준기,박시현,김복희,Erdan Gu,조훈,Ian Watson,Martin Dawson 한국물리학회 2006 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.49 No.1
We report the processing of InGaN/GaN epifilms on GaN-silicon substrates. High-quality In- GaN/GaN multi-quantum wells (MQWs) were grown on GaN-silicon substrates, and their membranes were successfully fabricated using a selective wet etching of silicon followed by a dry etching of the AlGaN buffer layer. With atomic force microscope (AFM) measurements and photoluminescence (PL) measurements, we investigated the physical and the optical properties of the InGaN/GaN MQWs membranes. On the InGaN/GaN MQW membranes, dielectric distributed Bragg reflectors (DBRs) were successfully deposited, which give, new possibilities for use in GaN microcavity and surface-emitting laser fabrication.
Development of New Vitrification Method for Preimplantation Mouse Embryo
A-Na Ha,Md. Fakruzzaman,Kyeong-Lim Lee,Erdan Wang,Jae-Ik Lee,Chan-Sik Min,Il-Keun Kong 韓國受精卵移植學會 2013 한국동물생명공학회지 Vol.28 No.2
The purpose of this study was attempted to new methods in mammalian embryos vitrification. This method was affected to increase of the embryo vitrification efficiency and it would be applied to the field of embryo transfer to recipient by modified loading method of embryo into 0.25 ml plastic straw. The frozen mouse embryos were carried out warmed from two different cell stages (8-cell and blastocyst, respectively) by attachment of an embryo in the vitrification straw (aV) method. All groups were cultured in M-16 medium to determine the development and survivability for 24 h, respectively. Results shown that, the survivability of two different groups were significantly different (94.8% vs. 70.9%). Total cell number was not significantly different the non-frozen blastocyst (99.7 ± 12.4) compared to the post-thaw blastocyst (94.8 ± 15.1). From the 8-cell embryo, total cell number of frozen blastocysts were significantly lower than others groups (74.7 ± 14.6, p<0.05). In the case of cell death analysis, the blastocysts from non-frozen and frozen-thawed 8-cell group were not different (0.0 ± 0.0 vs. 1.9 ± 3.1, p>0.05). However, the apoptotic nuclei of blastocyst were significantly observed the frozen-thawed group (5.4 ± 4.4) compared to non-frozen group (p<0.05). Therefore, this new method of embryos using in-straw dilution and direct transfer into other species would be more simple procedure of embryo transfer rather than step-wise dilution method and cryopreservation vessels, so we can be applied in animal as well as human embryo cryopreservation in further.
Development of New Vitrification Method for Preimplantation Mouse Embryo
Ha, A-Na,Fakruzzaman, Md.,Lee, Kyeong-Lim,Wang, Erdan,Lee, Jae-Ik,Min, Chan-Sik,Kong, Il-Keun The Korean Society of Embryo Transfer 2013 한국동물생명공학회지 Vol.28 No.2
The purpose of this study was attempted to new methods in mammalian embryos vitrification. This method was affected to increase of the embryo vitrification efficiency and it would be applied to the field of embryo transfer to recipient by modified loading method of embryo into 0.25 ml plastic straw. The frozen mouse embryos were carried out warmed from two different cell stages (8-cell and blastocyst, respectively) by attachment of an embryo in the vitrification straw (aV) method. All groups were cultured in M-16 medium to determine the development and survivability for 24 h, respectively. Results shown that, the survivability of two different groups were significantly different (94.8% vs. 70.9%). Total cell number was not significantly different the non-frozen blastocyst ($99.7{\pm}12.4$) compared to the post-thaw blastocyst ($94.8{\pm}15.1$). From the 8-cell embryo, total cell number of frozen blastocysts were significantly lower than others groups ($74.7{\pm}14.6$, p<0.05). In the case of cell death analysis, the blastocysts from non-frozen and frozen-thawed 8-cell group were not different ($0.0{\pm}0.0$ vs. $1.9{\pm}3.1$, p>0.05). However, the apoptotic nuclei of blastocyst were significantly observed the frozen-thawed group ($5.4{\pm}4.4$) compared to non-frozen group (p<0.05). Therefore, this new method of embryos using in-straw dilution and direct transfer into other species would be more simple procedure of embryo transfer rather than step-wise dilution method and cryopreservation vessels, so we can be applied in animal as well as human embryo cryopreservation in further.