In vitro Evaluation of Cytotoxic Activities of Essential Oil from Moringa oleifera Seeds on HeLa, HepG2, MCF-7, CACO-2 and L929 Cell Lines

Elsayed, Elsayed Ahmed,Sharaf-Eldin, Mahmoud A.,Wadaan, Mohammad Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.11

Moringa oleifera Lam. (Moringaceae) is widely consumed in tropical and subtropical regions for their valuable nutritional and medicinal characteristics. Recently, extensive research has been conducted on leaf extracts of M. oleifera to evaluate their potential cytotoxic effects. However, with the exception of antimicrobial and antioxidant activities, little information is present on the cytotoxic activity of the essential oil obtained from M. oleifera seeds. Therefore, the present investigation was designed to investigate the potential cytotoxic activity of seed essential oil obtained from M. oleifera on HeLa, HepG2, MCF-7, CACO-2 and L929 cell lines. The different cell lines were subjected to increasing oil concentrations ranging from 0.15 to 1 mg/mL for 24h, and the cytotoxicity was assessed using MTT assay. All treated cell lines showed a significant reduction in cell viability in response to the increasing oil concentration. Moreover, the reduction depended on the cell line as well as the oil concentration applied. Additionally, HeLa cells were the most affected cells followed by HepG2, MCF-7, L929 and CACO-2, where the percentages of cell toxicity recorded were 76.1, 65.1, 59.5, 57.0 and 49.7%, respectively. Furthermore, the $IC_{50}$ values obtained for MCF-7, HeLa and HepG2 cells were 226.1, 422.8 and $751.9{\mu}g/mL$, respectively. Conclusively, the present investigation provides preliminary results which suggest that seed essential oil from M. oleifera has potent cytotoxic activities against cancer cell lines.

Dynamics of Recursive Sequence of Order Two

Elsayed, Elsayed Mohammed Department of Mathematics 2010 Kyungpook mathematical journal Vol.50 No.4

In this paper we study some qualitative behavior of the solutions of the difference equation $x_{n+1}=ax_n=\frac{bx_n}{cx_n-dx_{n-1}}$, n=0,1,$\ldots$, where the initial conditions x-1, x0 are arbitrary real numbers and a, b, c, d are positive constants with $cx_0-dx_{-1}\neq0$.

Pseudasphondylia ishigakiensis, a new gall midge species (Diptera: Cecidomyiidae) from Japan developing in flower buds of Melicope triphylla (Rutaceae), with notes on its life history

Elsayed Ayman Khamis,Nemoto Shuichi,Tokuda Makoto 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.2

A gall midge species (Diptera: Cecidomyiidae) associated with flower buds of Melicope triphylla (Lam.) Merr. (Rutaceae) was found on Ishigaki Island, southwestern Okinawa Prefecture, Japan. Morphological examination and molecular analysis indicated that it is an undescribed species of the genus Pseudasphondylia Monzen (Cecidomyiidae: Cecidomyiinae: Asphondyliini). The species is herein described as Pseudasphondylia ishigakiensis Elsayed sp. n. and notes on its life history are reported. In addition, a molecular phylogenetic analysis based on a fragment of COI mitochondrial gene comparing the new species with other known Pseudasphondylia in Japan was conducted to confirm the species distinctness.

Simulation Study of a New Approach for Field Weakening Control of PMSM

Elsayed, Mohamed Taha,Mahgoub, Osama Ahmed,Zaid, Sherif Ahmed The Korean Institute of Power Electronics 2012 JOURNAL OF POWER ELECTRONICS Vol.12 No.1

In this paper, the different techniques for the field weakening, also known as constant power speed range (CPSR) operation, for permanent magnet synchronous motor (PMSM) will be introduced and analysed. Field weakening of PMSM, can be done using either vector control (VC) or conventional phase in advance (CPA). Implementation of these techniques depending on some features and constrains. Most of these features and constrains came from the motor parameters. One of these constrains is the motor inductance which determining whether the motor can be driven in the CPSR or not. A new approach for the field weakening will be discussed and to be verified to overcome this constrain. The new approach will be verified through both techniques VC and CPA.

Using nano-magnesium oxide/bentonite composite for cadmium removal from industrial wastewater

Elsayed A. Elkhatib,Mohamed L. Moharem,Ahmed F. Saad,Farida A. Attia 대한환경공학회 2023 Environmental Engineering Research Vol.28 No.2

Nowadays, the removal of toxic heavy metals from industrial wastewater is a long standing problem due to the industrial progress worldwide. This study mainly focused on the production of a novel nMgO-bentonite nanocomposite using sol–gel method to enhance Cd ions removal from industrial wastewater. The nMgO-bentonite nano-composite was characterized for structural morphology and presence of functional groups using SEM/EDS, XRD and FTIR analysis. Cadmium sorption equilibrium and kinetic data were well fitted to Langmuir and power function models respectively as confirmed by the highest R² (0.965), and the lowest SE (38<SUP>*</SUP>10<SUP>-6</SUP>) values among other studied models. The Langmuir removal capacity of the nanocomposite is 200 ㎎/g which is 60 times greater than that of bentonite. Increased Cd sorption on nano-composite with increased temperature from 287 to 307K and the thermodynamic results revealed that Cd adsorption process was endothermic and spontaneous. The data also showed highly repetitive application of nano-composite during six cycles of adsorption/ desorption experiment. The final results pointed out that nMgO-bentonite nano-composite has great adsorption affinity for Cd and could potentially employ as a cost-effective, ecofriendly and efficient sorbent for Cd removal from contaminated wastewater.

Molecular Marker Assisted for Recognition Drought Tolerant in Some of Bread Wheat Genotypes

ElSayed, Abdelaleim Ismail,Rafudeen, Muhammad Suhail 한국작물학회 2012 Journal of crop science and biotechnology Vol.15 No.1

To develop crop plants with enhanced tolerance of drought stress, a basic understanding of physiological, biochemical, and genetic networks is essential. Four bread wheat genotypes and one wheat line were evaluated for molecular indicators of drought tolerance using RAPD-PCR and protein profiling. The RAPD markers were used to determine the genetic differences between the five wheat genotypes and to determine the molecular markers associated with tolerance to drought. The present study found that RAPD analysis is a valuable diagnostic tool when different sets of RAPD primers were used to study the polymorphism at the molecular level. A total of 72 alleles were amplified with six random primers out of which 61% were monomorphic and 38% were polymorphic. Primer B8 amplified a 600 bp band in Sham-6 which is assumed to be a drought-tolerant genotype, while primer A-8 amplified a 550 bp band in genotypes Giza-168 and Sham-6. Genetically, the most similar genotypes were Sham-6 and Line-7 (93%) followed by Gemaza-9 and Giza-168 (92%) while the most dissimilar genotype was Sakha-93 (86%). Protein profiling revealed differences between the genotypes with a protein band presents at 130 KDa in the Sham-6, Gemaza-9, and Sakha-93 genotypes and absent in Line-7 and Giza-168. Proline content was highest in the drought-tolerant genotypes, Sham-6 and Sakha-93. Sucrose content in shoots was increased in tolerant plants (Sakha-93 and Sham-6), while there was a reduction in sucrose in the shoot tissues of the seedling stage of Gemaza-9, Line-7, and Giza-168. Overall, the accumulation of reducing sugars was lowest in all plants compared with sucrose content.

Resistin and Insulin Resistance: A Link Between Inflammation and Hepatocarcinogenesis

Elsayed, Engy Yousry,Mosalam, Nesreen Ahmed,Mohamed, Noha Refaat Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.16

Background: Hepatocellular carcinoma (HCC) is the sixth most common cancer and the third leading cause of cancer related death overall. The role of insulin resistance in the development of HCC associated with chronic HCV infection has not been established. Resistin is a polypeptide hormone belonging to the adipokine family which could contribute to tumorigenesis and angiogenesis. Our aim was to study serum resistin and insulin resistance as risk factors for HCC in HCV cirrhotic patients. Materials and Methods: This prospective case controlled study included 100 patients with HCV related liver cirrhosis and HCC, 100 patients with HCV related liver cirrhosis without HCC and 50 apparently healthy participants as controls. For all subjects, liver profile, serologic markers for viral hepatitis, lipid profile, alpha-fetoprotein level (AFP), homeostasis model assessment (HOMA) were examined along with resistin. Results: HCC patients had higher mean values of HOMA-IR and resistin than cirrhotic patients and the control subjects (p<0.01). HOMA and resistin were considered independent risk factors in development of HCC, those patients with resistin > 12 ng/ml and HOMA > 4 being 1.6 times more likely to have HCC. Conclusions: HOMA and serum resistin allow for early identification of patients with cirrhosiswho are at substantially increased risk of HCC. Recommendation: HOMA and serum resistin could represent novel markers to identify HCV cirrhotic patients at greater risk of development of HCC.

Design and In vitro Investigation of Grafted Chitosan as Chitosan Nanoparticles Containing Deflazacort Drug Coated by Calcium Starch Glycolate

Elsayed M. Abdel Bary,Ammar N. Harmal,Mona E. Ibrahim,Moustafa A. Gouda 한국섬유공학회 2020 Fibers and polymers Vol.21 No.12

Novel chitosan nanoparticles (SCN) were synthesized via reaction of chitosan with different concentration of 2-chloro-N-(3-methylisoxazol-5-yl) acetamide (5 %, 10 % and 15 %) at 70-80 oC for 7 h to give the grafted polymer, whichcondensed with cyclohexanone Mannich base 45 oC for 7 h to afford the corresponding (SCN). These were stirred withDeflazacort drug to achieve the Deflazacort loading (SCN), which, compressed with calcium starch glycolate (CaSgly) togive the corresponding tablets. The pH 2. 1 and concentration of SCN-10 %/CaSgly (1:4) was found to be importantparameters in controlling the release profiles.

Water resources in Egypt and the challenge for water in the twenty first century

Elsayed M. Abu El Ella 경희대학교 지구환경연구소 2001 국제세미나 Vol.2001 No.-

An Interactive Multi-Factor User Authentication Framework in Cloud Computing

Elsayed Mostafa,M.M. Hassan,Wael Said International Journal of Computer ScienceNetwork S 2023 International journal of computer science and netw Vol.23 No.8

Identity and access management in cloud computing is one of the leading significant issues that require various security countermeasures to preserve user privacy. An authentication mechanism is a leading solution to authenticate and verify the identities of cloud users while accessing cloud applications. Building a secured and flexible authentication mechanism in a cloud computing platform is challenging. Authentication techniques can be combined with other security techniques such as intrusion detection systems to maintain a verifiable layer of security. In this paper, we provide an interactive, flexible, and reliable multi-factor authentication mechanisms that are primarily based on a proposed Authentication Method Selector (AMS) technique. The basic idea of AMS is to rely on the user's previous authentication information and user behavior which can be embedded with additional authentication methods according to the organization's requirements. In AMS, the administrator has the ability to add the appropriate authentication method based on the requirements of the organization. Based on these requirements, the administrator will activate and initialize the authentication method that has been added to the authentication pool. An intrusion detection component has been added to apply the users' location and users' default web browser feature. The AMS and intrusion detection components provide a security enhancement to increase the accuracy and efficiency of cloud user identity verification.