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A Detection Kit for Aeromonas hydrophila Using Antibody Sensitized Latex
SHIN, ENJOO,LEE, SOONDEUK,LEE, KYUNGWON,LEE, YEONHEE 한국미생물 · 생명공학회 2000 Journal of microbiology and biotechnology Vol.10 No.5
Aeromonas hydrophila is a pathogen to fish as well as human. It is a food-borne disease, and causes severe mortality in fish, and sometimes severe septicemia in human. In this study, a rapid detection method using latex agglutination has been developed for A. hydrophila. Polyclonal antibodies were raised against membrane and whole cells of three isolates from rainbow trout. Among these, latex particles coated with antibodies raised against whole cells of isolate No. 2 showed the best sensitivity. With latex particles coated with this antibody, we could detect 5×10 exp (4) CFU of A. hydrophila in 5min. The cross-reactivity with bacteria constituting the normal intestinal microflora and other pathogens for rainbow trout was insignificant. This latex agglutination assay method produced positive reaction with all clinical isolates of A. hydrophila which were identified by species-specific PCR for 16S rRNA in A. hydrophila.
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증강현실을 이용한 사회적 상호작용시 정보처리에 관한 연구: 실세계 상호작용과의 비교를 중심으로
유재환(Jaehwan You),허지웅(Jiwoong Heo),김은주(Enjoo Kim),김광욱(Kwanguk Kim) 한국정보과학회 2021 정보과학회논문지 Vol.48 No.3
증강현실 기술과 이를 활용하는 과제 수행에 관한 연구는 최근 활발히 이루어지고 있다. 그러나 학습과 인지발달에 핵심이 되는 사회적 상호작용시 증강현실을 활용하는 연구는 매우 제한적이다. 본 연구에서는 사회적 상호작용 가운데, 두 사람의 주의가 공유된 공동주의 상황에서 증강현실 기기를 사용하는 피험자의 정보처리 능력의 변화에 대해 연구하였다. 35명을 대상으로 증강현실을 이용한 상호작용과 실제 상호작용을 비교하였으며, 실험조건은 피험자가 공동주의를 주도하는 조건과 따라가는 조건을 포함하였다. 측정 변인은 목표자극, 비목표자극, 피험자의 머리움직임을 포함하였다. 연구결과 증강현실을 활용한 공동주의 상호작용시 목표자극에 대한 정보처리에서는 유의미한 차이가 없었으나, 비목표자극에 대한 정보처리와 전체 머리움직임에서 일부 차이가 있음을 확인하였다. 이러한 연구 결과는 사람 간의 사회적 상호작용시 증강현실 기기가 유용함을 보여주고, 동시에 증강현실 기기에서 개선되어야 할 점이 있음을 제안한다. Research on augmented reality (AR) has focused on the development of technologies and its task performances. However, the effect of AR technologies on social interaction is yet to be rigorously examined. Social interaction is one of the key components of human learning and cognitive developments. In this study, we compared initiating and responding joint attention with the social information processing task, and each participant conducted both AR and real-world conditions. Thirty-three participants were enrolled in the current study, and dependent measures included accuracies of target, non-target, and novel pictures, and total head-movements. The results suggested that there were no significant differences in information processing of target and novel pictures, but we found that accuracies of non-target pictures and total head-movements were significantly different between AR and real-world conditions. These results suggested that AR devices can be used for social information processing tasks, but they need improvements, which are discussed in the current study.
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잔류유분 함유 반출처리토 재활용을 위한 호밀 식재 식물상 토성회복 가능성
박지은,배범한,주완호,배세달,배은주,Park, Jieun,Bae, Bumhan,Joo, Wanho,Bae, Seidal,Bae, Enjoo 한국지하수토양환경학회 2014 지하수토양환경 Vol.19 No.3
The amount of TPH contaminated soil treated at off-site remediation facilities is ever increasing. For the recycle of the treated-soil on farmlands, it is necessary to restore biological and physico-chemical soil characteristics and to remove residual TPH in the soil by an economic polishing treatment method such as phytoremediation. In this study, a series of experiments was performed to select suitable plant species and to devise a proper planting method for the phyto-restoration of TPH-treated soil. Rye (Secale cereale) was selected as test species through a germination test, among 5 other plants. Five 7-day-old rye seedlings were planted in a plastic pot, 20 cm in height and 15 cm in diameter. The pot was filled with TPH-treated soil (residual TPH of 1,118 mg/kg) up to 15 cm, and upper 5 cm was filled with horticulture soil to prevent TPH toxic effects and to act as root growth zone. The planted pot was cultivated in a greenhouse for 38 days along with the control that rye planted in a normal soil and the blank with no plants. After 38 days, the above-ground biomass of rye in the TPH-treated soil was 30.6% less than that in the control, however, the photosynthetic activity of the leaf remained equal on both treatments. Soil DHA (dehydrogenase activity) increased 186 times in the rye treatment compared to 10.8 times in the blank. The gross TPH removal (%) in the planted soil and the blank soil was 34.5% and 18.4%, respectively, resulting in 16.1% increase of net TPH removal. Promotion of microbial activity by root exudate, increase in soil permeability and air ventilation as well as direct uptake and degradation by planted rye may have contributed to the higher TPH removal rate. Therefore, planting rye on the TPH-treated soil with the root growth zone method showed both the potential of restoring biological soil properties and the possibility of residual TPH removal that may allow the recycle of the treated soil to farmlands.
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