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Approximating vibronic spectroscopy with imperfect quantum optics
Clements, William R,Renema, Jelmer J,Eckstein, Andreas,Valido, Antonio A,Lita, Adriana,Gerrits, Thomas,Nam, Sae Woo,Kolthammer, W Steven,Huh, Joonsuk,Walmsley, Ian A IOP 2018 Journal of physics B, Atomic, molecular, and optic Vol.51 No.24
<P>We study the impact of experimental imperfections on a recently proposed protocol for performing quantum simulations of vibronic spectroscopy. Specifically, we propose a method for quantifying the impact of these imperfections, optimizing an experiment to account for them, and benchmarking the results against a classical simulation method. We illustrate our findings using a proof of principle experimental simulation of part of the vibronic spectrum of tropolone. Our findings will inform the design of future experiments aiming to simulate the spectra of large molecules beyond the reach of current classical computers.</P>
Wajima Safi,Andreas Kuehnl,Andreas Nussler,Hans-Henning Eckstein,Jaroslav Pelisek 생화학분자생물학회 2016 Experimental and molecular medicine Vol.48 No.-
The aim of this study was to determine the optimal culturing media for human CD14+ monocytes and to evaluate whether these cells are capable of differentiating into vascular endothelial cells. Human monocytes isolated from peripheral blood were cultured for 1, 3, 7, 10 or 14 days in different media containing either 10% fetal bovine serum (FBS), 10% autologous donor serum (Auto), 10% FBS with interleukin-3 and macrophage colony stimulating factor (FBS-WF) or 10% Auto and the same growth factors (AU-WF). The cells were differentiated using endothelial cell conditioning medium (EC). Viability was measured using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, and the cells were characterized by histology, immunohistochemistry and western blot analysis. Monocytes treated with Auto, FBS-WF or AU-WF medium generated a significant higher yield of vital cells after 7 days in culture compared with FBS-only medium (mean difference (MD) = 0.318, P = 0.01; MD = 1.83, P = 0.04; or MD = 0.271, P = 0.01 and MD = 0.318, P = 0.102). All tested media led to the differentiation of monocytes into macrophages, identified by CD68, especially in the FBS-WF medium (MD = +18.3%; P = 0.04). Differentiation into ECs caused a significant decrease in cell viability in all media. Endothelial cell markers, including CD31, CD144, VEGF, VEGF-R2 and CD34, could not be detected. Autologous serum significantly increases the yield of monocytederived cells with a higher effectiveness than commonly used FBS-only serum. There is no further benefit in culturing monocytes longer than 7 days. The cultivation of monocytes in the tested media leads preferentially to differentiation into macrophages. Differentiation into endothelial cells did not take place.
Kosmas I. Paraskevas,Dimitri P. Mikhailidis,Hediyeh Baradaran,Alun H. Davies,Hans-Henning Eckstein,Gianluca Faggioli,Jose Fernandes e Fernandes,Ajay Gupta,Mateja K. Jezovnik,Stavros K. Kakkos,Niki Kat 대한뇌졸중학회 2021 Journal of stroke Vol.23 No.2
The optimal management of patients with asymptomatic carotid stenosis (ACS) is the subject of extensive debate. According to the 2017 European Society for Vascular Surgery guidelines, carotid endarterectomy should (Class IIa; Level of Evidence: B) or carotid artery stenting may be considered (Class IIb; Level of Evidence: B) in the presence of one or more clinical/imaging characteristics that may be associated with an increased risk of late ipsilateral stroke (e.g., silent embolic infarcts on brain computed tomography/magnetic resonance imaging, progression in the severity of ACS, a history of contralateral transient ischemic attack/stroke, microemboli detection on transcranial Doppler, etc.), provided documented perioperative stroke/death rates are <3% and the patient’s life expectancy is >5 years. Besides these clinical/imaging characteristics, there are additional individual, ethnic/racial or social factors that should probably be evaluated in the decision process regarding the optimal management of these patients, such as individual patient needs/patient choice, patient compliance with best medical treatment, patient sex, culture, race/ethnicity, age and comorbidities, as well as improvements in imaging/operative techniques/outcomes. The present multispecialty position paper will present the rationale why the management of patients with ACS may need to be individualized.
Fabian Lohoefer,Christian Reeps,Christina Lipp,Martina Rudelius,Felix Haertl,Edouard Matevossian,Alma Zernecke,Hans-Henning Eckstein,Jaroslav Pelisek 생화학분자생물학회 2014 Experimental and molecular medicine Vol.46 No.-
Cysteine and aspartic proteases possess high elastolytic activity and might contribute to the degradation of the abdominal aortic aneurysm (AAA) wall. The aim of this study was to analyze, in detail, the proteases (cathepsins B, D, K, L and S, and inhibitor cystatin C) found in human AAA and healthy aortic tissue samples. The vessel walls from AAA patients (n¼36) and nonaneurysmal aortae (n¼10) were retrieved using conventional surgical repair and autopsy methods. Serum samples from the same AAA patients and 10 healthy volunteers were also collected. Quantitative expression analyses were performed at the mRNA level using real-time reverse transcriptase-PCR (RT–PCR). Furthermore, analyses at the protein level included western blot and immunoprecipitation analyses. Cellular sources of cysteine/aspartic proteases and cystatin C were identified by immunohistochemistry (IHC). All cysteine/aspartic proteases and cystatin C were detected in the AAA and control samples.Using quantitative RT–PCR, a significant increase in expression was observed for cathepsins B (P¼0.021) and L (P¼0.018),compared with the controls. Cathepsin B and cystatin C were also detected in the serum of AAA patients. Using IHC, smooth muscle cells (SMCs) and macrophages were positive for all of the tested cathepsins, as well as cystatin C; in addition, the lymphocytes were mainly positive for cathepsin B, followed by cathepsins D and S. All cysteine/aspartic proteases analyzed in our study were detected in the AAA and healthy aorta. The highest expression was found in macrophages and SMCs. Consequently, cysteine/aspartic proteases might play a substantial role in AAA.