Electrochemical and Spectroscopic Study on the Corrosion of Ti–5Al and Ti–5Al–5Cu in Chloride Solutions

El‑Sayed M. Sherif,Fahamsyah H. Latief,Hany S. Abdo,Nabeel H. Alharthi 대한금속·재료학회 2019 METALS AND MATERIALS International Vol.25 No.6

In this study, manufacturing of Ti–5Al and Ti–5Al–5Cu alloys were accomplished employing mechanical alloying technique. The corrosion resistance in 3.5 wt% NaCl solution of Ti–5Al and Ti–5Al–5Cu alloys was investigated using cyclicpolarization (CPP), electrochemical impedance spectroscopy (EIS) and chronoamperometric current–time measurements. The corroded surfaces of Ti–5Al and Ti–5Al–5Cu were examined by the use of a scanning electron microscopy and energydispersive X-ray spectroscopy. It is found that Ti–5Al suffers both uniform and pitting corrosion, particularly with prolongingthe time of exposure period in the chloride solution. While, the addition of Cu, Ti–5Al–5Cu alloy, increases the intensity ofuniform corrosion and decreases the probability of pitting attack. Prolonging the immersion time to 48 h before measurementdecreases the corrosion of Ti–5Al alloy, while increases the corrosion of Ti–5Al–5Cu.

Alleviation of salt stress in  Triticum aestivum by biopriming with  Phanerochaete chrysosporium

Hanan El-Sayed Dief,El-Sayed A. Hashem,Salwa Fawzan,Ashraf S. A. El-Sayed 한국작물학회 2021 Journal of crop science and biotechnology Vol.24 No.1

Wheat is one of the most important cereals, vital basic crop in Egypt, covering approximately 32.6% of the total winter land. However, sensitivity to abiotic stress especially salinity is one the major current hurdles that reduce the global yield of this crop. Plant biopriming with fungi is the recent technology that might alleviate the salt stress eff ect. Thus, the objective of this work was to examine the eff ect of Triticum aestivum grains biopriming with Phanerochaete chrysosporium on the response of wheat to salt stress. From the physiological and biochemical parameters, biopriming of wheat grains with P. chrysosporium significantly alleviates the salt stress and markedly increases the growth parameters, photosynthetic pigments and osmolytes (soluble sugars, soluble protein and proline) contents. In addition, it alleviates the oxidative damage, as indicated by the lower accumulation of malondealdehyde and increasing the activity of antioxidant enzymes; superoxide dismutase, catalase, and ascorbate peroxidase in wheat seedlings. Results indicate the potential of using P. chrysosporium biopriming for reducing the deteriorating eff ects of salinity.

Adsorption of lisinopril and chlorpheniramine from aqueous solution on dehydrated and activated carbons

El-Said I. El-Shafey,Haider A. J. Al-Lawati,Wafa S. H. Al-Saidi 한국탄소학회 2016 Carbon Letters Vol.19 No.-

Date palm leaflets were used as a precursor to prepare dehydrated carbon (DC) via phosphoric acid treatment at 150°C. DC, acidified with H3PO4, was converted to activated carbon (AC) at 500°C under a nitrogen atmosphere. DC shows very low surface area (6.1 m2/g) while AC possesses very high surface area (829 m2/g). The removal of lisinopril (LIS) and chlorpheniramine (CP) from an aqueous solution was tested at different pH, contact time, concentration, and temperature on both carbons. The optimal initial pH for LIS removal was 4.0 and 5.0 for DC and AC, respectively. However, for CP, initial pH 9.0 showed maximum adsorption on both carbons. Adsorption kinetics showed faster removal on AC than DC with adsorption data closely following the pseudo second order kinetic model. Adsorption increases with temperature (25°C–45°C) and activation energy (Ea) is in a range of 19–25 kJ mol/L. Equilibrium studies show higher adsorption on AC than DC. Thermodynamic parameters show that drug removal is endothermic and spontaneous with physical adsorption dominating the adsorption process. Column adsorption data show good fitting to the Thomas model. Despite its very low surface area, DC shows ~70% of AC drug adsorption capacity in addition of being inexpensive and easily prepared.

SEMIGROUPS AND HIGHER ORDER GENERATORS

El-Sayed, A.M.A. Department of Mathematics 1992 Kyungpook mathematical journal Vol.32 No.1

Characterization of Homocysteine γ-Lyase from Submerged and Solid Cultures of Aspergillus fumigatus ASH (JX006238)

( El Sayed Ashraf S ),( Salwa A Khalaf ),( Hani A Aziz ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.4

Among 25 isolates, Aspergillus fumigatus ASH (JX006238) was identified as a potent producer of homocysteine γ- lyase. The nutritional requirements to maximize the enzyme yield were optimized under submerged (SF) and solid-state fermentation (SSF) conditions, resulting in a 5.2- and 2.3-fold increase, respectively, after the last purification step. The enzyme exhibited a single homogenous band of 50 kDa on SDS-PAGE, along with an optimum pH of 7.8 and pH stability range of 6.5 to 7.8. It also showed a pI of 5.0, as detected by pH precipitation with no glycosyl residues. The highest enzyme activity was obtained at 37-40℃, with a Tm value of 70.1oC. The enzyme showed clear catalytic and thermal stability below 40℃, with T1/2 values of 18.1, 9.9, 5.9, 3.3, and 1.9 h at 30℃, 35℃, 40℃, 50℃, and 60℃, respectively. Additionally, the enzyme Kr values were 0.002, 0.054, 0.097, 0.184, and 0.341 S-1 at 30oC, 35oC, 40oC, 50℃, and 60℃, respectively. The enzyme displayed a strong affinity to homocysteine, followed by methionine and cysteine when compared with non-S amino acids, confirming its potency against homocysteinuriarelated diseases, and as an anti-cardiovascular agent and a specific biosensor for homocysteinuria. The enzyme showed its maximum affinity for homocysteine (Km 2.46 mM, Kcat 1.39 × 10-3 s-1), methionine (Km 4.1 mM, Kcat 0.97 × 10-3 s-1), and cysteine (Km 4.9 m M, Kcat 0.77 × 10-3 s-1). The enzyme was also strongly inhibited by hydroxylamine and DDT, confirming its pyridoxal 5`-phosphate (PLP) identity, yet not inhibited by EDTA. In vivo, using Swiss Albino mice, the enzyme showed no detectable negative effects on platelet aggregation, the RBC number, aspartate aminotransferase, alanine aminotransferase, or creatinine titer when compared with negative controls.

Fabrication of gold nanoparticle modified ITO substrate to detect beta-amyloid using surface-enhanced Raman scattering.

El-Said, Waleed Ahmed,Kim, Tae-Hyung,Yea, Cheol-Heon,Kim, Hyuncheol,Choi, Jeong-Woo American Scientific Publishers 2011 Journal of nanoscience and nanotechnology Vol.11 No.1

<P>Alzheimer's disease is a progressive neurodegenerative disorder that is characterized by the deposition of beta-amyloid (Abeta) peptide and the formation of neurofibrillary tangles in neurons. The Abeta peptide is a key molecule in the pathogenesis of Alzheimer's disease and an important marker for early diagnosis. Surface-enhanced Raman scattering (SERS) has recently been attracting keen interest in various fields such as for biosensors or immunoassays. In this study, gold nanoparticles (Au NPs) were electrochemically deposited on an indium tin oxide (ITO) substrate at different heights. Abeta antibodies were immobilized on the Au-NP-coated ITO substrate, after which the interactions between the antigen and the antibody were determined via SERS spectroscopy. The SERS responses were strongest at the Au NP array height of 91 nm, with a good linear relationship that corresponded to the change in the concentration of the antigen. This Au-NP-array-mediated SERS can be applied with a highly sensitive immunodetection biosensor.</P>

Immune Defense of Rats Immunized with Fennel Honey, Propolis, and Bee Venom Against Induced Staphylococcal Infection

Sayed, S.M.,El-Ella, Ghada A. Abou,Wahba, Nahed M.,Nisr, Neveen A. El,Raddad, Khaled,Rahman, M.F. Abd El,Hafeez, M.M. Abd El,Aamer, Ahmed Abd El Fattah The Korean Society of Food Science and Nutrition 2009 Journal of medicinal food Vol.12 No.3

The objective of this work was to evaluate the potency of bee product-immunized rats to overcome an induced Staphylococcus aureus infection. Forty rats were divided to eight groups: T1, T3, and T5 received, respectively, fennel honey, ethanol, and aqueous propolis extracts orally, and T2, T4, and T6 were administered the respective materials intraperitoneally; T7 received bee venom by the bee sting technique; and T8 was the control group. All groups were challenged by a bovine clinical mastitis isolate of S. aureus. Each rat received 2mL of broth inoculated with $1{\times}10^5$ colony-forming units/mL intraperitoneally. Two weeks post-induced infection all rats were sacrificed and eviscerated for postmortem inspection and histopathological study. Three rats from T8 and one rat from T7 died before sacrifice. Another two rats, one each in T4 and T5, had morbidity manifestations. The remaining experimental animals showed apparently healthy conditions until time of sacrifice. Postmortem inspection revealed that all T8 rats showed different degrees of skeletal muscle and internal organ paleness with scattered focal pus nodules mainly on lungs and livers. All rats of the treated groups showed normal postmortem features except three rats. A dead rat in group T7 showed focal pus nodules on the lung surface only, whereas the affected two rats in groups T4 and T5 appeared normal except with some pus nodules, but much smaller than in the control, scattered on the hepatic surface and mesentery. Histopathological studies revealed that T8 rats had typical suppurative bronchopneumonia and or severe degenerative and necrobiotic changes in hepatic tissues. Three affected rats of the treated groups showed slight bronchopneumonia or degenerative hepatic changes only. The other animals of the treated groups showed completely normal parenchymatous organs with stimulated lymphatic tissues. It was concluded that all tested previously bee product-immunized rats could significantly challenge the induced S. aureus infection (P < .01). The effects were more pronounced in rats that had received fennel honey solution.

A MIXED PROBLEM FOR A SECOND ORDER STURM-LIOUVILLE EQUATION

El-Sayed, A.M.A. Department of Mathematics 1992 Kyungpook mathematical journal Vol.32 No.2

Environmental Chemicals Inhibit Sperm Tyrosine Phosphorylation

El-Sayed A. Mohamed,Yoo-Jin Park,Woo-Sung Kwon,Sung-Jae Yoon,Kyu-Hyun Jeong,Myung-Geol Pang 한국동물생명공학회(구 한국동물번식학회) 2011 발생공학 국제심포지엄 및 학술대회 Vol.2011 No.1

Phosphorylation of proteins is a post-translational modification process which plays a significant role in a wide range of cellular processes. Addition or removal of phosphate groups result in conformational changes in proteins leading either to their activation or inactivation. Tyrosine phosphorylation of protein is associated with sperm function in several mammalian species. The control of this process may via the changes in cyclic adenosine monophosphate (cAMP); the changes in cAMP levels that occur in the spermatozoa regulate protein kinase A (PKA) activity which, in turn, leads to the tyrosine phosphorylation of protein substrates by either the activation of sperm tyrosine kinases and/or the inhibition of phosphoprotein phosphatases. Cyclic nucleotides, in particular, cAMP, are important regulators of various maturation events in sperm including capacitation and motility. Interestingly, some environmental chemicals (ECs) may exert broader endocrine disrupting effects through possible modulation of cAMP/PKA second messenger systems. Otherwise, because the mature spermatozoa are transcriptionally inactive, therefore the study of sperm proteins phosphorylation may permit more information about the agents and conditions affects on sperm function. In the present study, to examine the effect of ECs on human sperm function, human spermatozoa were incubated with a group of ECs represent a widespread chemicals in the environment bisphenol A (BPA, 100 μM), nonylphenol (NP, 10 μg/ml), 2,3,7,8-Tetrachlorodibenzo- pdioxin (TCDD, 2.5 μg/ml), genistein (Gen, 100 μM), and the following pesticides, dibromochloropropane (DBCP, 10 μg/ml), atrazine (Atraz, 500 μM), and diazinone (Diaz, 500 μM) for 6 hr at 37℃ in 5% CO2. Then, western blot analysis was carried out using extracted sperm proteins. Antiphosphorylation antibody (pY20) was used to determine sperm tyrosine phosphorylation after EDs treatment. The pY20 antibody labeled three common bands of approximately 90, 110, and 150 KDa. There were no significant differences between negative and positive control groups in regard to the tyrosine phosphorylated proteins except at the band with molecular weight 110 KDa. However, except Diaz treatment group, the other treatment groups showed decreasing (TCDD, Gen, NP, BPA, and DBCP) or increasing (Atraz) in the tyrosine phosphorylated proteins at least in one band from the three common bands studied. Therefore, it sug-gests that ECs effectively alters human sperm function and this effect may detect via their effect on tyrosine phosphorylation pattern.

FRACTIONAL DIFFERENTIAL EQUATIONS

El-Sayed, A.M.A.. Department of Mathematics 1988 Kyungpook mathematical journal Vol.28 No.2

In recent years, several authors have dealt with the fraction derivative [1], in special functions [2], convolution integral equation [5], differintegral equation [4], the derivative of H-function [6], and some other applications. The present paper considers the fraction derivatve in the form of differential eqiation.