Alveolar ridge augmentation with the perforated and nonperforated bone grafts

Erica Dorigatti de Avila,José Scarso Filho,Lizete Toledo de Oliveira Ramalho,Mario Francisco Real Gabrielli,Valfrido Antônio Pereira Filho 대한치주과학회 2014 Journal of Periodontal & Implant Science Vol.44 No.1

Purpose: Autogenous bones are frequently used because of their lack of antigenicity, butgood osteoconductive and osteoinductive properties. This study evaluated the biologicalbehavior of perforated and nonperforated cortical block bone grafts. Methods: Ten nonsmoking patients who required treatment due to severe resorption of thealveolar process and subsequent implant installation were included in the study. The inclusioncriteria was loss of one or more teeth; the presence of atrophy of the alveolar processwith the indication of reconstruction procedures to allow rehabilitation with dental implants;and the absence of systemic disease, local infection, or inflammation. The patientswere randomly divided into two groups based on whether they received a perforated (innersurface) or nonperforated graft. After a 6-month healing period, a biopsy was performedand osseointegrated implants were installed in the same procedure. Results: Fibrous connective tissue was evident at the interface in patients who receivednonperforated grafts. However, full union between the graft and host bed was visible inthose who had received a perforated graft. Conclusions: We found that cortical inner side perforations at donor sites increased thesurface area and opened the medullary cavity. Our results indicate an increased rate ofgraft incorporation in patients who received such perforated grafts.

Alveolar ridge augmentation with the perforated and nonperforated bone grafts

de Avila, Erica Dorigatti,Filho, Jose Scarso,de Oliveira Ramalho, Lizete Toledo,Real Gabrielli, Mario Francisco,Pereira Filho, Valfrido Antonio Korean Academy of Periodontology 2014 Journal of Periodontal & Implant Science Vol.44 No.1

Purpose: Autogenous bones are frequently used because of their lack of antigenicity, but good osteoconductive and osteoinductive properties. This study evaluated the biological behavior of perforated and nonperforated cortical block bone grafts. Methods: Ten nonsmoking patients who required treatment due to severe resorption of the alveolar process and subsequent implant installation were included in the study. The inclusion criteria was loss of one or more teeth; the presence of atrophy of the alveolar process with the indication of reconstruction procedures to allow rehabilitation with dental implants; and the absence of systemic disease, local infection, or inflammation. The patients were randomly divided into two groups based on whether they received a perforated (inner surface) or nonperforated graft. After a 6-month healing period, a biopsy was performed and osseointegrated implants were installed in the same procedure. Results: Fibrous connective tissue was evident at the interface in patients who received nonperforated grafts. However, full union between the graft and host bed was visible in those who had received a perforated graft. Conclusions: We found that cortical inner side perforations at donor sites increased the surface area and opened the medullary cavity. Our results indicate an increased rate of graft incorporation in patients who received such perforated grafts.

이탈리아와 독일 파견근로의 정치경제학

Chiara Benassi,Lisa Dorigatti 한국노동연구원 2018 국제노동브리프 Vol.16 No.6

An in vitro model of Fusobacterium nucleatum and Porphyromonas gingivalis in single- and dual-species biofilms

Lívia Jacovassi Tavares,Marlise Inêz Klein,Beatriz Helena Dias Panariello,Erica Dorigatti de Avila,Ana Cláudia Pavarina 대한치주과학회 2018 Journal of Periodontal & Implant Science Vol.48 No.1

Purpose: The goal of this study was to develop and validate a standardized in vitro pathogenic biofilm attached onto saliva-coated surfaces. Methods: Fusobacterium nucleatum (F. nucleatum) and Porphyromonas gingivalis (P. gingivalis) strains were grown under anaerobic conditions as single species and in dual-species cultures. Initially, the bacterial biomass was evaluated at 24 and 48 hours to determine the optimal timing for the adhesion phase onto saliva-coated polystyrene surfaces. Thereafter, biofilm development was assessed over time by crystal violet staining and scanning electron microscopy. Results: The data showed no significant difference in the overall biomass after 48 hours for P. gingivalis in single- and dual-species conditions. After adhesion, P. gingivalis in single- and dual-species biofilms accumulated a substantially higher biomass after 7 days of incubation than after 3 days, but no significant difference was found between 5 and 7 days. Although the biomass of the F. nucleatum biofilm was higher at 3 days, no difference was found at 3, 5, or 7 days of incubation. Conclusions: Polystyrene substrates from well plates work as a standard surface and provide reproducible results for in vitro biofilm models. Our biofilm model could serve as a reference point for studies investigating biofilms on different surfaces.

Facial and occlusal esthetic improvements of an adult skeletal Class III malocclusion using surgical, orthodontic, and implant treatment

Mauricio de Almeida Cardoso,Rafael Scaf de Molon,Erica Dorigatti de Avila,Fabio Pinto Guedes,Valter Antonio Ban Battilani F,Leopoldino Capelozza Filho,Marcio Aurelio Correa,Hugo Nary Filho 대한치과교정학회 2016 대한치과교정학회지 Vol.46 No.1

The aim of this clinical report is to describe the complex treatment of an adult Class III malocclusion patient who was disappointed with the outcome of a previous oral rehabilitation. Interdisciplinary treatment planning was performed with a primary indication for implant removal because of marginal bone loss and gingival recession, followed by orthodontic and surgical procedures to correct the esthetics and skeletal malocclusion. The comprehensive treatment approach included: (1) implant removal in the area of the central incisors; (2) combined orthodontic decompensation with mesial displacement and forced extrusion of the lateral incisors; (3) extraction of the lateral incisors and placement of new implants corresponding to the central incisors, which received provisional crowns; (4) orthognathic surgery for maxillary advancement to improve occlusal and facial relationships; and finally, (5) orthodontic refinement followed by definitive prosthetic rehabilitation of the maxillary central incisors and reshaping of the adjacent teeth. At the three-year follow-up, clinical and radiographic examinations showed successful replacement of the central incisors and improved skeletal and esthetic appearances. Moreover, a Class II molar relationship was obtained with an ideal overbite, overjet, and intercuspation. In conclusion, we report the successful esthetic anterior rehabilitation of a complex case in which interdisciplinary treatment planning improved facial harmony, provided gingival architecture with sufficient width and thickness, and improved smile esthetics, resulting in enhanced patient comfort and satisfaction. This clinical case report might be useful to improve facial esthetics and occlusion in patients with dentoalveolar and skeletal defects.

An in vitro model of Fusobacterium nucleatum and Porphyromonas gingivalis in single- and dual-species biofilms

Tavares, Livia Jacovassi,Klein, Marlise Inez,Panariello, Beatriz Helena Dias,de Avila, Erica Dorigatti,Pavarina, Ana Claudia Korean Academy of Periodontology 2018 Journal of Periodontal & Implant Science Vol.48 No.1

Purpose: The goal of this study was to develop and validate a standardized in vitro pathogenic biofilm attached onto saliva-coated surfaces. Methods: Fusobacterium nucleatum (F. nucleatum) and Porphyromonas gingivalis (P. gingivalis) strains were grown under anaerobic conditions as single species and in dual-species cultures. Initially, the bacterial biomass was evaluated at 24 and 48 hours to determine the optimal timing for the adhesion phase onto saliva-coated polystyrene surfaces. Thereafter, biofilm development was assessed over time by crystal violet staining and scanning electron microscopy. Results: The data showed no significant difference in the overall biomass after 48 hours for P. gingivalis in single- and dual-species conditions. After adhesion, P. gingivalis in single- and dual-species biofilms accumulated a substantially higher biomass after 7 days of incubation than after 3 days, but no significant difference was found between 5 and 7 days. Although the biomass of the F. nucleatum biofilm was higher at 3 days, no difference was found at 3, 5, or 7 days of incubation. Conclusions: Polystyrene substrates from well plates work as a standard surface and provide reproducible results for in vitro biofilm models. Our biofilm model could serve as a reference point for studies investigating biofilms on different surfaces.